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Effect of Modified Atmosphere Packaging in Microperforated Film on Maintenance of the Quality of Fresh-Cut Apples (미세 천공 필름에 의한 신선절단 사과의 MA포장 효과)

  • Chung, Hun-Sik;Toivonen, Peter;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.15 no.3
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    • pp.347-351
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    • 2008
  • The effect of microperforated packaging films on fresh-cut apples was studied Apples (Malus domestica Borkh. cv. Red Delicious) were cored and cut, packaged in laser microperforated film or non-microperforated polyolefin film, and stored for 3 weeks at 4C. The flesh firmness of apples packaged in microperforated film during the storage period was significantly higher than that of apples packaged in non-microperforated film, and the level of soluble solids was also higher. The browning index, titratable acidity, pH, acetaldehyde and ethanol levels were not affected by microperforation. These results show that microperforated films could be used for retention of flesh firmness in fresh-cut apples.

Construction of Exchange Table of Earth Quantity for Forest Road Plan (임도설계용(林道設計用) 토량환산표(土量換算表)의 제작(製作))

  • Ma, Sang Kyu
    • Journal of Korean Society of Forest Science
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    • v.76 no.4
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    • pp.348-356
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    • 1987
  • A new simple method to estimate the earth quantity which is basic data for making the plan of mountain forest road is tried to develop as one of ways to reduce time cost of plan making. The zero-line surveying method, the concept of 2/3 road width in cut-away part and of same quantity between cut-away and fill-up earth which are the backgrounds to develop this method is introduced and explaned also it's theoritical propriety. Under the precondition which have to apply above concepts for constructing the mountain forest road the exchange table for estimating the earth quantity is made like table 3 and 4 through being calculated by a trigonometric function. Table 3 can be used to estimate the earth quantity on the cut-away road-width according to slope of terrain and different road-width, and table 4 can be applied to calculate the quantity of cut-away earth when to know the road-width of cut-away. When using these tables the performance of field surveying and office work for road plan will be highly increased.

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Risk Assessment of Cut Slope by Gravity Field Interpretation and Modelling (비탈면 위험도 평가를 위한 중력장 해석 및 모델링)

  • Choi, Sungchan;Kim, Sung-Wook;Choi, Eun-Kyoung;Lee, Yeong-Jae;Jang, Hyun-Ick
    • The Journal of Engineering Geology
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    • v.31 no.4
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    • pp.533-540
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    • 2021
  • Gravity field analysis and density modeling were performed to evaluate the internal state of the rock mass, which is the cause of cut slope collapse. The shape of the weathered zone and the depth of basement could be confirmed from the complete Bouguer anomaly and density model. The basement depth at the center of the cut slope calculated using the Euler deconvolution inverse method is 30 m, which is about 10 m deeper than the surrounding area. In addition, the depth of basement and the thickness of the weathered zone are similar to the boundary between low resistivity and high resistivity in dipole-dipole survey. From the study results, gravity field analysis and density modeling recognizes the internal state of the rock slope and can be used for slope safety analysis, and is particularly suitable as a method to determine the shape of weathered zones in interpreting the safety of cut slopes

Combination of Enrichment and PCR in Rapid Semi-Quantification of Bacillus cereus in Fresh-Cut Vegetables

  • Choi, Yukyung;Lee, Sujung;Yoon, Yohan
    • Journal of Food Hygiene and Safety
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    • v.35 no.4
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    • pp.319-325
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    • 2020
  • Widespread consumption of fresh-cut vegetables without cooking results in ingestion of major foodborne pathogens including Bacillus cereus. In this study, we aimed to develop a method to rapidly detect B. cereus in fresh-cut vegetables by combining commercial PCR analysis with enrichment of the pathogenic levels. A mixture of B. cereus strains (KCTC1013, KCTC1014, KCTC1092, KCTC1094, and KCTC3624) was inoculated on the surface of fresh-cut cabbage lettuce (20 g) and baby leafy vegetables (10 g) to concentration 1, 2, 3, 4, and 5 log CFU/g. Eighty milliliters of TSB with 0.15% polymyxin B was used for cabbage lettuce, and 90 mL of medium was used for baby leafy vegetables and incubated at 42℃ for 0, 2, 3, 4, 5, 6, and 7 h. One milliliter of the enriched media was plated on mannitol-egg yolk-polymyxin agar for quantification, and another 1 mL was used for DNA extraction for PCR analysis. Additionally, the minimum number of sub-samples to be tested from a pack of fresh-cut vegetable samples was determined using 5 sub-samples. The results from this study showed that for detecting B. cereus in fresh-cut cabbage lettuce, 3, 4, 5, 6, and 7 h enrichment were required to at least detect 5, 4, 3, 2, and 1 log CFU/g of B. cereus, respectively. B. cereus in fresh-cut baby leafy vegetables could be detected after 2, 3, 4, 5, and 6 h of enrichment at 5, 4, 3, 2, and 1 log CFU/g, respectively, using a combination of enrichment and PCR analysis. To determine if a pack of fresh-cut vegetable is positive, the minimum number of sub-samples should be 3. These results can be used to develop a rapid detection method to semi-quantify B. cereus in fresh-cut vegetable samples combining enrichment and PCR.

Improving Diagnostic Accuracy for Malignant Nodes and N Staging in Non-Small Cell Lung Cancer Using CT-Corrected FDG-PET (비소세포폐암에서 CT-보정 양전자단층촬영술을 이용한 악성 림프절 평가 및 N 병기 결정 성적 향상)

  • Lee, Eun-Jeong;Choi, Joon-Young;Lee, Kyung-Soo;Chung, Hyun-Woo;Lee, Su-Jin;Cho, Young-Seok;Choi, Yong;Choe, Yearn-Seong;Lee, Kyung-Han;Kwon, O-Jung;Shim, Young-Mog;Kim, Byung-Tae
    • The Korean Journal of Nuclear Medicine
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    • v.39 no.4
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    • pp.231-238
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    • 2005
  • Purpose: We investigated prospectively whether the interpretation considering the patterns of FDG uptake and the findings of unenhanced CT for attenuation correction can improve the diagnostic accuracy for assessing malignant lymph node (LN) and N stage in non-small cell lung cancor (NSCLC) using CT-corrected FDG-PET (PET/CT). Materials & Methods: Subjects were 91 NSCLC patients (M/F 62/29, age: $60{\pm}9$ yr) who underwent PET/CT before in dissection. We evaluated the maximum SUV (maxSUV), patterns of FDG uptake, short axis diameter, and calcification of LN showing abnormally increased FDG uptake. Then we investigated criteria improving the diagnostic accuracy and correlated results with postoperative pathology. In step 1, in was classified as benign or malignant based on maxSUV only. In step 2, LN was regarded as benign if it had lower maxSUV than the cut-off value of step 1 or it had calcification irrespective of its maxSUV. In step 3, LN regarded as malignant in step 2 was classified as benign if they had indiscrete margin of FDG uptake. Results: Among 432 LN groups surgically resected (28 malignant, 404 benign), 71 showed abnormally increased FDG uptake. We determined the cut-off as maxSUV=3.5 using ROC curve analysis. The sensitivity, specificity, and accuracy for assessing malignant LN were 64.3%, 86.9%, 85.4% in step 1, 64.3%, 95.0%, 93.1% in step 2, and 57.1%, 98.0%, 95.4% in step3, respectively. The accuracy for assessing N stage was 64.8% in step 1, 80.2% in step 2, and 85.7% in step 3. Conclusion: interpreting PET/CT, consideration of calcification and shape of the FDG uptake margin along with maxSUV can improve the diagnostic accuracy for assessing malignant involvement and N stage of hilar and mediastinal LNs in NSCLC.

Effects of Felling of Damaged Tree of Pine Wilt Disease on Soil Respiration in Pinus densiflora Stands (소나무재선충병 피해목 벌채가 토양호흡에 미치는 영향)

  • Cho, Min-Gi;Jeon, Kwon-Seok;Park, Jun-Ho;Kim, Jong-Kab;Moon, Hyun-Shik
    • Journal of agriculture & life science
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    • v.43 no.2
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    • pp.9-15
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    • 2009
  • The objectives of this study was to obtain the basic information for reasonable management of soil ecosystem damaged by pine wilt disease. Soil temperature and moisture content were $15.3{^{\circ}C}$ and 11.5% at whole-cut site, $14.4{^{\circ}C}$ and 13.5% at partial-cut site, and $13.7{^{\circ}C}$ and 14.8% at control site, respectively. The content of soil organic matter throughout the study period ranged from 2.11 to 2.64% at whole-cut site, 2.26 to 3.33% at partial-cut site, and 2.27 to 3.10% at control. Soil respiration rates showed seasonal fluctuations increasing in summer, which showed positive correlations between soil respiration and soil temperature. Average soil respiration were 0.24, 0.36 and $0.32gCO_{2}/m^{2}/hr$ at whole-cut site, partial-cut site, and control, respectively. $Q_{10}$ values ranged from 2.39 to 2.68 at Pinus densiflora stands damaged by pine wilt disease. Annual soil respiration rate at whole-cut site, partial-cut site and control were 8.1, 15.6 and $14.6tCO_{2}/ha/yr$, respectively.

Changes of Microorganisms During Fresh-Cut Cabbage Processing: Focusing on the Changes of Air-Borne Microorganisms (신선편이 양배추 제조공정 단계별 미생물 변화: 공기 중 미생물 변화를 중심으로)

  • Seo, Jung-Eun;Lee, Jong-Kung;Oh, Se-Wook;Koo, Min-Seon;Kim, Young-Ho;Kim, Yun-Ji
    • Journal of Food Hygiene and Safety
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    • v.22 no.4
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    • pp.288-293
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    • 2007
  • To evaluate effects of airborne microorganisms in fresh cut processing plant, microorganisms in air, equipments, raw material, water and final product were isolated and identified using Vitek (R)2 compact system. Airborne microorganisms were isolated from 1000L air using air sampler for floating microorganisms and plate count agar for falling microorganisms. And contaminated microorganisms of equipment, water, and product were isolated from plate count agar plate. Total plate counts for floating and falling, raw material, equipments and final product were $10^2-10^3CFU/m^3,{\sim}10^1CFU/plate,\;10^3CFU/g,{\sim}10^4CFU/cm^2\;and\;10^4CFU/g$, respectively. From the result of isolated microorganism identification from raw material to final product, airborne microorganisms could affect the flora of final product.

Electrochemical Properties of Individual Carbon Nanotube Fabricated by Reactive Ion Etching (반응성 이온 식각법에 의해 제작된 탄소나노튜브 전극의 전기화학적 특성)

  • Hwang, Sook-Hyun;Choi, Hyon-Kwang;Kim, Sang-Hyo;Han, Young-Moon;Jeon, Min-Hyon
    • Korean Journal of Materials Research
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    • v.21 no.2
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    • pp.89-94
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    • 2011
  • In this work, fabrication and electrochemical analysis of an individual multi-walled carbon nanotube (MWNT) electrode are carried out to confirm the applicability of electrochemical sensing. The reactive ion etching (RIE) process is performed to obtain sensitive MWNT electrodes. In order to characterize the electrochemical properties, an individual MWNT is cut by RIE under oxygen atmosphere into two segments with a small gap: one segment is applied to the working electrode and the other is used as a counter electrode. Electrical contacts are provided by nanolithography to the two MWNT electrodes. Dopamine is specially selected as an analytical molecule for electrochemical detection using the MWNT electrode. Using a quasi-Ag/AgCl reference electrode, which was fabricated by us, the nanoelectrodes are subjected to cyclic voltammetry inside a $2{\mu}L$ droplet of dopamine solution. In the experiment, RIE power is found to be a more effective parameter to cut an individual MWNT and to generate "broken" open state, which shows good electrochemical performance, at the end of the MWNT segments. It is found that the pico-molar level concentration of analytical molecules can be determined by an MWNT electrode. We believe that the MWNT electrode fabricated and treated by RIE has the potential for use in high-sensitivity electrochemical measurement and that the proposed scheme can contribute to device miniaturization.

Predictive Values of Magnetic Resonance Imaging Features for Tracheostomy in Traumatic Cervical Spinal Cord Injury

  • Jeong, Tae Seok;Lee, Sang Gu;Kim, Woo Kyung;Ahn, Yong;Son, Seong
    • Journal of Korean Neurosurgical Society
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    • v.61 no.5
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    • pp.582-591
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    • 2018
  • Objective : To evaluate the magnetic resonance (MR) imaging features that have a statistically significant association with the need for a tracheostomy in patients with cervical spinal cord injury (SCI) during the acute stage of injury. Methods : This study retrospectively reviewed the clinical data of 130 patients with cervical SCI. We analyzed the factors believed to increase the risk of requiring a tracheostomy, including the severity of SCI, the level of injury as determined by radiological assessment, three quantitative MR imaging parameters, and eleven qualitative MR imaging parameters. Results : Significant differences between the non-tracheostomy and tracheostomy groups were determined by the following five factors on multivariate analysis : complete SCI (p=0.007), the radiological level of C5 and above (p=0.038), maximum canal compromise (MCC) (p=0.010), lesion length (p=0.022), and osteophyte formation (p=0.015). For the MCC, the cut-off value was 46%, and the risk of requiring a tracheostomy was three times higher at an interval between 50-60% and ten times higher between 60-70%. For lesion length, the cut-off value was 20 mm, and the risk of requiring a tracheostomy was two times higher at an interval between 20-30 mm and fourteen times higher between 40-50 mm. Conclusion : The American Spinal Injury Association grade A, a radiological injury level of C5 and above, an MCC ${\geq}50%$, a lesion length ${\geq}20mm$, and osteophyte formation at the level of injury were considered to be predictive values for requiring tracheostomy intervention in patients with cervical SCI.

Chemotherapy for Malignant Gliomas Based on Histoculture Drug Response Assay : A Pilot Study

  • Gwak, Ho-Shin;Park, Hyeon-Jin;Yoo, Heon;Youn, Sang-Min;Rhee, Chang-Hun;Lee, Seung-Hoon
    • Journal of Korean Neurosurgical Society
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    • v.50 no.5
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    • pp.426-433
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    • 2011
  • Objective : The Histoculture Drug Response Assay (HDRA), which measures chemosensitivity using minced tumor tissue on drug-soaked gelfoam, has been expected to overcome the limitations of in vitro chemosensitivity test in part. We analyzed interim results of HDRA in malignant gliomas to see if the test can deserve further clinical trials. Methods : Thirty-three patients with malignant gliomas were operated and their tumor samples were examined for the chemosensitivity to 10 chosen drugs by HDRA. The most sensitive chemotherapy regimen among those pre-established was chosen based on the number of sensitive drugs or total inhibition rate (IR) of the regimen. The response was evaluated by 3 month magnetic resonance image. Results : Among 13 patients who underwent total resection of the tumor, 12 showed no evidence of disease and one patient revealed progression. The response rate in 20 patients with residual tumors was 55% (3 complete and 8 partial responses). HDRA sensitivity at the cut-off value of more than one sensitive drug in the applied regimen showed a sensitivity of 100%, specificity of 60% and predictability of 70%. Another cut-off value of >80% of total IR revealed a sensitivity of 100%, specificity of 69%, and predictability of 80%. For 12 newly diagnosed glioblastoma patients, median progression-free survival of the HDRA sensitive group was 21 months, while that of the non-sensitive group was 6 months ($p$=0.07). Conclusion : HDRA for malignant glioma was inferred as a feasible method to predict the chemotherapy response. We are encouraged to launch phase 2 clinical trial with chemosensitivity on HDRA.