• 한국응용과학기술학회지
• /
• 제32권4호
• /
• pp.758-766
• /
• 2015

Dextran is a generic term for a bacterial exopolysaccharide synthesized from sucrose and composed of chains of D-glucose units connected by ${\alpha}$-1,6-linkages by using dextransucrases. Dextran could be used as vicosifying, stabilizing, emulsifying, gelling, bulking, dietary fiber, prebiotics, and water holding agents. We isolated new strain capable of producing dextran from Korean traditional kimchi and identified as Leuconostoc sp. strain JYY4. Batch fermentation was conducted in bioreactor with a working volume of 3 L. The media was MMY and 15% (w/v) sucrose. Mineral medium consisted of $3.0g\;KH_2PO_4$, $0.01g\;FeSO_4$, $H_2O$, $0.01g\;MnSO_4$, $4H_2O$, $0.2g\;MgSO_4\;7H_2O$, 0.01 g NaCl, $0.05g\;CaCl_2$ per 1 liter deionized water. The pH of media was initially adjusted to 6.0. The inoculation rate was 1.0% (v/v) of the working volume. Temperature was maintained at $28^{\circ}C$. The agitation rate was 100 rpm. The production pattern of dextran was associated with the cell growth. After 24 hr dextran reached its highest concentration of 59.4 g/L. The sucrose was consumed completely after 40 hr. Growth reached stationery phase when sucrose became limiting, regardless of the presence of fructose or mannitol. When the specific growth rate was 0.54 hr-1, utilization averaged 5.8 g/L-hr. The yield and productivity of dextran were 80% and 2.0 g/L-hr, respectively. Dextrans produced by were separated to two different size by an alcohol fraction method. The size of high molecular weight dextran (45% alcohol, v/v), less soluble dextran, was between MW 500,000 and 2,000,000. Soluble dextran (55% alcohol, v/v) was between 70,000 and 150,000. The molecular weight average of total dextran (70% alcohol, v/v) was between 150,000 to 500,000. The enzymatic hydrolyzates of total dextran of ATCC 13146 showed branched dextrans by Penicillium dextranase contained of glucose, isomaltose, isomaltotriose, and isomaltooligosaccharides greater than DP4 (degree of polymerization) that had branch points. Compounds greater than DP4 were branched isomaltooligosaacharides. Hydrolysates by the Lipomyces dextranase produced the same composition of oligosaccharides as those by Penicillin dextranase.

• Tuberculosis and Respiratory Diseases
• /
• 제65권3호
• /
• pp.177-182
• /
• 2008

연구배경: Isoniazid (INH, H)는 4제 표준 항결핵치료의 근간이 되는 중요한 약제이지만 INH를 제외하여도 치료효과에 차이가 없다는 일부 보고들이 있다. 본 연구는 초기 결핵 생쥐 모델을 이용하여 항결핵 표준병합치료에서 INH의 유용성을 평가하였다. 방 법: 140마리의 C57BL/6 생쥐를 Glas-Col 장비를 이용하여 약 100개의 H37Rv가 호흡기로 감염되도록 하였다. 감염 4주 후부터 다양한 약제의 조합으로 4~8주간 치료를 시행한 후 감염 후 28주까지 주기적으로 각각 4마리에서 폐와 비장을 적출하여 결핵균 수를 측정하였다. 1군은 약물치료를 시행하지 않은 대조군이었으며, 2군은 INH, rifampicin (RMP, R), ethambutol (EMB, E), pyrazinamide (PZA, Z)로 4주 치료하였고(4HREZ), 3군은 1HREZ/3REZ, 4군은 4REZ, 5군은 4HREZ/4HRE, 6군은 1HREZ/3REZ/4RE, 7군은 4REZ/4RE이었다. 결 과: 4주 치료한 군(2~4군)은 치료종료 시점에 폐에서 균이 배양되지 않았으나 치료 종료 12주, 20주 시점에서 다시 균이 검출되었다. 8주 치료한 군(5~7군)은 모두 치료시작 1주까지는 균수가 증가하였다가 2주 후부터 감소하여 치료시작 4주 시점에는 모두 균이 배양되지 않았다. 이후 8주간의 치료를 종료하고 종료 후 16주간 경과 관찰할 때까지(감염 후 28주) 모두에서 균이 배양되지 않았다. 비장에서는 감염 4주 후부터 균이 배양되었다. INH를 전혀 사용하지 않은 7군에서는 치료시작 4주 시점부터 균이 배양되지 않고 이후 감염 28주 시점까지 지속적으로 균이 배양되지 않았다. 반면에 INH를 지속적으로 사용하거나 일시적으로 사용한 5, 6군에서는 치료시작 4주 이후시점에도 간헐적으로 균이 배양되었다. 결 론: 초기 결핵 생쥐 모델에서 INH의 제외는 표준요법의 치료효과에 영향을 미치지 않았다. 향후 만성 결핵생쥐 모델을 이용한 추후 연구가 필요하다.

• 한국지반환경공학회 논문집
• /
• 제7권4호
• /
• pp.15-24
• /
• 2006

원형 오픈 케이슨과 원형 수직구에 설치된 흙막이 벽에 작용하는 토압은, 벽체의 변위와 응력해방으로 인한 수평 및 연직 아칭효과로 인하여, 평면 변형조건에서의 옹벽에 작용하는 토압보다는 작은 토압이 발생한다. 원형벽체에 작용하는 토압분포를 조사하기 위해서, 건조한 모래지반에서 깊이에 따른 벽체변형이 균등한 조건의 모형실험을 실시하였다. 벽체 변위, 벽면 마찰, 벽체 형상비 등을 조절할 수 있는 모형 실험 장치가 개발되었고, 모형실험을 통하여 원통형 벽체에 작용하는 토압에 대한 다양한 인자의 영향이 분석되었다.

• 동의생리병리학회지
• /
• 제27권1호
• /
• pp.107-111
• /
• 2013

To investigate the possible usage as probiotics, we isolated and identified one strain of bacillus from Korean traditional food, Jeotgal. The isolate was identified as Bacillus megaterium KS12 by examining its biochemical properties and 16S rRNA sequence analysis (99% similarity). B. megaterium KS12 exhibited high viability in artificial gastric juice for 3 h and in artificial bile salts for 24 h, about 14.4 and 14%, respectively. The herbal digestive medicine containing the isolate showed high starch hydrolytic activity and proteolytic activities (about 6-fold) compared to traditional herbal digestive medicine. The viable cells in the herbal digestive medicine containing the isolate were higher than those of traditional herbal digestive medicine.

• 여성건강간호학회지
• /
• 제10권2호
• /
• pp.119-127
• /
• 2004

Purpose: A Phenomenological study was conducted to investigate the experiences of infertile women who had an unsuccessful in vitro fertilization(IVF) attempt and the structure of these experiences was identified in a socio-cultural context. Method: In-depth interviews were done with five participants from Nov. 1997 to Feb. 1998. Data was recorded and analyzed by Colazzi's method. Result: Significant statements were extracted from the data resulting in 4 themes and 11 concepts. The concepts were: 'lack of information', 'unable to anticipate the outcome', 'reaffirmation of need', 'financial strain', 'emotional suppression', 'change of social relationships', 'physical fatigue', 'blaming oneself or God', 'trying different ways', 'famous place shopping', and 'praying to God'. The significant themes of the participants' experiences with IVF emerged as: "uncertainty", "burden", "blaming" and "vigorous desire". Conclusion: The results indicate that the care of infertile women with an unsuccessful IVF attempt should be based on a deep understanding of their personal experiences. Also, information on alternative choices should be offered and supported.

• Safety and Health at Work
• /
• 제6권3호
• /
• pp.233-239
• /
• 2015

Background: The Job Content Questionnaire (JCQ), which is based on the Demand-Control-Support model, is designed to measure the psychosocial characteristics of the respondent's work, and has been identified to predict health and psychological outcomes. The purpose of this study was to investigate the psychometric properties of this instrument and the subsequent adaptation of its scales to the population of Greek health workers. Methods: The Greek version of the JCQ was developed by using forward- and back-translation in accordance with the JCQ policy. The reliability and validity of the measure were investigated in a sample of health workers working in a hospital in Athens, Greece. The internal consistency of the scales was examined based on Cronbach ${\alpha}$ coefficients, and the validity was evaluated subjecting the items of the three main scales of the JCQ (decision latitude, psychological job demands, and social support) to exploratory and confirmatory factor analysis. Results: The reliability of the scales was found to be acceptable for all the scales, except for the skill discretion subscale. Confirmatory factor analysis confirmed a slightly modified version of the original construct including several items to more than one factor. Conclusion: Our findings suggest that the Greek JCQ is reliable and valid for investigating psychosocial job characteristics among Greek health workers.

• Journal of Microbiology and Biotechnology
• /
• 제15권1호
• /
• pp.188-191
• /
• 2005

Abstract Cyclosporins are a family of clinically-important immunosuppressive cyclic peptides produced by Tolypocladium inflatum. The structural modification of cyclosporins via hydroxylation at various positions of N-methyl leucines in cyclosporin A leads to a dramatic change of their bioactive spectra. Among over 100 soil actinomycetes screened, two actinomycetes species, Sebekia benihana and Pseudonocardia autotrophica, were identified to contain superior cyclosporin A hydroxylation activities. A HPLC-based cyclosporin A hydroxylation assay revealed that each strain possesses distinctive hydroxylation specificity and regiospecificity; mono-hydroxylation at the 4th N-methyl leucine of cyclosporin A by S. benihana, and di-hydroxylations at both 4th and 9th N-methyl leucines of cyclosporin A by P. autotrophica. The conversion yields for cyclosporin A hydroxylation by both S. benihana and P. autotrophica were significantly improved from less than 10% and 18% up to 58% and 45%, respectively, in the optimized culture containing molybdenum with 0.05 g/l of cyclosporin A concentration. An ancymidol-specific inhibition of cyclosporin hydroxylation also suggested that the regiospecific cyclosporin hydroxylation might be catalyzed by a putative cytochrome P450 mono-oxygenase enzyme.

• Smart Structures and Systems
• /
• 제5권4호
• /
• pp.381-395
• /
• 2009

The paper presents a case study in which the structural condition assessment of the East Bay bridge in Gibsonton, Florida is evaluated with the help of remote health monitoring techniques. The bridge is a four-span, continuous, deck-type reinforced concrete structure supported on prestressed pile bents, and is instrumented with smart Fiber Optic Sensors. The sensors used for remote health monitoring are the newly emerged Fabry-Perot (FP), and are both surface-mounted and embedded in the deck. The sensing system can be accessed remotely through fast Digital Subscriber Lines (DSL), which permits the evaluation of the bridge behavior under live traffic loads. The bridge was open to traffic since March 2005, and the collected structural data have been continuously analyzed since. The data revealed an increase in strain readings, which suggests a progression in damage. Recent visual observations also indicated the presence of longitudinal cracks along the bridge length. After the formation of these cracks, the sensors readings were analyzed and used to extrapolate the values of the maximum stresses at the crack location. The data obtained were also compared to initial design values of the bridge under factored gravity and live loads. The study showed that the proposed structural health monitoring technique proved to provide an efficient mean for condition assessment of bridge structures providing it is implemented and analyzed with care.

• Safety and Health at Work
• /
• 제11권1호
• /
• pp.80-87
• /
• 2020

Background: Residential and commercial cleaning is a part of our daily routine to maintain sanitation around the environment. Health care of professionals involved in such cleaning activities has become a major concern all over the world. The present study investigates the risk of musculoskeletal disorders in professional cleaners involved in floor mopping tasks. Methods: A cross-sectional study was performed on 132 mopping professionals using a modified Nordic questionnaire. The Pearson correlation test was implemented to study the association of perceived pain with work experience. The muscle strain and postural risk were evaluated by means of three-channel electromyography and real-time motion capture respectively of 15 professionals during floor mopping. Results: Regarding musculoskeletal injuries, risk was reported majorly in the right hand, lower back, left wrist, right shoulder, left biceps, and right wrist of the workers. Work experience had a low negative association with MSDs in the left wrist, right wrist, right elbow, lower back, and right lower arm (p < 0.01). Surface EMG showed occurrence of higher muscle activity in upper trapezius and biceps brachii (BB) muscles of the dominant hand and flexor carpi radialis and BB muscles of the nondominant hand positioned at the upper and lower portion of the mop rod, respectively. Conclusion: Ergonomic mediations should be executed to lessen the observed risk of musculoskeletal injuries in this professional group of workers.

• Computers and Concrete
• /
• 제6권4호
• /
• pp.319-341
• /
• 2009

A new formulation based on lumped plasticity and inelastic hinges is presented in this paper for nonlinear analysis of Reinforced Concrete (RC) frame structures. Inelastic hinge behaviour is described using the principles of Continuum Damage Mechanics (CDM). Member formulation contains provisions to model stiffness degradation due to cracking of concrete and yielding of reinforcing steel. Depending on its nature, cracking is classified as concentrated or distributed. Concentrated cracking is accounted through a damage variable and its growth is defined based on strain energy principles. Presence of distributed flexural cracks in a member is taken care of by modelling it as non-prismatic. Plasticity theory supported by effective stress concept of CDM is applied to describe the post-yield response. Nonlinear quasi-static analysis is carried out on a RC column and a wide two-storey RC frame to verify the formulation. The column is subjected to constant axial load and monotonic lateral load while the frame is subjected to only lateral load. Computed results are compared with those due to experiments or other numerical methods to validate the performance of the formulation and also to highlight the contribution of distributed cracking on global response.