• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.695-703
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• 1999

We have studied the stereospecificities of various pyridoxal 5'-phosphate (PLP) dependent enzymes for the hydrogen transfer between the C-4' of a bound coenzyme and the C-2 of a substrate in the transamination catalyzed by the enzymes. Stereospecificities reflect the structures of enzyme active-sites, in particular the geometrical relationship between the coenzyme-substrate Schiff base and the active site base participating in an $\alpha$-hydrogen abstraction. The PLP enzymes studied so far catalyze only a si-face specific (pro-S) hydrogen transfer. This stereochemical finding suggests that the PLP enzymes have the same topological active-site structures, and that the PLP enzymes have evolved divergently from a common ancestral protein. However, we found that o-amino acid aminotransferase, branched chain L-amino acid aminotransferase, and 4-amino-4-deoxychorismate lyase, which have significant sequence homology with one another, catalyze a re-face specific (pro-R) hydrogen transfer. We also showed that PLP-dependent amino acid racemases, which have no sequence homology with any aminotransferases, catalyze a non-stereospecific hydrogen transfer: the hydrogen transfer occurs on both faces of the planar intermediate. Crystallographical studies have shown that the catalytic base is situated on the re-face of the C-4' of the bound coenzyme in o-amino acid aminotransferase and branched chain L-amino acid aminotransferase, whereas the catalytic base is situated on the si-face in other aminotransferases (such as L-aspartate aminotransferase) catalyzing the si-face hydrogen transfer. Thus, we have clarified the stereospecificities of PLP enzymes in relation with the primary structures and three-dimensional structures of the enzymes. The characteristic stereospecificities of these enzymes for the hydrogen transfer suggest the convergent evolution of PLP enzymes.

• Journal of the Economic Geographical Society of Korea
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• v.15 no.2
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• pp.332-342
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• 2012

The current research aims at classifying the bus stops as transfer center in order to establish the rational bus transfer systems. Existing research typically identifies characteristics of demands for bus stops and land use surrounding the bus stops and classifies and profiles the bus stops. A common problem with this type of research is that the results with cross-sectional characteristics of land use and bus stop usage do not capture the details of trip chain, the fundamental characteristics of the trips with transfer. This paper therefore examines bus stop classifications with such variables as transport mode chains, intermediate stop chains and timing chains. The analysis on the data collected on Monday 20 April 2009 for passengers of Gyeong-gi bus results in a clear classification among bus stops in terms of such trip chain variables. The research would provide useful information for the decision support of transfer stops location choice and infrastructure design.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.05a
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• pp.157-158
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• 2021

Big data in the supply chain mainly comes from four aspects. One is the relevant data inevitably generated in the process of product value transfer of enterprises in the supply chain, such as production equipment quality data, planned procurement data, product data, etc; On the other hand, it is derived from the ERP data of various companies in the supply chain; The third is e-commerce data from the customer, and the last is data from external or manually entered data. A third-party data service center analysis and mining the data to predict and control the inventory in the process of supply chain operation. It brings innovation and change of management technology and way of thinking to the whole supply chain in many aspects, and finally achieves the goal of coordinated inventory and zero inventory of the whole supply chain.

• Journal of Embryo Transfer
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• v.15 no.3
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• pp.219-230
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• 2000

This study was carried out to determine the factors on achieving good viability of embryos biopsied fur sexing, to investigate pregnancy rate following embryo transfer(ET) with sexed embryos, and to confirm the accuracy for the calves bort following ET with sexed embryos by polymerase chain reaction(PCR). To investigate viability of Hanwoo embryos after biopsy for sexing, fresh and frozen/thawed embryos were biopsied according to different developmental day of blastocysts, different stage of blastocysts, and different biopsy grade and the embryos themselves were incubated for 2 hours in TCM199 after microsection to be evaluated morphologically for recovery as blastocyst. The results obtained were as follows : 1. The rate of oocytes cleaved in vitro and the rate of blastocyst of the cleaved oocytes were 52.5% and 21.6%, respectively. The rate of blastocyst on day 8 was 11.2%, denoting the highest rate during whole culture period posterior to in vitro fertilization(IVF) 2. After biopsy for sexing, the viability rate of blastocyst on day 7, 8 and 9 was 75.0%, 88.4%, and 100.0%, respectively and the viability of early, mid, and expanded blastocyst after biopsy was 75.0%, 88.9%, and 91.1%, respectively The viability rate of fresh and frozen/thawed embryos was 89.9%, 71.4%, respectively. And the viability of expanded, hatching, and hatched blastocyst of frozen/thawed embryos was : 75.0%, 75.0%, and 50.0%, respectively. The viability of embryos according to biopsy grade of 10∼20%, 21∼30%, and 31∼40% was 85.7%, 91.5%, and 71.4%, respectively. 3. Pregnancy rate after transfer with biopsied embryo between flesh and frozen/thawed embryos was 22.6% and 20.0%, respectively. 4. In comparison between sex by PCR method and sex of calves born after embryo transfer, the accuracy of sex deterimination was 92.3% (12/13).

• Journal of Radiation Protection and Research
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• v.16 no.2
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• pp.55-65
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• 1991

Soil-to-plant transfer coefficients of Mn-54, Co-60, Zn-65, and Cs-137 were estimated for the edible parts of the rice, soybean, lettuce, carrot, and squash grown in different soils by radiotracer uptake experiments using pot cultures. The transfer coefficients of radionuclides were in the order of Zn-65 > Mn-54 > Cs-137 > Co-60 in most of the cases studied. The coefficients for soybean were roughly an order of magnitude higher than those for rice. Among vegetables, lettuce mostly showed the highest value and squash, the lowest. In the strongly acidic soils, transfer coefficients were much higher than in the moderately acidic soils. From the data obtained. crop-specific transfer coefficients of the four nuclides were proposed for the use in Korean food-chain radiation dose assessment.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.322-322
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• 2006

Well-defined poly(N-vinylcarbazole), poly(NVC), was synthesized by macromolecular design via interchange of the xanthate (MADIX)/reversible addition-fragmentation chain transfer (RAFT) polymerization in the presence of a suitable xanthate-type chain transfer agent (CTA). Good control of the polymerization was confirmed by the linear first-order kinetic plot, the molecular weight controlled by the monomer/CTA molar ratio, linear increase in the molecular weight with the conversion, and the ability to extend the chains by the second addition of the monomer. Star polymers having various architectures were also synthesized using this technique.

• Journal of the Korean Chemical Society
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• v.16 no.1
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• pp.46-49
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• 1972

Using an assumption of isoviscosity at the gel-point in the polymerization of divinylbenzene, the relations between viscosity equation, viscosity-molecular weight, and the chain transfer equation have been studied. A new equation for the calculation of chain transfer constant, $C_{tr}$, by measuring the gel time in place of the degree of polymerization has been suggested.

• Macromolecular Research
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• v.13 no.3
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• pp.236-242
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• 2005

Three dithioester-derived carboxyl acid functionalized RAFT(reversible addition-fragmentation chain transfer) agents, viz. acetic acid dithiobenzoate, butanoic acid dithiobenzoate and 4-toluic acid dithiobenzoate, were used in the RAFT bulk polymerization of styrene, in order to study the effects of the R-group structure on the living nature of the polymerization. By conducting the polymerization with various concentrations of the RAFT agents and at different temperatures, it was found that the R-group structure of the RAFT agents plays an important role in the RAFT polymerization; the bulky structure and radical stabilizing property of the R-group enhances the living nature of the polymerization and allows the polymerization characteristics to be well controlled.

• Bulletin of the Korean Chemical Society
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• v.31 no.10
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• pp.2943-2948
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• 2010

Silica monolith powders were prepared by a new procedure where ground powders of proper size distribution were obtained without sieving. An initiator was attached to this ground monolith and polystyrene was bound by reversible addition-fragmentation chain transfer polymerization to give a new stationary phase. The separation efficiency of this phase was found better than that of the polystyrene bound phase based on conventional silica particles and that of the C18 bound silica monolith powders.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2002.03a
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• pp.89-99
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• 2002

Since 1996, the document exchange method by EDI has been introduced in port and logistics industries in Korea to enhance the declaration activities to the Pusan Port Authority and Customs. In spite of these efforts, users such as shipping companies, shipping agents, and freight forwarders have complained the inconvenience of using EDI systems. The major reasons can be summarized as follows: repetitional EDI transfer, too much transfer time, inconvenient EDI software, and problems on message receiving confirmation. Furthermore, EDI has been used only for transmitting messages. To solve these problems, this study has adopted the integration system by applying the concept of SCM to logistics system by XML/EDl. In the view of SCM, the existing EDI has some problems because its role is only to transmit the messages. This paper is to suggest how current EDI systems in Port and logistics sector has to be chanced by Introducing SCM. The integration system gives us such advantages as accuracy of cargo data, integration of processes among firms, and keeping the trace of cargo.