• KSBB Journal
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• v.24 no.2
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• pp.182-188
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• 2009

Lactobacillus spp., primary members of probiotics, have significant benefits for health and well-being of human. In this study Lactobacillus strains representing six species (L. paracasei KLB58, L. fermentum MS79 and KLB282, L. plantarum KLB213, L. gasseri KLB238, and L. reuteri KLB270) isolated from Korean adults were electrotransformed with plasmid pNCKH104. To determine optimal electrotransformation conditions, various conditions including cell wall weakening agent, electroporation buffer, electric field strength and time constant were tested for each strain. Overall, high transformation efficiency of approximately 2.5 ${\times}$ $10^3$ ${\sim}$ 5.5 ${\times}$ $10^4$ CFU/${\mu}g$ DNA was obtained where conditions of 0.5 M sucrose electroporation buffer, 1.8 kV pulse voltage and 5 ms time constant were applied. The common conditions developed in this study will make transformation of various Lactobacillus spp. easier than previous procedures.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.405-431
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• 2008

Cuticle micromorphology of 22 species of Quercus and outgroup were examined by the SEM. Twenty-two species selected each two or three species in all section of the genus Quercus. The genus Trigonobalanus and Alnus are selected as outgroups. Ten characters of the inner surface and eight characters of the outer surface of the cuticle have been described. Some characters, such as the present of papillae, arrangement of subsidiary cell, shape of anticlinal cell wall are considered important character for infrageneric classification. A parsimony analysis of 18 characters resulted in 72 most parsimonious trees with its lengths of 66 steps. The topology obtained from the analysis showed two major lineages. Subgenus Cyclobalanopsis formed one clade by 75% and subgenus Quercus formed another clade by 57% bootstrap value. Based on the cuticle morphology, the two subgenus delimitation of Camus was supported. However, sect. Erythrobalanus and sect. Cerris formed one group, and sect. Lepidobalanus formed polytomy.

• Korean Journal of Microbiology
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• v.27 no.4
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• pp.357-365
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• 1989

Induction conditions for autolysis and autoplast formation of thermophilic Clostridium thermohydrosulfuricum were studied. The cells in the initial exponential growth phase were well autolysed in Tris-HCl buffer or inorganic buffers containing univalents, such as $K^{+}$ and $Na^{+}$ , and chemicals such as cysteine-HCl, sorbitol and glycerol. Meanwhile, autolysis induction was slightly inhibited by divalents, such as $Mg^{2+}, Mn^{2+}, Ca^{2+}, Ni^{2+}$, and strongly by divalents, such as $Fe^{2+}, Cu^{2+}$ and citric acid. The autolysis was stimulated when the cells were grown in the medium containing ampicillin that inhibites cell wall synthesis, meanwhile, it was slightly inhibited by nucleic acids and protein synthesis inhibitors. The optimal pH and temperature for the induction of autolysis were 7.5 and $60^{\circ}C$, respectively. On the other hand, the cells were autoplasted without lysozyme treatment during autolysis due to the stabilization of protoplasmic membrane in the presence of divalents such as $Mg^{2+}, Mn^{2+}, Ca^{2+}, Ni^{2+}$. Autoplast formation was mostly induced at $37^{\circ}C$ in 50mM Tris-HCl buffer (pH 7.5) containing 20 mM $MgCl^{2}$ and 0.3 M glycerol, and in the late exponential growth phase growing cell.

• Journal of the Korea Institute of Building Construction
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• v.18 no.1
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• pp.17-24
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• 2018

The pullout test, a nondestructive testing(NDT), for pre-installed inserts is perhaps the most widely used technique to estimate the in-situ compressive strength of concrete. It measures the force needed to pullout a standardized metal insert embedded into concrete members. The pullout test was certified by the American Society for Testing and Materials(ASTM) and Canadian Standards Association(CSA) as a reliable method for determining the strength of concrete in concrete structures under construction. To easily estimate the strength of ultra-high-strength concrete, a simplified pullout tester, primarily composed of a standard 12mm bolt with a groove on the shaft as a break-off bolt, an insert nut, and a hydraulic oil pump without a load cell, was proposed. Four wall and two slab specimens were tested for two levels of concrete strength, 80MPa and 100MPa, using a simplified pullout tester with a load cell to verify the advantages of the pullout test and simplified pullout test. The compressive strength of concrete, pullout load, and the rupture of the break-off bolt were measured 11 times, day 1 to 7, 14, 21, 28, and 90. The correlation of the pullout load and the compressive strength of each specimen show a higher degree of reliability. Therefore, a simplified pullout test can be used to evaluate the in-place strength of ultra-high-strength concrete in structures. The prediction equation for the groove diameter of the break-off bolt(y) with the concrete strength(x) was proposed as y=0.0184x+5.4. The results described in this research confirm the simplified pullout's utility and potential for low cost, simplicity, and convenience.

• Applied Biological Chemistry
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• v.28 no.3
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• pp.174-181
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• 1985

Ultrastructural changes in the chloroplast of apple fruits was examined with Malus Pumila Mill var. Fugi harvested during preclimacteric stage the subatmospheric pressure storage condition at 380mmHg, $20^{\circ}C$. Electron micrographs obtained from the fruits stored at 760mmHg, $20^{\circ}C$ indicated that the initiation of ultrastructural changes is in parallel with the onset of respiratory climacteric. Chloroplast of the apple fruits stored for 25 days is vacuolated and the lamellar system are a little disorganized. Chloroplast of the apple fruits stored for 50 days is showing extensive vacuolation, the lamellar system are completely disorganized and the plasma membrane has pulled away from the cell wall. In the case of subatmospheric presssure storage caused to delay the onset of ultrastructural changes in chloroplast such as vacuolation, disorganization of lamellar system and dispersal of stroma lamella about 2 months. These results suggested that ripening of apple fruits is correlated not only to the activity of respiration but to the involvement of ultrastructural changes in fruit cells. The evidences obtained here led to the conclusion that subatmospheric pressure treatment extends storage life of apple fruits by inhibiting ultrastructural changes of cell organelles in concomitance with respiratory activity.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.590-597
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• 2013

Principle component and emission localization of volatile compounds were investigated according to scent intensity of rose flower. Scent intensity in cultivars and bred-line of Rosa hybrida was divided into three levels; light ('Feel Lip', 'Venus Berry'), medium ('GR07-135'), strong ('Honey Blue'). The major volatile compounds were different depending on the cultivars and selected line; 3,5-dimethoxytoluene (DMT), benzene, 1,3,5-trimethoxy ('Feel Lip'), megastigma-4,6(Z),8(E)-triene ('Venus Berry'), DMT, benzene,1-ethenyl-4-methoxyand, phenylethylalcohol ('GR07-135') and germacrene-D, DMT ('Honey Blue'). The adaxial epidermal cells were conical papillate shape, whereas the abaxial epidermal cells were flat shape. The adaxial epidermal cells of 3 cultivars and 1 selected line were surrounded by thick cell wall and covered by waxy cuticle of 2 cultivars and 1 selected line (except 'Honey Blue'). The adaxial epidermal cells contained starches in 'Feel Lip', osmiophlic droplets in 'Venus Berry', starchs, plastids, vacuoles in 'GR07-135' and plastoglobules, plastids, vacuoles in 'Honey Blue'. Based on these results, it appears that plastids and vacuoles in adaxial epidermal cells with conical papillate shape are associated production and emission of volatile compounds in scent R. hybrida.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.611-618
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• 2001

The ethanol extract of Dryopteris crassirhizoma Nakai showed strong growth inhibition against 5 strains of Listeria monocytogenes at the concentrations of $100{\sim}500$ ppm and the minimum inhibitory concentration of n-hexane fraction was under 50 ppm. The D8-2-5 fraction isolated from n-hexane fraction of Dryopteris crassirhizoma Nakai showed a strong bactericidal activity on 5 strains of L. monocytogenes at 20 ppm level in tryptic soy broth medium. At the level, the viable count was reduced $4{\sim}6$ log cycle compared to initial cell number. Observation by the measurement of adenosine triphosphate (ATP) contents and transmission electron microscope showed that disruptions of the cell wall and elution of intracellular ATP are assumed to be due to the bactericidal activity. In addition, the n-hexane fraction of Dryopteris crassirhizoma Nakai showed the strong growth inhibitions at 50 ppm on Vibrio parahaemolyticus and Bacillus cereus, and at 25 ppm on Staphylococcus aureus.

• Journal of Ginseng Research
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• v.47 no.1
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• pp.81-88
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• 2023

Background: Air pollution has led to an increased exposure of all living organisms to fine dust. Therefore, research efforts are being made to devise preventive and therapeutic remedies against fine dust-induced chronic diseases. Methods: Research of the respiratory protective effects of KRG extract in a particulate matter (PM; aerodynamic diameter of <4 ㎛) plus diesel exhaust particle (DEP) (PM4+D)-induced airway inflammation model. Nitric oxide production, expression of pro-inflammatory mediators and cytokines, and IRAK-1, TAK-1, and MAPK pathways were examined in PM4-stimulated MH-S cells. BALB/c mice exposed to PM4+D mixture by intranasal tracheal injection three times a day for 12 days at 3 day intervals and KRGE were administered orally for 12 days. Histological of lung and trachea, and immune cell subtype analyses were performed. Expression of pro-inflammatory mediators and cytokines in bronchoalveolar lavage fluid (BALF) and lung were measured. Immunohistofluorescence staining for IRAK-1 localization in lung were also evaluated. Results: KRGE inhibited the production of nitric oxide, the expression of pro-inflammatory mediators and cytokines, and expression and phosphorylation of all downstream factors of NF-κB, including IRAK-1 and MAPK/AP1 pathway in PM4-stimulated MH-S cells. KRGE suppressed inflammatory cell infiltration and number of immune cells, histopathologic damage, and inflammatory symptoms in the BALF and lungs induced by PM4+D; these included increased alveolar wall thickness, accumulation of collagen fibers, and TNF-α, MIP2, CXCL-1, IL-1α, and IL-17 cytokine release. Moreover, PM4 participates induce alveolar macrophage death and interleukin-1α release by associating with IRAK-1 localization was also potently inhibited by KRGE in the lungs of PM4+D-induced airway inflammation model. KRGE suppresses airway inflammatory responses, including granulocyte infiltration into the airway, by regulating the expression of chemokines and inflammatory cytokines via inhibition of IRAK-1 and MAPK pathway. Conclusion: Our results indicate the potential of KRGE to serve as an effective therapeutic agent against airway inflammation and respiratory diseases.

• Nuclear Engineering and Technology
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• v.55 no.11
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• pp.4274-4281
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• 2023

Blanket is of vital importance for engineering application of the fusion reactor. Nuclear heat deposition in materials is the main heat source in blanket structure. In this paper, the three-dimensional method for thermal-hydraulics/neutronics coupling analysis is developed and applied for the full-scale module of the helium-cooled ceramic breeder tritium breeding blanket (HCCB TBB) designed for China Fusion Engineering Test Reactor (CFETR). The explicit coupling scheme is used to support data transfer for coupling analysis based on cell-to-cell mapping method. The coupling algorithm is realized by the user-defined function compiled in Fluent. The three-dimensional model is established, and then the coupling analysis is performed using the paralleled Coupling Analysis of Thermal-hydraulics and Neutronics Interface Code (CATNIC). The results reveal the relatively small influence of the coupling analysis compared to the traditional method using the radial fitting function of internal heat source. However, the coupling analysis method is quite important considering the nonuniform distribution of the neutron wall loading (NWL) along the poloidal direction. Finally, the structure optimization of the blanket is carried out using the coupling method to satisfy the thermal requirement of all materials. The nonlinear effect between thermal-hydraulics and neutronics is found during the blanket structure optimization, and the tritium production performance is slightly reduced after optimization. Such an adverse effect should be thoroughly evaluated in the future work.

• Tuberculosis and Respiratory Diseases
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• v.47 no.1
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• pp.66-76
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• 1999

Background : To assess the utility of HRCT in the evaluation of visceral pleural invasion and to determine whether visceral pleural invasion and bronchovascular bundle thickening on the same lobe could be related to the recurrence and survival in non-small cell lung cancer (NSCLC) Method: Eighty one patients, which were fulfilled long-term follow-up at least 18 months (maximum 103 months) among which 434 patients had underwent curative surgical resection for NSCLC from 1986 to 1995, were studied. They were analyzed to evaluate whether the prognostic factors such as the recurrence and survival depend on visceral pleural invasion and bronchovascular bundle thickening to the same lobe. Thirty two patients adjacent to a chest wall or a fissure were evaluated for visceral pleural invasion by HRCT. CT criteria included abutting pleura along the chest wall, abutting and/or compressing fissure, croosing fissure, and pleural tail. Results: The positive predictive value and the negative predictive value of crossing fissure were 100% and 100%, respectively. Two patients showing spiculated interface between a mass and abutting fissure were confirmed to have visceral pleural invasion at surgery. Visceral pleural invasion confirmed at surgery was significant to local recurrence and survival (p<.05. p<.05, respectively). Brochovascular bundle thickening to the same lobe on CT scan was significant to survival (p<.05) but was not significant to local and distant recurrence (p>.05). Conclusion : Visceral pleural invasion and bronchovascular bundle thickening to the same lobe have a role in predicting prognosis such as recurrence and survival in NSCLC. Therefore, the analysis of visceral pleural invasion on CT scan and the pathological analysis of bronchovascular bundle thickening to the same lobe may be necessary to predict the prognosis in NSCLC.