• Restorative Dentistry and Endodontics
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• 제45권4호
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• pp.52.1-52.11
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• 2020

Objectives: Yttria-stabilized tetragonal phase zirconia has been used as a dental restorative material for over a decade. While it is still the strongest and toughest ceramic, its translucency remains as a significant drawback. To overcome this, stabilizing the translucency zirconia to a significant cubic crystalline phase by increasing the yttria content to more than 8 mol% (8YTZP). However, the biocompatibility of a high amount of yttria is still an important topic that needs to be investigated. Materials and Methods: Commercially available 8YTZP plates were used. To enhance cell adhesion, proliferation, and differentiation, the surface of the 8YTZP is sequentially polished with a SiC-coated abrasive paper and surface coating with type I collagen. Fibroblast-like cells L929 used for cell adherence and cell proliferation analysis, and mouse bone marrow-derived mesenchymal stem cells (BMSC) used for cell differentiation analysis. Results: The results revealed that all samples, regardless of the surface treatment, are hydrophilic and showed a strong affinity for water. Even the cell culture results indicate that simple surface polishing and coating can affect cellular behavior by enhancing cell adhesion and proliferation. Both L929 cells and BMSC were nicely adhered to and proliferated in all conditions. Conclusions: The results demonstrate the biocompatibility of the cubic phase zirconia with 8 mol% yttria and suggest that yttria with a higher zirconia content are not toxic to the cells, support a strong adhesion of cells on their surfaces, and promote cell proliferation and differentiation. All these confirm its potential use in tissue engineering.

• Steel and Composite Structures
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• 제49권4호
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• pp.393-405
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• 2023

In this paper, the energy absorption capability of a novel cruciform composite lattice structure was evaluated through the simulation of compression tests. For this purpose, several test samples of Polylactic acid cellular reinforced with continuous glass fibers were prepared for compression testing using the additive manufacturing method of material extrusion. Using a conventional path design for material extrusion, multiple debonding is probable to be occurred at the joint regions of adjacent cells. Therefore, an innovative printing path design was proposed for the cruciform lattice structure. Afterwards, quasistatic compression tests were performed to evaluate the energy absorption behaviour of this structure. A finite element model based on local material property degradation was then developed to verify the experimental test and extend the virtual test method. Accordingly, different combinations of unit cells' dimensions using the design of the experiment were numerically proposed to obtain the optimal configuration in terms of the total absorbed energy. Having brilliant energy absorption properties, the studied cruciform lattice with its optimized unit cell dimensions can be used as an energy absorber in crashworthiness applications. Finally, a cellular structure will be suitable with optimal behavior in crush load efficiency and high energy absorption.

• Child Health Nursing Research
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• 제19권4호
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• pp.270-281
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• 2013

목적 본 연구의 목적은 초등학생을 위한 매체 중독 예방 프로그램을 개발하고, 그 효과를 검증하는 것이다. 방법 선행 연구와 관련 문헌 및 전문가의 자문을 토대로 초등학생을 위한 매체 중독 예방 프로그램을 개발하였다. 초등학교 4-6학년생 58명(실험군 29명, 대조군 29명)을 대상으로 텔레비전 중독, 인터넷 중독, 휴대전화 중독 및 충동성에 대한 사전조사를 실시하였고, 실험군에게는 주 2-3회, 4주간 10회기의 매체 중독 예방 프로그램을 실시한 후 사후 조사와 프로그램 종료 1개월 후 추후조사를 실시하였으며, 대조군에게는 동일한 시기에 설문조사를 실시하였다. 결과 매체 중독 예방 프로그램에 참여한 실험군이 대조군보다 텔레비전 중독 점수가 유의하게 감소하였다. 그러나 인터넷 중독, 휴대전화 중독, 충동성은 실험군과 대조군의 프로그램 실시 전후에 따른 변화 양상이 다르지 않았다. 결론 초등학생을 위한 매체 중독 예방 프로그램은 텔레비전 중독 예방에 효과적이었다. 매체 중독 예방 프로그램은 초등학생의 올바른 텔레비전 사용 습관을 기르도록 도와서, 이후 심각한 문제 행동을 방지할 수 있을 것이다.

• Spatial Information Research
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• 제18권5호
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• pp.133-142
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• 2010

실내 화재와 같은 재난, 재해시의 보행자의 행태를 모델링하거나 건축물의 구조를 분석하기 위해 지난 수십 년간 다양한 보행모델, 또는 화재대피모델들이 연구되어 왔다. 그러나 최근까지 개발된 모델은 대피 시 구조물들에 의해 보행자의 시야가 제한되는 것을 고려하고 있지 않다. 보행자의 시야는 대피에 영향을 미치는 중요한 요인 중 하나이므로, 이를 고려해야 현실적인 시뮬레이션 결과를 도출할 수 있다. 대피시뮬레이션에서 보행자의 시야에 대한 영향을 고려하는 방법은 시야의 제한 정도에 따라서 보행자의 대피 속도를 다르게 하는 것이다. 본 연구에서는 보행자의 시야에 따라 서로 다른 대피 속도를 갖게 하기 위해서 cellular automata를 이용한 floor field 모델을 기반으로 개선된 알고리즘을 제시하였다. 공간구문론(space syntax)을 활용하여 시야에 따라 공간을 분할하고, 동시다발적인 움직임 대신 분할된 공간별로 다른 이동속도를 갖게 하는 개선된 알고리즘을 구현하여 대피의 행태를 적절하게 모델링할 수 있게 하였다. 또한, 본 연구에서는 추후 실내 센서와의 연동을 통한 실시간 시뮬레이션 시스템으로의 개발을 위하여 공간DBMS를 이용한 3차원 보행자 시뮬레이터의 구현과정을 예시하였다. 캠퍼스 건물을 대상으로 개선된 알고리즘의 시뮬레이션 테스트를 수행하였다.

• The Journal of Advanced Prosthodontics
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• 제6권4호
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• pp.285-294
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• 2014

PURPOSE. The purpose of this study was to evaluate the properties of a porous zirconia scaffold coated with bioactive materials and compare the in vitro cellular behavior of MC3T3-E1 preosteoblastic cells to titanium and zirconia disks and porous zirconia scaffolds. MATERIALS AND METHODS. Titanium and zirconia disks were prepared. A porous zirconia scaffold was fabricated with an open cell polyurethane disk foam template. The porous zirconia scaffolds were coated with ${\beta}$-TCP, HA and a compound of ${\beta}$-TCP and HA (BCP). The characteristics of the specimens were evaluated using scanning electron microscopy (SEM), energy dispersive x-ray spectrometer (EDX), and x-ray diffractometry (XRD). The dissolution tests were analyzed by an inductively coupled plasma spectrometer (ICP). The osteogenic effect of MC3T3-E1 cells was assessed via cell counting and reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS. The EDX profiles showed the substrate of zirconia, which was surrounded by the Ca-P layer. In the dissolution test, dissolved $Ca^{2+}$ ions were observed in the following decreasing order; ${\beta}$-TCP > BCP > HA (P<.05). In the cellular experiments, the cell proliferation on titanium disks appeared significantly lower in comparison to the other groups after 5 days (P<.05). The zirconia scaffolds had greater values than the zirconia disks (P<.05). The mRNA level of osteocalcin was highest on the non-coated zirconia scaffolds after 7 days. CONCLUSION. Zirconia had greater osteoblast cell activity than titanium. The interconnecting pores of the zirconia scaffolds showed enhanced proliferation and cell differentiation. The activity of osteoblast was more affected by microstructure than by coating materials.

• BMB Reports
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• 제44권12호
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• pp.793-798
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• 2011

Recently, pluripotency induction or cellular reprogramming by introducing critical transcription factors has been extensively studied, but has been demonstrated only in vitro. Based on reports that Oct4 is critically involved in transforming neural stem cells into pluripotent cells, we used the lentiviral vector to introduce the Oct4 gene into the hippocampal dentate gyrus (DG) of adult mice. We examined whether this manipulation led to cellular or behavioral changes, possibly through processes involving the transformation of NS cells into pluripotent cells. The Oct4 lentivirus-infused group and the green fluorescent protein lentivirus-infused group showed a similar thickness of the DG and a comparable level of synaptophysin expression in the DG. Furthermore, our behavioral analyses did not show any differences between the groups concerning exploratory activity, anxiety, or memory abilities. This first trial for pluripotency induction in vivo, despite negative results, provides implications and information for future studies on in vivo cellular reprogramming.

• Molecules and Cells
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• 제28권1호
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• pp.37-42
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• 2009

Iron binding lactoferrin (Lf) is involved in the control of cell cycle progression. However, the molecular basis underlying the effects of Lf on cell cycle control, as well as its target genes, remains incompletely understood. In this study, we have demonstrated that a relatively low level of ironsaturated Lf, Lf($Fe^{3+}$), can stimulate S phase cell cycle entry, and requires Akt activation in MCF-7 cells. Lf($Fe^{3+}$) immediately induced Akt phosphorylation at Ser473, which subsequently induced the phosphorylation of two G1-checkpoint Cdk inhibitors, $p21^{Cip/WAF1}$ and $p27^{kip1}$. The Lf($Fe^{3+}$)-induced phosphorylation of Cdk inhibitors impaired their nuclear import behavior, thereby inducing cell cycle progression. However, the treatment of cells with a PI3K inhibitor, LY294002, almost completely blocked Lf($Fe^{3+}$)-stimulated cell cycle progression. LY294002 treatment abrogated Lf($Fe^{3+}$)-induced Akt activation, and prevented the cytoplasmic localization of $p27^{kip1}$. Higher levels of $p21^{Cip/WAF1}$ were also detected in the cytoplasmic sub-cellular compartment as a measure of cellular response to Lf($Fe^{3+}$). Consequently, the degree of phosphorylation of retinoblastoma protein was enhanced in response to Lf($Fe^{3+}$). Therefore, we conclude that Lf($Fe^{3+}$), as a potential antagonist of Cdk inhibitors, can facilitate the functions of E2F during progression to S phase via the Akt signaling pathway.

• 대한기계학회논문집A
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• 제34권11호
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• pp.1659-1666
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• 2010

WBD(wire-woven bulk diamond)라고 명명한 와이어로 직조된 다공질 금속의 새로운 형태를 소개한다. WBD 는 기계적 열 특성과 공학적 응용면에서 몇 년 전에 소개되어진 WBK(wire-woven bulk Kaogme)와 같이 나선형 와이어로 구성된다. WBK 에서는 각 교차점에서 3 개의 와이어가 접하나 WBD 에서는 4 개의 와이어가 서로 접하여 배열된다. 압축하중 하에서 WBD 의 기계적 거동을 조사하고 그 결과를 WBK 와 비교하였다. 같은 세장비(d/c)에서 WBD 의 밀도 및 강도는 WBK 의 약 2 배이나, WBD 의 강성도는 WBK 보다 그만큼 높지 않다.

• 한국재료학회지
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• 제10권7호
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• pp.489-492
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• 2000

0.5%C-25.0%Cr-1.0%Si(합금1), 0.5%C-5.0%Cr-1.0%Si(합금2) 및 2.0%C-5.0%Cr-1.0%Si(합금3)의 3종류 크롬백주철에 있어서 기지조직 및 탄화물에 분푀도는 Cr 및 Si의 거동을 연구하였다. 15kg 용량의 고주파 유도용해로에 선철, 고철, Fe-Cr, Fe-Si 등을 장입시켜 용해시킨후 슬래그를 제거시키고 $1550^{\circ}C$에서 펩 주형에 주입시킨후 실온까지 냉각시켜 SEM으로 응고조직을 관찰하였으며 EPMA분석을 통하여 Cr 및 Si 의 분포거동을 관찰하였다. 합금1의 경우 초정으로 $\delta$페라이트가 정출후 $\delta$페라이트와 용액의 입계에서 $\delta$페라이트와 $M_7C_3$탄화물이 공정으로 정출하였으며 합금2의 경우 용액에서 초정으로 거의 $\delta$페라이트가 정출된 수 극히 일부분만이 $\delta$페라이트와 $M_7C_3$탄화물의 공정으로 정출하였다. 반면 합금 3의 경우 오스테나이트가 초정으로 정출된 후 오스테나이트와 $M_3C$탄화물이 공정으로 정출하였다. Cr은 주로 $M_7C_3$ 및 $M_3C$탄화물에 , 그리고 Si는 기지조직에 선택적으로 분배되었으며 Cr의 기지조직에 대한 분배계수는 0.56-0.68, 그리고 Si는 1.12-1.28의 범위에 걸쳐있었다. 또한 Cr의 기지조직에 대한 분배계수는 C 함량이 2.0%일때가 0.5%의 경우보다 낮았으며 $M_7C_3$탄화물내의 Cr 함량은 Cr함량이 25.0% 일때가 5.0%의 경우보다 높은값을 나타내었다. 나타내었다.

• 대한세포병리학회지
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• 제8권1호
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• pp.62-68
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• 1997

Acinic cell carcinoma is a slow-growing solid neoplasm of salivary gland. Although their cytological and histological finding is bland-looking, their biological behavior is unpredictable. We experienced two cases of acinic cell carcinoma of the salivary gland diagnosed by fine needle aspiration biopsy and confirmed by tissue examination. They showed different clinical courses. We compared their cytologic and histologic findings. The first case was a right preauricular mass in a 58 year-old female of 3 years duration. The cytologic smear revealed sheets or small clusters of monotonous cells mimicking normal serous acinar cells with little cellular pleomorphism. She underwent superficial parotid lobectomy. The tumor was a well demarcated 1.5cm sized nodular mass without infiltration into surrounding parenchyme. The second case was a left submandibular mass in a 23 year-old male of 4 years duration. The smear showed more severe pleomorphism of the tumor cells than those of previous case. Excisional biopsy was done. The excised tumor was $5.5{\times}3.5{\times}3cm$ sized multilobulated solid mass with invasion into surrounding parenchyme. The tumor recurred after 20months, thus total excision of the mass and modified radical neck dissection was carried out. From the above findings, cytologic atypism, infiltrative growth pattern and type of initial therapy may be correlated with biologic behavior.