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Modified QuEChERS Multi-Residue Analysis Method for 61 pesticides in Fruits using with HPLC and GC-ECD/NPD (HPLC 및 GC-ECD/NPD를 이용한 과일 중 61종 농약의 QuEChERS 전처리 다성분 분석법 개발)

  • Lee, Ju-Young;Hong, Su-Myeong;Kim, Taek-Kyum;Min, Zaw Win;Kim, Yang-Hyeon;Song, Kyung-Ae;Kwon, Hye-Yong;Lee, Hee-Dong;Im, Geon-Jae;Kim, Doo-Ho;Kim, Jang-Eok
    • The Korean Journal of Pesticide Science
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    • v.16 no.3
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    • pp.242-256
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    • 2012
  • QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method has been a lot of research for pesticide analysis, because it is very simple and fast. However, this method requires high sensitivity instrument such as LC-MS/MS because of the use of small sample volume and many impurities compared to the conventional method. So, QuEChERS method needs to be modified for using with HPLC and GC-ECD/NPD. The aim of this work was to study the application of the QuEChERS method as well as its modification for the extraction and preconcentration of 5 groups of 61 pesticides from 4 fruits prior to their determination by HPLC-PDA, GC-ECD/NPD, and LC-MS/MS. The method was validated using spiking levels at 0.1 mg/kg (or 0.01 mg/kg) in apple, grapes, pear and persimmon. The average recovery by QuEChERS AOAC Official 2007. 01 version using the LC-MS/MS varied from 71.1127.4% for 61 pesticides. The average recovery rates using modified QuEChERS varied from 70.9~126% for 61 pesticides by HPLC-PDA and GC-ECD/NPD. The results satisfied the criteria of multiple pesticide residue analysis, setting 70~130% for recovery rates and below 30% for CV.

Fish Fauna and Ecological Characteristics of Dark Chub (Zacco temminckii) Population in the Mid-Upper Region of Gam Stream (감천 중 ${\cdot}$ 상류역의 어류상과 갈겨니 (Zacco temminckii) 개체군의 생태학적 특성)

  • Seo, Jin-Won
    • Korean Journal of Ecology and Environment
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    • v.38 no.2 s.112
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    • pp.196-206
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    • 2005
  • The fish community in the mid-upper region of Gam Stream was examined seasonally from 2001 to 2003 in order to perform an environmental impact assessment prior to a construction of Gamcheon Multipurpose Dam. Additional investigation was conducted in August 2004 to confirm the fish fauna reported and to examine the ecological characteristics of Zacco temminckii population. The total number of fish caught from the study sites was 1,081 fish representing 5 families 14 species. There were 6 Korean endemic species including Coreoleuciscus splendidus, Squalidus gracilis majimae, Microphysogobio yaluensis, Liobagrus mediadiposalis, Coreoperca herzi and Odontobutis platycephala, but no endangered or vulnerable species were found. Length-weight relation, condition factor (K) and relative condition factor (Kn) of Zacco temminckii were compared by the study sites and stream. The equations based on length-weight relation in Buhang and Gam Streams were TW\;=\;0.000004TL^{3.2357}$ and TW\;=\;0.000002TL^{3.3566}$, respectively indicating the fish in Gam Stream became more rotund as length increases. The condition factor (K) and relative condition factor (Kn) against total length of Zacco temminckii at two streams indicated that the fish (>70 mm) in Cam Stream (mean K and Kn= 1.116, 1.21 respectively) had better nutritional condition than those in Buhang Stream (mean K and Ln = 1.046, 1.14 respectively). The results were corresponded with natural disturbances such as drought and intensive rainfall from 2001 to 2003 followed by human activities such as stream repair works. Therefore, it is considered to perform environmental impact assessment with not only confirmation of fish composition but also examination of ecological characteristics in population- level.

Variations in Ammonium Removal Rate with Tidal State in the Macrotidal Han River Estuary: Potential Role of Nitrification (한강기수역에서의 암모늄 제거율 변화 및 질산화의 잠재적 역할)

  • Hyun, Jung-Ho;Chung, Kyung-Ho;Park, Yong-Chul;Choi, Joong-Ki
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.1
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    • pp.33-39
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    • 1999
  • In order to understand the importance of tidal action and $NH_4{^+}$ -nitrification in the removal of dissolved oxygen (DO) and $NH_4{^+}$, concentrations of DO, $NH_4{^+}$, $NO_2{^-}$ and $NO_3{^-}$ were measured with time for water samples collected at different tidal state in the eutrophic macrotidal Han River estuary. Field measurements indicated that most environmental parameters, except for the water temperature and DO concentration, were tightly controlled by the eutrophic freshwater runoff and large-scale tidal action. Dark incubation of the water sample at $25^{\circ}C$ showed that the removal rates of DO and $NH_4{^+}$ in high tide sample were 2.76 ${\mu}M\;O_2\;d^{-1}$ and 1.76 ${\mu}M\;N\;d^{-1}$ respectively, and increased to 5.66 ${\mu}M\;O_2\;d^{-1}$ and 3.36 ${\mu}M\;N\;d^{-1}$ respectively, in low tide sample. These changes indicated that microbial degradation and uptake of organic matter and inorganic nutrients were more active during low tide. $NH_4{^+}$-nitrification responsible for total DO removal in low tide (23.81%) and $NH_4{^+}$ turnover rates due to $NH_4{^+}$-nitrification in low tide (0.18 $d^{-1}$) were approximately 3.7 times and 3 times, respectively, higher than those in high tide. These results indicated that $NH_4{^+}$ -nitrifying bacteria introduced into the Han River estuary during low tide played a significant role in the removal of DO and $NH_4{^+}$. The decreasing removal rates in DO and $NH_4{^+}$ with the increasing tidal level seemed to be associated with the salinity impact on the halophobic freshwater $NH_4{^+}$-nitrifying bacteria. The results implied that anthropogenic $NH_4{^+}$ sources should be treated prior to the freshwater runoff into the estuary for the effective control of $NH_4{^+}$ in the Han River estuary. These results also suggest that parallel ecological studies on the chemoautotrophic nitrifying bacteria are essential for the elucidation of nitrogen cycles in the eutrophic Han River estuary.

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Change of Carbon Fixation and Economic Assessment according to the Implementation of the Sunset Provision (도시공원 일몰제에 의한 탄소고정량과 경제성 분석에 대한 연구)

  • Choi, Jiyoung;Lee, Sangdon
    • Ecology and Resilient Infrastructure
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    • v.7 no.2
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    • pp.126-133
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    • 2020
  • In accordance with the implementation of the sunset provision to cancel the designations of urban park sites that remained unexecuted for a prolonged period until 2020, the park sites in the city center, which account for 90% of the long-term unexecuted urban facilities subjected to the provision, are currently on the verge of development. The total area of the 204 park sites that will disappear in Seoul as a result of this provision is 95 ㎢; moreover, 116 of these are privately-owned. It is expected that the possible changes in the use of these park sites could result in reckless development and reduction of green space, which would ultimately affect the ecosystem. This study applied the InVEST model to calculate the changes in the fixed carbon amount before and after the implementation of the sunset provision to estimate the economic value of these changes. The study focused on Jongno-gu in Seoul because it has the most unexecuted park sites subjected to the lifting of the designation. The research findings show that the fixed carbon amount provided by the unexecuted park sites in Jongno-gu was 374,448 mg, prior to the implementation of the sunset provision; however, the amount was estimated to decrease by 18% to 305,564 mg after its execution. When calculated in terms of average value of the real carbon price, this translated into a loss of approximately 700 million won. In addition, considering the social costs including both climate change and the impact on the ecosystem, an economic loss of approximately 98 billion won was projected. This study is meaningful because its predictions are based on the estimation of fixed carbon amount according to the implementation of the sunset provision in Jongno-gu and scientifically calculates the value of ecological services provided by the parks in the city. This study can serve not only as a basis during the decision-making process for policies related to ecosystem conservation and development, but also as an evidentiary material for the compensation of privately-owned land that is designated as urban park sites and was unexecuted for a prolonged period.

Chemical Composition and Fermentation Characteristics of the Corn Silage During Feedout at Yonchon of Gyeonggi-do (연천지역에 있어서 옥수수 사일리지의 개봉 후 경과기간에 따른 시료성분 및 발효품질)

  • Sung Kyung Il;Kim Gon Sik;Lee June Woo;Kim Byung Wan;Kim Sang Rok
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.1
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    • pp.43-50
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    • 2005
  • The purpose of this study is to discuss the ways to consistently feed high quality corn silage(CS). This study evaluated the effect of the corn silage, after a certain time has elapsed, on the chemical composition and fermentation characteristics after feedout during the winter feeding period of the CS. Six samples of CS from four dairy farms(E1, E2, E3, and L1 ) were taken in order to feed the milking cows over a winter feeding Period from November of 2002 until February of the following year, 2003(six samples were taken at the fellowing dates in the following order: sample one was taken on the 23rd Nov. 2002, sample two on the 5th of Dec. 2002, 3rd sample on the 23rd of Dec. 2002, 4th sample on the 7th of Jan. 2003, 5th sample on the 22nd of Jan. 2003, and the 6th sampling was carried out on the 6th of Feb. 2003) at the three sampling sites after the opening of the trench silos at intervals of 15 days. In the dry matter contents of CS, there wasn't any specific tendency according to the elapsed time in the range of 21.3~$27.3\%$ at all low dairy farm(E1, E2, E3, and L1). And the average dry matter contents were 24.1, 25.9, 23.6, and $20.4\%$. Considering the Proper amount of the dry matter of CS during the ripen yellow stage, the appropriate moisture content was $33\%$ (NRC, 1989), and these dry matter contents were all low. A consistent tendency was not found in the contents of CS. The average of CP contents were 10.2, 8.0, 8.5, and $9.8\%$ at the E1, E2, E3, and L1 farms, and there were significant differences. The TDN contents of CS were not different among forms according to the time elapsed. The pH, according to the time elapsed after opening of the CS, there were no differences at each of E1, E2, E3, md L1 farms. Average pH were 3.5, 3.9, 3.6, and 4.1, md all of them were in normal range. In the lactic acid contents of CS, a consistent tendency was not found among four farms. But according to the time elapsed. there was a goat difference from 1.13~$7.8\%$ The acetate, propionate, and butyrate contents of CS were very low. In this study, there was no significant difference in the CS's chemical composition and fermentation characteristics according to the elapsed time at all four dairy farms. Considering the proper dry matter contents of CS during the ripen yellow stage, the appropriate dry matter content was $33\%$, and dry matter contents of few farms were all low. To enhance the quality of corn silage should be ensiled com at proper dry matter content range from 28 to $35\%$ Therefore, content of the corn plant should be always be closely monitored prior to beginning harvest.

A Study of Quality Control of Nuclear Medicine Counting System and Gamma Camera (핵의학 계측기기 및 감마카메라의 정도관리 연구)

  • 손혜경;김희중;정해조;정하규;이종두;유형식
    • Progress in Medical Physics
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    • v.12 no.2
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    • pp.103-112
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    • 2001
  • Purpose: The purpose of this study was to investigate the current status of performing nuclear medicine quality control in korea and to test selected protocols of quality control of nuclear medicine counting system and gamma camera. Materials and Methods: Fifty three hospitals were included to investigate the current status of nuclear medicine quality control in korea. The precision of dose calibrator and thyroid uptake system was measured with Tc-99m 35.52 MBq for 2 minuets and Tc-99m 5.14 MBq for 10 sec every one minute, respectively. The sensitivity of CeraSPECT$^{TM}$ with low energy high resolution parallel hole collimator was measured using two cylindrical phantoms with 15 cm in diameter and 12 cm and 30 cm in heights containing Tc-99m. The correction factor for sensitivity of CeraSPECT$^{TM}$ was calculated using phantom data. The system planar sensitivity, uniformity, count rate and spatial resolution were measured for Varicam gamma camera with low energy high resolution parallel hole collimator using 140 keV centered 20% energy window, 256$\times$256 or 512$\times$512 matrix sizes. Results: The quality control of dose calibrator and well counter were showed poor performance status. On the other hand, The quality control of gamma camera and other systems were showed relatively good performance status. The results of precision of dose calibrator and thyroid uptake system was $\pm$1.4%(<$\pm$5%) and chi^2=29.7(>16.92), respectively. It showed that the sensitivity of CeraSPECT$^{TM}$ was higher in center slices compared with the edge slices. After correction of nonuniform sensitivities for patient data, it showed better results compare with prior to correction. System planar sensitivity of Varicam gamma camera was 4.39 CPM/MBq. The observed count rate at 20% loss was 102,407 counts/sec (head 1), 113,427 counts/sec (head 2), when input count rate was 81,926 counts/sec (head 1), 90,741 counts/sec (head 2). The spatial resolution without scatter medium were 8.16 mm of FWHM and 14.85 mm of FWTM. The spatial resolution with scatter medium were 8.87 mm of FWHM and 18.87 mm of FWTM. Conclusion: It is necessary to understand the importance of quality control and to perform quality control of nuclear medicine devices.vices.

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Comparison of Ovariectomy and Ovariohysterectomy in Terms of Postoperative Pain Behavior and Surgical Stress in Dogs (개에서 난소자궁절제술과 난소절제술 후 나타나는 통증 행동과 스트레스 반응의 비교)

  • Lee, Scott-S.;Lee, Seung-Yong;Park, Se-Jin;Kim, Young-Ki;Seok, Seong-Hoon;Hwang, Jae-Min;Lee, Hee-Chun;Yeon, Seong-Chan
    • Journal of Veterinary Clinics
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    • v.30 no.3
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    • pp.166-171
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    • 2013
  • This study was conducted to evaluate the clinical relevance and effectiveness of ovariectomy (OVE) and ovariohysterectomy (OVH) in terms of postoperative pain behavior and surgical stress. Thirteen healthy intact mongrel purpose-bred female dogs were used in this study. OVE was performed in six dogs whereas OVH was performed in seven dogs. Prior to, 1, 2, 4, 6, 12, and 24 h after surgery, pain assessment was performed by using the Short form of Composite Measure Pain Scale (CMPS-SF) and blood analysis for the determination of glucose, creatine kinase (CK), and cortisol were done. Also, surgical time, duration of anesthesia, and incision length were compared between two groups. As a result, OVH group as opposed to OVE group showed significantly longer in surgical time, duration of anesthesia, and incision length. Also, based on the two-way ANOVA test, the CMPS-SF had significant differences (p < 0.05) between two groups, with the OVE dogs having lower values at 1, 2, 4, and 6 h postoperatively. In addition, in terms of CK, the value at 1, 2, 4, 6, and 12 h were significantly (p < 0.05) increased from the baseline value for the OVH group. However, as for CK in OVE group, the values at 4, 6, and 12 h were significantly increased from the baseline value. As for blood glucose, a significant (p < 0.05) increase from the baseline was shown at 1 h postoperatively in OVH group and no significant increase was shown in OVE group. In terms of serum cortisol level, the values at the 1 and 2 h were significantly (p < 0.05) increased from the baseline value for both groups. In conclusion, our study has shown that OVE can be considered as a superior choice in terms of sterilization technique in female dogs, considering shorter incision length, surgical time, duration of anesthesia along with lower pain and surgical stress response than OVH.

Buyers' Trust in a Brand and Brand Loyalty in the business-to-business (산업재 시장에서 브랜드 신뢰와 브랜드 충성도에 관한 연구)

  • Han, Sang-Rin;Sung, Hyung-Suk
    • Proceedings of the Korean DIstribution Association Conference
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    • 2005.11a
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    • pp.29-51
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    • 2005
  • Brands are important in the consumer market. They are the interface between consumers and the company, consumers may develop loyalty to brands. also, The late development of industrial marketing explains the near absence of research on Brand Equity in business to business. With recent change, industrial companies have shifted from a production focus to a customer focus. industrial brand is fast developing. The basic purpose of this study is to investigate industrial brand trust and loyalty affecting the Result of business relationship between industrial buyers and suppliers. Factors hypothesized to influence trust in a brand include a number of brand characteristics, company characteristics and consumer-brand characteristics. This research presented a comprehensive constructive model consisting of components of industrial brand trust and loyalty, and then propose the research model base on prior researches and studies about relationships among components of industrial brand loyalty. Data were gathered from respondents who work in industrial buying center. For this study, Data were analyzed by SPSS 10.0 and AMOS 4.0. The results of this research analysis were as fallow. Industrial brand trust and loyalty were positively related with a number of industrial brand characteristics, supplier characteristics and buyer-brand characteristics. relationship commitment. This research newly proposed the concept of 'industrial brand trust and loyalty affecting the Result of business relationship between industrial buyers and suppliers'

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The Relative Height Error Analysis of Digital Elevation Model on South Korea to Determine the TargetVertical Accuracy of CAS500-4 (농림위성의 목표 수직기하 정확도 결정을 위한 남한 지역 수치표고모델 상대 오차 분석)

  • Baek, Won-Kyung;Yu, Jin-Woo;Yoon, Young-Woong;Jung, Hyung-Sup;Lim, Joongbin
    • Korean Journal of Remote Sensing
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    • v.37 no.5_1
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    • pp.1043-1059
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    • 2021
  • Forest and agricultural land are very important factors in the environmental ecosystem and securing food resources. Forest and agricultural land should be monitored regularly. CAS500-4 data are expected to be effectively used as a supplement of monitoring forest and agricultural land. Prior to the launch of the CAS500-4, the relative canopy height error analysis of the digital elevation model on South Korea was performed to determine the vertical target accuracy. Especially, by considering area of interest of the CAS500-4 (mountainous or agricultural area), it is conducted that vertical error analysis according to the slope and canopy. For Gongju, Jeju, and Samcheok, the average root mean squared differences were calculated compared to the drone LiDAR digitalsurface models, which were filmed in autumn and winter and the 5 m digital elevation model from the National Geographic Information Institute. As a result, the Shuttle radar topography mission digital elevation model showed a root mean squared differences of about 8.35, 8.19, and 7.49 m, respectively, while the Copernicus digital elevation model showed a root mean squared differences of about 5.65, 6.73, and 7.39 m, respectively. In addition, the root mean squared difference of shuttle radar topography mission digital elevation model and the Copernicus digital elevation model according to the slope angle were estimated on South Korea compared to the 5 m digital elevation model from the National Geographic Information Institute. At the slope angle of between 0° to 5°, root mean squared differences of the Shuttle radar topography mission digital elevation model and the Copernicus digital elevation model showed 3.62 and 2.52 m, respectively. On the other hands root mean squared differences of the Shuttle radar topography mission digital elevation model and the Copernicus digital elevation model respectively showed about 10.16 and 11.62 m at the slope angle of 35° or higher.

The effect of Big-data investment on the Market value of Firm (기업의 빅데이터 투자가 기업가치에 미치는 영향 연구)

  • Kwon, Young jin;Jung, Woo-Jin
    • Journal of Intelligence and Information Systems
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    • v.25 no.2
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    • pp.99-122
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    • 2019
  • According to the recent IDC (International Data Corporation) report, as from 2025, the total volume of data is estimated to reach ten times higher than that of 2016, corresponding to 163 zettabytes. then the main body of generating information is moving more toward corporations than consumers. So-called "the wave of Big-data" is arriving, and the following aftermath affects entire industries and firms, respectively and collectively. Therefore, effective management of vast amounts of data is more important than ever in terms of the firm. However, there have been no previous studies that measure the effects of big data investment, even though there are number of previous studies that quantitatively the effects of IT investment. Therefore, we quantitatively analyze the Big-data investment effects, which assists firm's investment decision making. This study applied the Event Study Methodology, which is based on the efficient market hypothesis as the theoretical basis, to measure the effect of the big data investment of firms on the response of market investors. In addition, five sub-variables were set to analyze this effect in more depth: the contents are firm size classification, industry classification (finance and ICT), investment completion classification, and vendor existence classification. To measure the impact of Big data investment announcements, Data from 91 announcements from 2010 to 2017 were used as data, and the effect of investment was more empirically observed by observing changes in corporate value immediately after the disclosure. This study collected data on Big Data Investment related to Naver 's' News' category, the largest portal site in Korea. In addition, when selecting the target companies, we extracted the disclosures of listed companies in the KOSPI and KOSDAQ market. During the collection process, the search keywords were searched through the keywords 'Big data construction', 'Big data introduction', 'Big data investment', 'Big data order', and 'Big data development'. The results of the empirically proved analysis are as follows. First, we found that the market value of 91 publicly listed firms, who announced Big-data investment, increased by 0.92%. In particular, we can see that the market value of finance firms, non-ICT firms, small-cap firms are significantly increased. This result can be interpreted as the market investors perceive positively the big data investment of the enterprise, allowing market investors to better understand the company's big data investment. Second, statistical demonstration that the market value of financial firms and non - ICT firms increases after Big data investment announcement is proved statistically. Third, this study measured the effect of big data investment by dividing by company size and classified it into the top 30% and the bottom 30% of company size standard (market capitalization) without measuring the median value. To maximize the difference. The analysis showed that the investment effect of small sample companies was greater, and the difference between the two groups was also clear. Fourth, one of the most significant features of this study is that the Big Data Investment announcements are classified and structured according to vendor status. We have shown that the investment effect of a group with vendor involvement (with or without a vendor) is very large, indicating that market investors are very positive about the involvement of big data specialist vendors. Lastly but not least, it is also interesting that market investors are evaluating investment more positively at the time of the Big data Investment announcement, which is scheduled to be built rather than completed. Applying this to the industry, it would be effective for a company to make a disclosure when it decided to invest in big data in terms of increasing the market value. Our study has an academic implication, as prior research looked for the impact of Big-data investment has been nonexistent. This study also has a practical implication in that it can be a practical reference material for business decision makers considering big data investment.