• Microbiology and Biotechnology Letters
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• v.28 no.6
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• pp.349-354
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• 2000

Culture conditions and nitrogen sources were optimized for production of erythritol, a natural sweetener, by Candida magnoliae M26. The optimal culture conditions were found to be culture temperature of $28^{\circ}C$, initial pH of 7, aeration of 1 vvm and agitation speed of 500 rpm in a 2.5 1 jar-fermentor. Glucose was chosen as the best carbon cource bsed on cell growth and erythritol productivity. Kight steep water(LSW) and corn steep liquor (CSL) which are by-products in starch processing from corn were tested as a nitrogen source substitute for yeast extract. The use of either LSW or CSL did not change the fermentation performance. The experimental results using LSW and CSL showed 1.5 times higher in cell growth and almost the same value in erythritol productivity com-pared with the control fermentation using yeast extract as a nitrogen source. These results suggested that either LSW of CSL could be used as a nitrogen source in a large-scale fermentation for erythritol production.

• KSBB Journal
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• v.10 no.2
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• pp.212-220
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• 1995

An obligatory type II methanotroph Methylosinus trichosporium OB3b was cultivated on methanol as a sole carbon and energy source. The effects of methanol concentration, pH, temperature, nitrogen source and phosphate concentration on cell growth were investigated and the results were compared with the growth on methane, which had been studied previously. When $(NH_4)_2SO_4$ was used as a nitrogen source, the maximal specific growth rate (${\mu}max$) on methanol was $0.20hr^{-1}$ and the carbon conversion efficiency(CCE) was 43%. In comparison, on methane, ${\mu}max$ and CCE were $0.08hr^{-1}$ and 32%, respectively. Ammonia was found to be a better nitrogen source for methanol-growing cells. Cell yield on nitrogen (YX/N) was the same regardless of nitrogen source as 7.14g dry cells/g N, but the yield on methanol(YX/N) was higher with ammonia(0.8g dry cells/g MeOH) than with nitrate(0.64g dry cells/g MeOH). Optimal pH and temperature were 7.0 and $30^{\circ}C$, respectively. Methanol inhibition on cell growth was observed at above 0.5%(v/v). Inhibition by phosphate was observed at above 60mM, although the inhibition on methanol dehydrogenase activity started at a much lower level of 20mM. Based on the experimental findings, the cellular physiology of M. trichosporium OB3b growing on the two closely-related carbon sources were discussed extensively.

• Journal of Mushroom
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• v.3 no.2
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• pp.75-78
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• 2005

The results of studies for determining the nutrients sources of mycelial growth for optimal condition of Pleurotus cornucopiae are as follows; 1) Optimal carbon source of mycelial growth of Pleurotus cornucopiae is maltose(357mg/25ml/15d); 2) Optimal nitrogen source of mycelial growth of Pleurotus cornucopiae is peptone(347mg/25ml/15d); 3) Optimal organic acid source of mycelial growth of Pleurotus cornucopiae is glutamic acid(389mg/25ml/15d); 4) Optimal vitamin source of mycelial growth of Pleurotus cornucopiae is biotin(399mg/15d); and 5) Optimal C/N ratio for mycelial growth for determining the condition of Pleurotus cornucopiae is 40(398mg/15d).

• Advances in nano research
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• v.9 no.1
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• pp.25-32
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• 2020

In this work, we report the use of caffeine as an alternative source of nitrogen to successfully dope graphene (quaternary 400.6 eV and pyridinic at 398 eV according XPS), as well as the growth of silver nanowires (in-situ) in the surface of nitrogen doped graphene (NG) sheets. We used the improved graphene oxide method (IGO), chemical reduction of graphene oxide (GOx), and impregnation with caffeine as source of nitrogen for doping and subsequently, silver nanowires (NW) grow in the surface by the reduction of silver salts in the presence of NG, achieving a numerous of growth of NW in the graphene sheets. As supporting experimental evidence, the samples were analyzed using conventional characterization techniques: SEM-EDX, XRD, FT-IR, micro RAMAN, TEM, and XPS.

• KSBB Journal
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• v.18 no.6
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• pp.473-478
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• 2003

The purpose of this study is to look for the optimal conditions of methane production. The conditions tested for methane production enhancement were temperature, pH, carbon source, nitrogen source, and inhibitor which can affects methane production. As a result, optimal conditions for methane production were 30$^{\circ}C$, neutral pH, methanol as a carbon source, NH$_4$Cl as a nitrogen source. 2-Bromoethanesulfonic acid was used as an inhibitor which can affects methane production. Existence in broth less than 10mM, inhibited methane production. Organic acid measurements revealed that formic acid exists in broth as majority.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.1
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• pp.37-48
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• 2004

The purpose of this study was to evaluate adaptability of external carbon source using GPS-X program in pilot plant composed with 2-stage denitrification process. The result from analysis of pilot plant operation and GPS-X simulation showed that effluent concentration could be simulated similarly by modifying operation conditions, such as DO concentration, C/N ratio and other calibrated parameter. In order to satisfy the standard of the effluent water quality on T-N of 20mg/L, it required approximately 3.1 of C/N ratio and 50% of nitrogen removal efficiency when influent T-N is 36.9mg/L. To maintain the stable water quality of the receiving water, the effluent T-N concentration should be less than 10-15mg/L and the appropriate C/N ratio to remove nitrogen was 4.27-6.82. The analysis of sensitivity to kinetic coefficient and reaction constant showed that $Y_H$ and ${\mu}_{mAUT}$ were most sensitive to nitrate and ammonia nitrogen, relatively and sensitivity coefficient of their were 1.32, 1.98. It was concluded that as $Y_H$ decreased and ${\mu}_{mAUT}$ increased, the reaction rates of denitrification and nitrification increased and the removal efficiencies of $NO_3{^-}-N$ and $NH_4{^+}-N$ improved.

• Journal of Microbiology and Biotechnology
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• v.22 no.9
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• pp.1230-1236
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• 2012

Fish meal grades SL1 and SL2 from Sardine (Sardinella longiceps) and NJ from Pink Perch (Nemipterus japonicas) were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by Bacillus pumilus MTCC 7514. The analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. Protease production increased significantly (4,914 U/ml) in medium containing only fish meal, compared with the basal medium (2,646 U/ml). However, the elimination of inorganic salts from media reduced the protease productivity. In addition, all the three grades of fish meal yielded almost the same amounts of protease when employed as the sole source of carbon and nitrogen. Nevertheless, the best results were observed in fish meal SL1 medium. Furthermore, protease production was enhanced to 6,966 U/ml and 7,047 U/ml on scaling up from flask (4,914 U/ml) to 3.7 and 20 L fermenters, respectively, using fish meal (10 g/l). Similarly, the corresponding improvement in productivities over flask (102.38 U/ml/h) was 193.5 and 195.75 U/ml/h in 3.7 and 20 L fermenters, respectively. The crude protease was found to have dehairing ability in leather processing, which is bound to have great environmental benefits.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.326-336
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• 2016

Saccharomyces cerevisiae is one of the most employed cell factories for the production of bioproducts. Although monomeric hexose sugars constitute the preferential carbon source, this yeast can grow on a wide variety of nitrogen sources that are catabolized through central nitrogen metabolism (CNM). To evaluate the effects of internal perturbations on nitrogen utilization, we characterized strains deleted or overexpressed in GLT1, encoding for one of the key enzymes of the CNM node, the glutamate synthase. These strains, together with the parental strain as control, have been cultivated in minimal medium formulated with ammonium sulfate, glutamate, or glutamine as nitrogen source. Growth kinetics, together with the determination of protein content, viability, and reactive oxygen species (ROS) accumulation at the single cell level, revealed that GLT1 modulations do not significantly influence the cellular physiology, whereas the nitrogen source does. As important exceptions, GLT1 deletion negatively affected the scavenging activity of glutamate against ROS accumulation, when cells were treated with H₂O₂, whereas Glt1p overproduction led to lower viability in glutamine medium. Overall, this confirms the robustness of the CNM node against internal perturbations, but, at the same time, highlights its plasticity in respect to the environment. Considering that side-stream protein-rich waste materials are emerging as substrates to be used in an integrated biorefinery, these results underline the importance of preliminarily evaluating the best nitrogen source not only for media formulation, but also for the overall economics of the process.

• Journal of Korean Society on Water Environment
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• v.39 no.5
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• pp.390-395
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• 2023

The feasibility of applying the shortcut nitrogen removal process to treat livestock wastewater on individual farms was examined, and appropriate operating parameters were established. As a result,, it was determined that the nitrification reaction was carried out under 550 mg/L of ammonium nitrogen concentration, but it was less effective under conditions of high ammonia concentration. Consequently, it was confirmed that a partial injection of inflow water was necessary to minimize the effects of ammonia toxicity. Following the sequential batch reactor (SBR) operation results, it was difficult to achieve the effluent quality standard without an external carbon source. Also, selection of the appropriate hydraulic retention time was critical for the optimal SBR operation. Following the livestock farm application, with external carbon source injecting, the total nitrogen concentration in the effluent was 85.1 mg/L. This result revealed that the standard could be accomplished through a single treatment on individual livestock farms. The ratio of nitrite nitrogen to ammonia nitrogen in the effluent was verified to be suitable for implementing the anammox process with a 10 days of hydraulic retention time. This study demonstrated the potential applicability of process in the future. However, in order to apply to livestock farms, managing variations in wastewater load across individual farms and addressing reduced nitrogen oxidation efficiency during the winter season are crucial.

• ALGAE
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• v.28 no.1
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• pp.101-109
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• 2013

Some Chlorella species grow heterotrophically with organic substrate in dark condition. However, heterotrophic Chlorella species are limited and their optimum culture conditions are not fully known. In this study, three heterotrophic Chlorella species, two strains (C4-3 and C4-4) of C. vulgaris and one Chlorella sp. (C4-8) were examined on optimum culture conditions such as carbon source, temperature, and concentrations of nitrogen and phosphorus in Jaworski's medium (JM). And the growth and fatty acid composition of Chlorella were analyzed. For three heterotrophic Chlorella species, glucose (1-2%) as a carbon source only increased the growth and the range of optimum culture temperature was $26-28^{\circ}C$. Doubled concentrations of the nitrogen or phosphorus in JM medium also improved the growth of Chlorella. Chlorella cultured heterotrophically showed significantly higher growth rate and bigger cell size than those autotrophically did. C. vulgaris (C4-3) cultured heterotrophically showed the highest biomass in dry weight ($0.8g\;L^{-1}$) among three species. With respect to fatty acid composition, the contents of C16:0 and n-3 highly unsaturated fatty acid (HUFA) were significantly higher in autotrophic Chlorella than in heterotrophic one and those of total lipid were not different between different concentrations of nitrogen and phosphorus in JM medium. Among three Chlorella species in this study, C. vulgaris (C4-3) appeared to be the most ideal heterotrophic Chlorella species for industrial application since it had a high biomass and lipid content.