• Bulletin of the Korean Chemical Society
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• v.30 no.7
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• pp.1497-1504
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• 2009

GC/MS analysis of catecholamines (CAs) in biological sample may produce poor reproducible quantitaion when chemical derivatization is used as the technique to form a volatile derivative. Significant quantities of the side products can be formed from CAs with primary amine during the derivatization reaction under un-optimized conditions. We have tested various chemical derivatization techniques in an attempt to find an optimum derivatization method that will reduce side product formation, enable to separate several catecholamine derivatives in GC chromatogram, and obtain significant improvement of detection sensitivity in GC/MS analysis. Whereas several derivatization techniques such as trimethylsilylation (TMS), trifluoroacylation (TFA), and two step derivatization methods were active, selective derivatization to form O-TMS, N-heptafluorobutylacyl (HFBA) derivative using N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) and N-methyl-bis(heptafluorobutyramide) (MBHFBA) reagents was found to be the most effective method. Moreover, this derivative formed by selective derivatization could provide sufficient sensitivity and peak separation as well as produce higher mass ion as base peak to use selected ion in SIM mode. Calibration curves based on the use of an isotopically labeled internal standard show good linearity over the range assayed, 1 ~ 5000 ng/mL, with correlation coefficients of > 0.996. The detection limits of the method ranged from 0.2 to 5.0 ppb for the different CAs studied. The developed method will be applied to the analysis of various CAs in biological sample, combined with appropriate sample pretreatment.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.11a
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• pp.195-195
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• 2008

CAS계 유리에 $CaCO_3-Al_2O_3$ 혼합물 및 화합물을 10, 30 wt% 첨가하여 저온 소걸 및 마이크로파 유전 특성을 고찰하였다. CAS계 유리의 연화온도는 $841^{\circ}C$ 이며, CAS계 유리에 $CaCO_3$ 와 30 wt%의 $CaCO_3-Al_2O_3$ 혼합물을 melting되며, 10 wt%의 $CaCO_3$, $Al_2O_3$, $1CaCO_3-1Al_2O_3$ 혼합물 및 $CaAl_2O_4$ 화합물를 10 wt% 첨가하였을 때 $900^{\circ}C$ 이하에서 소걸이 가능하였다. 복합체의 XRD 상 분석 결과, CaCO3를 첨가하였을 때에는 모든 조성이 비정질을 나타내었고, $Al_2O_3$와 $1CaCO_3-1Al_2O_3$ 혼합물은 $Al_2O_3$ 결정상이 생성되었고, $CaAl_2O_4$ 화합물은 $CaAl_2Si_2O_8$의 hexagonal와 anorthite 결정상이 생성되었다. 따라서 CAS-10 (A, C-A, CA) 복합체는 $900^{\circ}C$에서 각각 유전율 ($\varepsilon_r$) 6.4, 6.9, 5.15 와 품질계수 ($Q^*f$) 2,400, 1,500, 3,000의 마이크로파 유전 특성을 나타내어 LTCC 기판 재료로 사용이 가능하며, 특히 $CaAl_2O_4$ 화합물을 사용하였을 때 가장 우수한 유전 특성을 나타내는 것을 확인하였다.

• Journal of Mushroom
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• v.20 no.3
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• pp.178-182
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• 2022

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.

• Korean Journal of Remote Sensing
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• v.36 no.5_2
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• pp.949-959
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• 2020

In this paper, as a prior study related to the generation of topographic information using the CAS500-1/2 satellite, we propose a method of extraction DSM for each 1:5000 scaled map in North Korea using KOMPSAT-3A strip images. This technique is designed to set the processing area by receiving shape file, only to generate output for every 1:5000 scaled map. In addition, dense point clouds and the DSM were extracted by applying MDR, a robust stereo image matching technique. Considering that the strip images are input in the units of scenes, we attempted to extract a DSM by processing and merging multiple image pairs in one 1:5000 map area. As a result, it was possible to confirm the generation of an integrated DSM with minimal separation at the junction, and as a result of the accuracy analysis, it was confirmed that the accuracy was within 5m compared to GCP.

• Proceedings of the KIEE Conference
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• 2003.07c
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• pp.1514-1516
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• 2003

NiCrFe 박막을 이용한 정밀급 박막 스트레인 게이지를 개발하였으며, 그 특성을 요약하면 다음과 같다. 스트레인한계: 5 % 이하, 저항: 350 ${\sim}$ 2,000 ${\Omega}$, 게이지 율: 2.1, 정도: 0.1 %, 피로한계 : $10^6$ at ${\pm}1500{\mu}{\varepsilon}$, 사용온도범위: $-75{\sim}150^{\circ}C$, 온도 출력: ${\pm}1{\mu}{\varepsilon}/^{\circ}C$, 게이지율변화 : +0.009%/$^{\circ}C$ 국산화된 박막 스트레인 게이지는 디지털 로드 셀 등에 적용할 수 있으며, 이 분야의 국내 기술력 향상에 이바지 할 것이다.

• Journal of Astronomy and Space Sciences
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• v.19 no.4
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• pp.263-272
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• 2002

A total of 616 observations (308 in B, 308 in V) to. V523 Cas was made on three nights from October 19 to 21 in 1999 using the 1.8m telescope with 2K CCD camera of the Bohyunsan Optical Astronomy Observatory of KAO. With our data we constructed the BV light curves and determined 4 times of minimum light. We also obtained physical parameters of the system by combined analysis of both light and radial velocity curves using the Wilson-Devinney code.

• BMB Reports
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• v.57 no.1
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• pp.60-65
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• 2024

The CRISPR-Cas9 system has significantly advanced regenerative medicine research by enabling genome editing in stem cells. Due to their desirable properties, mesenchymal stem cells (MSCs) have recently emerged as highly promising therapeutic agents, which properties include differentiation ability and cytokine production. While CRISPR-Cas9 technology is applied to develop MSC-based therapeutics, MSCs exhibit inefficient genome editing, and susceptibility to plasmid DNA. In this study, we compared and optimized plasmid DNA and RNP approaches for efficient genome engineering in MSCs. The RNP-mediated approach enabled genome editing with high indel frequency and low cytotoxicity in MSCs. By utilizing Cas9 RNPs, we successfully generated B2M-knockout MSCs, which reduced T-cell differentiation, and improved MSC survival. Furthermore, this approach enhanced the immunomodulatory effect of IFN-r priming. These findings indicate that the RNP-mediated engineering of MSC genomes can achieve high efficiency, and engineered MSCs offer potential as a promising therapeutic strategy.

• Proceedings of the Korean Geotechical Society Conference
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• 2003.03a
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• pp.375-382
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• 2003

This study, Using Pusan clay, examined a relationship between Dynamic and Static Liquid Tests. The Static Liquid Tests wow carried out based on BS and JIS. The results of the study are summarized as follows. (1) The result of the Dynamic Liquid Limit Test showed that the liquid limit values of air-dried soil were greater than those of oven-dried soil by 4%~15% (2) The liquid limit value of the Static Liquid Limit Test was greater than that of the Dynamic Liquid Limit Test by 2%~9%. The following equations show the relationship between the two values WL,Fall(JIS) = 0.961ㆍWL,Cas. + 4.209 WL,Fall(BS) = 0.969ㆍWL,Cas + 5.024 (3) The liquid limit value of BS was greater than that of JIS by 1%~6%. The following equation shows the relationship between the two values.

• Animal Bioscience
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• v.36 no.2_spc
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• pp.333-338
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• 2023

Genetic modification enables modification of target genes or genome structure in livestock and experimental animals. These technologies have not only advanced bioscience but also improved agricultural productivity. To introduce a foreign transgene, the piggyBac transposon element/transposase system could be used for production of transgenic animals and specific target protein-expressing animal cells. In addition, the clustered regularly interspaced short palindromic repeat-CRISPR associated protein 9 (CRISPR-Cas9) system have been utilized to generate chickens with knockout of G0/G1 switch gene 2 (G0S2) and myostatin, which are related to lipid deposition and muscle growth, respectively. These experimental chickens could be the invaluable genetic resources to investigate the regulatory pathways and mechanisms of improvement of economic traits such as fat quantity and growth. The gene-edited animals could also be applicable to the livestock industry.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.17 no.2
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• pp.80-85
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• 2005

This study, Using Pusan clay, examined a relationship between Dynamic and Static Liquid Tests. The Static Liquid Tests were carried out based on BS and JIS. The results of the study are summarized as follows. (1) The result of the Dynamic Liquid Limit Test showed that the liquid limit values of Natural condition soil were greater than those of oven-dried soil by $4\%\~15\%$. (2) The liquid limit value of the Static Liquid Limit Test was greater than that of the Dynamic Liquid Limit Test by $2\%\~9\%$. The following equations show the relationship between tile two values. WL, Fall(JIS)=0.961$\cdot$WL, Cas+4.209, WL, Fall(BS)=0.969$\cdot$WL, Cas+5.024, (3) The liquid limit value of BS was greater than that of JIS by $1\%\~6\%$. The following equation shows the relationship between the two values. WL, Fall(BS)=0.976WL, Fall(JIS)+2.638.