• 대한기계학회논문집B
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• 제39권5호
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• pp.435-441
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• 2015

본 연구에서는 원심압축기 임펠러와 디퓨져 블레이드 형상을 반응표면법과 다목적 유전알고리즘 기법을 사용하여 최적설계 연구를 수행하였다. 임펠러와 디퓨져의 블레이드 선단과 후단의 각도와 두께를 3 구간으로 나누어 설계변수로 적용하였으며 수치해석은 상용코드인 ANSYS CFX 를 사용하였다. 실험계획법 중 많이 사용되는 중심합성계획을 이용하여 총 45 개의 설계점에 대한 값을 계산하였다. 계산된 결과를 바탕으로 반응표면을 생성하였으며 반응표면은 최적형상의 임펠러와 디퓨져를 선정하는데 이용하였다. 최적설계의 전 과정은 ANSYS DX 를 사용하였으며, 최적화의 결과로 원심압축기의 주요 성능변수인 등엔트로피 효율과 압력회복계수가 각각 0.3%, 5% 향상된 임펠러와 디퓨져 블레이드 형상을 제시하였다.

• 한국수소및신에너지학회논문집
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• 제24권1호
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• pp.50-60
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• 2013

High temperature proton exchange membrane fuel cells (PEMFCs) using phosphoric acid (PA) doped polybenzimidazole (PBI) membranes have been concentrated as one of solutions to the limits with traditional low temperature PEMFCs. However, the amount of reported experimental data is not enough to catch the operational characteristics correlated with cell performance and durability. In this study, design of experiments (DOE) based operational optimization method for high temperature PEMFCs has been proposed. Response surface method (RSM) is very useful to effectively analyze target system's characteristics and to optimize operating conditions for a short time. Thus RSM using central composite design (CCD) as one of methodologies for design of experiments (DOE) was adopted. For this work, the statistic models which predict the performance and degradation rate with respect to the operating conditions have been developed. The developed performance and degradation models exhibit a good agreement with experimental data. Compared to the existing arbitrary operation, the expected cell lifetime and average cell performance during whole operation could be improved by optimizing operating conditions. Furthermore, the proposed optimization method could find different new optimal solutions for operating conditions if the target lifetime of the fuel cell system is changed. It is expected that the proposed method is very useful to find optimal operating conditions and enhance performance and durability for many other types of fuel cell systems.

• 한국식품영양학회지
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• 제27권6호
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• pp.1175-1181
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• 2014

This study was conducted to develop for the elderly food that is safe, well-shaped, and easy to chew and swallow, using gelification. Gelatin, which has low adhesiveness, thereby lowering the possibility of being swallowed wrongly into the respiratory track, was used as a viscosity agent for the gelification. Water and gelatin amounts were adjusted to facilitate breaking the food with the tongue. Various foods for the elderly with chewing and swallowing difficulties were used for sensory assessment. Sensory panel consisted of 10 dietitians (10 women) in nursing care facilities. The sensory optimal composite recipes were determined by central composite design (CCD). The sensory measurements were significantly different in appearance (p<0.05), saltiness (p<0.05), sweetness (p<0.01), and overall quality (p<0.05). The optimum formulation of the Korean barbecue beef calculated by numerical and graphical method was 3.71 g of sugar and 19.53 g of soy sauce. Moisture content, hardness and adhesiveness of the Korean barbecue beef were 78.85%, 2.40, -1.87, respectively. The result shows that food for the elderly, which is easy to chew and swallow, using gelification will have sufficient competitiveness in terms of safety, taste, and preference. The development of food for the elderly that take into account the difficulties in chewing and swallowing among the elderly, reflects their preference, and has sufficient nutrients, is important to enable the elderly to enjoy their meals. Also, as the Korean society is getting older, it remains one of the biggest social challenges.

• 한국식품영양학회지
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• 제29권4호
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• pp.480-485
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• 2016

This study was conducted to develop food for the elderly, which is easier to chew and swallow, using gelification. Various foods for the elderly with chewing and swallowing difficulties were used for sensory assessment. The sensory panel consisted of 10 dieticians (10 women) working in nursing care facilities. The sensory optimal composite recipes were determined by the central composite design (CCD). The optimum formulation of hot pepper paste stir fried pork calculated by the numerical and graphical method was 1.48 g of sugar and 18.97 g of hot pepper paste. The optimum formulation of seasoned spinach was 8.0 g of sesame oil and 5.41 g of soy sauce. Moisture content, hardness, and adhesiveness of hot pepper paste stir fried pork was 76.49%, 2.50, and -1.20, respectively. Moisture content, hardness, and adhesiveness of seasoned spinach was 83.48%, 2.27, and -1.17, respectively. This study provides the basic materials for the development of easily chewable and swallowable foods for the elderly, which can reduce the risk of food going down the wrong pipe, and the preference can be improved by eating solid food instead of porridge or liquid food. The development of food for the elderly, which takes the difficulties in chewing and swallowing among the elderly into consideration and reflects their preference and has sufficient amount of nutrients, is important to enable the elderly to enjoy their meals and it is one of the biggest challenges in Korea, as Korean society is aging rapidly.

• 한국재료학회지
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• 제34권7호
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• pp.341-354
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• 2024

Today, the principles of green chemistry are being fundamentally applied in the chemical industry, such as the nitrobenzene industry, which is an essential intermediate for various commercial products. Research on the application of response surface methodology (RSM) to optimize nitrobenzene synthesis was conducted using a sulfated silica (SO₄/SiO₂) catalyst and batch microwave reactor. The nitrobenzene synthesis process was carried out according to RSM using a central composite design (CCD) design for three independent variables, consisting of sulfuric acid concentration on the silica (%), stirring time (min), and reaction temperature (℃), and the response variable of nitrobenzene yield (%). The results showed that a three-factorial design using the response surface method could determine the optimum conditions for obtaining nitrobenzene products in a batch microwave reactor. The optimum condition for a nitrobenzene yield of 63.38 % can be obtained at a sulfuric acid concentration on the silica of 91.20 %, stirring time of 140.45 min, and reaction temperature of 58.14 ℃. From the 20 experiments conducted, the SO₄/SiO₂ catalyst showed a selectivity of 100 %, which means that this solid acid catalyst can potentially work well in converting benzene to nitrobenzene.

• 공업화학
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• 제35권4호
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• pp.316-320
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• 2024

천연물인 황금추출물과 천연유화제인 올리브 유화왁스를 사용하여 제조된 cosmeceuticals의 유화안정성 및 대장균에 대한 항균능력을 최적화하였다. 독립변수로는 황금추출물 첨가량과 올리브 유화왁스의 첨가량으로 설정하였으며, 반응치로는 제조한 cosmeceuticals의 유화안정도지수(ESI)와 대장균 억제직경을 설정하였다. CCD-RSM을 통하여 95% 신뢰구간 내에서 통계학적으로 합리적이며 신뢰성이 우수한 회귀방정식을 얻었다. 다중반응의 최적화를 통하여 유화안정도지수와 대장균 억제직경을 동시에 만족하는 최적 유화조건으로 황금추출물 첨가량은 3.7 wt%, 올리브 유화왁스의 첨가량은 2.7 wt%이었으며, 산출된 유화안정도지수와 대장균 억제직경의 예측값은 각각 97.9%와 9.7 mm로 나타났다. 최적 조건에서 실제 실험을 진행한 결과 유화안정도지수 및 대장균 억제직경은 각각 95.0%와 9.4 mm로 측정되어 다중반응에 의한 예측값과의 평균오차율은 3.2 ± 0.4%로 나타났다.

• Ocean and Polar Research
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• 제39권4호
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• pp.269-277
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• 2017

Medium compositions for the hyperthermophilic archaeon, Thermococcus onnurineus NA1 was statistically optimized to enhance formate-driven hydrogen ($H_2$) production by using response surface methodology. From the Plackett-Burman design-based experiment, it was confirmed that among the minor components of medium such as KCl, $MgSO_4$, $NH_4Cl$, Cystein-HCl, trace elements, Fe-EDTA and $CaCl_2$, the trace elements were screened as the only positively effective components with respect to $H_2$ production. Subsequently, the optimal concentrations of the trace elements and the major components of a medium such as NaCl, yeast extract and sodium formate were determined from the five-level central composite design (CCD)-based experiment. The resulting quadratic model predicted the maximum $H_2$ production of 46.6 mmol/L in serum bottle and it was validated experimentally using the optimal medium initially supplemented with 26.70 g/L of NaCl, 9.81 g/L of sodium formate, 3.50 g/L of yeast extract and 4.59 mL/L of trace elements. From the duplicate batch cultivations in the fermentor using the optimized medium, the a maximum $H_2$ production rate up to 71.8 mmol/L/h could be obtained, which was a 65% enhanced value compared with that obtained using the control medium, showing the high efficiency of the optimized medium.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1252-1260
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• 2020

(R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H₂O₂ addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H₂O₂, identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H₂O₂ 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03-fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.381-384
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• 2002

Response surface methodology (RSM) was successfully applied to optimize for the production of Ganoderma lucidum in batch fermentations using the whey (40,000 mg latose/L) as substrate. This study was performed according to the central composite design (CCD) with respect to pH and temperature, where the designed intervals were 3.3$22.9^{\circ}C$$37.1^{\circ}C$, respectively. A second-order factorial design of the experiments was used to build empirical models providing a quantitative interpretation of the relationships between the two variables. The optimum conditions to maximize the production of G. lucidum were pH 4.2 and $28.3^{\circ}C$. At optimum conditions, the mycelial dry weight (MDW) and residual soluble COD (SCOD) were simultaneously used to evaluate the biokinetic coefficients assocoated with substrate inhibition model by nonlinear least squares method with 95% confidence interval. The. maximum microbial growth rates (${\mu}m$), half saturation coefficient ($K_s$), and the inhibition substrate concentration ($K_{is}$) were determined to be 0.095 l/hr, 128,000 mg SCOD/L and 49,000 mg SCOD/L, respectively. And the microbial yield coefficient (Y), biomass decay rate coefficient ($K_d$), and the maintenance energy coefficient ($m_s$) were determined to be 0.37 mg MDW/mg SCOD, 0.001 1/hr, and 0.0015 1/hr, respectively.

• 한국미생물·생명공학회지
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• 제41권1호
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• pp.52-59
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• 2013

The nutritional requirements for the maximum production of lipopeptides by Bacillus subtilis N7 (B. subtilis N7) were investigated and optimized using response surface methodology (RSM) under shake flask fermentation. A one-factor-at-a-time experimental setup was used to screen carbon and nitrogen sources. A Plackett-Burman design (PBD) was employed to screen the most critical variables for lipopeptides production amongst ten nutritional elements. The central composite experimental design (CCD) was finally adopted to elucidate the composition of the fermentation medium. Statistical analyses (analysis of variance, ANOVA) of the results showed that KCl, $MnSO_4$ and $FeSO_4{\cdot}6H_2O$ were important components and that their interactions were strong. Lipopeptide production was predicted to reach 709.87 mg/L after a 60 h incubation using an optimum fermentation medium composed of glucose 7.5 g/L, peanut oil 1.25 g/L, $MgSO_4$ 0.37 g/L, $KH_2PO_4$ 0.75 g/L, monosodium glutamate 6.75 g/L, yeast extract and $NH_4Cl$ (5:3 w/w) 10 g/L, KCl 0.16 g/L, $FeSO_4{\cdot}6H_2O$ 0.24 mg/L, $MnSO_4$ 0.76 mg/L, and an initial pH of 7.0. Lipopeptide production ($706.57{\pm}3.70$ mg/L) in the optimized medium confirmed the validity of the predicted model.