Degradation of Indole by Acinetobacter sp. KL-9 with Production of Indigo

Acinetobacter sp. KL-9에의한 indole 분해 및 Indigo 생성의 특성

  • Kim, Jin-Wan (Department of Microbiology, Changwon National University) ;
  • Kim, Jin-Kyoo (Department of Microbiology, Changwon National University) ;
  • Lee, Soo-O (Department of Microbiology, Changwon National University) ;
  • Lee, Kyoung (Department of Microbiology, Changwon National University)
  • 김진완 (창원대학교 자연과학대학 미생물학과) ;
  • 김진규 (창원대학교 자연과학대학 미생물학과) ;
  • 이수오 (창원대학교 자연과학대학 미생물학과) ;
  • 이경 (창원대학교 자연과학대학 미생물학과)
  • Published : 2001.03.01

Abstract

Indole and its derivatives form a class of toxic recalcitrant environmental pollutants, Abacte-rium, strain KL-9 was isolated from soil with indole as a sole source of carbon and nitrogen. KL-9 was identified as Acinetobacter sp. on the basis of 16 S rRNA gene sequence, fatty acid and quinone compositions. This identification was also confirmed by the ability of carbon source utilization and other biochemical tests. The growth of Acinetobacter sp. KL-9 was fastest with 0.3mg/ml of indole as was inhibited by higher than 0.5mg/ml of indole in the medium, KL-9 with indole also produced indigo. The formation of indigo was stimulated inthe presence of glucose, which is not a growth-suppoting carbon source for KL-9. Additional biotransformation evidence showed that anthranilate is an intermediate for the degradation of indole KL-9.

Keywords

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