• Molecules and Cells
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• v.25 no.4
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• pp.487-493
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• 2008

HoxB4 has been shown to enhance hematopoietic engraftment by hematopoietic stem cells (HSC) from differentiating mouse embryonic stem cell (mESC) cultures. Here we examined the effect of ectopic expression of HoxB4 in differentiated human embryonic stem cells (hESCs). Stable HoxB4-expressing hESCs were established by lentiviral transduction, and the forced expression of HoxB4 did not affect stem cell features. HoxB4-expressing hESC-derived CD34+ cells generated higher numbers of erythroid and blast-like colonies than controls. The number of CD34+ cells increased but CD45+ and KDR+ cell numbers were not significantly affected. When the hESC derived CD34+ cells were transplanted into $NOD/SCID{\beta}2m-/-$ mice, the ectopic expression of HoxB4 did not alter their repopulating capacity. Our findings show that overexpression of HoxB4 in differentiating hESCs increases hematopoietic colony formation and hematopoietic cell formation in vitro, but does not affect in vivo repopulation in adult mice hosts.

• Molecules and Cells
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• v.37 no.9
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• pp.685-690
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• 2014

Osteoclasts are large polykaryons that have the unique capacity to degrade bone and are generated by the differentiation of myeloid lineage progenitors. To identify the genes involved in osteoclast development, we performed microarray analysis, and we found that carboxypeptidase E (CPE), a prohormone processing enzyme, was highly upregulated in osteoclasts compared with their precursors, bone marrow-derived macrophages (BMMs). Here, we demonstrate a novel role for CPE in receptor activator of NF-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. The overexpression of CPE in BMMs increases the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinuclear osteoclasts and the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1), which are key regulators in osteoclastogenesis. Furthermore, employing CPE knockout mice, we show that CPE deficiency attenuates osteoclast formation. Together, our data suggest that CPE might be an important modulator of RANKL-induced osteoclast differentiation.

• Korean Journal of Environmental Biology
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• v.20 no.1
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• pp.35-39
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• 2002

The significance of cell ploidy and repair ability for the radioprotective efficiency of cysteamine was studied in DNA repair - proficient and repair - deficient yeast cells irradiated $^{60}C0\;\gamma-rays.$ Results have been obtained for the cell survival of two groups of yeasts-diplont and haplont cells, both in haploid and diploid states. For diploid Saccharomyces cerevisiae yeast cells, the correlation between the radio-protective action of cysteamine and the cell repair capacity was demonstrated. Such a correlation was not clearly expressed for haploid yeast cells. In addition, evidence was obtained indicating that the degree of the radioprotective action was independent of the number of chromosome sets in haplont yeast Pichia guilliermondii cells and in some radiosensitive mutants defective in the diploid-specific recovery. It is concluded on this basis that the radioprotective action may involve the cellular recovery process, which may be mediated by a recombination-like mechanism, for which the diploid state is required. The results obtained clearly show that the radioprotective effect was dependent on DNA repair status and indicate that the mechanism of the radioprotective action may be realized on the level of primary radiation damage production as well as on the level of postradiation recovery from potentially lethal radiation damage.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.8C
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• pp.771-778
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• 2003

A mobile station in a cell receives interference signals from neighboring base stations. Due to the interference signals, the forward link capacity of the cellular system is reduced. Pilot signal received from a neighboring base station is one of the interference signals. Since the pilot signal is a known signal for all the mobile stations, the pilot interference signal can be cancelled by interference cancellation techniques. In this paper, we propose a demodulator with interference canceller to cancel pilot interference signals received from neighboring base stations. The simulation results show that Eb/No value to obtain BER = 10-2 is lowered by 3㏈ in the propose demodulator.

• Journal of Communications and Networks
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• v.16 no.2
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• pp.121-129
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• 2014

The trend of an increasing demand for a high-quality user experience, coupled with a shortage of radio resources, has necessitated more advanced wireless techniques to cooperatively achieve the required quality-of-experience enhancement. In this study, we investigate the critical problem of rate splitting in heterogeneous cellular networks, where concurrent transmission, for instance, the coordinated multipoint transmission and reception of LTE-A systems, shows promise for improvement of network-wide capacity and the user experience. Unlike most current studies, which only deal with spectral efficiency enhancement, we implement an optimal rate splitting strategy to improve both spectral efficiency and energy efficiency by exploring and exploiting cooperation diversity. First, we introduce the motivation for our proposed algorithm, and then employ the typical cooperative bargaining game to formulate the problem. Next, we derive the best response function by analyzing the dual problem of the defined primal problem. The existence and uniqueness of the proposed cooperative bargaining equilibrium are proved, and more importantly, a distributed algorithm is designed to approach the optimal unique solution under mild conditions. Finally, numerical results show a performance improvement for our proposed distributed cooperative rate splitting algorithm.

• Korean Journal of Microbiology
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• v.27 no.1
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• pp.10-15
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• 1989

The plasmid pKOneo, containing SV40 transcriptional promoter, has been used in the mouse tumorigenicity assay for oncogene studies. This assay employs a cotransfection of NIG3T3 fibroblast cells with the desired DNA and the plasmid pKOneo. This oncogene assay, however, has been speculated due to the SV40 transcriptional promoter in the plasmid pKOneo. This research was designed to investigate if the plasmid pKOneo alone is capable of transforming NiH3T3 fibroblast cells. The NIH3T3 subclones were established after the NIH3T3 cells were transfected with the plasmid pKOneo alone. The estabilished NIH3T3 subclones, containing the exogeneous plasmid pKOneo in their chromosomes, were examined for their expression of transformation-associated parameters. The results indicate that this plasmid pKOneo alone has positive effects on transformation of NIH3T3 cells after integration into cellular chromosomes.

• Proceedings of the IEEK Conference
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• summer
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• pp.204-208
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• 2004

Embedded business will be expanded market more and more since customers seek more wearable and ubiquitous systems. Cellular telephones, PDAs, notebooks and portable multimedia devices could bring higher microprocessor revenues and more rewarding improvements in performance and functions. Increasing battery capacity is still creeping along the roadmap. Until a small practical fuel cell becomes available, microprocessor developers must come up with power-reduction methods. According to MPR 2003, the instruction and data caches of ARM920T processor consume $44\%$ of total processor power. The rest of it is split into the power consumptions of the integer core, memory management units, bus interface unit and other essential CPU circuitry. And the relationships among CPU, peripherals and caches may change in the future. The processor working on higher operating frequency will exact larger cache RAM and consume more energy. In this paper, we propose advanced low power trace cache which caches traces of the dynamic instruction stream, and reduces cache access times. And we evaluate the performance of the trace cache and estimate the power of the trace cache, which is compared with conventional cache.

• Preventive Nutrition and Food Science
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• v.20 no.3
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• pp.176-182
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• 2015

Abalone protein was hydrolyzed by enzymatic hydrolysis and the optimal enzyme/substrate (E/S) ratios were determined. Abalone protein hydrolysates (APH) produced by Protamex at E/S ratio of 1:100 showed angiotensin I converting enzyme inhibitory activity with $IC_{50}$ of 0.46 mg/mL, and APH obtained by Flavourzyme at E/S ratio of 1:100 possessed the oxygen radical absorbance capacity value of $457.6{\mu}M$ trolox equivalent/mg sample. Flavourzyme abalone protein hydrolysates (FAPH) also exhibited $H_2O_2$ scavenging activity with $IC_{50}$ of 0.48 mg/mL and $Fe^{2+}$+ chelating activity with $IC_{50}$ of 2.26 mg/mL as well as high reducing power. FAPH significantly (P<0.05) protected $H_2O_2$-induced hepatic cell damage in cultured hepatocytes, and the cell viability was restored to 90.27% in the presence of FAPH. FAPH exhibited 46.20% intracellular ROS scavenging activity and 57.89% lipid peroxidation inhibition activity in cultured hepatocytes. Overall, APH may be useful as an ingredient for functional foods.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.5
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• pp.971-980
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• 1994

The need increased capacity in the cellular system has resulted in the adoption of digital technology with CDMA as the channel access method. It has been recognized that the distinction of the base station is important for its performance in CDMA, since the same spreading sequences are used by the all base stations. Time offset of the pseudo-random noise binary code are used to distinguish signals received at a mobile station from different base station. But the start of the zero offset PN sequence is chosen arbitrary without the background of the systematic and mathematical elaboration. This paper proposes a mothed that define the start of the zero offset PN sequence mathematically. This paper also discusses a method that can easily calculate the time offset of the received spreading sequence with respect to the zero offset PN sequence.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.6C
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• pp.551-559
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• 2005

Estimation of unknown signal parameters with sensor array measurements has been investigated quite extensively. Also, there has been in recent years an explosive increase in the number of mobile users in wireless cellular systems, thus contributing to growing levels of multi-user interference. To overcome this problem, application of adaptive antenna array techniques to further increase the channel capacity has been discussed. In this paper, a new model of locally scattered signals in the vicinity of mobiles is proposed by defining the mean steering vector and is manipulated mathematically for several distributions. Under this model an estimation method of the angle of arrival(AOA) is investigated based on a weighted subspace fitting(WSF) technique. Statistical analysis and simulations are also considered.