• Journal of Periodontal and Implant Science
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• v.32 no.1
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• pp.13-23
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• 2002

The ultimate goal of periodontal therapy is directed to arresting the progression of the disease, and regenerating the fibrous attachment. In order to achieve such treatment aim, the plaque and calculus must be eliminated and the physiological conditions of the root surface must be changed to facilitate the attachment and migration of the new fibroblasts, The method of changing the proper root surface conditions to promote the healing of periodontal tissue involves mechanical procedures, such as scaling and root planing, and chemical procedures such as tetracycline-HCl. However, the formation of a long junctional epithelium was most frequently observed type of healing. Thus, the aim of this study was to examine in vitro the influence of surface conditioning of dentin by TC-HCl on human gingival epithelial cell attachment. Human gingival epithelial cells were obtained from healthy retromolar pad area(under the age 23 years). Seventy two teeth extracted from severe periodontitis were used as study material. To evaluate the epithelial cell attachment to dentin, the prepared specimen was divided to four groups. For the control group, only scaling and root planing were carried out, and for the test group, 1 to 3, the concentration of the TC-HCl was 50, 125 and 250mg/ml respectively. After cell cultivation time of 1-, 3-. 24 hour, for the indirect quantitative assessment of gingival epithelial cell attached to dentin sample, the absorbance of epithelial cell unattached to dentin was measured. The results were as follows; 1. There was no statistically significant difference between scaling and root planing group and TC-HCl 50mg/ml 125mg/ml and 250mg/ml group about absorbance of unattached epithelial cell to dentin sample(p>0.5). 2. As time passes, the absorbance of unattached gingival epithelial cell to dentin sample was decreased statistically significant(p<0.05). 3. There was no statistically significant difference among the TC-HCl group(p>0.05) We concluded that there was similar effect on gingival epithelial cell attachment between TC-HCl conditioning on root surface and only scaling and root planing treatment

• Journal of Periodontal and Implant Science
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• v.24 no.3
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• pp.581-596
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• 1994

This in vitro study was undertaken to obtain optimal tetracycline concentration that aids proliferation and spreading of human periodontal ligament cells, for clinical application in root surfaces of periodontally diseased teeth. Periodontal ligament cells used in this study were obtained from explants of periodontal ligament of 1st premolar teeth which were extracted for the purpose of orthodontic treatment. The cells were cultured in Dulbecco's Modified Eagle Medium(DMEM) supplemented with 100 U/ml penicillin, $100\;{\mu}g/ml$ streptomycin and 10% FBS at $37^{\circ}C$, 100% humidity, 5% $CO_2-95%$ air. Cells were used between the third to 4th passage. After root planing of periodontally extracted teeth, the root slabs were cut with carborundum disk. In the cell proliferation experiment, experimental groups were root planing only group, immersed groups in 25, 50, 75, 100, 150mg/ml aqueous solution of Tetracycline HCl followed by a vigorous rinse in PBS. Human PDL cells at concentration of $1{\times}10^5\;cells/ml$ were seeded in each culture well which contained root slabs and incubated for 6 hours. Then, all of the root slabs were moved into new 24 culture well and incubated 24, 48 and 72 hours. The cell counting was done by inverted phase contrast microscope after trypsinization. The following results were obtained. The cell number was increased in order root planing only group, 25, 150, 50, 75, 100mg/ml of Tetracycline HCl treated group in 24, 48 and 72 hours. The maximal cell number was obtained when the root slabs were immersed in solution with 100mg/ml of Tetracycline HCl. There were statistically significant between the root planing only group and 75, 100 mg/ml of Tetracycline HCl treated group in 24 hours, between the root planing only group and 100mg/ml of Tetracycline HCl treated group in 48 hours, between the root planing only group and 50, 75, 100mg/ml of Tetracycline HCl treated group, between 25 and 100mg/ml of Tetracycline HCl treated group in 72 hours(p<0.05). In the cell spreading experiment, after 30 minutes of incubated, in the root planing only group, the cells were generally round in shape. The cell surface was mostly covered with blebs. The cells started to attach to root surface by cytoplasmic extension in 50, 100mg/ml of Tetracycline HCl treated groups, more numerous cells attached to root surface than root planing only group. Many orifices of dentinal tubule were exposed, cells showed radially spreaded cytoplasm and unspreaded central region of the cell was covered with blebs. After 6 hours of incubation, in the root planing only group, cells showed radially spreaded cytoplasm and were attached flat appearance. In 50, 100mg/ml of Tetracycline HCl treated groups, cellular margin was concaved and cytoplasm showed elongated appearance with polarity. After 24 hours of incubation, in the root planing group, cells showed characteristic polarity. In 50, 100mg/ml of Tetracycline HCl treated groups, cells showed more elongated and spindle - like appearance.

• ETRI Journal
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• v.20 no.3
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• pp.251-271
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• 1998

It is well known that if usage parameter control/network parameter control (UPC/NPC) functions are used together with a cell loss priority control scheme in ATM networks, the measurement phasing problem can occur. This makes it difficult for a network provider to define and commit the cell loss ratio as a QoS parameter. To solve the problem, we propose a new UPC/NPC algorithm. By using the proposed UPC/NPC algorithm, we can define the cell loss ratios for CLP = 0 and CLP = 0+1 cell streams without the measurement phasing problem under any conditions. We analyzed the performance of the proposed UPC/NPC algorithm. Using a discrete time model for the UPC/NPC architecture with a discrete-time semi-Markov process (DSMP) input model, we obtained the cell discarding probabilities of CLP = 0 and CLP = 0+1 cells streams and showed that more CLP = 0 cells are accepted compared to what was proposed in ITU-T.

• Journal of Periodontal and Implant Science
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• v.40 no.6
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• pp.257-264
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• 2010

Purpose: Connective tissue reattachment to periodontally damaged root surfaces is one of the most important goals of periodontal therapy. The aim of this study was to develop a root conditioning agent that can demineralize and detoxify the infected root surface. Methods: Dentin slices obtained from human teeth were treated with a novel root planing agent for 2 minutes and then washed with phosphate-buffered saline. Smear layer removal and type I collagen exposure were observed by scanning electron microscopy (SEM) and type I collagen immunostaining, respectively. Cell attachment and lipopolysaccharides (LPS) removal demonstrated the efficiency of the root conditioning agent. Results: SEM revealed that the smear layer was entirely removed and the dentinal tubules were opened by the experimental gel. Type I collagen was exposed on the surfaces of the dentin slices treated by the experimental gel, which were compared with dentin treated with other root planing agents. Dentin slices treated with the experimental gel showed the highest number of attached fibroblasts and flattened cell morphology. The agar diffusion assay demonstrated that the experimental gel also has effective antimicrobial activity. Escherichia coli LPS were effectively removed from well plates by the experimental gel. Conclusions: These results demonstrated that this experimental gel is a useful tool for root conditioning of infected root surfaces and can also be applied for detoxification of ailing implant surface threads.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.3B
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• pp.153-160
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• 2007

Placing antennas of low power base stations below surrounding buildings, as in urban microcells, makes propagation characteristics strongly dependent on the building environment. As a result, propagation in these urban microcells is non-isotropic, so that the assumption of circular cells used in planning of conventional cellular sys toms is no longer valid. Assuming circular cells leads to a more conservative system design, implying more base stations. This work investigates the effect of cell shape, due to non-isotropic propagation, on the out-of-cell interference and Erlang capacity of CDMA system. Propagation is described by measurement derived models for low antennas in a rectangular urban street grid. The analysis is done for soft handoff protocols.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.10a
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• pp.519-522
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• 1997

In this study, the collision free path planning method of the articulated manipulator using sequential search is proposed. This method is to find the joint path of the manipulator with many degrees of freedom from the distal joint to the proximal one. To do this, the initial work space of the gantry manipulator, which is a remote maintenance equipment of the radioactive environment, is defined from the condition that the distal joint configuration is determined by the posture of maintenance. Then, 2-dimensional configuration space with the obstacle area is represented and the collision free path of manipulator is searched in the configuration space. And, this method is verified using the graphic simulation in virtual workcell for the spent fuel disassembling processes. The result of this study can be effectively used in implementing the maintenance processes for the hot cell equipment and enhance the reliability of the spent fuel management.

• Proceedings of the KIEE Conference
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• 1999.11c
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• pp.606-608
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• 1999

본 논문에서는 이동 로봇의 유연하고 짧은 경로 계획을 위하여 기존의 그리드 셀(grid cell)로 구성되는 지도 대신에 삼각형을 이용하여 지도를 구성하는 새로운 방법을 제안한다. 기존의 그리드 셀로 구성되는 지도의 경우 이동 로봇가 현재 셀에서 다음 셀로 진행시 진행 방향이 8 방향이나, 본 논문에서 제안한 방법에 의해 구성된 새로운 지도는 셀을 삼각형으로 표현함으로써, 이동 로봇의 진행 방향이 12 방향이 된다. 이와 같이 이동 로봇의 진행 방향이 증가한다는 것은 이동 로봇이 더욱 유연하게 장애물을 회피하며 더욱 짧은 경로로 주행할 수 있는 가능성을 의미한다. 한편, 본 논문에서 제안한 삼각형 지도 작성 방법의 효율성을 기존의 그리드 셀을 이용한 경로 계획 알고리즘인 거리 변환(path transform) 경로 계획을 통하여 증명한다. 또한 새로운 삼각형 지도 구성이 메모리의 효율성 위해 이동 로봇의 주행 환경을 장애물이 비어있는 공간을 가능한 하나의 셀로 병합하는 4진트리 방법(quadtree method)에도 적용 가능함을 보인다.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.8A
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• pp.829-837
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• 2008

A cellular system with fixed relays is considered to be a technology that can support high data transmission service to wide areas. However, either inter-cell interference or inter-sector interference that can be produced by adding relays to the cellular system with fixed relays does not guarantee high link performance to deteriorate function, so that resource allocation for avoidance of interference is very much important. In the paper, the cellular system performance with repeater relay has been compared with the cellular system performance with relay, and cell coverage expansion at the use of relay repeater has been compared. To compare, this paper has suggested resource allocation method to avoid inter-cell interference and inter-sector interference at installation of fixed relay on the cellular system. The proposed method can allocate different frequency resources on adjacent base stations and relays to reduce interference and to expand high data transmission area, and all of frequency bands are used at each sector to elevate efficiency of the frequency when base stations and relays operate simultaneously.

• Journal of Periodontal and Implant Science
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• v.47 no.2
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• pp.116-131
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• 2017

Purpose: The entry of bacteria or harmful substances through the epithelial seal of human gingival keratinocytes (HGKs) in the junctional epithelium (JE) is blocked by specialized intercellular junctions such as E-cadherin junctions (ECJs). However, the influence of roughened substrates, which may occur due to apical migration of the JE, root planing, or peri-implantitis, on the development of the ECJs of HGKs remains largely unknown. Methods: HGKs were cultured on substrates with varying levels of roughness, which were prepared by rubbing hydrophobic polystyrene dishes with silicon carbide papers. The activity of c-Jun N-terminal kinase (JNK) was inhibited with SP600125 or by transfection with JNK short hairpin RNA. The development of intercellular junctions was analyzed using scanning electron microscopy or confocal laser scanning microscopy after immunohistochemical staining of the cells for E-cadherin. The expression level of phospho-JNK was assessed by immunoblotting. Results: HGKs developed tight intercellular junctions devoid of wide intercellular gaps on smooth substrates and on rough substrates with low-nanometer dimensions (average roughness $[Ra]=121.3{\pm}13.4nm$), although the ECJs of HGKs on rough substrates with low-nanometer dimensions developed later than those of HGKs on smooth substrates. In contrast, HGKs developed short intercellular junctions with wide intercellular gaps on rough substrates with mid- or high-nanometer dimensions ($Ra=505.3{\pm}115.3nm$, $867.0{\pm}168.6nm$). Notably, the stability of the ECJs was low on the rough substrates, as demonstrated by the rapid destruction of the cell junction following calcium depletion. Inhibition of JNK activity promoted ECJ development in HGKs. JNK was closely associated with cortical actin in the regulation of ECJs in HGKs. Conclusions: These results indicate that on rough substrates with nanometer dimensions, the ECJs of HGKs develop slowly or defectively, and that this effect can be reversed by inhibiting JNK.

• Journal of Periodontal and Implant Science
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• v.29 no.4
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• pp.861-872
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• 1999

Since pathologic changes of exposed root surface inhibit cell attachment and new attachment of connective tissue have been made, many efforts were apply to change the exposed root surface condition. Scaling and root planing can not remove the endotoxin completely and forms the smear layer which prohibits the new attachment of connective tissue. Therefore, many kinds of chemicals were used for controlling the pathologic change of the root surface. The purposes of this study was to compare and observe the changes of the exposed root surface treated by scaling and root planning, Tetracycline HCl and Argon Laser. After the scaling and root planning of ten extracted premolars, the differences & the root surface among groups were observed under SEM. Control group showed smear layer and irregular amorphous surface. The dentinal tubule was not exposed. The debris and scale like texture were also observed. Tetracycline HCl treated group showed relatively smooth surface and the collagen fiber was observed in the dentinal tubule. Argon Laser treated group showed the most effective results under the conditions of 0.8 to 1.0w irradiation for 0.5 to 1.0 sec with pulse wave. The results of this study showed that the root surface change was associated with the intensity and the duration of Argon Laser irradiation. Further investigation for the surface change with the Argon Laser irradiation is recommended for understanding of clinical effect.