• Title/Summary/Keyword: Cell Area

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Enhanced Production of Digoxin by Digitoxin Biotransformation Using In Situ Adsorption in Digitalis lanata Cell Cultures

  • Hong, Hee-Jeon;Lee, Jong-Eun;Ahn, Ji-Eun;Kim, Dong-Il
    • Journal of Microbiology and Biotechnology
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    • v.8 no.5
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    • pp.478-483
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    • 1998
  • For the enhanced production of a cardiac glycoside, digoxin, using in situ adsorption by biotransformation from digitoxin in plant cell suspension cultures, selection of proper resins was attempted and the culture conditions were optimized. Among various kinds of resins tested, Amberlite XAD-8 was found to be the best for digoxin production in considering adsorption characteristics as well as the effect on cell growth. Adequate time for resin addition was determined to be 36 h from the beginning of biotransformation and the presence of resins should be as short as possible to increase the productivity. In addition, to prevent the cells from direct contact with resin particles, immobilized systems were designed and examined. Immobilization further improved the advantages of in situ adsorption. It was confirmed that the increase of the contact area for mass transfer was an important factor in utilizing an immobilized system to enhance digoxin production.

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A Computational Study of the Fuel-Cell Ejector System (연료전지 이젝터 시스템에 관한 수치해석적 연구)

  • Lee, Jun-Hee;Lee, Hae-Dong;NamKoung, Hyuck-Joon;Kim, Heuy-Dong
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.3191-3196
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    • 2007
  • The present study addresses a method to operate a fuel-cell system effectively using a recirculation ejector which recycles wasted hydrogen gas. Configuration of a recirculation ejector is changed to investigate the flow behavior through it under varying operating conditions, and how such conditions affect the fuel-cell hydrogen cycle. The numerical simulations are based on a fully implicit finite volume scheme of the axisymmetric, compressible, Reynolds-Averaged, Navier-Stokes equations for hydrogen gas, and are compared with available experimental data for validation. The results show that a hydrogen recirculation ratio is effectively controlled by a configurational alteration within the operational region in which the recirculation passage doesn't plugged by a sonic line.

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A 10-bit Current-steering DAC in 0.35-μm CMOS Process

  • Cui, Zhi-Yuan;Piao, Hua-Lan;Kim, Nam-Soo
    • Transactions on Electrical and Electronic Materials
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    • v.10 no.2
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    • pp.44-48
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    • 2009
  • A simulation study of a 10-bit two-stage DAC was done by using a conventional current switch cell. The DAC adopts the segmented architecture in order to reduce the circuit complexity and the die area. The 10-bit CMOS DAC was designed in 2 blocks, a unary cell matrix for 6 MSBs and a binary weighted array for 4 LSBs, for fabrication in a 0.35-${\mu}m$ CMOS process. To cancel the accumulation of errors in each current cell, a symmetrical switching sequence is applied in the unary cell matrix for 6 MSBs. To ensure high-speed operation, a decoding circuit with one stage latch and a cascode current source were developed. Simulations show that the maximum power consumption of the 10-bit DAC is 74 mW with a sampling frequency of 100 MHz.

Numerical Simulation of the Elastic Moduli of Cement Paste As a Three Dimensional Unit Cell

  • Park, Ki-Bong
    • Architectural research
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    • v.12 no.2
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    • pp.93-98
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    • 2010
  • This paper describes a numerical method for estimating the elastic moduli of cement paste. The cement paste is modeled as a unit cell which consists of three components: the unhydrated cement grain, the gel, and the capillary pore. In the unit cell, the volume fractions of the constituents are quantified using a single kinetic function calculating the degree of hydration. The elastic moduli of cement paste are calculated from the total displacements of constituents when a uniform pressure is applied to the gel contact area. The cement paste is assumed to be a homogenous isotropic matrix. Numerical simulations were conducted through the finite element analysis of the three-dimensional periodic unit cell. The model predictions are compared with experimental results. The predicted trends are in good agreement with experimental observations. This approach and some of the results might also be relevant for other technical applications.

A Study on the Improvement of Efficiency by Scribing Transparent Conducting Oxide of Dye-sensitized Solar Cell (염료감응형 태양전지의 투명 전극 식각을 통한 효율 향상 연구)

  • Seo, Hyun-Woong;Son, Min-Kyu;Lee, Kyoung-Jun;Kim, Jeong-Hoon;Kim, Hee-Je
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.05a
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    • pp.416-418
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    • 2008
  • Dye-sensitized solar cell using transparent conducting oxide as electrode has large resistance such as surface resistance, charge transportation impedance in counter electrode and electrolyte, impedance between each interface. Among that resistances, surface resistance of transparent conducting oxide is relatively large. So the change of transparency has a large effect on internal resistance of dye-sensitized solar cell. Consequently, that change cause to increase or decrease the conversion efficiency. We tried to reduce the surface resistance by laser-scribing. The active area is seperated from total transparent conducting oxide by Nd:YAG laser-scribing. As a result, we achieved the improvement of efficiency about 7% and 11% in case of $0.25cm^2$ and $1.00cm^2$ dye-sensitized solar cells.

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Immunocytolocalization of Cell Wall Peroxidase and Other Wall Antigens from Maize Seedlings

  • Kim, Sung-Ha
    • Journal of Plant Biology
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    • v.39 no.2
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    • pp.99-105
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    • 1996
  • Immunocytochemistry utilizes the specificity of the antigen-antibody reaction to localize specific antigens in cells or cellular organelles. Here we report the use of monoclonal antibodies, in conjunction with gold-labeled second antibodies to study the ultrastructural localization and tissue distribution of the Mr 98, 000 anionic peroxidase and other wall antigens. The antibody specific for this wall peroxidase, mWP3, labeled mainly the cell wall area. At the tissue level, the Mr 98, 000 peroxidase is located predominantly in the leaf mesophyll, internal coleoptile and sieve elements, but not in the root, as assayed with these procedures. The coleoptile walls were less heavily stained than the walls of leaf mesophyll cells. At the subcellular level, it is localized mainly in intercellular regions of the cell walls. A similar staining pattern was revealed by mWP19, one of anti-$\beta$ glucosidase antibody, though it looked less heavily stained than one with mWP3. In order to serve as a control wall staining using IgM monoclonal antibodies, mWP18 was used. Most of the label is localized over wall regions of cells of the young leaf mesophyll and coleoptile.

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Applications of AGNPS model with rural watersheds having complex land use characteristics (복합 토지이용 특성의 농촌유역에 대한 농업비점원오염모형의 적용)

  • 조재필;박승우;강문성
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 1998.10a
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    • pp.353-358
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    • 1998
  • GRASS-AGNPS model integrated with GIS was applied to rural watersheds having complex land use characteristics and evaluated for its applicability through calibration using observed data. The analyses of raster encoding accuracy and model behavior to runoff, sediment yields and nutrient loads for different cell-size showed that 150 m cell size indicated reasonable applicability of the model. Simulated runoff was in a good agreement with the observed data and simulated peak runoff rate was larger than the observed data. The sediment yield simulated by modified AGNPS model using irregular cell for forest area were less than that of the regular cell method. In predicting sediment yields, the result showed a different trend at each representative rural watershed. Nutrient loads simulated by the model were significantly different from the observed data.

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Application of Single Cell Gel Electrophoresis for Detection of DNA Single Strand Breaks in DNA of Fish Blood Cell (어류혈구세포에 있어서 Single Cell Gel Electrophoresis를 응용한 DNA Single Strand Breack의 측정)

  • KIM Gi Beum;LEE Richard F.;MARUYA Keith A.
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.4
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    • pp.346-351
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    • 2003
  • Single-cell gel electrophoresis (comet assay) was used to detect DNA single strand break in blood cells from several marine fish species. Three fish species were collected from Georgia coastal area. Mummichog, Fundulus heteroclitus showed higher DNA damage than sea bass, Lateolabrax japonicus and trout, Oncorhynchus masou masou under the same experimental conditions. Mummichogs had more alkaline-labile sites on their DNA than other fish species. The comet assay with mummichog blood cells at pH 12.5 showed a dose-response curve with the increasing concentrations of hydrogen peroxide. While the isolated leucocytes showed no increase of DNA damage after in vitro exposure to 2-methyl-1,4-naphthoquinone (MNQ), erythrocytes showed dose-dependent DNA damage. These results indicate that the comet assay can be applied successfully as a bioassay using erythrocyte for environmental monitoring.

Current characteristics of Cu/NaCl electrolyte/Zn electrochemical cell (구리/NaCl 전해질/아연 전기화학전지의 전류특성)

  • Kim, Yong-Hyuk
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.59 no.9
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    • pp.1626-1631
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    • 2010
  • The characteristics of electric current for the voltaic cell are important for electric power applications. In this paper, an electrical equivalent model consist of three resisters and a capacitance for the Cu/NaCl solution/Zn electrochemical cell is proposed. The capacitance which exists in the Zn electrode/electrolytic interface increased according to Zn electrode area, but cannot affect almost in electric current. Complex impedance plot was used to analysis the interface effect for Zn/electrolyte. This result shows that the interface is similar with the electric transmission line. The short current measurements were conducted to investigate the effects of hydrogen peroxide, the watery sulfuric acid and NaCl aqueous solution. As the hydrogen peroxide increased, the electric current increased because the hydrogen gas being converted with the water. Also electric current increased significantly with increase of the hydrogen ion with the watery sulfuric acid and increased with increase of $Na^+$ ion and $Cl^-$ion in the NaCl electrolyte.