• YAKHAK HOEJI
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• v.52 no.5
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• pp.412-417
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• 2008

BAY11-7082 was initially found to be an anti-inflammatory drug with NF-${\kappa}B$ inhibitory property. In this study, we evaluated modulatory function of BAY11-7082 on U937 cell-cell adhesion induced by CD29 (${\beta}1$-integrins). BAY11-7082 strongly blocked functional activation of CD29 (${\beta}1$-integrins), as assessed by cell-cell adhesion assay. However, this compound did not block a simple activation of CD29, as assessed by cell-fibronectin adhesion assay. In particular, to understand molecular mechanism of BAY11-7082-mediated inhibition, the regulatory roles of CD29-induced actin cytoskeleton rearrangement under cell-cell adhesion and surface level of CD29 were examined using confocal and flow cytometic analysis. Interestingly, this compound strongly suppressed the molecular association of actin cytoskeleton with CD29 at cell-cell adhesion site. Moreover, BAY11-7082 also diminished surface levels of CD29 as well as its-associated adhesion molecule CD147, but not other adhesion molecules such as CD18 and CD43. Therefore, our data suggest that BAY11-7082 may be involved in regulating immune responses managed by CD29-mediated cell-cell adhesion.

• Journal of Korean Society of Water and Wastewater
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• v.32 no.2
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• pp.153-158
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• 2018

In this study, effects of nutrient and inorganic carbon on single cell emergence during the cultivation of microalgae were observed using colonial green algae, Pediastrum duplex. The concentration of inorganic carbon had significant effect on single cell emergence and its growth, but nitrogen and phosphorus concentration showed minor effects. According to P. duplex cultivation experiment, single cell started to be emerged around 500~750 mg-C/L of inorganic carbon concentration and it was bloomed dramatically at the higher values. And growth of P. duplex was started to be surpressed at the single cell formation concentration. From the results, it could be said that when we operate the microalgae systems for cultivation/harvesting or wastewater treatment, in order to avoid single cell formation, inorganic carbon should be maintained to the proper level.

• Journal of the Korean Chemical Society
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• v.7 no.1
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• pp.58-64
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• 1963

Authors propose a new technique using polyethylene film instead of sodium chloride window as a cell material. Nujol mulls, liquids and aqueous solutions are sandwitched between two pieces of polyethylene film which are held between cardboards. Ordinary lead or stainless steel spacers could be used if exact cell thickness is desired. A more elaborate cell can be assembled by injecting samples between two pieces of polyethylene film which are placed between sodium chloride windows of ordinary demountable liquid cell. The absorption bands due to polyethylene and Nujol are compensated by placing the polyethylene film of suitable thickness in the reference beam. The absorption bands due to solvents such as water can also be compensated by the polyethylene film cell sandwitched solvent of suitable thickness in the reference beam. This method would be a simple new technique. Especially this technique may offer a new helpful way for the investigation of the state of substances in aqueous system. Using this technique, authors have observed the appearance of an absorption bands at 3.2 micron, in the spectrum of phenol in aqueous solution, that is absent in the spectrum of phenol in benzene solution. The same absorption band also has been observed in the spectra of aqueous formaldehyde solution and aqueous polyvinyl alcohol solution, where the absorption bands due to polyethylene and water are compensated. Although it may be regarded that this absorption band is related to the intermolecular interaction between water and the solute having OH group, that is hydrogen bonding. The exact assignment of this absorption band is out of this work.

• Biomedical Science Letters
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• v.10 no.2
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• pp.85-92
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• 2004

Immortalization of primary corneal cells has influence on pharmacy, medical and biological fields. Especially, investigation of immortalization mechanism using viral oncoproteins is useful for medical treatments, and these cell lines will be useful materials for toxic test of medical supplies and cell biological experiments. Rabbit corneal fibroblasts in culture undergo a finite number of divisions before they reach a terminally non-proliferating state known as replicative senescence. Therefore, we attempted to induce immortalization of rabbit corneal fibroblasts with SV 40 large T antigen. As a result of experiment, expression of SV 40 large T antigen was confirmed, and expression of proteins related to cell cycle repressor was decreased in the transfection group compared with non-transfection group. According to the results of cell cycle phase distribution test, SV 40 large T antigen-transfected cells had obtained higher proliferation rate than primary cells. It was confirmed that during induction of immortalization, SV 40 large T antigen was not able to increase telomerase activity. In conclusion, we made a rabbit corneal fibroblast cell line with SV40 large T antigen. This cell line will be useful for further studies of mammalian fibroblast biology, particularly with regard to angiogenesis and malignant transformation. In addition, this cell line offers opportunity for testing potential therapeutics and can be used for toxicity tests of materials or cosmetics. In the future, our cell line can potentially be utilized in a wide range of biology related fields.

• Journal of Sasang Constitutional Medicine
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• v.19 no.2
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• pp.170-178
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• 2007

1. Objective This study was aimed to screen the potential antitumor activity of one kinds of Korean medicinal herb extracts against cancer cell lines and to evaluate the growth inhibition effect of L-1210 and S-180 cancer cell lines. 2. Methods It confirmed Anemarrhena asphodeloides extracts to screen the potential antitumor activity. Then, it was extracted with 4 kinds of solvents ; hexane, ethyl acetate, butanol and $H_2O$, and the Growth inhibition effect of these extracts were determined against cancer cell and normal cell. The results were as follows : The IC50(50% inhibitory concentration) values of Anemarrhena asphodeloides extracts were shown to be $253{\mu}g/ml$ against L-1210 cell lines. The IC50 values of ethyl acetate extracts were shown to be $915{\mu}g/ml$ against L-1210 cell lines. The IC50 values of butanol extracts were shown to be $52.3{\mu}g/ml$, $485{\mu}g/ml$ against L-1210, S-180 cell lines, respectively. The butanol extracts were more selectively effective than other extracts to cancer cell lines. 3. Conclusion From these data, it could be concluded that the Anemarrhena asphodeloides extracts to the Growth inhibition effect of L-1210 and S-180 cancer cell lines.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.526-526
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• 2013

Plasma technology isbeing developed for a range of medical applications including wound healing. However, the effect of plasma on many cells and tissues is unclear. Cell migration and cell proliferation are very important biological processes which are affected by plasma exposure and might be a potential target for plasma therapy during wound healing treatment. In this study, we confirmed the plasma exposure time and incubation time after plasma treatment in skin fibroblast (L-929 cells) to evaluate the optimal conditions forplasma exposure to the cell in-vitro. In addition, we used a scratch method to generate artificial wound for evaluating the cell migration by plasma treatment. Where, the cells were treated with plasma and migration rate was observed by live-cell imaging device. To find the cell proliferation, cell viability assay was executed. The results of this study indicate the increased cell proliferation and migration on mild plasma treatment. The mechanisms for cell migration and cell proliferation after plasma treatment for future studies will be discussed.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.65-72
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• 2020

Fish ovarian germline stem cells (OGSCs) that have the abilities to self-renew and differentiate into functional gametes can be used in various researches and applications. A main issue to be solved for effective utilization of fish OGSCs is the development of their stable in vitro culture condition, but only few researches about fish OGSC culture have been reported so far. In this study, in order to find the clues to develop the culture condition for OGSCs from Japanese medaka (Oryzias latipes), we tried to establish somatic cell lines as a candidate for the feeder cells and evaluated its supporting effects on the culture of ovarian cell populations from O. latipes. As the results, the somatic cell lines could be established only from the embryonic tissues among three tissues derived from embryos, fins and ovaries. Three embryonic cell lines were tested as a feeder cell for the culture of ovarian cell population and all three cell lines induced cell aggregation formation of the cultured ovarian cells whereas the feeder-free condition did not. Furthermore, a significant cellular proliferation was observed in the ovarian cells cultured on two of three cell lines. As a trial to increase the capacity of the cell lines as a feeder cell that supports the proliferation of the cultured ovarian cells, we subsequently established a stable line that expresses the foreign O. latipes fibroblast growth factor 2 (FGF2) from an embryonic cell line and evaluated its effectiveness as a feeder cell. The ovarian cells cultured on FGF2 expressing feeder cells still formed cell aggregates but did not show a significant increase in cellular proliferation compared to those cultured on non-transformed feeder cells. The results from this study will provide the fundamental information for in vitro culture of medaka OGSCs.

• BMB Reports
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• v.36 no.1
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• pp.60-65
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• 2003

Cancer is frequently considered to be a disease of the cell cycle. As such, it is not surprising that the deregulation of the cell cycle is one of the most frequent alterations during tumor development. Cell cycle progression is a highly-ordered and tightly-regulated process that involves multiple checkpoints that assess extracellular growth signals, cell size, and DNA integrity. Cyclin-dependent kinases (CDKs) and their cyclin partners are positive regulators of accelerators that induce cell cycle progression; whereas, cyclin-dependent kinase inhibitors (CKIs) that act as brakes to stop cell cycle progression in response to regulatory signals are important negative regulators. Cancer originates from the abnormal expression of activation of positive regulators and functional suppression of negative regulators. Therefore, understanding the molecular mechanisms of the deregulation of cell cycle progression in cancer can provide important insights into how normal cells become tumorigenic, as well as how cancer treatment strategies can be designed.

• 한국정보디스플레이학회:학술대회논문집
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• 2008.10a
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• pp.855-858
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• 2008

Transient current (TC) on bulk-state liquid crystal mixtures was measured. We found that TC is very sensitive to impurities and the features of TC curves depend on the type of contamination, from which the quality of materials can be definitely evaluated and the type of impurities can also be revealed.

• Journal of information and communication convergence engineering
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• v.8 no.4
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• pp.393-398
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• 2010

For multi-cell environments, coordinated random beamforming technique in multiuser MIMO(multiple-input multiple-output) broadcast channel is considered. In order to mitigate severe interference at receivers, the multi-cell environments might require complex transmitter and receiver design because the scheduler decision based on full channel state information (CSI) in one cell must be intertwined with decision made by other cells' CSI. With limited CSI, however, this paper considers a scheme of randomizing transmitters' beamforming but being coordinated with other cell transmitters. The transmitters in each cell share random beamforming patterns and schedule data transmission within coherent scheduling period. The corandomized beams allow the users to be selected with the highest SINRs even in multi-cell environments. We analyze the performance of the proposed scheme. And numerical results show that the scheme achieves better performance than the conventional random beamforming when applying to multi-cell environments.