• Journal of International Academy of Physical Therapy Research
• /
• v.1 no.1
• /
• pp.45-51
• /
• 2010

2 week study was conducted to investigate the effects of Interferential Current(IC) and Kaltenborn-Evjenth Orthopedic Manual Therapy(KEOMT) on functional constipation. Interventions were applied to spinal segments between $T_9-L_2$ which provides innervations to the gastrointestinal tract. Subjects(n=24) were randomly allocated to two treatment groups: the IC group or the KEOMT group. Results for the IC therapy demonstrated significant decrease with the colonic transit time(CTT) as well as scores on the constipation assessment scale(CAS). The frequency of defecations per week had increased significantly(p<0.05). The KEOMT displayed decreased CTT in the left colon region. The scores on the CAS were reduced and frequency of defecations per week had increased significantly (p<0.05). This study not only showed that both modes of therapy improved symptoms of constipation, but also optimized gastrointesti. nal content movement, eventuating in a more normalized CTT. In conclusion, both the IC therapy and the Kaltenborn-Evjenth Orthopedic Manual Therapy have shown to be effective interventions for improving functional constipation.

• Journal of Astronomy and Space Sciences
• /
• v.14 no.2
• /
• pp.233-241
• /
• 1997

New UBV Photometric observations for a close binary RZ Cas were obtained at the Chungbuk National University Observatory for 42 nights from October 1991 to March 1993, and standardized UBV light curves were constructed. From the primary light curves, the eight minimum times were determined by using a new light element. We confirmed the primary minimum was totality and derived the average duration was 18.1 minutes corresponding to $0.^{p}01$. The photometric and spectroscopic solutions were calculated with the obtained light curves and the radial velocity curves of Duerbeck & Hanel(1979) by using Wilson-Devinney method. From these values, the absolute dimensions of this binary system were estimated.

• Environmental Mutagens and Carcinogens
• /
• v.24 no.1
• /
• pp.25-32
• /
• 2004

To validate and to estimate the chemical hazard playa very important role to environment and human health. The detection of many synthetic chemicals used in industry that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. In this resepct, the clastogenicity of 17 synthetic chemicals was evaluated with bone marrow micronucleus assay in mice. The positive control, mitomycin C (2 mg/kg, i.p.) revealed significant induction ratio of percentage of micronucleated polychromatic erythrocytes/1,000 polychromatic erythrocytes compared to solvent controls. The chemicals with relatively high $LD_{50}$ value such as allyl alcohol (CAS No. 107-18-6), 2,4-pentanedione (CAS No. 123-54-6) and 4-chloro-3,5-dimethylphenol (CAS No. 88-04-0) revealed no significant induction of micronucleated polychromatic erythrocytes in mice. From this results, 17 synthetic chemicals widely used in industry have revealed no significant micronucleus induction of clastogenicity in mice in this experiment.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.2
• /
• pp.605-621
• /
• 2019

The purpose of this study was to develop and validate the Korean version of the Classism Attitude Scale for college students (K-CAS) that measures stereotypes and prejudices about socioeconomic class(SES). In study 1, preliminary items were developed through reviewing classism related theories and literatures, analyzing qualitative data by using Consensus Qualitative Method-modified(CQR-M) and getting experts' consultation. With 440 college students data, exploratory factor analysis and reliability analysis were conducted with 14 items in study 1. In study 2, a confirmatory factor analysis and validity analysis were conducted with 400 college students. As a result, two factor structures, upper classism attitude and downward classism attitude, were confirmed. With the higher level of test-retest reliability, the criterion validity was confirmed by the significant correlations among age, subjective SES, the general belief in a just world, negative affects (depression and anxiety), hostility, and K-CAS. The incremental validity was also confirmed that K-CAS scores significantly accounted for 5% of total variation in scores of negative affects(depression and anxiety) in addition to the variables, such as age, subjective SES, house income, and the general belief in the just world. The result of this study is significant in that the developed and validated K-CAS in this study can be a useful tool for further research and prevention and intervention program development related to classism.

• The Bulletin of The Korean Astronomical Society
• /
• v.31 no.1
• /
• pp.35.2-35.2
• /
• 2006

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.5
• /
• pp.743-748
• /
• 2017

Objective: Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells. Methods: Chicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase. After co-transfection of the nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs), the GFP-positive cells were sorted out by fluorescence-activated cell sorting procedure. Results: Through the genotyping analysis of the knockout cells, the mutant induction efficiency was 100% in the targeted site. Number of the deleted nucleotides ranged from 2 to 39 nucleotide deletion. There was no phenotypic difference between regular cells and knockout cells. However, myostatin protein was not apparently detected in the knockout cells by Western blotting. Additionally, six off-target sites were predicted and analyzed but any non-specific mutation in the off-target sites was not observed. Conclusion: The knockout technical platform with the nickase and multiplex gRNAs can be efficiently and stablely applied to functional genomics study in poultry and finally adapted to generate the knockout poultry for agribio industry.

• Bulletin of the Korean Space Science Society
• /
• 1992.10a
• /
• pp.15-15
• /
• 1992

AQ Cassiopeiae (BD+61`0242, uv=10, Sp=B3+Bg) is a totally eclipsing binary system with the obital period of about 12 days. 71 was observed for 15 nights in 1985 with the1.8-m telescope at the DAO, employing a Reticon and a three-stage image tube attached to the spectrograph. And also, photometric observations of AQ Cas had been made inUBv for six years from 1982 to 1988 at Yonsei University Observatory(YUO). This work includes UBV observations obtained at YUO as a part of The Ten-Year Observing Program(1982-1992). Double lined radial, velocity curves and Ufv light curves of AQC as are constructed. The light curves and radial velocity curves show a strong evidence of circumstellar matter or mass stream. It is clear at the phases of just outside externaleclipse contacts, particularly at phase 0.8-0.9, shown in Figures 1 and 2. A solution by combining the radial velocity and photometric curves of the binary was obtained with the Wilson-Devinney Code. We found that the system is semi-detached with the coolcomponent filling its Roche lobe. The absolute dimensions of AQ Cas are calculated. The result shows that this system consists of two massive and subgiant stars.

• Journal of Environmental Impact Assessment
• /
• v.24 no.2
• /
• pp.134-153
• /
• 2015

When making urban planning, it is important to understand climate effect caused by urban structural changes. Seoul city applies UPIS(Urban Plan Information System) which provides information on urban planning scenario. Technology for analyzing climate effect resulted from urban planning needs to developed by linking urban planning scenario provided by UPIS and climate analysis model, CAS(Climate Analysis Seoul). CAS develops for analyzing urban climate conditions to provide realistic information considering local air temperature and wind flows. Quantitative analyses conducted by CAS for the production, transportation, and stagnation of cold air, wind flow and thermal conditions by incorporating GIS analysis on land cover and elevation and meteorological analysis from MetPhoMod(Meteorology and atmospheric Photochemistry Meso-scale model). In order to reflect land cover and elevation of the latest information, CAS used to highly accurate raster data (1m) sourced from LiDAR survey and KOMPSAT-2(KOrea Multi-Purpose SATellite) satellite image(4m). For more realistic representation of land surface characteristic, DSM(Digital Surface Model) and DTM(Digital Terrain Model) data used as an input data for CFD(Computational Fluid Dynamics) model. Eight inflow directions considered to investigate the change of flow pattern, wind speed according to reconstruction and change of thermal environment by connecting green area formation. Also, MetPhoMod in CAS data used to consider realistic weather condition. The result show that wind corridors change due to reconstruction. As a whole surface temperature around target area decreases due to connecting green area formation. CFD model coupled with CAS is possible to evaluate the wind corridor and heat environment before/after reconstruction and connecting green area formation. In This study, analysis of climate impact before and after created the green area, which is part of 'Connecting green network across the north and south in Seoul' plan, one of the '2020 Seoul master plan'.

• Environmental Analysis Health and Toxicology
• /
• v.18 no.2
• /
• pp.111-120
• /
• 2003

The validation of many synthetic chemicals that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. In this respect, the regulation and evaluation of the chemical hazard playa very important role to environment and human health. The clastogenicity of 17 synthetic chemicals was evaluated in Chinese hamster lung fibroblast cells in vitro. 2-Nitroaniline (CAS No. 88-74-4) induced chromosomal aberrations with statistical significance at the concentration of 86.3 ${\mu}$g/ml in the absence of metabolic activation system. 1-Chloroanthraquinone (CAS No. 82-44-0) which is one of the most cytotoxic chemical among 17 chemicals tested revealed no clastogenicity in the range of 0.8 ∼ 3.0 ${\mu}$g/ml both in the presence and absence of S-9 metabolic activation system. From the results of chromosomal aberration assay with 17 synthetic chemicals in Chinese hamster lung cells in vitro, 2-Nitroaniline (CAS No. 88-74-4) revealed weak positive clastogenic results in this study.

• Journal of Embryo Transfer
• /
• v.30 no.4
• /
• pp.299-303
• /
• 2015

KO mice provide an excellent tool to determine roles of specific genes in biomedical filed. Traditionally, knockout mice were generated by homologous recombination in embryonic stem cells. Recently, engineered nucleases, such as zinc finger nuclease, transcription activator-like effector nuclease and clustered regularly interspaced short palindromic repeats (CRISPR), were used to produce knockout mice. This new technology is useful because of high efficiency and ability to generate biallelic mutation in founder mice. Until now, most of knockout mice produced using engineered nucleases were C57BL/6 strain. In the present study we used CRISPR-Cas9 system to generate knockout mice in FVB strain. We designed and synthesized single guide RNA (sgRNA) of CRISPR system for targeting gene, Abtb2. Mouse zygote were obtained from superovulated FVB female mice at 8-10 weeks of age. The sgRNA was injected into pronuclear of the mouse zygote with recombinant Cas9 protein. The microinjected zygotes were cultured for an additional day and only cleaved embryos were selected. The selected embryos were surgically transferred to oviduct of surrogate mother and offsprings were obtained. Genomic DNA were isolated from the offsprings and the target sequence was amplified using PCR. In T7E1 assay, 46.7% among the offsprings were founded as mutants. The PCR products were purified and sequences were analyzed. Most of the mutations were founded as deletion of few sequences at the target site, however, not identical among the each offspring. In conclusion, we found that CRISPR system is very efficient to generate knockout mice in FVB strain.