• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.4
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• pp.359-369
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• 2007

Amoebophrya is an obligate endoparasitic eukaryotic dinoflagellate infecting host species and eventually killing them within a short period. Because of its host specificity and significant impacts on population dynamics of host species, it has long been proposed to be a potential biological agent for controlling harmful algal bloom (HAB). For several decades, the difficulties of culturing host - parasite systems have been a great obstacle to further research on the biology of Amoebophrya but recent success of several culture systems reactivates this research field. In this study, as a preliminary work for understanding the impacts of Amoebophrya on the population dynamics of host species, semimonthly occurrence of infected host dinoflagellates by Amoebophrya spp. had been observed in Jinhae Bay for two years and with a host - parasite system cultivated, host specificity of Amoebophrya spp. on several dinoflagellates was tested. Amoebophrya spp. were observed in the cellular organelle and cytoplasm of several species including Akashiwo sanguinea, Ceratium fusus, Dinophysis acuminata, Heterocapsa triquetra, Oblea sp., Prorocentrum minimum, P. triestinum, Scrippsiella spinifera, and S. trochoidea. Among them two host - parasite systems for an athecate dinoflagellate, A. sanguinea, and for a thecate dinoflagellate, H. triquetra, had been able to be successfully established as laboratary cultures. Cross-infection tests for 6 species of dinoflagellates in which Amoebophrya was observed or had been reported to exist confirmed high preference for host species of the parasite. Through the continuous research on Amoebophrya occurring in Korean coastal waters, we need to maintain various host - parasite culture systems, which will be very helpful for understanding its ecological role in marine food webs and for applying the species to biologically control harmful algal blooms.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.5
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• pp.446-457
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• 1993

Monthly distribution of marine bacteria and phytoplankton in Suyeong Bay were investigated with laboratory experiment of dominant algal species and bacterial flora. During the periods of study, the highest density of phytoplankton and bacteria occurred in May with the number of $3.3{\times}10^6cells/l$ and $1.93{\times}10^8cells/ml$, respectively. 10 genera of bacteria and 22 genera of phytoplankton were isolated and identified. In May when phytoplankton bloom occurred, dominant species of bacteria were Acinetobacter calcoaceticus($29.1\%$) and Bacillus subtilis($22.9\%$), and dominant species of phytoplankton were Chaetoceros spp.($62.8\%$) and Skeletonema spp. ($19.4\%$). Pseudomonas spp., which was the most abundant bacterial species during the study periods, were rapidly decreased in May. In laboratory studies of culturing bacteria and phytoplankton isloated in May, the growth of Pseudomonas vesicularis seems to be influenced by the concentrations of excretion matter of Chaetoceros spp. To examine the result colsely, the problem of pure isolation for phytoplankton must be solved and more experimental process have to be conducted.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.1
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• pp.43-50
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• 2000

The obseuations on the seasonal fluctuations of phytoplankton community in Haechang Bay of the Korean southern sea were carried out during four seasons from 1997 to 1998. A total of 77 species of phytoplankton including 10 freshwater species, belonging to 51 Benera was identified. Seasonal succession of dominant species was evident in Haechang Bay; Chaetoceros cunisetus, Skeletonema costatum, Eurampia zodiacus, Dictyocha fibula and Ceratium furca in summer, C. curisetus in autunm, C. cunisetus Rhizosoienia setigera and E. zodiacus in winter and S. costatum in spring. The phytoplankton community in Haechang Bay showed various species composition and was occupied with centric diatoms all the year round, Densities of the phytoplankton cell number by the samples of Haechang Bay ranged from $8.4{\times}10^3\;cells/l\;to\;2.0{\times}10^5\;cells/l$ with the mean value of $9.2{\times}10^4\;cells/l$ in summer, from $3.2{\times}10^3\;cells/l\;to\;4.6{\times}10^6\;cells/l$ with mean of $6,2{\times}10^4\;cells/l$ in autumn, from $8.4{\times}10^3\;cells/l\;to\;4.3{\times}10^4\;cells/l$ with mean $2.2{\times}10^4\;cells/l$ in winter and from $1.0{\times}\;10^3\;cells/l to\;4.6{\times}10^4\;cells/l$, with mean of $1.1{\times}10^4\;cells/l$ in spring. Phytoplankton standing crops fluctuated with an annual mean of $4.7{\times}10^4 cells/l$ between the lowest value of $1.0{\times}10^3\;cells/l$ in spring and the highest value of $4.6{\times}10^5 cells/l$ in autumn, That is, phytoplankton standing crops was high in summer and autumn, while it was very low in winter and spring.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.2
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• pp.129-139
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• 2005

We investigated the characteristics of the thermohaline structure and phytoplankton community from the southwestern areas of Jeju to the nothern areas of Taiwan in the Ease China Sea, in June 2004. According to the analysis of a T-S diagram, three characteristic of water masses were identified. We classified them into the mixed water mass by the Chiness continental coastal waters and Yellow Sea cold water (Region A), Chinese continental coastal waters (Region B) and Taiwan warm current (Region C). Region A was characterized by low temperature, low salinity, high density and high Chl-a concentration. Region B was characterized by high temperature, low salinity, low density and high Chl-a and Region C was characterized high temperature, high salinity, low density and low Chl-a concentration. The phytoplankton community identified a total of 56 species belonging to 31 genera. The dominant species was mainly dinoflagellates, Gymnodinium breve, Scrippsiella trochoidea, Ceratium fusus, Prororcentrum triestinum, centric diatoms, Chaetoceros lorenzianus, Leptocylindrus danicus, Proboscia alata, Skeletonema costatum and pennate diatoms, Pseudonitzschia pungens, Cylidrotheca closterium. Standing crops of phytoplakton fluctuated between $0.1{\times}10^2$ cells/L and $5.7{\times}10^4$ cells/L by dominance of dinoflagellates. In the phytoplankton community, the Region A was characterized by the various species composition in 39 species, the dominint species with di-atomes, Pn. pungen, Ch. lorenzianus and standing crops from 6.9 cells/$m\ell$ to 56.6 cells/$m\ell$, Region B by the various species composition in 37 species, the dominant species with dinoflagellates, G.breve, S. trochoidea and standing crops from 4.6 cells/$m\ell$ to 26.7 cells/$m\ell$, and the Region C by low species number with 28 species, the dominant species with one dinoflagellate, S.trochoidea and one diatom, L.danicus and very low standing crops from 0.1 cells/$m\ell$ to 5.7 cells/$m\ell$. Phytoplankton productivity in the East China Sea was controlled by Chinese continental coastal waters which include a high concentrations of nutrients.

• Ocean and Polar Research
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• v.35 no.2
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• pp.157-170
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• 2013

We investigated the seasonal succession of phytoplankton assemblages in the eastern part of the South Sea of Korea in relation to surface water masses. The study areas are under the direct influence of the Tsushima Warm Current (TCW) throughout the whole year, with its strength known to be seasonally variable. The region is also influenced by coastal waters (CW) driven from the South Sea of Korea and East China Sea, particularly in summer, as indicated by low salinity in the surface water. Nutrient property of the TCW can reveals whether the origin of the TCW is the nutrient-rich Kuroshio Current or the oligotropic Taiwan Warm Current. Surface chlorophyll-a (Chl-a) concentrations displayed a large seasonal variation for all stations, with high values found in spring and autumn and low values in summer and winter. At station M (offshore) and P (intermediate location between M and R), Chl-a concentrations in October were higher than those in March, when spring bloom normally occurs. This may be related to deeper mixed layer depths in October. Diatoms dominated under conditions of high nutrient supply in which Chaetoceros spp. and Skeletonema costatum-like spp. were abundant. S. costatum-like spp. dominated at stations R (onshore station) and P in December when there was greater nutrient supply, especially of phosphate. Flagellates and dinoflagellates dominated at all three stations after diatoms blooms. Dominant species were Scrippsiella trochoid in April and Ceratium furca in October at station R, and Gyrodinium spp. and Gymnodinium spp. at station M during summer, when the effect of the oligotropic Taiwan Warm Current and the oligotropic coastal water from East China Sea were strong. Redundancy analysis showed clear seasonal successions in the phytoplankton community and environmental conditions, in which both principal components 1 and 2 accounted for 69.6% of total variance. Our results suggested that environmental conditions seemed to be determined by the origin of the TCW and the relative seasonal strength of the water masses of the TCW and CW, which may affect phytoplankton growth and compositions in the study area.

• ALGAE
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• v.32 no.3
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• pp.199-222
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• 2017

Occurrence of Cochlodinium polykrikoides red tides have resulted in considerable economic losses in the aquaculture industry in many countries, and thus predicting the process of C. polykrikoides red tides is a critical step toward minimizing those losses. Models predicting red tide dynamics define mortality due to predation as one of the most important parameters. To investigate the roles of heterotrophic protists in red tide dynamics in the South Sea of Korea, the abundances of heterotrophic dinoflagellates (HTDs), tintinnid ciliates (TCs), and naked ciliates (NCs) were measured over one- or two-week intervals from May to Nov 2014. In addition, the grazing impacts of dominant heterotrophic protists on each red tide species were estimated by combining field data on red tide species abundances and dominant heterotrophic protist grazers with data obtained from the literature concerning ingestion rates of the grazers on red tide species. The abundances of HTDs, TCs, and NCs over the course of this study were high during or after red tides, with maximum abundances of 82, 49, and $35cells\;mL^{-1}$, respectively. In general, the dominant heterotrophic protists differed when different species caused red tides. The HTDs Polykrikos spp. and NCs were abundant during or after C. polykrikoides red tides. The mean and maximum calculated grazing coefficients of Polykrikos spp. and NCs on populations of co-occurring C. polykrikoides were $1.63d^{-1}$ and $12.92d^{-1}$, respectively. Moreover, during or after red tides dominated by the phototrophic dinoflagellates Prorocentrum donghaiense, Ceratium furca, and Alexandrium fraterculus, which formed serial red tides prior to the occurrence of C. polykrikoides red tides, the HTDs Gyrodinium spp., Polykrikos spp., and Gyrodinium spp., respectively were abundant. The maximum calculated grazing coefficients attributable to dominant heterotrophic protists on co-occurring P. donghaiense, C. furca, and A. fraterculus were 13.12, 4.13, and $2.00d^{-1}$, respectively. Thus, heterotrophic protists may sometimes have considerable potential grazing impacts on populations of these four red tide species in the study area.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.4
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• pp.303-309
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• 1990

To provide essential information of the role of marine bacteria on the dinoflagellate blooms, distribution of marine bacterial flora and dinoflagellate species was investigated in Chinhae Bay located in southern part of Korea from August 1989 to April 1990. Two hundred and fifty one strains of marine bacteria were isolated from seawater samples collected from the study area. Among them, Flavobacterium spp. and Acinetobacter spp. were the most dominant in bacterial flora. Another 32 strains which comprised 13 percent of total strains were Erythrobacter spp.. Based on the physiological character, Erythrobacter spp. were identified as Erythrobacter longus, Erythrobacter sp.(J-2) and Erythrobacter sp. (J-8). From the phytoplanktonic community, fourteen genera and twenty nine taxa of dinoflagellate species were identified. Based on the spatio-temporal frequence and abundance Gymnodinium sanguneum, Prorocentrum micans and Prorocentrum minimum were the aestival dominent species. However, Heterocapsa triquetra was appeared as predominant species in April. Cell density of about 2,000 cells/ml was prevailed in the bloom of August, but it developed into more intensive bloom of above 500 cells/ml in September. The water quality showed eutrophic or hypereutrophic condition, which was proved by high concentration of dissolved inorganic nitrogen, ortho-phosphate and chemical oxygen demand. Oxygen deficient water mass was found in the bottom overlying waters in August and September. High relationship between abundant bacterial flora and persistent dinoflagellate blooms in eutrophic condition would be approvable.

• ALGAE
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• v.32 no.2
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• pp.101-130
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• 2017

The ichthyotoxic Cochlodinium polykrikoides red tides have caused great economic losses in the aquaculture industry in the waters of Korea and other countries. Predicting outbreak of C. polykrikoides red tides 1-2 weeks in advance is a critical step in minimizing losses. In the South Sea of Korea, large C. polykrikoides red tide patches have often been recorded offshore and transported to nearshore waters. To explore the processes of offshore C. polykrikoides red tides, temporal variations in 3-dimensional (3-D) distributions of red tide organisms and environmental parameters were investigated by analyzing 4,432 water samples collected from 2-5 depths of 60 stations in the South Sea, Korea 16 times from May to Nov, 2014. In the study area, the vegetative cells of C. polykrikoides were found as early as May 7, but C. polykrikoides red tide patches were observed from Aug 21 until Oct 9. Cochlodinium red tides occurred in both inner and outer stations. Prior to the occurrence of large C. polykrikoides red tides, the phototrophic dinoflagellates Prorocentrum donghaiense (Jun 12 to Jul 11), Ceratium furca (Jul 11 to Aug 21), and Alexandrium fraterculus (Aug 21) formed red tides in sequence, and diatom red tides formed 2-3 times without a certain distinct pattern. The temperature for the optimal growth of these four red tide dinoflagellates is known to be similar. Thus, the sequence of the maximum growth rates of P. donghaiense > C. furca > A. fraterculus > C. polykrikoides may be partially responsible for this sequence of red tides in the inner stations following high nutrients input in the surface waters because of heavy rains. Furthermore, Cochlodinium red tides formed and persisted at the outer stations when $NO_3$ concentrations of the surface waters were < $2{\mu}M$ and thermocline depths were >20 m with the retreat of deep cold waters, and the abundance of the competing red-tide species was relatively low. The sequence of the maximum swimming speeds and thus potential reachable depths of C. polykrikoides > A. fraterculus > C. furca > P. donghaiense may be responsible for the large C. polykrikoides red tides after the small blooms of the other dinoflagellates. Thus, C. polykrikoides is likely to outgrow over the competitors at the outer stations by descending to depths >20 m and taking nutrients up from deep cold waters. Thus, to predict the process of Cochlodinium red tides in the study area, temporal variations in 3-D distributions of red tide organisms and environmental parameters showing major nutrient sources, formation and depth of thermoclines, intrusion and retreat of deep cold waters, and the abundance of competing red tide species should be well understood.

• Journal of Aquaculture
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• v.8 no.1
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• pp.47-58
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• 1995

Field studies on the seasonal succesion of phytoplankton population were carried out at the 25 stations of the breeding ground in Kogum-sudo, Southern coast of Korean peninsula in Feburuary, April, August and October, 1993. Sixty four species belonging to 40 genera were identified. Predominant species were mainly centric diatoms throughout the four seasons, two centric diatoms, Skeletonema costatum and Thalassiosira sp. and a pennate diatom, Thaiassionema nitzschioides in the winter; two pennate diatoms, Thaiassionema nitzschioides and Asterionella kariana, and especially a dinoflagellate, Heterocapsa triquetra (station 10) in the spring, two centric diatoms, S. costatum and Chaetoceros diadema in the summer; and a centric diatom, Rhizosolenia alata and a pennate diatom, Bacillaria paxillifer in the fall. The main red tide organisms in the breeding ground were dinoflagellates, Prorocentrum dentatum, P. minimum, P. triestinum, Ceratium furro, Gymnodinium sanguineum, Noctiluca scintillans, H. triquetra, Scrippsiella trichoidea and a diatom S. costatum in the Kogum Sudo. Seasonal phytoplankton cell numbers were in a wide range between $8.8\times10^3$ cells/l and 1.4\times10^6$ cells/l; The seasonal average cell numbers were $12.2\times10^4\pm5.9\times10^4$ cells/l $(mean\;\pm\;standard\; diviation)$ in the winter, $3.3\times10^4\pm1.4\times10^4$ cells/l in the spring, $48.4X10^4\pm40.0\pm10^4$ cells/l in the summer, and $3.6\times10^4\pm1.9\times10^4$ cells/l in the fall, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.1
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• pp.1-25
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• 1982

Limnological study on the physico-chemical properties and biological characteristics of the Lake Ok-Jeong was made from May 1980 to August 1981. For the planktonic organisms in the lake, species composition, seasonal change and diurnal vertical distribution based on the monthly plankton samples were investigated in conjunction with the physico-chemical properties of the body of water in the lake. Analysis of temperature revealed that there were three distinctive periods in terms of vertical mixing of the water column. During the winter season (November-March) the vertical column was completely mixed, and no temperature gradient was observed. In February temperature of the whole column from the surface to the bottom was $3.5^{\circ}C$, which was the minimum value. With seasonal warming in spring, surface water forms thermoclines at the depth of 0-10 m from April to June. In summer (July-October) the surface mixing layer was deepened to form a strong thermocline at the depth of 15-25 m. At this time surface water reached up to $28.2^{\circ}C$ in August, accompanied by a significant increase in the temperature of bottom layer. Maximum bottom temperature was $r5^{\circ}C$ which occurred in September, thus showing that this lake keeps a significant turbulence Aehgh the hypolimnial layer. As autumn cooling proceeded summer stratification was destroyed from the end of October resulting in vertical mixing. In surface layer seasonal changes of pH were within the range from 6.8 in January to 9.0 in guutuost. Thighest value observed in August was mainly due to the photosynthetic activity of the phytoplankton. In the surface layer DO was always saturated throughout the year. Particularly in winter (January-April) the surface water was oversaturated (Max. 15.2 ppm in March). Vertical variation of DO was not remarkable, and bottom water was fairly well oxygenated. Transparency was closely related to the phytoplankton bloom. The highest value (4.6 m) was recorded in February when the primary production was low. During summer transparency decreased hand the lowest value (0.9 m) was recorded in August. It is mainly due to the dense blooming of gnabaena spiroides var. crassa in the surface layer. A. The amount of inorganic matters (Ca, Mg, Fe) reveals that Lake Ok-Jeong is classified as a soft-water lake. The amount of Cl, $NO_3-N$ and COD in 1981 was slightly higher than those in 1980. Heavy metals (Zn, Cu, Pb, Cd and Hg) were not detectable throughout the study period. During the study period 107 species of planktonic organisms representing 72 genera were identified. They include 12 species of Cyanophyta, 19 species of Bacillariophyta, 23 species of Chlorophyta, 14 species of Protozoa, 29 species of Rotifera, 4 species of Cladocera and 6 species of Copepoda. Bimodal blooming of phytoplankton was observed. A large blooming ($1,504\times10^3\;cells/l$ in October) was observed from July to October; a small blooming was present ($236\times10^3\;cells/l$ in February) from January to April. The dominant phytoplankton species include Melosira granulata, Anabaena spiroides, Asterionella gracillima and Microcystis aeruginota, which were classified into three seasonal groups : summer group, winter group and the whole year group. The sumner group includes Melosira granulate and Anabaena spiroides ; the winter group includes Asterionella gracillima and Synedra acus, S. ulna: the whole year group includes Microtystis aeruginosa and Ankistrodesmus falcatus. It is noted that M. granulate tends to aggregate in the bottom layer from January to August. The dominant zooplankters were Thermocpclops taihokuensis, Difflugia corona, Bosmina longirostris, Bosminopsis deitersi, Keratelle quadrata and Asplanchna priodonta. A single peak of zooplankton growth was observed and maximum zooplankton occurrence was present in July. Diurnal vertical migration was revealed by Microcystis aeruginosa, M. incerta, Anabaena spiroides, Melosira granulata, and Bosmina longirostris. Of these, M. granulata descends to the bottom and forms aggregation after sunset. B. longirostris shows fairly typical nocturnal migration. They ascends to the surface after sunset and disperse in the whole water column during night. Foully one species of fish representing 31 genera were collected. Of these 13 species including Pseudoperilnmpus uyekii and Coreoleuciscus splendidus were indigenous species of Korean inland waters. The indicator species of water quality determination include Microcystis aeruginosa, Melosira granulata, Asterionelta gracillima, Brachionus calyciflorus, Filinia longiseta, Conochiloides natans, Asplanchna priodonta, Difflugia corona, Eudorina elegans, Ceratium hirundinella, Bosmina longirostris, Bosminopsis deitersi, Heliodiaptomus kikuchii and Thermocyclops taihokuensis. These species have been known the indicator groups which are commonly found in the eutrophic lakes. Based on these planktonic indicators Lake Ok-Jeong can be classified into an eutrophic lake.