• Title/Summary/Keyword: Cell membranes

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Two Oxytrichid Ciliates, Cyrtohymena primicirrata and Oxytricha granulifera (Ciliophora: Sporadotrichida: Oxytrichidae) Unknown from Korea

  • Kwon, Choon Bong;Shin, Mann Kyoon
    • Animal Systematics, Evolution and Diversity
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    • v.29 no.1
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    • pp.23-30
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    • 2013
  • This study reports the discovery of two oxytrichid ciliates, Cyrtohymena primicirrata (Berger and Foissner, 1987) and Oxytricha granulifera Foissner and Adam, 1983, in Jeju Island, Korea. The morphology of the two species was studied using live observation and protargol impregnation. These species are described as follows: Cyrtohymena primicirrata has a body size in live specimens $90-140{\times}40-60{\mu}m$, length : width ratio 2.3 : 1 on average; elongated and slender obovate in outline of body. Cortical granules are shiny yellow on the ventral and dorsal side. Adoral zone of membranelles (AZM) is covering about 48% of the cell with about 38 adoral membranelles. Arrangement of undulating membranes is ordinary Cyrtohymena pattern. Dorsal kineties is six rows with $5{\mu}m$ long bristles. Oxytricha granulifera has a body size in live specimens $90-115{\times}25-38{\mu}m$, length : width ratio 3.31 on average; elongated ellipsoidal in outline of body. Cortical granules are colorless on the ventral and dorsal side. AZM is covering 28% of the cell length in vivo with about 24 adoral membranelles. Arrangement of undulating membranes is Oxytricha pattern. Dorsal kineties is five rows with about $3{\mu}m$ long dorsal bristles.

Ultrastructural Changes of Oocyte in Korean Catfish, Silurus asotus (발달 단계에 따른 한국산 메기(Silurus asotus) 난모세포의 미세구조적 변화)

  • 윤종만;김계웅;신호철;장계남;류동석;박홍양
    • Korean Journal of Animal Reproduction
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    • v.19 no.2
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    • pp.105-117
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    • 1995
  • This study was carried out to investigate the histomorphological changes and the electrophoretic patterns of egg components, obtained from 100 of 1-year-old female catfish(Silurus asotus). Especially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were observed by light and transmission electron microscope. All data were collected from October in 1992 to May in 1993. The size of the nucleoli and number of the yolk granules increased as the oocyte grew. Yolk granules were deposited in the oocyte as fluid. Due to the presence of large early and late maturing oocytes, their ovaries were large, transparent, granular, and greenish in color. As the percentages of fish in LMO and RO stage increased from March to April, mean of GSI values(19.95%) increased. Follicle cells such as granulosa cell and thecal cell change a squamous into cuboid shape in LPO and EMO stage. Processes, microvilli, from the granulosa cells and from the oocyte grow and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radiate becomes more compact and devoid of pore canals during oocyte maturation. The electrophoretic pattern of major band in mature stage was much thicker(21k, 24k, 32k, 45k, 67∼110k, 170k dalton) than that in previtellogenic phase.

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Characterization of Nafion/Pt/Polypyrrole Composite Membrane Prepared by Chemical In-situ Polymerization for DMFC (화학적 합성에 의해 제조된 직접 메탄올 연료전지용 나피온/백금/폴리피롤 복합 막의 특성 분석)

  • Park, Ho-Seok;Kim, Yo-Jin;Im, Hun-Suk;Choi, Bong-Gill;Hong, Won-Hi
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.11a
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    • pp.421-424
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    • 2006
  • Nafion/Pt/Polypyrrole composite membranes were fabricated by chemical in-situ polymerization of pyrrole monomers with Pt precursors in Nafion matrix for DMFC. We demonstrated that positively charged pyrrolinum groups of polypyrrole particles were co-interacted with sulfonic groups of Nafion as verified by FT-IR results. Mutual interaction between $Nafion-SO_3^-$ (or negatively charged Pt precursors) and Polypyrrole$-NH_2^+$ influenced the physical properties of pristine Nafion. Thermal property proton conductivity, methanol permeability, and cell performance of pristine and modified Nafion were analyzed for an application of DMFC membrane. Thermal stabilities of sulfonic groups and side chains in Nafion/Pt/polypyrrole composite membranes were higher than those of Nafion due to mutual interaction between sulfonic groups of Nafion and pyrrolinum groups of polypyrrole. Methanol permeabilities of Nafion/Pt/Polypyrrole composite were reduced more proton conductivities with the increase in the content of Pt particles. As a result of that, the enhancement of cell performance by Nafion/Pt/Polypyrole O2 relative to Nafion was more pronounced under the specific experimental condition such as high temperature and more concentrated methanol solution.

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Recovery of Useful Components from Rice-washing Water Using Membranes (분리막을 이용한 쌀뜨물내 유효성분의 회수)

  • 정건용;박성희
    • Membrane Journal
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    • v.12 no.3
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    • pp.165-170
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    • 2002
  • Membrane process was investigated to recover useful components, such as protein from rice-washing water generated in the production of the washed-rice. The filtration experiments were carried out using not only a dead-end Amicon cell to determine suitable membranes but also a hollow fiber ultrafiltration, spiral wound nanofiltration and reverse osmosis modules for home water purification. Ultrafiltration module(molecular weight cut-off : 10,000 dalton) was not suitable for recovery of useful components or protein in the rice-washing water, but nanofiltration and reverse osmosis modules showed a good performance. in the case of 250% concentration of the rice-washing water contained about 9% protein the proteins in concentrates of nanofiltration and reverse osmosis were 18% and 22%, which were about 2 and 2.4 times higher protein concentrations than those of feed, respectively.

Influence of Total Saponin from Korean Red Ginseng on Structural Changes in Phospholipid Membranes and Ghost Erythrocytes (고려홍삼의 총사포닌에 의한 인지질막과 적혈구막의 구조적 변화)

  • Kim, Yuri-A.;Vlasimir, R.Akoev;Tarahovsky, Yuri-S.;Ruslan, Elemesov;Park, Kyeong-Mee;Song, Yong-Bum;Rhee, Man-Hee;Park, Hwa-Jin
    • Journal of Ginseng Research
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    • v.19 no.1
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    • pp.39-44
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    • 1995
  • Total saponin from Korean red ginseng changed thermodynamic parameters of membranes from dipalmitoylphosphatidylcholine (DPPC) and ghost erythrocytes of human. In liposomes from DPPC, temperature of the main transition (Lb'-La) in liquid-crystalline phase increases by 0.2$^{\circ}C$ in average, but enthalpy does not change. Total saponin at a concentration of smaller than $10^5$% "stabilizes" the timid bilayers. At larger than 0.07 of saponin/DPPC ratio, saponin leads to an exclusion of the bound lipid molecules from the main phase transition into lamella liquid crystalline La-phase. Total saponin influences specifically all erythrocyte membrane transitions in a concentration-dependent manner, i.e. on the structures of all the main membrane skeleton proteins. A high structural specificity of saponin with membrane proteins, could be a base of specificity of physiological response of not only erythrocytes, but also other cells.her cells.

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Preparation and Characterization of Poly(styrenesulfonic acid)-grafted Fluoropolymer Membrane for Direct Methanol Fuel Cell

  • Choi, Jae-Hak;Kang, Phil-Hyun;Lim, Youn-Mook;Sohn, Joon-Yong;Shin, Jun-Hwa;Jung, Chan-Hee;Jeun, Joon-Pyo;Nho, Young-Chang
    • Korean Membrane Journal
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    • v.9 no.1
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    • pp.52-56
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    • 2007
  • A proton exchange membrane was prepared by ${\gamma}-irradiation-induced$ grafting of styrene into poly(tetrafluoro-ethylene-co-perfluoropropyl vinyl ether) (PFA) and subsequent sulfonation reaction. The degree of grafting (DOG) increased with an increase in the absorbed dose. The prepared membranes showed high ion exchange capacity reaching 3.0 meq/g, which exceeded the performance of commercially available perfluorosulfonic acid membranes such as Nafion. The proton conductivity of PFA-g-PSSA membrane increased with the DOG and reached 0.17 S/cm for the highest sample at room temperature. The DMFC performance of the prepared membranes with 50% DOG was comparable to that of Nafion membrane.

Poly(vinylbenzyl chloride-glycidyl methacrylate)/Polyethylene Composite Anion Exchange Membranes for Vanadium Redox Battery Application

  • Park, Min-A;Shim, Joonmok;Park, Se-Kook;Jeon, Jae-Deok;Jin, Chang-Soo;Lee, Ki Bong;Shin, Kyoung-Hee
    • Bulletin of the Korean Chemical Society
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    • v.34 no.6
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    • pp.1651-1655
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    • 2013
  • Anion exchange membranes for a vanadium redox flow battery (VRB) were prepared by pore-filling on a PE substrate with the copolymerization of vinylbenzyl chloride (VBC) and glycidyl methacrylate (GMA). The ion exchange capacity, water uptake and weight gain ratio were increased with a similar tendency up to 65% of GMA content, indicating that the monomer improved the pore-filling degree and membrane properties. The vanadium ion permeability and open-circuit voltage were also investigated. The permeability of the VG65 membrane was only $1.23{\times}10^{-7}\;cm^2\;min^{-1}$ compared to $17.9{\times}10^{-7}\;cm^2\;min^{-1}$ for Nafion 117 and $1.8{\times}10^{-7}\;cm^2\;min^{-1}$ for AMV. Consequently, a VRB single cell using the prepared membrane showed higher energy efficiency (over 80%) of up to 100 cycles compared to the commercial membranes, Nafion 117 (ca. 58%) and AMV (ca. 70%).

Asymmetrical Distribution of P2Y Nucleotide Receptors in Rabbit Inner Medullary Collecting Duct Cells

  • Woo, Jae-Suk;Jung, Jin-Sup;Kim, Yong-Keun
    • The Korean Journal of Physiology and Pharmacology
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    • v.4 no.4
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    • pp.311-318
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    • 2000
  • We cultured the rabbit inner medullary collecting duct (IMCD) cells as monolayers on collagen-coated membrane filters, and investigated distribution of the P2Y receptors by analyzing nucleotide-induced short circuit current $(I_{sc})$ responses. Exposure to different nucleotides of either the apical or basolateral surface of cell monolayers stimulated $I_{sc}.$ Dose-response relationship and cross-desensitization studies suggested that at least 3 distinct P2Y receptors are expressed asymmetrically on the apical and basolateral membranes. A $P2Y_2-like$ receptor, which responds to UTP and ATP, is expressed on both the apical and basolateral membranes. In addition, a uracil nucleotide receptor, which responds to UDP and UTP, but not ATP, is expressed predominantly on the apical membrane. In contrast, a $P2Y_1-like$ receptor, which responds to ADP and 2-methylthio-ATP, is expressed predominantly on the basolateral membrane. These nucleotides stimulated intracellular cAMP production with an asymmetrical profile, which was comparable to that in the stimulation of $I_{sc}.$ Our results suggest that the adenine and uracil nucleotides can interact with different P2Y nucleotide receptors that are expressed asymmetrically on the apical and basolateral membranes of the rabbit IMCD cells, and that both cAMP- and $Ca^{2+}-dependent$ signaling mechanisms underlie the stimulation of $I_{sc}$.

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Advances in Materials for Proton Exchange Membrane based Fuel Cells

  • McGrath James E.
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.58-59
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    • 2006
  • Less than a decade ago, most alternate membrane materials for fuel cells relied upon a post-sulfonation process to generate ionic groups capable of transporting protons from the anode to the cathode. These random post sulfonations showed some promise, but in general they produced materials that were not sufficiently stable or protonically conductive at ion exchange capacities where aqueous swelling could be restricted. Our group began to synthesize disulfonated monomers that could be used to incorporate into random copolymer proton exchange membranes. The expected limitation was that the aromatic polymers might not be stable enough to withstand fuel cell conditions. However, this was mostly based upon an accelerated test known was the Fenton's Reagent Test, which did not seem to this author as being a reliable predictor of performance. A much better approach has been to evaluate the open circuit voltage (OCV) for alternate membranes, as well as the benchmark perfluorosulfonic acid systems. When this is done, the aromatic ionomers of this study, primarily based upon disulfonated polyarylene ether sulfones, show up quite well. Real time 3000 hours DMFC results have also been generated. Obtaining conductive materials at low humidities is another major issue where alternate membranes have not been particularly successful. In order to address this problem, multiblock copolymers with relatively high water diffusion coefficients have been designed, which show promise for conductivity at lowered humidity.

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The effect of backing layer for pro membranes and modules (PRO 분리막 및 모듈성능에 지지체가 미치는 영향)

  • Han, Man Jae;Jeon, Eun Joo;Sim, Yeon-Ju;Lee, Jong Hwa
    • Journal of Korean Society of Water and Wastewater
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    • v.30 no.5
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    • pp.553-559
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    • 2016
  • There has been increasing global interest in the environmental pollution problems produced by fossil fuel consumption and greenhouse gas emissions. In order to tackle these issues, new renewable energy such as solar, wind, bio gas, fuel cell and pressure retarded osmosis(PRO) have been developed extensively. Among these energy sources, PRO is one of the salinity gradient power generation methods. In PRO, energy is obtained by the osmotic pressure generated from the concentration difference between high and low concentration solutions separated by a semipermeable membrane. The development for high power density PRO membranes is imperative with the purpose of commercialization. This study investigates development of thin film composite PRO membrane and spiral wound module for high power density. Also, the influence of membrane backing layer on power density was identified, and the characteristic factors of PRO membranes was determined. Different backing layers were used to improve power density. As expected, the PRO membrane with more porous backing layer showed higher power density.