• Clinical and Experimental Reproductive Medicine
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• v.39 no.2
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• pp.58-67
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• 2012

Objective: Previously, we identified that transketolase (Tkt), an important enzyme in the pentose phosphate pathway, is highly expressed at 2 hours of spontaneous maturation in oocytes. Therefore, this study was performed to determine the function of Tkt in meiotic cell cycle regulation, especially at the point of germinal vesicle breakdown (GVBD). Methods: We evaluated the loss-of-function of Tkt by microinjecting Tkt double-stranded RNAs (dsRNAs) into germinal vesicle-stage oocytes, and the oocytes were cultured in vitro to evaluate phenotypic changes during oocyte maturation. In addition to maturation rates, meiotic spindle and chromosome rearrangements, and changes in expression of other enzymes in the pentose phosphate pathway were determined after Tkt RNA interference (RNAi). Results: Despite the complete and specific knockdown of Tkt expression, GVBD occurred and meiosis was arrested at the metaphase I (MI) stage. The arrested oocytes exhibited spindle loss, chromosomal aggregation, and declined maturation promoting factor and mitogen-activated protein kinase activities. The modified expression of two enzymes in the pentose phosphate pathway, Prps1 and Rbks, after Tkt RNAi and decreased maturation rates were amended when ribose-5-phosphate was supplemented in the culture medium, suggesting that the Tkt and pentose phosphate pathway are important for the maturation process. Conclusion: We concluded that Tkt and its associated pentose phosphate pathway play an important role in the MI-MII transition of the oocytes' meiotic cell cycle, but not in the process of GVBD.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.35 no.1
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• pp.1-6
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• 2009

DNA double-strand breaks (DSBs) occur commonly in the all living and in cycling cells. They constitute one of the most severe form of DNA damage, because they affect both strand of DNA. DSBs result in cell death or a genetic alterations including deletion, loss of heterozygosity, translocation, and chromosome loss. DSBs arise from endogenous sources like metabolic products and reactive oxygen, and also exogenous factors like ionizing radiation. Defective DNA DSBs can lead to toxicity and large scale sequence rearrangement that can cause cancer and promote premature aging. There are two major pathways for their repair: homologous recombination(HR) and non-homologous end-joining(NHEJ). The HR pathway is a known "error-free" repair mechanism, in which a homologous sister chromatid serves as a template. NHEJ, on the other hand, is a "error-prone" pathway, in which the two termini of the broken DNA molecule are used to form compatible ends that are directly ligated. This review aims to provide a fundamental understanding of how HR and NHEJ pathways operate, cause genome instability, and what kind of genes during the pathways are associated with head and neck cancer.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.11
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• pp.2512-2520
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• 2013

In this paper, the T-SRR (Triple Split Ring Resonator) using MNG (mu-Negative) meta-material adapted in a low-loss bandpass filter with 3-stages is suggested. The size of the T-SRR in the proposed bandpass filter with low dielectric constant PCB can be easily controlled. And the ${\lambda}/4$ transmission line theory is applied. The proposed T-SRR and filter have the center frequency of 10 GHz with QL value of 184 for military-satellite communication system in I band. The experimental results of the filter show that the insertion and return losses are 1.44 dB and 17.3 dB with bandwidth of 10 %, respectively. The proposed filter will be redesigned by IPD material etc. should be placed here. These instructions give you guidelines for preparing papers for JICCE.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.23 no.5
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• pp.388-396
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• 2010

In this study, we propose characteristics improvement methods according to via hole plating method for FBAR Duplexer with bandwidth($T_x4: 1850 MHz ~ 1910 MHz, $R_x$:1930 MHz ~ 1990 MHz) which is used for wireless systems. Also, we designed and fabricated $3.8{\times}3.8{\times}1.8mm$ size microminiature FBAR Duplexer based on this proposal. First of all, in this study, we fabricated pentagon shape resonators by different size to make filter combination, and their quality factor(Q) are 687 with 6.6% of ${k_{eff}}^2$. Using this resonators, we designed $3{\times}2$ Type $T_x$ filter and $3{\times}4$ Type $R_x$ filter. The transmission line, which works as phase shifter, is designed with 210 ${\mu}m$ in width and 18 mm in length Stripline type. Inductor, which is used for matching component, is designed with width of 75 ${\mu}m$, a technically achievable minimum width. And adopted plating method of filling via hole with conductive epoxy for improved grounding and thermal conductivity. Using these configuration with all of the matching component values, we found Duplexer characteristics of -1.57 dB ~ -1.73 dB in insertion loss, -56 dB in attenuation at 1850 MHz ~ 1910 MHz of $T_x$ band. Also, found -2.71 dB ~ -3.23 dB in insertion loss, -58 dB in attenuation at 1930 MHz ~ 1990 MHz of $R_x$ band.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.2
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• pp.208-216
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• 2014

In case of any coastal ocean near the mouth of huge rivers, low salinity water can be formed due to its large amount of freshwater discharge. For the acoustic analysis on the low salinity environment, some oceanographic data of the East China Sea and the Atlantic Ocean were collected through KODC (Korea Oceanographic Data Center) and NODC (National Oceanographic Data Center) online service. In this paper, the T-S gradient diagram is introduced to show a relation between the gradients of temperature and salinity in view of acoustic surface channel formation. Existence of haline channel, quantitative contribution of gradients of salinity and temperature, effectiveness of the channel formation can be known by the T-S gradient diagram. After applying the collected data into the diagram, tropical regions of the Atlantic Ocean show strong haline channel due to its nearly invariant temperature and drastic change of salinity with depth. The averaged transmission loss in the channel is about 5.7 ~ 7.5 dB less than that out of the channel by the results of acoustic propagation model (RAM: Range independent Acoustic Model). On the other hand, the East China Sea and temperate region of the Atlantic ocean have weaker haline channel with less difference of the averaged transmission loss between in and out of the channel as 3.2 ~ 6.0 dB. Although data samples used in this study have limitation to represent the general physical structures of the three ocean regions, the T-S gradient diagram is shown to be useful and acoustic field affected by low salinity environment is investigated in this study.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.9
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• pp.953-960
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• 2012

An AMTEC (alkali metal thermal electric converter) is a device that is used for the direct conversion of heat to electricity. Sodium is used as the working fluid, and its circulation is driven by a capillary wick. The wicks used for circulation include an evaporator wick, artery wick, and condenser wick, and each wick has a pressure drop because of the circulation of liquid and vapor. For the circulation of sodium, the capillary pressure of the evaporator wick must be greater than the total pressure drop in the wicks. In this study, the pressure drop in the evaporator wick, artery wick, and condenser wick and the heat loss from the evaporator to the condenser through the artery wick were analyzed for the design of a 100 W AMTEC prototype. It was found that a particle diameter of 10 ${\mu}m$ is suitable for the evaporator wick to maintain a capillary pressure greater than total pressure drop in the circulation loop.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.511-516
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• 2007

This experiment was conducted to evaluate the effects of differently maturing corn hybrids on silage production and milk production per unit area in the northern part of Japan, where grain development occurs under decreasing ambient temperature. Both hybrids were harvested at the same time. The stages of maturity for the early-maturing hybrids (EH; 80 d relative maturity) and the mid-maturing hybrids (MH; 93 d relative day) were early dent and late dough stage, respectively. The plant yields for MH were higher than those for EH. The dry matter (DM) content of MH was lower than that for EH, and the effluent loss for MH silage was greater than that for EH silage. Therefore, the DM yields of prepared silage per area were similar for both treatments. Twelve multiparous mid-lactation Holstein cows ($58{\pm}13$ days in milk) were fed diets based on EH or MH silage in a crossover design with two 3-week periods. Cows were fed 3 kg of hay crop silage (DM basis) and either EH or MH silage ad libitum, and concentrates were supplied to meet NRC requirement for dairy cows. Silage DM intake for EH was found to be higher (p<0.05) than that for MH (10.0 vs. 9.1 kg/day). Milk production and milk composition for EH were similar to those for MH. Feed efficiency per total feed intake was similar in both treatments, although the feed efficiency per concentrate intake tended to be higher for the EH than that for the MH diet. These results indicate that differences in maturation in corn hybrids affect the effluent production of silage and the silage intake of dairy cows. It may be advantageous to plant early hybrid corn with a reduction in effluent production of silage as well as a reduction in purchased feed costs for dairy cows under the climatic conditions of the northern part of Japan.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.10
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• pp.2269-2274
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• 2015

In this paper, the reduced size in horizontal sizes of the narrow band pass filter (BPF) with λg/4 short stub using S shaped folded coupling structure is presented. This BPF with narrow bandwidth show flexible bandwidth with variation of the position of the stubs using the Qe(external quality factor) without changing the impedance value. On the other hand, by replacing the horizontal quarter-wavelength transmission lines with the equivalent S shaped coupled line, the BPF with reduced the size of horizontal can be realized. The proposed BPF shows the insertion loss of 1.46 dB and the return loss of 16.5 dB with bandwidth of 3.4 % at the center frequency of 5.8 GHz, respectively.

• BMB Reports
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• v.43 no.1
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• pp.57-61
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• 2010

Activator protein-1 can induce either cell survival or death, which is controlled by opposing effects of different Jun members. It is generally accepted that c-Jun is pro-apoptotic, but that JunD is anti-apoptotic in stress-exposed cells. Additionally, although there are reports suggesting that JunB plays a protective role, its role in stress-induced apoptosis remains unclear. Here, we investigated the role of JunB in $H_2O_2$-induced cell death using cells that over-expressed the protein or were transfected with si-JunB. Inhibition of JunB expression accelerated $H_2O_2$-mediated loss of mitochondrial membrane potential (MMP) and cytotoxicity. Conversely, over-expression of JunB protein led to significant inhibition of the MMP loss and cell death. The increase in JunB expression also attenuated nuclear relocation of apoptosis-inducing factor and mitochondrial Bcl-2 reduction that occurred following $H_2O_2$ exposure. These results suggest that JunB can signal survival against oxidant-mediated cell death by suppressing mitochondrial stress.

• Journal of Electrical Engineering and Technology
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• v.9 no.1
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• pp.143-153
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• 2014

This paper presents a novel application of LCC resonant converter for 60kW EV fast charger and describes development of the high efficiency 60kW EV fast charger. The proposed converter has the advantage of improving the system efficiency especially at the rated load condition because it can reduce the conduction loss by improving the resonance current shape as well as the switching loss by increasing lossless snubber capacitance. Additionally, the simple gate driver circuit suitable for proposed topology is designed. Distinctive features of the proposed converter were analyzed depending on the operation modes and detail design procedure of the 10kW EV fast charger converter module using proposed converter topology were described. The proposed converter and the gate driver were identified through PSpice simulation. The 60kW EV fast charger which generates output voltage ranges from 50V to 500V and maximum 150A of output currents using six parallel operated 10kW converter modules were designed and implemented. Using 60kW fast charger, the charging experiments for three types of high-capacity batteries were performed which have a different charging voltage and current. From the simulation and experimental results, it is verified that the proposed converter topology can be effectively used as main converter topology for EV fast charger.