• Journal of Plant Biology
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• 제29권3호
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• pp.167-173
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• 1986

The effects of varying concentrations and durations of butachlor [N-(bytoxymethyl)-2-chlor-2', 6';-diethylacetanilide] treatment on oat (Avena sativa L.) root cell division were studied. Oats were treated from 0 to 48h with concentration ranging from 1$\times$10-6M to 1$\times$10-3M of butachlor. The highest concentration (1$\times$10-3M) of butachlor caused significant inhibition of cell division after 6h treatment. After 18h treatment, 49% and 66% inhibition of cell division occurred at 1$\times$10-5M and 1$\times$10-4M, respectively, while 16% inhibition of cell division occurred at 1$\times$10-6M concentration at same exposure period. Oat treated with 1$\times$10-5M and 1$\times$10-6M showed 69% and 38% inhibition of cell division after 36h. Increasing herbicide concentration at a specific time increased inhibition of cell division, and increasing the duration of treatment at a specific concentration also increased inhibition of cell division. In most instances the greatest inhibition of cell division occurred between 0 to 18h during 48h treatment. A range of concentration of 1$\times$10-5M to 1$\times$10-3M reduced cell enlargement significantly during 24h incubation period. The 1$\times$10-5M and 1$\times$10-3M caused 34% and 75% inhibition of cell enlargement. It was concluded that butachlor caused the growth inhibition of oats by inhibiting both cell division and cell enlargement.

• Journal of Plant Biotechnology
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• 제5권1호
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• pp.69-77
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• 2003

Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been extensively studied. The effect of brassinolide on the kinetics and final division frequencies of regenerating petal protoplasts of Petunia hybrida Vilm v. Comanche was examined. Under optimal auxin and cytokinin conditions, 10-100 nM brassinolide not only reduced the time of first cell division by 4.5 days but also altered the final division frequencies after 10 days of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 9%. Under sub-optimal auxin conditions, 10-100 nM brassinolide accelerated the first cell division, but no significant increase in the 8-10 days final division frequencies. Isolated protoplasts may provide a useful model system for the investigation of the molecular mechanisms of brassinosteroid action on cell division and proliferation in higher plants.

• Journal of Plant Biotechnology
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• 제5권1호
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• pp.63-67
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• 2003

Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been extensively studied. The effect of brassinolide on the kinetics and final division frequencies of regenerating petal protoplasts of Petunia hybrida Vilm v. Comanche was examined. Under optimal auxin and cytokinin conditions, 10-100 nM brassinolide not only reduced the time of first cell division by 4.5 days but also altered the final division frequencies after 10 days of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 9%. Under sub-optimal auxin conditions, 10-100 nM brassinolide accelerated the first cell division, but no significant increase in the 8-10 days final division frequencies. Isolated protoplasts may provide a useful model system for the investigation of the molecular mechanisms of brassinosteroid action on cell division and proliferation in higher plants.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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• pp.855-858
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• 2008

Transient current (TC) on bulk-state liquid crystal mixtures was measured. We found that TC is very sensitive to impurities and the features of TC curves depend on the type of contamination, from which the quality of materials can be definitely evaluated and the type of impurities can also be revealed.

• Molecules and Cells
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• 제19권1호
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• pp.46-53
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• 2005

Human embryonic stem (hES) cells, unlike most cells derived from adult or fetal human tissues, represent a potentially unlimited source of various cell types for basic clinical research. To meet the increased demand for characterized hES cell lines, we established and characterized nine new lines obtained from frozen-thawed pronucleus-stage embryos. In addition, we improved the derivation efficiency from inner cell masses (to 47.4%) and optimized culture conditions for undifferentiated hES cells. After these cell lines had been maintained for over a year in vitro, they were characterized comprehensively for expression of markers of undifferentiated hES cells, karyotype, and in vitro/in vivo differentiation capacity. All of the cell lines were pluripotent, and one cell line was trisomic for chromosome 3. Improved culture techniques for hES cells should make them a good source for diverse applications in regenerative medicine, but further investigation is needed of their basic biology.

• Molecules and Cells
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• 제27권1호
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• pp.127-127
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• 2009

• Molecules and Cells
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• 제21권1호
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• pp.29-33
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• 2006

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack $p16^{INK4a}$ regulatory function, compared to primary BME cells that were growth arrested by enforced expression of $p16^{INK4a}$. In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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• pp.1200-1203
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• 2009

A solution processed polymer tandem cell has been fabricated by using the organic layer coated crystalline $TiO_2$ nanoparticle inter layer. The highly dispersive OL-$TiO_2$ has several advantages in terms of excellent film forming property, crystallinity, optical transparency, and well defined chemical composition. The surface morphology of the $TiO_2$ thin film was found to play a crucial role in the performance of the polymer tandem cell. The stability of the tandem cell, utilizing dense $TiO_2$ nanoparticles inter layer, was superior to the stability of the single junction cell.

• Toxicological Research
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• 제21권1호
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• pp.77-85
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• 2005

Cadmium is an environmental pollutant exposed from contaminated foods or cigarette smoking and known to cause oxidative damage in organs. We investigated the cadmium-induced apoptosis and cell arrest in human breast cancer cells, MCF-7 cells and MDA-MB-231 cells. Obvious apoptotic cell death was shown in CdCl₂ 100 μM treatment for 12 hr, which were determined by DAPI staining and flow cytometric analysis. In cell cycle analysis, MCF-7 cells and MDA-MB-231 cells were arrested in S phase and G2/M phase respectively. These could be explained by the induction of cell cycle inhibitory protein, p21/sup Waf1/Cip1/ and p27/sup Kip1/, expression and reduction of cyclin/Cdk complexes in both cell lines. The decreased expression of cyclin A and Cdk2 in MCF-7 cells and cyclin B1 and Cdc2 in MDA-MB-231 cells were consistent with the flow cytometric observation. p-ERK expression was increased dose-dependent manner in both cell lines. It suggests that ERK MAPK pathway are involved in cadmium-induced cell cycle arrest and apoptosis. Moreover, cotreatment of zinc (100 μM, 12 hr) recovered the cadmium-induced cell arrest in both cells, which shows cadmium-induced oxidative stress mediates apoptosis and cell cycle arrest in human breast cancer cells.

• Fisheries and Aquatic Sciences
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• 제24권11호
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• pp.370-374
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• 2021

In this paper, we have reported the expression of immune-related gene in a new embryonic cell line (FGBC8) which was established from olive flounder (Paralichthys olivaceus) embryos. To explore the cell biotechnological applicability, the FGBC8 cells were incubated with the several mitogens such as lipopolysaccharide (LPS), polyinosinic-polycytidylic acid (poly I:C), flagellin, and interferon (IFN)-γ. After incubation, the expression of immune-related gene was observed in FGBC8 cells through the quantitative real-time PCR. Our results indicate that FGBC8 cells will serve as a valuable research tool for investigating host-pathogen interactions as well as cell biotechnological applications.