• Mycobiology
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• v.28 no.4
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• pp.185-189
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• 2000

The antifungal activity of methanolic extracts of Centella asiatica and Andrographis paniculata leaves was observed against fourteen fungi, viz., Alternaria alternata, A. brassicae, A. brassicicola, A. solani, A. tenuissima, Cercospora blumae, Curvularia lunata, C. penniseti, and Drechslera monoceras, D. oryzae, D. turitica, Fusarium albizziae and F. udum. Different concentrations of the methanolic extract (1000, 2000, 3000, 5000, 7000, 10000ppm) were used. The effect of mixed leaf extract (1500 ppm of C. asiatica + 1500 ppm of A. paniculata) and its 1:2 ad 1:4 dilutions were also studied. The individual extracts of both the plants showed significant inhibitory effect on spore germination of all the fungi tested. F. udum, F. albizzae, D. oryzae, D. turtica, and D. monoceras were particularly sensitive to these extracts. In general, the extract of C. asiatica showed a higher inhibitory effect in all concentrations against all the fungi as compared to A. paniculata, except for A. brassicae A. solani, D. oryzae, D. penniseti and Curvularia sp. The inhibitory effect of extracts increased when they were used in combination with or without dilutions against A. brassicicola, A. solani A. brassicae, A. alternata, A. tenussima, C. blumae, C. lunata, C. penniseti and Curvularia species. Higher efficacy of active ingredient of these extracts under field condition is envisaged against plant pathogens.

• Journal of Life Science
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• v.31 no.4
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• pp.389-399
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• 2021

This study determined the effects of Centella asiatica leaf on H₂O₂ induced cell cycle arrest, mitochondrial activity, and proinflammatory cytokine production in human dermal fibroblast (HDF) cells. We used an 80% methanol extract of C. asiatica, its ethyl acetate fraction, and asiaticoside, the major constituent of C. asiatica. The C. asiatica extract, its ethyl acetate fraction, and asiaticoside attenuated G1 cell cycle-arrest and the apoptotic effect caused by H₂O₂-induced oxidative stress. The cells treated with C. asiatica extract, its ethyl acetate fraction, and asiaticoside secreted lower levels of TNF-α and IL-6. The antioxidant effect of asiaticoside was higher than that of C. asiatica extract and its ethyl acetate fraction. Treatment with C. asiatica extract, its ethyl acetate fraction, and asiaticoside also increased the mitochondrial membrane potential and restored normal mitochondrial morphology. Following H₂O₂ stress induction, cells treated with C. asiatica extract, its ethyl acetate fraction, and asiaticoside showed increased mitochondrial oxygen consumption rates and decreases in the TLR4-MyD88-TRAF6-p65 pathway activity. These findings suggest that C. asiatica extract, its ethyl acetate fraction, and asiaticoside have antioxidant and anti-inflammatory effects, as well as the ability to control the mitochondrial activities of HDF cells.

• Preventive Nutrition and Food Science
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• v.21 no.3
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• pp.197-201
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• 2016

Helicobacter pylori infection is associated with an increased risk of developing upper gastrointestinal tract diseases. However, treatment failure is a major cause of concern mainly due to possible recurrence of infection, the side effects, and resistance to antibiotics. The aim of this study was to investigate the activities of Centella asiatica leaf extract (CAE) against H. pylori both in vitro and in vivo. The minimum inhibitory concentrations (MICs) against 55 clinically isolated strains of H. pylori were tested using an agar dilution method. The MICs of CAE ranged from 0.125 mg/mL to 8 mg/mL, effectiveness in inhibiting H. pylori growth was 2 mg/mL. The anti-H. pylori effects of CAE in vivo were also examined in H. pylori-infected C57BL/6 mice. CAE was orally administrated once daily for 3 weeks at doses of 50 mg/kg and 250 mg/kg. CAE at the 50 mg/kg dose significantly reduced H. pylori colonization in mice gastric mucosa. Our study provides novel insights into the therapeutic effects of CAE against H. pylori infection, and it suggests that CAE may be useful as an alternative therapy.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.351-358
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• 2021

The study was investigated to compare growth characteristics, the antioxidant activity, and the triterpenoid content of two Centella asiatica cultivars ('Giant Tiger Care' and 'Good Tiger Care'). At 41 days after transplanting, lengths of leaf and petiole were significantly longer in 'Good Tiger Care' than in 'Giant Tiger Care'. However, the growth characteristics (leaf area, petiole thickness, petiole length, and weight) were greater in 'Giant Tiger Care' than in 'Good Tiger Care' at 104 days after transplanting (harvest time). Antioxidant activity and total phenol content in four extracts (WE, water extract; HWE, hot water extract; 50E, 50% EtOH extract; 70E, 70% EtOH extract) of the two cultivars were high in 70E of 'Giant Tiger Care'. As a result of the triterpenoids analysis, the major triterpenoids of the two cultivars were identified as madecassoside and asiaticoside. The total triterpenoid content was high in 50E and 70E of 'Giant Tiger Care' and 'Good Tiger Care', respectively but the total triterpenoid content was highest in Good Tiger Care'. However, at the 104 days after planting, the yield of 'Giant Tiger Care' was three times higher than that of 'Good Tiger Care'. In addition, the total triterpenoid content that can be produced in the same cultivation area (3.3m²) was 2.459mg in 50E of 'Giant Tiger Care', which was ~2.2times higher than that of 70E of 'Good Tiger Care' (1.103 mg). Thus, it is considered economical to cultivate 'Giant Tiger Care' which has the highest antioxidant activity and high total triterpenoid production per cultivation area.