• Asian Pacific Journal of Cancer Prevention
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• 제15권7호
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• pp.3211-3217
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• 2014

Background: The current study investigated the effects of Piper longumine on radio-sensitization of human breast cancer MDA-MB-231 cells and underlying mechanisms. Materials and Methods: Human breast cancer MDA-MB-231 cells were cultured in vitro and those in logarithmic growth phase were selected for experiments divided into four groups: control, X-ray exposed, Piper longumine, and Piper longumine combined with X-rays. Conogenic assays were performed to determine the radio-sensitizing effects. Cell survival curves were fitted by single-hit multi-target model and then the survival fraction (SF), average lethal dose ($D_0$), quasi-threshold dose ($D_q$) and sensitive enhancement ratio (SER) were calculated. Cell apoptosis was analyzed by flow cytometry (FCM). Western blot assays were employed for expression of apoptosis-related proteins (Bc1-2 and Bax) after treatment with Piper longumine and/or X-ray radiation. The intracellular reactive oxygen species (ROS) level was detected by FCM with a DCFH-DA probe. Results: The cloning formation capacity was decreased in the group of piperlongumine plus radiation, which displayed the values of SF2, D0, Dq significantly lower than those of radiation alone group and the sensitive enhancement ratio (SER) of D0 was1.22 and 1.29, respectively. The cell apoptosis rate was increased by the combination treatment of Piper longumine and radiation. Piper longumine increased the radiation-induced intracellular levels of ROS. Compared with the control group and individual group, the combination group demonstrated significantly decreased expression of Bcl-2 with increased Bax. Conclusions: Piper longumine at a non-cytotoxic concentration can enhance the radio-sensitivity of MDA-MB-231cells, which may be related to its regulation of apoptosis-related protein expression and the increase of intracellular ROS level, thus increasing radiation-induced apoptosis.

• 대한영상의학회지
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• 제84권6호
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• pp.1391-1396
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• 2023

악성 림프종은 흉부 CT에서 결절, 종양, 폐 경화, 간유리음영 등 다양한 폐질환으로 나타날 수 있으며 이러한 폐 병변은 다른 질환의 양상과 유사하여 진단에 어려움을 줄 수 있다. 본 증례는 호흡곤란과 발열을 주 증상으로 하는 67세 남성 환자로 해당 환자의 흉부 CT상 크기가 작은 다발성 결절이 양측에 미만성으로 나타나 혈행성 전이, 좁쌀 결핵 또는 진균 감염을 의심해 볼 수 있었다. 그러나 추가적으로 시행한 혈액 검사, 영상 검사 및 조직 생검 결과 폐를 침범한 미만성 큰 B세포 림프종이 진단되었다. 저자들은 폐의 좁쌀 결절로 발현한 매우 드문 림프종을 보고한다. 환자의 적절한 진단을 위해서는 환자의 병력, 신체 진찰, 혈액 검사 및 영상 소견의 포괄적인 평가가 필요하다.

• 한국임상수의학회지
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• 제32권4호
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• pp.301-307
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• 2015

본 연구의 목적은 적색 형광 단백질 유전자 형질 전환 복제견을 포함한 복제견들에서 측정된 심장 초음파 수치를 이전에 보고된 일반개의 정상 참고 범위와 비교하는 것이다. 일곱 마리의 복제견에서 M-mode, 2D-mode, pulsed wave Doppler 그리고 Tissue Doppler Imaging을 통해 좌심, 우심 그리고 우심실 우출로의 해부학적 특징과 심장 기능을 평가하였다. 모든 복제견들은 해부학적 구조에서 특이적인 이상을 나타내지 않았으며 측정된 수치들은 정상 참고 범위 이내에 있었다. 게다가 좌심과 우심의 심근 기능 모두 정상 참고 범위 이내에 위치하였다. 특히 복제 동물들에서 흔히 나타나는 우심 부전과 폐성 고혈압은 복제견들에서는 나타나지 않았다. 결론적으로, 성장이 끝난 복제견들에게서 는 출생시와 성장과정에서 심혈관계의 이상을 타나내는 징후는 확인되지 않았다. 그러므로 형질 전환 방법을 포함하는 체세포핵이식 기술은 복제견들의 심장 형태와 기능에 대해 심각한 부작용을 나타내지 않는다고 할 수 있다.

• Imaging Science in Dentistry
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• 제52권1호
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• pp.109-115
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• 2022

Purpose: The aim of this study was to evaluate the prevalence of clinically relevant anatomical variations of the ethmoid sinuses and their potential association with ethmoid and maxillary sinus pathologies on cone-beam computed tomography (CBCT) scans. Additionally, potential associations with different sides and demographic factors, including age and sex, were evaluated. Materials and Methods: In total, 273 CBCT scans with complete ethmoid and maxillary sinuses were analyzed to determine the prevalence of Agger nasi cell, supraorbital ethmoid cell, Haller cell, Onodi cell, and ethmomaxillary sinus. In addition, the health or pathology of the ethmoid and maxillary sinuses was also recorded to assess correlations with the aforementioned variations. Results: The prevalence of Agger nasi cell was found to be the highest (95.6%) in this study, followed by Onodi cell (60.4%), Haller cell (29.3%), and supraorbital ethmoid cell (19.4%). Ethmomaxillary sinus was the least common finding (16.5%). Males and persons above 61 years of age had a significantly higher frequency of supraorbital ethmoid cell and Onodi cell, respectively. However, no significant relationships were noted between anatomical variations of the ethmoid sinus and pathologies of the ethmoid or maxillary sinus. Conclusion: There was a high prevalence of ethmoid sinus variations in this Southern Chinese population. The prevalence of Agger nasi cell and Onodi cell was higher than that of other anatomical variations of the ethmoid sinuses. Anatomical variations of the ethmoid sinuses were not associated with ethmoid or maxillary sinus pathologies in this patient cohort.

• 신재생에너지
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• 제3권2호
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• pp.24-30
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• 2007

The transient response of PEMFC (proton exchange membrane fuel cell) is important criteria in the application of PEM fuel cell to real automotive system. In this work, using a transparent unit PEM fuel cell, the transient response and cathode flooding during load change are investigated. The cell voltage is acquired according to the current density change($0.3Acm^2$ to $0.6A/cm^2$) under various stoichiometry conditions and different flooding intensities, Also the cathode gas channel images are obtained by CCD imaging system simultaneously. The different level of undershoots appeared at the moment of load changes under different cathode stoichiometries and flooding intensities. It takes about 1s that the product water comes up onto the flow channel so that oxygen supply is temporarily blocked which causes voltage loss in that "undershoot". The correlation of the dynamic behavior with stoichiometry and cathode flooding is induced from the results of these experiments.

• Applied Microscopy
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• 제47권3호
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• pp.101-104
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• 2017

In this investigation, synchrotron X-ray imaging was used to investigate the water distribution inside newly developed gas diffusion media in polymer electrolyte membrane fuel cells. In-situ radiography was used to reveal the relationship between the structure of the microporous layer (MPL) and the water flow in a newly developed MPL equipped with randomly arranged holes. A strong influence of these holes on the overall water transport was found. This contribution provides a brief overview to some of our recent activities on this research field.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.462-467
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• 2003

For the Space-borne optical imaging payload system design, light weighting and moderate stiffness of mirror and/or mirror fixation device is very important aspects. The front surface of mirror is regulated by optical performance requirement, but the shape of backplate of mirror is to be optimised while satisfing the required stiffness and weight. According to the results, the best shape of backplate cell is triangular. And also related geometric dimensions and the optimised mounting point of MFD(Mirror Fixation Device) is presented. Finally, natural frequencies and shpaes of mirror structure are analysed.

• BMB Reports
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• 제46권2호
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• pp.65-72
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• 2013

In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demon-strating the importance of coding and noncoding RNA transcripts in these processes. However, these techniques typically provide ensemble averages of transcription across many cells. In situ hybridization-based analysis methods complement these studies by providing information about how expression levels change between cells within normal and diseased tissues, and they provide information about the localization of transcripts within cells, which is important in understanding mechanisms of gene regulation. Multi-color, single-molecule fluorescence in situ hybridization (smFISH) is particularly useful since it enables analysis of several different transcripts simultaneously. Combining smFISH with immunofluorescent protein detection provides additional information about the association between transcription level, cellular localization, and protein expression in individual cells.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 추계학술대회 논문집
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• pp.14-16
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• 2008

자동차 구동용 연료전지 스택에 적용된 분리판에 대하여 연료전지 내부의 수분분포 및 농도를 측정할 수 있는 중성자 가시화 기법을 이용하여 구조진단을 실시하여 유로의 분기부 및 180도 회전부의 수분 응축과 같은 국부적인 Flooding 현상과 분리판의 반응면적 전체에 대한 불균일한 수분분포를 확인하였다. 신규 개발 스택에 적용된 분리판은 이러한 구조진단 결과를 바탕으로 변형된 유로 도입을 통한 180도 회전부 제거, 냉각수 입구와 인접한 부분에서 교차하게 되는 수소 출구 부분의 수분응축에 의한 Flooding 현상을 완화하기 위한 냉각수 유로를 적용하여 중성자 가시화 기법을 통하여 동일한 가습조건에서 부하에 따른 분리판 반응면적 전체에 대한 수분분포를 조사하였다.

• Molecules and Cells
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• 제41권9호
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• pp.809-817
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• 2018

Discovery of the naturally evolved fluorescent proteins and their genetically engineered biosensors have enormously contributed to current bio-imaging techniques. These reporters to trace dynamic changes of intracellular protein activities have continuously transformed according to the various demands in biological studies. Along with that, light-inducible optogenetic technologies have offered scientists to perturb, control and analyze the function of intracellular machineries in spatiotemporal manner. In this review, we present an overview of the molecular strategies that have been exploited for producing genetically encoded protein reporters and various optogenetic modules. Finally, in particular, we discuss the current efforts for combined use of these reporters and optogenetic modules as a powerful tactic for the control and imaging of signaling events in cells and tissues.