• Journal of environmental and Sanitary engineering
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• v.7 no.1
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• pp.45-56
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• 1992

The Anaerbic Anoxic/oxic process is one of the biological treatment methods to remove nitrogen and phosphorus effectively which are nutritional elements for eutrophication. Supernatant of primary sediment of Anaerobic digester is used as a carbon source instead of methanol methanol supply in usual A$_{2}$/O process. The efficiency of the following treatment processes are as follow : 1) Changing recycle ratio in the usual A$_{2}$/O process without the stage of Anaerobic digester. 2) Changing recycle ratio in the usual A$_{2}$/O process with the supernatant supply of the Anaerobic digester. In the result of comparison, changing recycle ratio is almost no effect in the removal of phosphorus, however the effect of removal in nitrogenous substance are remarkable, and the effect of Anaerobic digester is not as effective as expected because the BOD removed in the digester partly, the rate of phosphorus to the BOD exceed pertinent range.

• Environmental Engineering Research
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• v.20 no.3
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• pp.246-253
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• 2015

The effects of carbon-to-nitrogen (C/N) ratio in simulated rice straw hydrolysates using glucose and ammonium chloride on polyhydroxyalkanoates (PHA) accumulation by Cupriavidus necator was investigated. In general, PHA accumulation rate was higher under higher degrees of N-deficient conditions (e.g., C/N ratio of 360:1) than lower degrees of N-deficient conditions (e.g., C/N ratio of 3.6:1 and 36:1). Also, the most PHA accumulation was observed during the first 12 h after the PHA accumulation initiation. This study showed that the similar PHA accumulation could be achieved by using different accumulation periods depending on C/N ratios. N source presence was important for new cell production, supported by approximately ten times greater PHA accumulation under the N-deficient condition ($NH_4Cl$ 0.01 g/L) than the N-free (without $NH_4Cl$) condition after 96 h. C/N ratio of the rice straw hydrolysate was approximately 160:1, based on the glucose content, and this accumulated $0.36{\pm}0.0033g/L$ PHA with PHA content of $21{\pm}3.1%$ after 12 h. Since external C or N source addition for C/N ratio adjustment increases production cost, an appropriate accumulation period may be used for PHA accumulation from organic wastes, based on the PHA accumulation patterns observed at various C/N ratios and C and N concentrations.

• Journal of Korean Society for Atmospheric Environment
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• v.20 no.6
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• pp.749-758
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• 2004

Nitrous oxide ($N_2$O) has been known as an important trace gas due to the greenhouse gas and the major source of stratospheric oxide of nitrogen (NO). Soil is the major source of $N_2$O in nature. The physicochemical characteristics of soils affect the emission of $N_2$O from soil. These physicochemical parameters are soil moisture, soil temperature, and soil N content. Since these parameters are correlated to the flux of $N_2$O from soil individually and compositely, there still remain many unknowns in the mechanism to produce $N_2$O in soil and the roles of such physicochemical parameters which affect the soil $N_2$O emission. Soil $N_2$O fluxes were measured at different levels in water filled pore space (WFPS), soil temperature and soil N contents from the same amounts of soils which were sampled from agriculturally managed upland field in a depth of ～30 cm at Kunsan. The soil $N_2$O flux measurements were conducted in a laboratory with a closed flux chamber system. The optimum soil moisture and soil temperature were observed at 60% of WFPS and ～13$^{\circ}C$. The soil $N_2$O flux increased as soil N contents increases during the whole experimental hours (up to 48 hours). However, average $N_2$O flux decreased after ～30 hours when organic carbon was mixed with nitrogen in the sample soils. It is suggested that organic carbon could be important for the emission of $N_2$O, and that the ratio of N to C needs to be identified in the process of $N_2$O soil emission.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.5
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• pp.493-505
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• 2020

Food web structures in the lower trophic levels of the seas around the Korean peninsula were investigated in August 2019 using stable isotopes. There were variable ratios of the carbon (-26.18 ~ -20.61 ‰) and nitrogen stable (5.36 ~ 15.20 ‰) isotopes in the particulate organic matter (POM). Most of the organisms ingested micro-POM as a major food source, but this varied spatially. The chaetognaths (3.40 ± 0.61) occupied the highest trophic level. The isotope mixing model showed that the proportions (13 ~ 51 %) of some organisms (i.e., copepods and euphausiids) reflected the relative contributions as major food sources for chaetognaths at each site.

• KSBB Journal
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• v.11 no.5
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• pp.613-621
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• 1996

The effects of important medium components such as carbon, nitrogen sources and amino acids on the production of cyclosporin A(CyA) were investigated in an immobilized fungal cell fermentation using Tolypocladium inflatum. As carbon sources in the synthetic medium, fructose and maltose stimulated CyA production remarkably compared to glucose when ammonium sulfate was supplemented as a nitrogen source. In the absence of ammonium sulfate in the medium, however, CyA biosynthesis was reduced considerably without regard to C-sources tested. Ammonium sulfate was found to be the best N-source, and also ammonium phosphate and ammonium citrate showed some positive effects on CyA production. Optimum concentration of ammonium sulfate was 10g/L, and supplementation of ammonium sulfate at the start of fermentation was found to be the most efficacious for maximal production of CyA. Among the constituent amino acids of cyclic peptide, CyA, L-valine had the most significant effect on the biosynthesis of CyA, and maximum CyA production was observed when 10 g/L of L-valine was initially added.

• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.123-127
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• 2004

Pseudomonas sp. MBEL21 was newly isolated from soil samples and found to accumulate medium-chain-length Polyhydroxyalkanoates(MCL-PHAs) using oleic acid as a sole carbon source. Among the various nutrient limiting conditions examined, including nitrogen, sulfur and phosphorus, only phosphorus limitation supported the accumulation of MCL-PHAs up to 15 wt％ of dry cell weight in flask cultures. MCL-PHAs produced by Pseudomonas sp. MBEL21 was mainly composed of 3-hydroxy-5-cis-tetradecenoate. Fed-batch culture of Pseudomonas sp. MBEL21 by novel feeding strategies based on cell growth charcteristics was carried out under phosphorus limitation using oleic acid as a sole carbon source. The final cell concentration and PHA content of 82 g/L and 28 wt％, respectively, were obtained. Furthermore, PHA consisted of MCL-hydroxyalkanoates and 3-hydroxybutyrate could be produced using olive oil as a sole carbon source.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.340-342
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• 2005

An agar degrading bacterium, This strain was isolated from sea water in Jeju. The strain is Gram-negative, rod and strictly aerobe for growth. The identification bases on the 16S rRNA gene sequencing showed that the strain was closely related to the genus Agariovarans sp. and named Agariovorans sp. JA1. The strain grew on agar as a sole carbon and energy source and produced an extra cellular agarase. For the increase of agarase productivity, 0.5% agar, yeast extract and $NaNO_3$ were used as carbon, organic and inorganic nitrogen source, respectively. For effective production of agarase and growth, the pH, temperature and NaCl concentration were was pH 7, $25^{\circ}C$ $^{\sim}$ $30^{\circ}C$ and 2%, respectively.

• Journal of Environmental Health Sciences
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• v.32 no.5 s.92
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• pp.469-477
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• 2006

When denitrification was connected with a single stream process by using biofilter and sulfur-limestone, it was found that such connection enabled highly efficient nitrification without special unit operation of microorganisms or injection of external carbon sources which is being shown in general biological treatment processes. It was observed that in the trickling filter bed, decomposition of organic substances and highly efficient nitrification by both the forced pressure feed trickling and the air fan were simultaneously done. In the denitrification tank where sulfur-limestone was mixed at a certain ratio, limestone was used by autotrophic microorganisms as a source of supply for alkalinity, and nitrate $NO_{3}^{-}$-N was denitrified into nitrogen gas. And in the sulfur-limestone autotrophic denitrification, $NO_{3}^{-}-N\;or\;NO_{2}^{-}-N$ was denitrified as a sulfur compound in reduction state was oxidized into a final output of $SO_{4}^{-2}$. The mean concentration of the discharge water was 8.6 mg/l for T-N and 0.8 mg/l for T-P, respectively, and their mean treatment efficiency was 79.2% and 80.8%, respectively. Implementing highly efficient denitrification without injection of an external organic carbon source or internal return, it is concluded that the proposed process is suitable for a sewerage in a small village with the merits of low power consumption and easy maintenance.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.58-58
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• 2002

This study was excuted to decide the physiological characteristics of Pleurotus Ferulae Lanzi. P. Ferulae Lanzi. was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of P. Ferulae Lanzi. was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 25~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 5.0~6.0. The required carbon and nitrogen source for mycelial growth of P. Ferulae Lanzi. was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was starch or maltose. And the nitrogen source for pertinent mycelial growth was yeast extract.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.59-59
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• 2002

This study was excuted to decide the physiological characteristics of Lentinus lepides. L. lepides was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of Lentinus lepides was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 22~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 3.0~4.0. The required carbon and nitrogen source for mycelial growth of Lentinus lepides was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was maltose. And the nitrogen source for pertinent mycelial growth was peptone.