• 한국미생물·생명공학회지
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• 제29권1호
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• pp.43-49
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• 2001

Indole and its derivatives form a class of toxic recalcitrant environmental pollutants, Abacte-rium, strain KL-9 was isolated from soil with indole as a sole source of carbon and nitrogen. KL-9 was identified as Acinetobacter sp. on the basis of 16 S rRNA gene sequence, fatty acid and quinone compositions. This identification was also confirmed by the ability of carbon source utilization and other biochemical tests. The growth of Acinetobacter sp. KL-9 was fastest with 0.3mg/ml of indole as was inhibited by higher than 0.5mg/ml of indole in the medium, KL-9 with indole also produced indigo. The formation of indigo was stimulated inthe presence of glucose, which is not a growth-suppoting carbon source for KL-9. Additional biotransformation evidence showed that anthranilate is an intermediate for the degradation of indole KL-9.

• 미생물학회지
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• 제29권2호
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• pp.130-135
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• 1991

Trimorphomyces papilionaceus dikaryon 으로부터 자영적인 돌연변이에 의해 분리한 이배체를 대상으로 생장속도, U. V. 광 조사 후의 생존율, U. V. 분광광도계와 Hoechst 33258 핵염색에 의한 DNA 함량등을 monokaryon 및 dikaryon 과 비교하여 결정하였다. 이배체는 dikaryon 핵의 DNA 양과 비슷하였고 monokaryon 핵의 DNA 양의 2배 정도가 되었다. Glyoxalase II 의 band 양상에서 monokayon은 한 개의 isozyme band 만을 보인 반면, 이배체와 dikaryon은 같은 위치에서 두개의 isozyme band 가 관찰되었다. 이원전개한 전기영동실험에서 dikaryon 특이 단백질은 분자량이 66.000 보다 컸으며 분자량 24,000-29,000 사이에서 이배체 특이 단백질이 존재한다.

• Ocean and Polar Research
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• 제45권3호
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• pp.103-111
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• 2023

To evaluate the impact of effluents from land-based fish farms on the coastal ocean of Wando, Korea, we analyzed inorganic nutrients, particulate organic carbon (POC), dissolved organic carbon (DOC), and colored dissolved organic matter (CDOM) in the effluent and influent of land-based fish farms during the summer (July) of 2021. The average concentrations of nutrients (Dissolved inorganic nitrogen, phosphorus, and silicate; DIN, DIP, and DSi, respectively) in the effluents of this study area were 17±3.7 μM, 1.4±0.7 μM, and 14±1.6 μM, respectively. The average concentrations of POC and DOC were 37±22 μM and 81±13 μM, respectively, with POC accounting for about 30% for total organic carbon in effluents. The Reduced Dissolved Inorganic Nitrogen/Total Dissolved Inorganic Nitrogen ratio (0.7), potential short-period index, indicates that the discharge of nutrients excreted by the fish and unconsumed feed into coastal water results in such nutrients being deposited and accumulated in the sediment. Subsequently, this continuous accumulation triggers the release of ammonium ions during organic matter decomposition, and the ammonium-enriched waters that encroach on fish farms as influent seem to be due to the diffusion of high concentrations of ammonium from bottom sediment. Furthermore, we used fluorescence indices to examine the characteristics of organic matter sources, obtaining mean values of 1.54±0.19, 1.06±0.06, and 1.56±0.06 for the humification index, biological index, and fluorescence index, respectively, in the effluent. These results indicate that the organic matters had an autochthonous origin that resulted from microbial decomposition, and such organic matters were rapidly generated and removed by biological activity, likely supplied from the sediment. Our results suggest that the effluent from land-based fish farms could be a potential source of deoxygenation occurrence in coastal areas.

• Journal of Microbiology and Biotechnology
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• 제6권1호
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• pp.63-67
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• 1996

Bacteriocin-producing microorganism was isolated from Kimchi. The microorganism was identified as a Lactobacillus sp. and named Lactobacillus sp. JB-42. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For the maximum yield of bacteriocin from Lactobacillus sp. JB-42, the cell should be harvested at the early stationary phase and the temperature, pH and NaCl concentration should be $30^{\circ}C$, pH 7 and without the addition of NaCl, respectively. Sucrose, glucose or fructose should be used as a carbon source and organic nitrogen such as tryptone should be used as a nitrogen source for the best yield. The production of bacteriocin was related to the cell growth of Lactobacillus sp. JB-42 indicating the role of Lactobacillus in the Kimchi fermentation process.

• 아시안잔디학회지
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• 제6권1호
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• pp.1-10
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• 1992

This experiment was carried out to investigate the effect of mixing ratio of soil amending materials such as peat. perlite, active carbon, and zeolite on the growth and quality of creeping bentgrass (Agrostis pa1ustris var. Penncross). The results of this experiment were surnrnarized as follows; 1.Nitrogen content of clippings was increased by the treatment of peat and active carbon. hut the other phosphorus, potassium, calcium and magnesium content were increased in addition of,5% to 10% of peat, and 2.5% to 5% of active carbon. 10% of perlite. and 4% of zeolite 2.Fast growth and good quality of bentgrass were showed at the addition of 10% peat. l0% perlite.2.5~% active carbon, and 4% zeolite But over treatment of peat and active carbon induced inhi-bition of root growth and occurrence of disease.

• Journal of Microbiology
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• 제34권3호
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• pp.255-262
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• 1996

The lipopolysaccharide (LPS) yields were measured in Rhodobacter capsulatus under several conditions by the ELISA method. The purification of LPS was done by affinity chromatography of IgG coupled CNBr-activated sepharose-4B instead of ultra-centrifugation. The purity of the LPS didn't show much difference between affinity chromatography and ultra-centrifugation method, but affinity chromatography method required much fewer organisms and was more convenient. LPS yield was measured in ng units by the ELISA method. Mannitol was a better single carbon source than other sugars, but mixing two carbon sources resulted in greater LPS yields than any sugar alone. LPS yield was directly proportional to $NH_ 4CI$ concentration, with optimum yields at 0.05% nitrogen. In contrest to LPS yields, which decreased at 0.005% nitrogen concentration total protein was increased 16 times. Calcium influenced LPS yields. At 0.7 mM $CaCI_ 2$, the LPS yield was 16.5 $\mu$g/mg DW, five times the yield without calcium.

• Journal of Microbiology and Biotechnology
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• 제2권4호
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• pp.273-277
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• 1992

Treatment of poultry feces with Tolura sp. CH-30 produced a material that was significantly deodorized and showed a promotive effect on plant growth. Tolura sp. CH-30 grew on the poultry feces, deodorizing the feces by assimilation of volatile fatty acids, which are the source of the offensive odor, as a carbon source. Significant degradation of uric acid also occurred. In the treatment of feces with Tolura sp. CH-30, it was possible to deodorize feces in a short time, but reduction in the amount of urate-N was not enough. Urate-N inhibited plant growth due to an excessive nitrogen content produced as a result of rapid decomposition. Therefore, we propose a recycle-treatment plan using poultry feces treated with Tolura sp. CH-30. After the recycle-treatment, the amount of urate-N contained in the recycle-treated poultry feces was small and the recycle-treated poultry feces showed a promotive effect on plant growth when it was added at a nitrogen content of 1.6 g/600 g soil/pot.

• 한국미생물·생명공학회지
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• 제24권2호
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• pp.185-190
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• 1996

Some microorganisms including yeasts produce surface tension-decreasing biosurfactants. The strain G-1, the best producer of biosurfatants was isolated from the soil and identified as Rhodotorula sp., which was not discribed any report. The Rhodotorula sp. G-1 produced biosurfactant from vegetable oils, but failed to produce it from n-alkane or carbohydrate. Yeast extract was found to be more effective for the biosurfactant production as nitrogen source than any other inorganic nitrogen source. The composion of the optimal medium contained the following conponents: soybean oil 4%, glucose 2%, yeast extract 0.5%, KH$^{2}$PO$^{4}$ 0.1%, K$^{2}$HP0$^{4}$ 0.l%, MgSO$^{4}$ 5%, CaCl$^{2}$ 0.01%, NaCl 0.01%, pH 6.0. The surface tension activity was increased to 14% when, at first, the culture broth was fermented with only soybean oil as carbon sourse, and after 90 hours, feeded glucose, than that Of glucose and soybean oil added to it simultaneously. The maxium yield of the biosurfactant was about 15 g/l by after 90 hours, the feeding method of glucose.

• KSBB Journal
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• 제7권2호
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• pp.102-106
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• 1992

본 연구에서는 C. bombiocola에서 생성되는 미생물 계면활성제인 Sophorolipid의 생산관여인자 중, 질소원의 종류에 따른 영향과 C/N비율에 따른 생성량의 변화를 관찰하였다. 유기질소원이 비교적 놓은 생성량을 보였으며 그 중 pepton의 경우가 80g/L에 해당하는 높은 생성량을 보였다. C/N의 비율의 변화에 의한 Sophorolipid의 생성량의 변화 양상이 질소원의 종류에 따라 각기 다르게 나타났다. 또한 탄소원의 50%을 배양 이틀후 공급하는 방법으로 Sophorolipid가 약 30% 증가됨을 알 수 있다.

• Korean Chemical Engineering Research
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• 제47권6호
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• pp.788-794
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• 2009

황화수소 및 메틸머캡탄($CH_3SH$) 또는 DMS($CH_3SCH_3$)와 같은 황화합물들의 악취를 효율적으로 처리하기 위하여 축분으로 오염된 토양으로부터 sodium thiosulfate 또는 유리황과 같은 기질을 이용하여 미생물의 분리 및 동정을 하고 분리된 미생물들의 여러 가지 pH, 배양온도, 산소조건, 기질(황화합물) 농도, 질소원 및 탄소원의 농도 및 배양기 교반속도 등의 배양조건 하에서의 배양특성을 관찰하고 적정배양조건을 구축하였다. KD-1212와 DAH-1056 균주의 최적 pH는 각각 7.0 및 4.0이었으며 최적배양온도는 $30{\sim}35^{\circ}C$ 범위였다. 또한 독립영양미생물인 ED-1138 균주를 다른 오염된 토양에서 분리하였다. 균주를 고정한 바이오필터의 악취처리에 있어서 균주 DAH-1056이 기존 균주 Thiobacillus sp. IW보다 황화수소 제거능이 우수하였다. KD-1212 균주를 이용하여 황화합물의 농도 및 질소원 및 탄소원에 대한 성장특성을 조사한 결과, KD-1212는 sodium thiosulfate의 25 mM 농도에서 성장이 가장 잘되었고, 탄소원으로는 glucose와 maltose를 잘 이용하는 것으로 나타났다. 그리고 질소원으로는 yeast extract를 잘 이용하였으며 0.5% 농도에서 성장이 가장 잘 되었다.