• Archives of Reconstructive Microsurgery
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• v.18 no.1
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• pp.31-34
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• 2009

Purpose: Merkel cell carcinoma, also called neuroendocrine carcinoma, is a very rare type of skin cancer that develops as Merkel cells grow out of control. Merkel cell carcinoma is reported below 1% of whole skin neoplasms in the United States and is known that the 2-year survival rate is about 50~70%. The principles of treatment are wide excision of primary lesion with radiotherapy and/or chemotherapy that decrease the local recurrent rate. There has been no report of reconstruction with free flap after resection of Merkel cell carcinoma in Korea. Methods: We reconstructed the skin and soft tissue defect after wide excision of Merkel cell carcinoma with anterolateral thigh perforator free flap in two cases. No distant metastasis was found at the preoperative imaging work-up. In one case, preoperative chemotherapy was performed and the size of lesion was decreased. Results: There were no recurrence and significant complications. Functionally and aesthetically satisfactory results were obtained with reconstruction. Conclusion: Wide excision and reconstruction with anterolateral thigh perforator free flap for Merkel cell carcinoma patient is the first report in Korea. We regard this method as the treatment of choice in Merkel cell carcinoma.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.223-223
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• 2004

Nucleoside diphosphate kinases(NDPKs) are ubiquitous enzymes involved in numerous regulatory processes associated with transcriptional regulation, cell proliferation, development, and differentiation. In this study, we was examined characterization and localization of the nm23-M5 in mouse testis by Western blotting, immunohistochemical and conforcal imaging study using specific antibodies raised against nm23-M5. (omitted)

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2003.07a
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• pp.421-426
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• 2003

The Immunosensor based on antigen-antibody binding have been developed for detecting several analytes including antigen, small molecules, and cell. This method can be rapid and show very good detection limits. For Implementation of immunosensor, technologies for immobilization of antibody onto solid surface and detection of protein-protein binding must be developed. (an ellipsis)

• Applied Microscopy
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• v.47 no.4
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• pp.226-232
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• 2017

Electron microscopy and X-ray diffraction have together played a key role in our understanding of the molecular structure and mechanism of contraction of muscle. This review highlights the role of electron microscopy, from early insights into thick and thin filament structure by negative staining, to studies of single myosin molecule structure, and finally to recent high-resolution structures by cryo-electron microscopy. Muscle filaments are designed for movement. Their labile structures thus present challenges to obtaining near-atomic detail, which are also discussed.

• Proceedings of the Optical Society of Korea Conference
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• 2008.07a
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• pp.273-274
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• 2008

We present a quantitative phase microscopy for live cells. This method uses the principles of common path inteferometry and single shot phase image. This system has the ability to measure live cells quantitatively with subnanometer path length stability and millisecond scale aquisition time.

• Proceedings of the Optical Society of Korea Conference
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• 2008.07a
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• pp.381-382
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• 2008

• Investigative Magnetic Resonance Imaging
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• v.22 no.4
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• pp.240-244
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• 2018

Primary extranodal bone lymphoma involving the peripheral extremities is extremely rare. Here, we report a definitive case of diffuse large B-cell lymphoma involving the phalangeal bone of the 3rd finger. Systemic evaluation revealed the lesion as the only site of lymphoma involvement.

• Korean Journal of Radiology
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• v.23 no.4
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• pp.446-454
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• 2022

Objective: To evaluate whether hyperoxia-induced ΔR₁ (hyperO₂ΔR₁) can accurately identify histological infarction in an acute cerebral stroke model. Materials and Methods: In 18 rats, MRI parameters, including hyperO₂ΔR₁, apparent diffusion coefficient (ADC), cerebral blood flow and volume, and ¹⁸F-fluorodeoxyglucose uptake on PET were measured 2.5, 4.5, and 6.5 hours after a 60-minutes occlusion of the right middle cerebral artery. Histological examination of the brain was performed immediately following the imaging studies. MRI and PET images were co-registered with digitized histological images. The ipsilateral hemisphere was divided into histological infarct (histological cell death), non-infarct ischemic (no cell death but ADC decrease), and nonischemic (no cell death or ADC decrease) areas for comparisons of imaging parameters. The levels of hyperO₂ΔR₁ and ADC were measured voxel-wise from the infarct core to the non-ischemic region. The correlation between areas of hyperO₂ΔR₁-derived infarction and histological cell death was evaluated. Results: HyperO₂ΔR₁ increased only in the infarct area (p ≤ 0.046) compared to the other areas. ADC decreased stepwise from non-ischemic to infarct areas (p = 0.002 at all time points). The other parameters did not show consistent differences among the three areas across the three time points. HyperO₂ΔR₁ sharply declined from the core to the border of the infarct areas, whereas there was no change within the non-infarct areas. A hyperO₂ΔR₁ value of 0.04 s^-1 was considered the criterion to identify histological infarction. ADC increased gradually from the infarct core to the periphery, without a pronounced difference at the border between the infarct and non-infarct areas. Areas of hyperO₂ΔR₁ higher than 0.04 s^-1 on MRI were strongly positively correlated with histological cell death (r = 0.862; p < 0.001). Conclusion: HyperO₂ΔR₁ may be used as an accurate and early (2.5 hours after onset) indicator of histological infarction in acute stroke.

• Journal of Korea Foresty Energy
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• v.20 no.1
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• pp.62-72
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• 2001

To elucidate the behaviors of alkali swelllng of woods. the dimensional change in cross section of cell elements of four major Korean woods during alkali swelling were examined by an optical microscory, an imaging analysis method and an X-ray diffrartion During alkali swelling, tracheid diameter of Larix kaempferi wood showed greater swelling property than that of Pinus koraiensis wood, and the cell wall swelled highly over 10% sodium hydroxide solution treatment. The radial diameter of vessel elements in earlywood shrunk, but it swelled slightly in tangential direction. When treated with 5% NaOH, the wall thickness of wood fiber increased about three times over the original one. The thickness of cell wall in all elements and the diameter of wood fiber and tracheid showed almost isotropic shrinkage. The diameter of cell elements during the mercerization process decreased, but cell wall thickness Increased. Crystal transformation of cellulose in wood was not occurred by alkali treatments. but relative crystallinity and crystallite width of the woods increased slightly. Consequently, it was demonstrated that the swelling properties of woods were dependant on wood species, cell elements and alkali concentration.

• Transactions of the Korean hydrogen and new energy society
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• v.28 no.5
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• pp.521-530
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• 2017

Improving the microstructure of NiO/YSZ is one of several approaches used to enhance the electrical and mechanical properties of an anode support in Solid Oxide Fuel Cells (SOFCs). The aim of the work reported in this paper was to predict the relationship between these microstructural changes and the resulting properties. To this end, modification of the anode microstructure was carried out using different sizes of Poly (Methyl Methacrylate) (PMMA) beads as a pore former. The electrical conductivity and mechanical strength of these samples were measured using four-probe DC, and three-point bend-test methods, respectively. Thermal etching followed by high resolution SEM imaging was performed for sintered samples to distinguish between the three phases (NiO, YSZ, and pores). Recently developed image analysis techniques were modified and used to calculate the porosity and the contiguity of different phases of the anode support. Image analysis results were verified by comparison with the porosity values determined from mercury porosimetry measurements. Contiguity of the three phases was then compared with data from electrical and mechanical measurements. A linear relationship was obtained between the contiguity data determined from image analysis, and the electrical and mechanical properties found experimentally. Based upon these relationships we can predict the electrical and mechanical properties of SOFC support from the SEM images.