• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.77-84
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• 2003

Chitinase was purified from an antagonistic bacterium Bacillus sp. 7079 by ammonium sulfate precipitation, QAE-Sephadex anion exchange chromatography, Sephadex G-100 gel filtration, and SP-Sephadex cation exchange chromatography. The molecula. weight of purified chitinase (PC-1) was approximately 66.5 kDa on SDS-PACE. PC-1 exhibited optimum pH and temperature of pH 7.5 and $45^{\circ}C$, respectively. More than $80\%$ of PC-1 was stable at pH 5.0 to 9.0, and more than $90\%$ at $40^{\circ}C$. $Fe^2+\;and\;Ca^2+$ inhibited the chitinase activity about $20\%$, and EDTA and p-CMB by about $30\%$, whereas $Ag^+$ inhibited the activity up to $65\%$. The $K_m$ value of PC-1 was 1.215 mg/ml with colloidal chitin as a substrate. We also investigated the effect of PC-1 treated chitin (PCTC) on the pro-inflammatory cytokine gene expression in macrophage RAW 264.7 cells. The expression of IL-$1{\alpha}$ and IL-$1{\beta}$ mRNA gene was investigated using reverse transcriptase polymerase chain reaction (RT-PCR). IL-$1{\alpha}$ and IL-$1{\beta}$ mRNA were induced by the treatment of PCTC and chitin only in RAW 264.7 cells. These expressions were induced as early as 2 h and sustained up to 24 h in RAW 264.7 cells. IL-$1{\alpha}$ and IL-$1{\beta}$ mRNA were more strongly expressed by the treatment of PCTC than chitin treatment alone in RAW 264.7 cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.3
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• pp.771-776
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• 2003

The present study was conducted to evaluate the regulation mechanism of nitric oxide(NO) by water-extracted Cyperus rotundus (WCR) in RAW 264.7 macrophages. We investigated the effects of cell proliferation in mouse spleen cell and RAW 264.7 macrophages cells. WCR enhanced mitogenic activity in the dose-response manner in mouse spleen cells and RAW 264.7 macrophages cells. In nitric oxide (NO) synthesis by WCR, WCR alone had an effect on NO synthesis. It was found that the production of NO of RAW 264.7 cells stimulated with lipopolysaccharide (LPS) could be markedly inhibited by WCR. Inhibition of NO production was achieved by reducing inducible nitric oxide syntheses(iNOS) mRNA expression. The expression of IL-I gene by WCR was investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, IL-1 family(IL-1 α, IL-1β) expressions were induced by WCR. These finding suggested that regulation of NO production by WCR may be, at least in part, associated with the regulation of iNOS mRNA expression and IL-1 family gene expression.

• The Plant Pathology Journal
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• v.28 no.4
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• pp.357-363
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• 2012

Several species belonging to the Gibberella fujikuroi species (Gf ) complex are commonly associated with rice and corn, not only causing serious diseases, but also producing fumonisins, a group of mycotoxins harmful to animals and humans. To characterize the population structure of the putative fumonisin-producing Gf complex in Korea, we obtained 276 candidate isolates from rice and corn harvested in 2009 and 2010 by diagnostic polymerase chain reaction with several specific primer sets. Phylogenetic trees were constructed using multilocus sequences (combined RPB2 and EF1A, totaling 1.6 kb) from these isolates. Among the 135 isolates from rice, F. fujikuroi (teleomorph: G. fujikuroi; 59.3%) and F. proliferatum (G. intermedia; 13.3%) were predominant, followed by F. concentricum (5.9%). Additionally, twenty-five (18.5%) rice isolates belonged in a distinct subclade of F. commune, a non-member of the Gf complex. In contrast, F. verticillioides was the most predominant species (38.3%) among the 141 corn isolates, and followed by F. fujikuroi (27.7%), F. proliferatum (14.9%), F. subglutinans (7.1%), and F. concentricum (2.8%). A single mating type (MAT1-1) was found predominantly among the Gf complex isolates examined. Possible distinct subclades were detected within the populations of F. fujikuroi and F. proliferatum; however, this needs further confirmation. This is the first reported population-level characterization of putative fumonisin-producing Gf complex associated with rice and corn in Korea.

• Korean Journal of Veterinary Service
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• v.28 no.3
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• pp.225-234
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• 2005

Egg has been considered as one of the most important food sources in both nutritionally and economically. However, because the hazards of egg flow chains' complexity from producers to consumers and the insufficiency of cold chain systems in Korea are still remained, preventive measures for egg safety should be established. This experiment was carried out to investigate the changes of egg qualify during storage in order to find out the proper storage condition and eggshell treatment. Eggs were stored at cold condition $(5^{\circ}C,\;relative\;humidity;\;RH:\;65\pm3\%)$ and room Condition $(1st: Sep\; 6\~Nov\; 15,\;2001,\; 10\~30^{\circ}C,\;RH\;41\~86\%,\;2nd;\; Dec\;13,\;2001\~Feb\;21,\;2002,\;4\~23^{\circ}C,\;RH\;36\~89\%)$ after eggshell treatment (unwashed, washed and coated egg) during'10 weeks and examined weight loss and interior quality periodically. Weight loss was in decreasing order room washed > room unwashed > cold washed > cold unwashed > room coated > cold coated egg. And, Haugh unit was decreasing order room washed > room unwashed > room coated > cold washed > cold unwashed > cold coated egg. Our findings show that both coated eggs and refrigeration reduces the rate of decline in Haugh units and egg weight. And, washed eggs had lower average Haugh units and a higher weight loss than any of the eggshell treatment in both storage conditions. Conclusively, for washing eggshell, 150 ppm of sodium hypochlorite, regular exchange and temperature control of water should be consistent and also eggs should be dried immediately after washing. Also, national guidelines for temperature and moisture of storage places, transportation and expiration data should be established to keep quality and weight loss of eggs. Storage temperature that is a critical factor in the quality and safety of eggs should be kept under 12'c and labelling of expiration date should be obligatory.

• Journal of Auto-vehicle Safety Association
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• v.11 no.4
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• pp.16-21
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• 2019

In recent years, most automobile manufacturers have been using slim light source images to imprint their own brand image and identity. A light guide type lamp is widely used for making these kinds of a slim light source image. A light guide lamp means using a light emitting diode light source at one end of a long cylindrical pipe. The light from the light emitting diode source moves through the pipe by total reflection principle. Moving light is sent forward by various optic structures which is applied in the cylindrical pipe. However, the light guide lamp has a problem that the image of the light differs depending on the viewing direction, and in some cases there is dark section. It means light guide has low quality about lighting image. In this paper, trying to improve the fundamental problems of the light guide mentioned above by using various triz methods. Through functional modeling, estimating the factors affecting the light in the light guide lamp and make various ideas to improve the lighting image using the chain effect cause analysis, function oriented search scientific database techniques. Using these kinds of various TRIZ methods, finally find solutions that can improve the brightness and lighting uniformity of the light guide lamp. The ideas obtained in this paper were applied to actual vehicle development, and several patents achievements were obtained. In conclusion, it is proved that TRIZ method is useful for making ideas in actual automobile industrial field and is also a useful method for acquiring patent.

• Journal of the Korea Safety Management & Science
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• v.17 no.2
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• pp.215-228
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• 2015

A creative innovation and an innovative problem-solving of industrial companies can be achieved by overcoming the challenges of technical and physical contradictions. The approaches to address conflicting and paradoxical problems, such as technical and physical contradictions have a crucial role in advancing the quality assessment for manufacturer and service provider. The term, technical contradiction, depicts the state that improvement of one ends of IFR (Ideal Final Result) leads to unfavorable condition of the other ends, and results in conflicting problem. Another type of contradictions that's discussed in this study is a physical contradiction which is due to two mutually opposing states of the means of ends, and gives paradoxical situation. By integrating the means-ends chain perspectives, the physical contradiction that is a specifically root-causes, "means", can be initially addressed to resolve the downstream problem of technical contradiction which represents a general and abstract goals, "ends". This research suggests IFR resolution processes to handle both physical contradiction of means and technical contradiction of ends by employing causal relationship with IFR, effects and causes. In summary, the study represents three major processes that resolve such contradictions are demonstrated as follows: 1) Derivation of causal and hierarchical relationship among IFR, ends and means by considering CAED (Cause-And-Effect Diagram) and LT (Logic Tree). 2) Identification of causal relationship between physical contradiction and technical contradiction by using TPCT (TRIZ Physical Contradiction Tree) and TCD (Technical Contradiction Diagram). 3) Application of integrated TRIZ principles by classifying 40 inventive principles into 4 general conditions of the separation principle of mutually opposite states in space, in time, based on conditions, and between the parts and the whole. In order to validate the proof of proposed IFR resolution processes, the analysis of the TRIZ case studies from National Quality Circle Contest in the years, 2011 to 2014 have been proposed. The suggested guidelines that are built based on TRIZ principles can uniquely enhance the process of quality innovation and assessment for quality practitioners.

• Journal of the Korea Safety Management & Science
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• v.5 no.3
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• pp.81-98
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• 2003

In modern society, there are diverse sorts of VIPs including a king and individuals. Some like a king or president are in need of professional secret services, and others become VIPs by being guarded by a bodyguard company at their own request. The former is called a public guard, and the latter is a private one. In the field of the public guard, the nation should be furnished with a guard system in consideration of political circumstances to protect VIPs. That is, the parliamentary cabinet system and presidential system call for a different guard system, and the guard system's professional features vary to some degree with political situations and cultural climate. If the ultimate goal of guard is to protect the chief of the democratic and open state in a most efficient and safe manner, it's mandatorily necessary to set up a professional and specialized guard organization to provide democratic and efficient secret services. It's desired that the domestic presidential guard division should be staffed with not only expert guards but other personnels dispatched from the military and police. In the police, guard mission is splitted among various guard and security agencies, including the National Police Agency's public security bureau or the Seoul Metropolitan Police Agency's public security division, 101 security unit, mobile police and 22 special guard division. As a chain of command is separated and it's difficult to manage such organizations effectively, the National Police Agency should be exclusively in charge of VIP guard and security. In the area of private guard, the private police system has shared national security task as a middle organization between the private police and private guard. But now its intent has gradually weakened, and its mission should be redefined. In addition, the way people look at private guard should change, and they should be well informed that it could serve to prevent possible crimes, protect them against crimes and get rid of their inconveniences. To make a good impression on people, the guard associations and businesses should put their efforts into reinforcing publicity activities, and the government, academic circles and press should team up with them as well.

• Journal of the Korea Safety Management & Science
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• v.14 no.4
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• pp.153-168
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• 2012

It has been recognized as an important issue to design optimally a firm's logistics network for minimizing logistics cost and maximizing customer service. It is, however, not easy to get an optimal solution by analyzing trade-off of cost factors, dynamic and interdependent characteristics in the logistics network decision making. Although there has been some developments in a system which helps decision making for logistics analysis, it is true that there is no system for enterprise-wise's on-site support and methodical logistics decision. Specially, E-biz process along with information technology has been made dramatic advance in a various industries, there has been much need for practical education closely resembles on-site work. The software developed by this study materializes efficient algorithm suggested by recent studies in key topics of logistics such as location and allocation problem, traveling salesman problem, and vehicle routing problem and transportation and distribution problem. It also supports executing a variety of experimental design and analysis in a way of the most user friendly based on Java. In the near future, we expect that it can be extended to integrated supply chain solution by adding decision making in production in addition to a decision in logistics.

• Journal of Convergence for Information Technology
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• v.9 no.6
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• pp.7-12
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• 2019

In order to shoot underwater, the photographer must be equipped with shooting equipment and enter into the water. Since the photographer directly enters the water, safety accidents occur frequently due to various obstacles or deep water in the water. The proposed underwater stereo photography technique can solve the safety accident problem caused by the entry of the photographer into the water by using the drone for underwater photographing. In addition, this technique has the advantage of obtaining underwater images at low cost. In this study, the angle of the proposed cam for stereoscopic photography was analyzed and the condition that the proper stereoscopic image can be viewed was defined as the distance from the floor of 18cm to the floor distance of 41.4cm. This provision is proposed to be used to adjust the height of the shooting area descended by the elevation chain of the water surface hovering drones.

• Laboraroty Animal Research
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• v.34 no.4
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• pp.232-238
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• 2018

Animal models have been used to elucidate the pathophysiology of varying diseases and to provide insight into potential targets for therapeutic intervention. Although alternatives to animal testing have been proposed to help overcome potential drawbacks related to animal experiments and avoid ethical issues, their use remains vital for the testing of new drug candidates and to identify the most effective strategies for therapeutic intervention. Particularly, the study of metabolic diseases requires the use of animal models to monitor whole-body physiology. In line with this, the National Institute of Food and Drug Safety Evaluation (NIFDS) in Korea has established their own animal strains to help evaluate both efficacy and safety during new drug development. The objective of this study was to characterize the response of C57BL/6NKorl mice from the NIFDS compared with that of other mice originating from the USA and Japan in a chemical-induced diabetic condition. Multiple low-dose treatments with streptozotocin were used to generate a type-1 diabetic animal model which is closely linked to the known clinical pathology of this disease. There were no significantly different responses observed between the varying streptozotocin-induced type-1 diabetic models tested in this study. When comparing control and diabetic mice, increases in liver weight and disturbances in serum amino acids levels of diabetic mice were most remarkable. Although the relationship between type-1 diabetes and BCAA has not been elucidated in this study, the results, which reveal a characteristic increase in diabetic mice of all origins are considered worthy of further study.