• Biomolecules & Therapeutics
• /
• v.27 no.1
• /
• pp.78-84
• /
• 2019

Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5' UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.7 no.2
• /
• pp.171-177
• /
• 2002

The Photovoltaic systems with solar cell way Provide electrical energy to the utility/consumers, which are becoming one of the promising energy substitutes. The photovoltaic system can be classified into two types : One is the stand-alone type, and the other utility interactive one. The latter can return the generated power to the utility, but the former can't. The utility interactive systems are so valuable for peak power cut in summer season. In the photovoltaic systems the maximum power point tracking (MPPT) has been studied for the increase of the generating energy of the photovoltaic system. There are many control methods of MPPT, but a new MPPT algorithm is proposed to overcome the disadvantages of the conventional ones, and as a result the proposed method enables to improve both tracking ability and generating efficiency of photo voltaic system without DC chopper.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.14 no.10
• /
• pp.5310-5316
• /
• 2013

Maximum Power Point Tracking (MPPT) method for Photovoltaic (PV) system is implemented in PV inveter and it generate the maximum electric power from PV cell. MPPT method has been studied to have high efficiency and high tracking speed. However, these studies are basically focused on the performance under fixed irradiance condition. Based on the typical Perturbation and Observation (P&O) method, this paper presents the research results on modified P&O method to have a better performance under varying irradiance condition. The modified P&O method can have a better performance under varying irradiance condition because the additional measurements during the MPPT control period are conducted. The proposed MPPT method is verified by using 250kW PV inverter under linearly varying irradiance condition according to EN 50530.

• Biomolecules & Therapeutics
• /
• v.30 no.2
• /
• pp.170-178
• /
• 2022

The airway epithelium is equipped with the ability to resist respiratory disease development and airway damage, including the migration of airway epithelial cells and the activation of TLR3, which recognizes double-stranded (ds) RNA. Primary cilia on airway epithelial cells are involved in the cell cycle and cell differentiation and repair. In this study, we used Beas-2B human bronchial epithelial cells to investigate the effects of the TLR3 agonist polyinosinic:polycytidylic acid [Poly(I:C)] on airway cell migration and primary cilia (PC) formation. PC formation increased in cells incubated under serum deprivation. Migration was faster in Beas-2B cells pretreated with Poly(I:C) than in control cells, as judged by a wound healing assay, single-cell path tracking, and a Transwell migration assay. No changes in cell migration were observed when the cells were incubated in conditioned medium from Poly(I:C)-treated cells. PC formation was enhanced by Poly(I:C) treatment, but was reduced when the cells were exposed to the ciliogenesis inhibitor ciliobrevin A (CilioA). The inhibition of Beas-2B cell migration by CilioA was also assessed and a slight decrease in ciliogenesis was detected in SARS-CoV-2 spike protein (SP)-treated Beas-2B cells overexpressing ACE2 compared to control cells. Cell migration was decreased by SP but restored by Poly(I:C) treatment. Taken together, our results demonstrate that impaired migration by SP-treated cells can be attenuated by Poly(I:C) treatment, thus increasing airway cell migration through the regulation of ciliogenesis.

• Clinical and Experimental Pediatrics
• /
• v.59 no.4
• /
• pp.190-195
• /
• 2016

Purpose: Severe aplastic anemia (SAA), a fatal disease, requires multiple transfusion, immunosuppressive therapy, and finally, hematopoietic stem cell transplantation (HSCT) as the definitive treatment. We hypothesized that iron overloading associated with multiple transfusions and HSCT-related complications may adversely affect cardiac function. Left ventricular (LV) function was assessed in children after HSCT for SAA. Methods: Forty-six consecutive patients with a median age of 9.8 years (range, 1.5-18 years), who received HSCT for SAA and who underwent comprehensive echocardiography before and after HSCT, were included in this study. The data of LV functional parameters obtained using conventional echocardiography, tissue Doppler imaging (TDI), and speckle-tracking echocardiography (STE) were collected from pre- and post-HSCT echocardiography. These data were compared to those of 40 age-matched normal controls. Results: In patients, the LV ejection fraction, shortening fraction, end-diastolic dimension, mitral early diastolic E velocity, TDI mitral septal E' velocity, and STE LV longitudinal systolic strain rate (SSR) decreased significantly after HSCT. Compared to normal controls, patients had significantly lower post-HSCT early diastolic E velocity and E/A ratio. On STE, patients had significantly decreased LV deformational parameters including LV longitudinal systolic strain (SS), SSR, and diastolic SR (DSR), and circumferential SS and DSR. Serum ferritin levels showed weak but significant correlations (P<0.05) with LV longitudinal SS and SSR and circumferential SS and DSR. Conclusion: Subclinical LV dysfunction is evident in patients after HSCT for SAA, and was associated with increased iron load. Serial monitoring of cardiac function is mandatory in this population.

• Journal of Institute of Control, Robotics and Systems
• /
• v.7 no.10
• /
• pp.827-836
• /
• 2001

The PID controller has been widely applied to the most control systems because of its simple structure and east designing. One of the important points to design the PID control system is to tune the approximate control parameters for the given target system. To find the PID parameters using Ziegler Nichols(ZN) method needs a lot of experience and experiments to ensure the optimal performance. In this paper, CMIA(Cell Mediated Immune Algorithm) controller is proposed to drive the autonomous guided vehicle (AGV) more effectively. The proposed controller is based on specific immune responses of the biological immune system which is the cell mediated immunity. To verify the performance of the proposed CMIA controller, some experiments for the control of steering and speed of that AGV are performed. The tracking error of the AGV is mainly investigated for this purpose. As a result, the capability of realization and reliableness are proved by comparing the response characteristics of the proposed CMIA controllers with those of the conventional PID and NNPID(Neural Network PID) controller.

• The Transactions of the Korean Institute of Electrical Engineers P
• /
• v.59 no.3
• /
• pp.293-297
• /
• 2010

Next generation concentrating photovoltaic technologies could have a large-scale impact on world electricity production once they will become economically attractive and grid parity will be reached. Multi-junction solar cells will be characterised by a high value of the cell economical performance index if the cells were able to operate at high concentration level. Concentrating the sunlight by optical devices like lenses or mirrors reduces the area of expensive solar cells or modules, and, moreover, increases their efficiency. Accurate and reliable tracking is an important issue to maintain high the CPV system output power. Further, for high concentration CPV systems, the actual tracker cost is about 20% of the total CPV system cost. In this paper high-concentration is defined as systems using concentration ratios well above 100 times the one sun intensity and trackerlss CPV system studied. Using sphericalness lens and parallel MJ cell connection method were suggested and achieved experiment on a clear day in summer. Development of these high performance multi-junction CPV module promises to accelerate growth in photovoltaic power generation.

• Journal of Electrical Engineering and Technology
• /
• v.9 no.5
• /
• pp.1454-1470
• /
• 2014

This paper propose a new power conditioner topology with intelligent power management controller that integrates multiple renewable energy sources such as solar energy, wind energy and fuel cell energy with battery backup to make best use of their operating characteristics and obtain better reliability than that could be obtained by single renewable energy based power supply. The proposed embedded controller is programmed for maintaining a constant voltage at PCC, maximum power point tracking for solar PV panel and WTG and power flow control by regulating the reference currents of the controller on instantaneous basis based on the power delivered by the sources and load demand. Instantaneous variation in reference currents of the controller enhances the controller response as it accommodates the effect of continuously varying solar insolation and wind speed in the power management. The power conditioner uses a battery bank with embedded controller based online SOC estimation and battery charging system to suitably sink or source the input power based on the load demand. The simulation results of the proposed power management system for a standalone solar/WTG/fuel cell fed hybrid power supply with real time solar radiation and wind velocity data collected from solar centre, KEC for a sporadically varying load demand is presented in this paper and the results are encouraging in reliability and stability perspective.

• Molecules and Cells
• /
• v.42 no.2
• /
• pp.104-112
• /
• 2019

Tracking the fate of individual cells and their progeny through lineage tracing has been widely used to investigate various biological processes including embryonic development, homeostatic tissue turnover, and stem cell function in regeneration and disease. Conventional lineage tracing involves the marking of cells either with dyes or nucleoside analogues or genetic marking with fluorescent and/or colorimetric protein reporters. Both are imaging-based approaches that have played a crucial role in the field of developmental biology as well as adult stem cell biology. However, imaging-based lineage tracing approaches are limited by their scalability and the lack of molecular information underlying fate transitions. Recently, computational biology approaches have been combined with diverse tracing methods to overcome these limitations and so provide high-order scalability and a wealth of molecular information. In this review, we will introduce such novel computational methods, starting from single-cell RNA sequencing-based lineage analysis to DNA barcoding or genetic scar analysis. These novel approaches are complementary to conventional imaging-based approaches and enable us to study the lineage relationships of numerous cell types during vertebrate, and in particular human, development and disease.

• Proceedings of the KIEE Conference
• /
• 2011.07a
• /
• pp.1390-1391
• /
• 2011

태양광 발전 시스템에서의 최대 전력점 추적(MPPT, Maximum Power Point Tracking)제어를 실험을 하기 위해 부분선형 다이오드 모델을 이용한 태블로 해석을 통해 태양광 발전 시스템의 태양전지 모듈의 특성을 시뮬레이션 하였다. 태양전지 모듈의 V-I 특성과 태양전지 패널을 직렬-병렬 어레이로 연결 시, 부분 그늘 문제(Partial Shading Problem)의 지역 최대 점(global peak)을 시뮬레이션을 통해 확인하였다.