• Development and Reproduction
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• v.20 no.4
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• pp.275-281
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• 2016

The purpose of this study was to investigate the improvement of growth in Israeli carp (Cyprinus carpio), and the cross experiment was carried out with two strains of Israeli carp. Four combinations of Israeli carp from Jeonbuk fisheries farm and Songpu mirror carp from Heilong Jiang, China (KK; Jeonbuk ♀ ${\times}$ Jeonbuk ♂, KC; Jeonbuk ♀ ${\times}$ China ♂, CC; China ♀ ${\times}$ China ♂ and CK; China ♀ ${\times}$ Jeonbuk ♂) were developed and reared. Body length, body weight and condition factor were determined at 20, 40, 60 and 170 days post-hatch (DPH). The results showed that there were differences in growth rate of the four groups. Body length of four groups were CK > CC > KC > KK and body weight were CC > CK > KC > KK at 170 DPH. The growth perfomance of four groups were statistically significant difference (P<0.05). During the rearing, CC group had longer length and higher weight at 170 DPH compared to other three groups and also condition factor was highest in the CC group, but there was no significant difference in a survival rate. These results indicated that the growth performance mainly depended upon brooder combination but survival rate could not significantly affect brooder.

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.268-275
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• 2004

To investigate the potential application of the single-cell gel electrophoresis (SCGE) assay to carp as an aquatic pollution monitoring technique, gill, liver, and blood cells were isolated from carp exposed to a direct-acting mutagen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), or indirect mutagen, $benzo[\alpha]pyrene$ $(B[\alpha]P)$, then the DNA strand breakage was analyzed using the assay. Based on testing 5 different cell isolation methods and 6 electrophoretic conditions, the optimized assay conditions were found to be cell isolation by filter pressing and electrophoresis at a lower voltage and longer running time (at 0.4 V/cm for 40 min). In preliminary experiments, gill and liver cells isolated from carp exposed to MNNG in vitro exhibited DNA damage signals even with 0.5 ppb exposure, which is a much higher dose than previously reported. In the gill cells isolated from carp exposed to 0.01-0.5 ppm MNNG in vivo, significant dose-and time-dependent increases were observed in the tail for 4 days. As such, the linear correlation between the relative damage index (RDI) values and time for each dose based on the initial 48-h exposure appeared to provide effective criteria for the genotoxicity monitoring of direct-acting mutagenic pollution. In contrast, the in vivo exposure of carp to 0.25-1.0 ppm of $B[\alpha]P$ for 7 days resulted in dose-and time-dependent responses in the liver cells, in which 24-h delayed responses for metabolizing activation and gradual repair after 48 h were also observed. Thus, the negative-sloped linear correlation between the RDI and time at each dose based on the initial 48 h appeared to provide more effective criteria for the genotoxicity monitoring of indirect mutagenic pollution.

• Fisheries and Aquatic Sciences
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• v.26 no.7
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• pp.437-446
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• 2023

Cyprinids are popular species for aquaculture worldwide, with Asia being a significant contributor to their production. In Korea, common carp (Cyprinus carpio), koi carp (Cyprinus rubrofuscus), and goldfish (Carassius auratus) are cultivated domestically and imported for ornamental or human consumption purposes. Among the viruses that infect cyprinids, cyprinid herpesvirus-2 (CyHV-2), koi herpesvirus (KHV, also known as cyprinid herpesvirus-3), and carp edema virus (CEV) are of particular concern as they cause substantial economic losses to the aquaculture industry. In this study, we investigated these viruses in both of domestic and imported cyprinids. Our results revealed that CyHV-2 was only detected in imported goldfish from Thailand. To further investigate the genetic characteristics of them, the marker A region was analyzed. Despite belonging to the same cluster with isolates from China, France, Poland, and Israel, CyHV-2 detected in this study showed distinct differences in their repetitive sequence sizes. Furthermore, two different forms of KHV/CEV coinfection were identified from domestic koi carp, both of which exhibited typical symptoms. Phylogenetic analysis showed that one KHV isolate (ScKc-2105-K) was of the Asian type and closely related to isolates from Japan, Indonesia, Belgium, Taiwan, and China. Two CEV isolates (ScKc-2105-CE and GhKc-2207-CE) be- longed to the IIa type and showed high similarity with isolates from the USA, France, and Korea. Notably, koi carp injected with cultured KHV (ScKc-2105-K) showed 78.0% cumulative mortality within 14 days post-injection (dpi). Our findings support the importance of regular surveillance of viral diseases in cyprinids.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.3
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• pp.154-160
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• 1989

To obtain basic data on food components of triploid fish, we undertook the analysis of free amino acid, nucleotide, total creatinine, betaine, trimethylamine oxide(TMAO) and their related compounds in diploid and triploid carps(Cyprinus carpio). The contents of total free amino acid and its related compounds in belly and dorsal muscles of triploid carp were 346.1mg/100g and 333.4mg/100g. Histidine occupied $45.1\%\;and\;46.9\%$ in belly and dorsal muscles, and followed by taurine, lysine and glycine in order. As for the compositions of nucleotide and its related compounds in those muscles of triploid carp, IMP were revealed $73.9\%\;and\;65.8\%$ of total nucleotide and its related compounds. The major component of the other organic base in those muscles of triploid carp was total creatinine, but betaine and TMAO were poor. The contents of taste compounds such as free amino acid, nucleotide, total creatinine, betaine, TMAO and their related compounds were less in triploid carp than in diploid carp of nonspawning season, while were more in triploid carp than in diploid carp of spawning season. Total amino acid contents were more in diploid carp of nonspawning season than in triploid carp, but mineral contents were more in triploid carp than in diploid carp of spawning season. Therefore, it is believed that triploid carp is very worthy for a tasty and nutritional food source.

• Analytical Science and Technology
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• v.27 no.6
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• pp.321-332
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• 2014

The accumulation levels of hexabromocyclododecanes (HBCDs) were measured in samples of muscle tissue from crucian carp (Carassius auratus) and also in samples of associated sediments. Samples were collected from 15 sites along the courses of four major rivers. Widespread contamination by HBCDs in this area was identified from the results of this investigation. HBCDs were found above the detection limit from all of the sampling sites. The ${\Sigma}HBCDs$ (sum of ${\alpha}$-, ${\beta}$-, and ${\gamma}$-HBCDs) levels ranged from 1.3 to 19 ng/g (lipid wt) in the crucian carp musscle tissue samples, and from 0.17 to 30 ng/g (dry wt) in the associated sediment samples. The predominance of ${\alpha}$-diastereomer in the muscle tissue of the carp and ${\gamma}$-diastereomer in the sediments was identified, accounting for 88% and 60% of the ${\Sigma}HBCDs$, respectively. The ${\Sigma}HBCDs$ levels in the muscle tissue samples of carp collected from the upstream sections of each river were much lower than those in the samples from carp in the downstream sections. In addition, the ${\Sigma}HBCDs$ levels in carp tissue samples from an isolated site were much lower than those in carp collected from the sites along the major rivers. This suggests that HBCD contamination in the rivers is likely to be sourced from the environment adjacent to them.

• Preventive Nutrition and Food Science
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• v.2 no.4
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• pp.321-327
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• 1997

High Temperature-cooking conditions of cultured fishes(loach, crucian carp, bastard halibut, and jacopever) were optimized by response surface methodology(RSM), and plastein products were prepared using enzymatic hydrolysis. Four models were proposed with regard to effects of time(t), temperature(T), and water/fish meat (w/f) ratio on the amount of 0.3M TCA soluble fractions. The model coefficients were ranged from p<0.0001 for jacopever to p<0.0433 for bastared halibut. Cooking conditions for 60% hydrolysis were optimized at 1) 14$0^{\circ}C$ except for crucian carp(136$^{\circ}C$); 2) 10.08 hours(loach), 7.25 hours(crucian carp), 9.85 hours(ba-stard harlibut), and 9.37 hours(iacopever); 3) 1:1(w/f) ratio except for the crucian carp(1.1:1). When protein hydrolyzates were employed for the plastein synthesis, optimum plastein-reaction conditions were determined to be pH 9.0 with chymotrypsin for the loach and crucian carp hydrolyzates, pH 9.0 with papain for the bastard halibut hydrolyzate, and pH 11.0 with trypsin for the jacopever hydrolyzate. Plastein reaction could be performed in water at concentration up to 20%(w/f).

• Journal of fish pathology
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• v.23 no.2
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• pp.165-176
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• 2010

In May 2007, mass mortality of Israeli carp, Cyprinus carpio L., was occured on a pond farm located in Jeollabukdo Province, Korea. The mortality rates reached up to 2% of the total fish in the farm per day. Typical clinical signs were abdominal distension, reddish foci on the skin, enteritis, liver congestion and enlarged spleen and kidney. On the basis of biochemical characteristics and 16S rDNA sequence, the causative bacteria isolated from affected carp were identified as Aeromonas veronii. Histologically, degeneration of hepatocytes and congestion in sinusoids were observed in the liver. Spleen showed hemorrhage and the destruction of the sheathed tissues. In kidney, necrotized renal tubules and glomerular destructions were observed. Intestinal tissues revealed necrotized and severe hemorrhage. Mass hemorrhage was observed in muscles. This is the first report that A. veronii caused mortality in cultured Israeli carp in Korea.

• Development and Reproduction
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• v.24 no.4
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• pp.277-285
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• 2020

The disease-causing koi herpes virus (KHV), also known as cyprinid herpesvirus-3 (CyHV3), causes mass mortality of koi and carp. Koi (Cyprinus carpio) is a host for KHV, one of 12 virus species in the Alloherpesviridae family. We examined the effects of KHV disease koi (KK), and on koi×red common carp (KR) and red common carp×koi (RK) cross, using a virus challenge test. The infected fish had clinical signs that included gill necrosis and skin lesions. The RK and KR were highly more resistant (cumulative mortality: RK; 6% and KR; 8%) to KHV infection than KK fish (cumulative mortality: 28%). KHV DNA was confirmed in the tissues of all dead fish in groups by use of polymerase chain reaction (PCR), and the presence of the KHV protein in kidney was confirmed by immunohistochemistry. Histological analysis showed severe gill lesions and fusion of the lamellae in KK fish, but less severe damage in RK fish. In immunohistochemistry analysis, the KHV protein localized in the cytoplasm of infected kidney cells of KK, but the cross groups had lower levels of KHV antigen. Our data indicate that the cross groups had increased resistance to KHV disease.

• Korean Journal of Environmental Agriculture
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• v.14 no.2
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• pp.163-170
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• 1995

This study was carried out to compare the acute toxicity(96hr) of 13 chemicals to carp (Cyprinus carpio L.) and israeli carp (Cyprinus israeli carpio L.) and the activities of acetylcholinesterase (AchE) and glutathione S-transferase (GST) in israeli carp exposed to five insecticides (diazinon, malathion, carbofuran, cartap, methomyl). $LC_{50}$ values of acute toxicity of the chemicals to israeli carp were endosulfan 0.0061ppm, captafol 0.041ppm, chlorothalonil 0.073ppm, butachlor 0.48ppm, captan 0.14ppm, carbofuran 1.13ppm, cartap 1.15ppm, diazinon 1.35ppm, nitrofen 3.72ppm, methomyl 4.39ppm, propanil 10.61ppm, malathion 11.78ppm and isoprothiolane 12.81ppm. The acute toxicity of endosulfan 0.0061ppm was 2100 times higher than that of isoprothiolane 12.81ppm. $LC_{50}$ values of acute toxicity of the chemicals to carp were endosulfan 0.0026ppm, captafol 0.062ppm, chlorothalonil 0.078ppm, captan 0.14ppm, and butachlor 0.47ppm, carbofuran 0.52ppm, nitrofen 0.58ppm, diazinon 0.81ppm, cartap 0.82ppm, methomyl 5.03ppm, propanil 10.67ppm, malathion 11.92ppm, and isoprothiolane 13.20ppm. The acute toxicity of endosulfan was 5,000 times higher than that of isoprothiolane. The toxicity of diazinon, carbofuran, cartap, endosulfan, and nitrofen to carp was approximately 2-6 times as high as that to israeli carp, but the toxicity of malathion, methomyl and captafol to israeli carp was slightly higher than that to carp. AchE activity was inhibited by 31% and 52% after 96hr’s exposure of israeli carp to diazinon and malathion respectively. GST activity in israeli carp was significantly induced by methomyl exposure for 96 hr.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.332-333
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• 2001

Genomic DNA was extracted from the blood of the freshwater crucian carp(Carassius carassius) from Kunsan in Korea, representing genetic similarity by polymerase chain reaction amplification of DNA as twelve of arbitrary primers. The electrophoretic analysis of polymerase chain reaction-random amplified polymorphic DNAs(PCR-RADP) products showed the high levels of similarity between different individuals in crucian carp.