• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.739-748
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• 2001

A self-organizing fuzzy logic controller using a genetic algorithm is described, which controlled the glucose concentration for the enhancement of flavonoid production in a fed-batch cultivation of Scutellaria baicalensis G. plant cells. The substrate feeding strategy in a fed-batch culture was to increase the flavonoid production by using the proposed kinetic model. For the two-stage culture, the substrate feeding strategy consisted of a first period with 28 g/I of glucose to promote cell growth, followed by a second period with 5 g/I of glucose to promote flavonoid production. A simple fuzzy logic controller and the self-organizing fuzzy logic controller using a genetic algorithm was constructed to control the glucose concentration in a fed-batch culture. The designed fuzzy logic controllers were applied to maintain the glucose concentration at given set-points of the two-stage culture in fed-batch cultivation. The experimental results showed that the self-organizing fuzzy logic controller improved the controller\`s performance, compared with that of the simple fuzzy logic controller. The specific production yield and productivity of flavonoids in the two-stage culture were higher than those in the batch culture.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.19 no.12
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• pp.1149-1153
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• 2006

We developed the equipment palpating cell proliferation using a high brightness LED. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity, frequency and so on. Especially, to control the light irradiation frequency, FPGA was used, and to control the change of output value, TLC5941 was used. Control stage is divided into 30 levels by program. Consequently, the current value could be controlled by the change of level in Continue Wave(CW) and Pulse Width Modulation(PWM), and the output of a high brightness LED could be controlled stage by stage.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.115-118
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• 2000

A microfabricated cell chip was developed to evaluate drug effect on mouse B16-F1 melanoma cell line. The cell chip system consists of 8-well culture cartridge incorporated with interdigitated array gold electrodes on each well, lock-in amplifier, 8-well cell scanner and computer as a system controller. Impedance of an electrode is increased according to adherent cell growth on the electrode surface. In order to verify investigated. The change of impedance was measured differentially between a control electrode containing only media and cell-cultured sample electrodes with time.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.06a
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• pp.512-513
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• 2008

The study examined what effects 100kHz PWM LED light irradiation causes to bone marrow cells of SD-Rat when LED characterized cheap and safe is used onto the light therapy by replacing the low 1evel laser. We developed the equipment palpating cell proliferation using a high brightness LED. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity, frequency and so on. Especially, to control the light irradiation frequency, FPGA was used, and to control the change of output value, TLC5941 was used. Consequent1y, the current value could be controlled by the change of 1eve1 in Continue Wave(CW) and Pulse Width Modulation(PWM), and the output of a high brightness LED could be controlled stage by stage. MTT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring 590nm transmittance of ELISA reader. As a result, the cell increase of Rat bone marrow cells was verified in 100kHz PWM LED light irradiation group as compared to non-irradiation group.

• Fashion & Textile Research Journal
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• v.16 no.3
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• pp.446-455
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• 2014

This study revealed the results related to the functionality of outdoor wear required when man and women in their 30s to 50s engage in outdoor activities. Based on the results of the survey, we proposed composition draft for a smart outdoor jacket with main functions of GPS device and light-emitting device using solar cell and EL. Absorption and release of sweat, functionality regarding rain and wind, and lightweightness, etc. from material functionalities of outdoor wear were found to be important. From function required in clothing for outdoor activity, location tracking, night glow, and lighting functions were found to be most important. For results investigating the necessity of the jacket's location tracking function and lighting function using solar cell, high scores of 3.9~4.0 were given. Purchase intentions for smart outdoor jacket with location tracking and lighting functions devised by this study were fairly positive and most responses indicated that the appropriate purchase price was between 200,000 to 300,000 won while possible problems of this smart outdoor jacket were listed in the order of washing inconvenience, high price, device weight, and discomfort in movement. The draft proposal to integrate with wearable devices for smart outdoor jacket prototype is as follows: Solar cell has been attached to the upper arm but placed inside a transparent pocket which has been detachable for washing convenience while the solar cell and controller have been integrated into a single unit. Using frequent movement exhibited by the arms, EL has been attached along the center line of the raglan sleeve for easy spotting when used as an emergency signal or for night lighting function during outdoor activity. GPS has been attached on the left sleeve so that the person can bend the left arm inward and operate the GPS screen with the right hand while walking or running outdoors.

• Microbiology and Biotechnology Letters
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• v.21 no.4
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• pp.354-365
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• 1993

A novel control method with automatic tuning of PID controller parameters has been developed for efficient regulation of dissolved oxygen concentration in fed-batch fermentations of Escherichia coli. Agitation speed and oxygen partial pressure in the inlet gas stream were chosen to be the manipulated variables. A heuristic reasoning allowed improved tuning decisions from the supervision of control performance indices and it coule obviate the needs for process assumptions or disturbance patterns. The control input consisted of feedback and feedforword parts. The feedback part was determined by PID control and the feedforward part is determined from the feed rate. The proportional gain was updated on-line by a set of heuristics rules based on the supervision of three performance indices. These indices were output error covariance, the average value of output error, and input covariance, which were calculated on-line using a moving window. The integral and derivative time constants were determined from the period of output response. The specific growth rate was maintained at a low level to avoid acetic acid accumulation and thus to achieve a high cell density. The specific growthe rate was estimated from the carbon dioxide evolution rate. In fed-batch fermentation, the simutaneous control of dissolved oxygen concentration (at 0.2; fraction of saturated value) and specific growth rate (at 0.25$hr^{-1}$) was satisfactory for the entire culture period in spite of the changes in the feed rate and the switching of control input.

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.263-269
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• 2019

In this study, the characteristics and operation of a multi-purpose loader equipped with various sensors such as a sensor capable of measuring the boom length, an angle sensor capable of measuring the tilt of the left and right sides of the boom and the loader, and a load cell capable of measuring the weight during lifting We have implemented a system that displays related data values. The configuration of the system reads the values from the sensors, sends them to the vehicle controller, and transmits the calculated results of the overturn rate and other important information to the display device using the CANOpen protocol. Also, in the calculation of the overturn ratio, the structure of the multi-purpose loader is similar to that of the crane belonging to the heavy equipment, and the crane overturn rate calculation method is used. Through this study, we can observe the condition of the heavy equipment and recognize the emergency situations such as abalone through the display device.