• Fisheries and Aquatic Sciences
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• 제27권6호
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• pp.366-378
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• 2024

Application of commercial hormone failed to promote breeding in certain Pangasius species due to the differences of gonadotropin-releasing hormone specific peptide with species-specific bioactivities. Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide in the reproductive system that plays a crucial role in the regulation of reproductive processes. This study was performed to determine and analyse the GnRH genes from commercially important Pangasius sp., Pangasianodon hypophthalmus and Pangasius nasutus. The GnRH1 and GnRH2 genes were amplified and cloned into TOPO vector, followed by phylogenetic analysis of a complete open reading frame (ORF) of GnRH genes. The GnRH1 and GnRH2 genes of P. hypophthalmus and P. nasutus were detected at 300 bp and 360 bp, encoded for 81 and 87 amino acids, respectively. Amino acid sequence identities revealed high homology of P. hypophthalmus and P. nasutus GnRH1 and GnRH2 genes in comparison with other fish and vertebrates. Phylogenetic tree showed that fish from various families were aggregated into a group of the same order due to their highest identity similarities. It revealed that the vertebrate formed clusters and are grouped according to their GnRH decapeptide and GnRH-associated peptide (GAP) region, indicating a close relationship among GnRH decapeptide and GAP in different vertebrate species.

• 한국발생생물학회지:발생과생식
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• 제6권2호
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• pp.71-76
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• 2002

최첨단 연구로 성장하고 있는 해양생물공학 연구 중 염색체공학 연구의 배수체 조작, 성분화, 자성 발생, 형질전환 어류와 유전자 조작에 관한 최근의 연구 동향을 분석하고 향후 발전 방향에 관하여 살펴보았다. 배수체 연구 중 3배체 어류 생산 연구는 20여종의 어류들 중 무지개 송어와 메기가 물리적 충격을 이용한 실험법으로 산업적으로 이용 가능한 수준에 있다. 성분화를 포함한 약 15종의 고급 어종이 방사선 투사법에 의해 자성 발생 2배체를 생산 유도하였다. 형질전환 어류는 1985년 이래 약 40여종의 어류가 외래 유전자를 미세 조작이나 전기충격법에 의해 형질전환되고 있다. 최근에 역바이러스성 벡터들에 의한 외래 유전자를 이용한 형질전환 어류가 생산되었으며 이러한 형질전환 어류는 기초과학 육성은 물론 해양생물공학 연구의 발전이 기대되고 있다.

• 한국조리학회지
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• 제14권2호
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• pp.61-72
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• 2008

The purpose of this study was to analyze the difference in preference and property of native local foods among students living in Busan. It concentrated on the students who were majored in food service in five universities in Busan. From September 1st to 30th, 2007, 300 copies of questionnaire were distributed and 260 returned were included in the statistics. The statistics was conducted by frequency analysis, T-test(average deviation verification), technical analysis and one way ANOVA for an accurate result of the study. As a result, it was found that Gayamilmyeon(M=4.42) showed the most preference for 25 native local foods of Busan(sansungmakgeoli, dongraepajeon, steamed carp, catfish soup, corbicula soup, roasted eel, gijang raw hairtail, dongrae mugwort cake, sansung roasted goat, gijangkkomjangeo, steamed frogfish, duck dish, guponoodles, gayamilmyeon, sliced raw fish, molsim hot-pepper fish stew, hairtail stew, anchovy stew, miyeoksulchi, gompi salted dish, salted raw anchovy dish, roasted kkomjaneo, salted sliced persimmon dish, salted bean leave dish, kkasiri soup). On the other hand, it was found that Miyeoksulchi(M=2.66) showed the least preference among them. As a result of the analysis of the difference in demographic characteristics and property of native local foods, it was presented that there was no difference in the statistical analysis by sex but significant difference in taste, quantity, service, price, freshness, sanitation, packing condition and originality, etc by age, department, academic year, type of residence, family member, living period in Busan and monthly spending. It was considered to conduct a more in-depth study to promote native local foods and interest in the food of Busan in university students.

• 한국해양바이오학회지
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• 제3권1호
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• pp.1-6
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• 2008

Angola has a coastline of about 1,650 km long. Two diverging current namely, the Angola current with its warm water from the north and the cold Benguella Current in the south create a strong up-welling with a high productive ecosystem for marine resources. The area from Lobito to the mouth of the Cunene River, also known as the Southern fishing zone is by far the most productive of Angola's fishing zones. In 1977, the total potential of its marine fisheries sector was estimated at more than 700,000 tonnes per annum. In 2003, the Total Allowable Catch (TAC) established for demersal species was 57,600 tonnes and 160,000 tonnes for pelagic species. The most important resources are various marine demersal and pelagic fish including pilchard and the Cape and Cunene horse Mackerel (Tranchurus capensis and T. trecae). Sardinellas (Sardinella aurita and S. maderensis) are fished in parallel with horse mackerel. The rest of the catches are mainly demersal spp. and some deep water crustaceans. The demersal sppecies consist of Hake (Merluccius polli and M. capensis) and the large eye dentex spp. Tunas are caught at certain times of the year whilst some marine shrimp are also harvested from the Angolan waters. Angola also has several high value freshwater fish species, exploited by about 255 fishers. Tilapia sp. is among the most important and abundant fresh water fish found in Angola. Other species include the catfish (Clarias gariepinus) and fresh water prawns (Macrobrachuin rosenbergii). Some aquaculture ponds have been established in the country side, but due to lack of investment, proper training and the impact of civil war have seriously limited developments in the sub sector.

• 한국어병학회지
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• 제3권1호
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• pp.21-25
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• 1990

내수면(內水面) 가두리 양식장(養殖場)의 그물에 매년(每年) 부착(附着), 성장(成長)하여 물의 소통(疏通)을 억제(抑制)시킬 뿐 만 아니라, 사육어(飼育魚)를 체포(逮捕)하거나 어망교체시(漁網交替時) 망(綱)에 부착(附着)한 이끼벌레와 접촉(接觸)되므로서 어류폐사상태(魚類斃死狀態), 각종 약제(藥劑)에 의한 구제실험(驅除實驗)을 실시하였다. 1) 옥정호 가두리 어망(漁綱)에 부착(附着)된 이끼벌레는 분류상(分類上) 태형동물문(Bryozoa), 암후강(Phylactolaemata)에 속하는 총담이끼벌레, Lophopodella carteri HYATT이다. 2) 군체(群體)의 크기는 $0.8{\sim}8.5{\times}0.6{\sim}5.0cm$이었다. 3) 수온(水溫) $25.7^{\circ}C$인 6월(月) 하순(下旬)에 부착(附着)하기 시작하여 수온(水溫) $22^{\circ}C$때인 10월(月)에 최대(最大)크기로 성장(成長)되고, 수온(水溫) $8.5^{\circ}C$ 12월(月) 중순(中旬)에 군체(群體)는 수축(收縮)되면서 부착물(附着物)에서 소실(消失)되어진다. 4) 표층(表層)에서 5m층(層)까지 부착(附着)되나, 2~3m층(層)에 주로 부착(附着)한다. 5) 수온(水溫) $25^{\circ}C$때 이끼벌레와 1분간(分間) 접촉(接觸)시킨 전장(全長) $4^{\circ}C$ 17cm의 이스라엘 잉어 30마리와 3~7cm 금붕어 30마리는 1시간(時間) 40분(分) 경과(經過) 후(後) 이스라엘잉어는 90%, 금붕어는 100% 폐사(斃死)되었으나, 메기는 전혀 폐사(斃死)하지 않았다. 6) 어류(魚類)에 피해(被害)를 주는 이끼벌레는 촉수주위(觸手周圍)에 있는 자포(刺胞)에 의한 것으로 추정(推定)되며, 이 자포독(刺胞毒)은 잉어, 금붕어에 맹독성(猛毒性)을 나타낸다. 7) Formalin 300ppm, 식염(食鹽) 5%, malachite green 5 ppm, $KMnO_4$ 200ppm, Kl 1000ppm, DDVP 10 ppm에 이끼벌레를 10분간(分間) 침적(沈積)시켰으나 전부 생존(生存)하였다.

• 한국어류학회지
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• 제30권1호
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• pp.65-68
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• 2018

섬진자가사리 Liobagrus somjinensis 후각기관과 서식처 환경 및 생태적 습성의 연관성을 규명하기 위해 실체현미경과 디지털카메라를 이용하여 해부학적 특징을 묘사하였다. 후각기관은 주둥이 위에 좌우 한 쌍으로 존재하였으며, 전비공, 후비공, 비강으로 크게 구성되어 있었다. 전비공은 튜브형의 형태로 코수염과 윗입술 끝 사이에 위치하였다. 후비공은 표면과 평평한 유출공으로써 코수염의 기부 바로 아래에 위치해 있었다. 비강은 가로와 방사형으로 배열된 혀 모양의 22~24개의 판으로 구성된 로제트 구조를 보유하고 있었다. 결과적으로 섬진자가사리의 후각기관의 기능 해부학적 구조는 여울에서의 은신, 섭식, 야행성의 생활패턴에 연관된 높은 후각의존도를 반영하는 것으로 생각된다.

• 한국식품위생안전성학회지
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• 제35권1호
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• pp.45-50
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• 2020

본 연구는 원주시 내 대형 초밥 뷔페에서 제공되는 초밥, 회 등 26개 수산물 가공품을 대상으로 DNA 바코드를 분석하여 원재료의 종을 동정하였다. DNA 바코드 증폭을 위하여 미토콘드리아의 16S ribosomal RNA 및 cytochrome c oxidase subunit I 유전자 부위를 증폭하는 프라이머 세트를 이용하여 증폭된 PCR 산물의 염기서열을 분석하였다. 확보한 염기서열은 BLAST Search를 이용하여 미국국립보건원 GenBank에 등록되어있는 생물 종의 염기서열과 비교하여 염기서열 유사도와 매칭 점수를 고려하여 최종 종을 동정하였다. 모니터링 결과 분석 제품의 58%는 제품명과 사용된 원재료가 일치하였지만, 27%에서는 불일치가 관찰되었다. 초메기초밥에는 가이양(Pangasianodon hypophthalmus)이 꽃돔회에는 붉평치(Lampris guttatus)가 사용되었으며, 날치알군함 및 청어알무침에는 열빙어(Mallotus villosus) 알이 사용되었음을 확인하였다. 타코와사비군함 및 오징어간장소스에 사용된 원재료는 주꾸미(Amphioctopus fangsiao) 및 남방주꾸미(Amphioctopus membranaceus)로 각각 동정되었다.

• 한국수산과학회지
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• 제52권5호
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• pp.482-495
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• 2019

This study compared the productivity of fish and vegetables grown using the hybrid biofloc technology-aquaponics (HBFT-AP), a semi-recirculating aquaculture system (SRAS), and hydroponics (HP). For the study of fish productivity (HBFT-AP vs. SRAS), fish were provided feed containing 3.0% monobasic potassium phosphate (MKP) for 18 weeks. After the 18-week feeding trial, the average weight of the sampled population (n=100) was not significantly different (P>0.05), while hematocrit (PCV, %), hemoglobin (Hb, g/dL), and plasma K (mEq/L) were significantly different (P<0.05) between the two groups (HBFT-AP: 47.83%, 15.48 g/dL, and 1.39 mEq/L; SRAS 34.83%, 11.81 g/dL, and 2.48 mEq/L). Leaf vegetable productivity (HBFT-AP vs. HP) was compared in three experiments (EXP 1-3), and slower growth was observed in both groups in EXP 2, in which pH was maintained at 5.0 or less throughout the experiment. During the 18-week feeding trial, total ammonia nitrogen (TAN), $NO_3-N$, and $PO_4-P$ levels increased with time in the HBFT-AP system, while the concentration of $NO_2-N$ remained below 0.1 mg/L throughout the study.

• 한국식품위생안전성학회지
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• 제35권5호
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• pp.447-451
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• 2020

본 연구에서는 2018년 충남지역에서 생산, 출하되는 수산물에 대해 잔류동물용의약품 실태를 조사하였다. 수산물 115건을 대상으로 식품공전 수산물 중 동물용의약품 동시 다성분 시험법으로 분석이 가능한 29종의 잔류동물용의약품을 분석하였다. 115건의 수산물 중 9건에서 6종의 잔류동물용의약품이 검출되었으며, 검출률은 7.8%이었다. 검출된 잔류동물용의약품은 옥소린산, 엔로플록사신이 가장 많이 검출되었으며 시프로플록사신, 설파디아진, 플루메퀸, 옥시테트라사이클린이 각각 검출되었다. 미꾸라지 검체 1건에서 엔로플록사신이 잔류기준을 초과하였으나 전체적으로 안전한 수준으로 평가되었다. 그렇지만 잔류동물용의약품 검출률이 높아지는 추세이고 종류 또한 다양해지고 있어 지속적인 모니터링이 필요하다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권6호
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• pp.2761-2765
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• 2016

Opisthorchiasis is a health problem in rural communities of Thailand, particularly in the northeast and north regions. Therefore, this study aimed to investigate raw fish consuming behavior related to liver fluke infection among the population at risk for opisthorchiasis and cholangiocarcinoma (CCA). A cross-sectional descriptive study was conducted in Meuang Yang district, Nakhon Ratchasima province, northeast Thailand, between June and October 2015. Participants were screened for CCA, and samples who had a high score of CCA risk were purposively selected. A predesigned questionnaire was utilized to collect the data from all participants. $X^2-test$ was used for analysis of associations between demographic data and raw fish consumption. The results revealed that participants had past histories of stool examination (33.0%), liver fluke infection (21.0%), praziquantel use (24.0%), raw fish consumption (78.0%), relatives family consuming raw fish (73.0%), and relatives family with CCA (3.0%). Participants consumed several dished related to liver fluke infection, mainly raw fermented fish (13.0%), under smoked catfish (5.00%), raw pickled fish (4.00%), and raw spicy minced fish salad (3.00%). The most common types of cyprinoid fish were Barbodes gonionotus (39%), Hampala dispar (38%), Puntius brevis (37%), Cyclocheilichthys armatus (33%), Puntioplites proctozysron (32%), and Luciosoma bleekeri (30%), respectively. Participants had a low level of knowledge (mean=3.79, SD=0.74), moderate attitude (mean=7.31, SD=7.31) and practice (mean=38.64, SD=6.95) regarding liver fluke prevention and control. Demographic variables like age (>36 years old; $X^2-test=17.794$, p-value=0.001), education (primary school; $X^2-test=18.952$, p-value=0.001), marital status (married; $X^2-test=12.399$, p-value=0.002), and income (<5,000 baht; $X^2-test=27.757$, p-value=0.015) were significantly associated with raw fish consumption. This result indicates that the population had risk consumption for liver fluke infection particularly of various cyprinoid fishes that are $2^{nd}$ intermediate hosts. Therefore, health education is required to improve their behavior.