• Title/Summary/Keyword: Capsicum annum

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Plant Regeneration via Organogenesis from Seed Explants in Red Pepper (Capsicum annuum L.)

  • Lee, Kwang-Woong
    • Journal of Plant Biology
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    • v.39 no.3
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    • pp.167-172
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    • 1996
  • Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

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Antioxidant and Physiological Activities of Capsicum annuum Ethanol Extracts (고추 에탄올 추출물의 항산화 효과 및 생리활성에 관한 연구)

  • Kim, Hun-Joong;Hong, Chung-Oui;Nam, Mi-Hyun;Ha, Young-Min;Lee, Kwang-Won
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.6
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    • pp.727-732
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    • 2012
  • The goal of this study is to determine the activities of antioxidants and antiglycation from the extracts of various Capsicum annum (known as pepper) ethanolic extract (CAE). We tested the extracts of Capsicum annum seeds and pericarps using 70% ethanol. The CAE showed antioxidant activities in a 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay, 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging assay, ferric-reducing antioxidant power assay, total flavonoid content, and total polyphenol content. Also, the physiological activities of CAE on glycation inhibition activities, anti-${\alpha}$-glucosidase activities, and tyrosinase activities were measured. As a result, green and red Capsicum annuum seeds show higher levels of antioxidant activities. In addition, the physiological activities are also more effective in the seeds than in the plant pericarps. A radar chart proves that antioxidants and physiological activities are more effective coming from the seeds. And the red Capsicum annuum seeds are more effective than the green ones.

Effects of Number of Branches on the Growth and Yield of Sweet Pepper(Capsicum annuum L) in Long Term Bag-hydroponics (단고추의 장기 자루식 양액재배시 분지수가 생육 및 수량에 미치는 영향)

  • 김경제;라상욱;우인식;신동기;문창식;김진한
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1997.05a
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    • pp.36-39
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    • 1997
  • 단고추(Capsicum annum)는 중앙아메리카와 남아메리카의 열대지방이 원산지로 알려져 있고 매운맛이 없으며 미숙과를 주로 샐러드용으로 이용하는 원추형 과실을 착생하는 품종군으로 일반고추재배와는 달리 반드시 정지 및 유인을 해야만 좋은 과실을 생산할 수 있으며, 유인 및 관리정도에 따라 품질의 차이가 커 이것이 가격에 영향을 미쳐 농가의 소득과 직결되여 있다. 정지와 유인은 보통재배의 경우 U자형과 V자형으로 하고 있으나 노동력이 많이 소요된다. (중략)

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Enzymatic studies on capsaicin, the hot component of capsicum annum II A method of assaying capsaicin in kochuzang (고추의 신미성분Capsacin에 대한 효소화학적 연구 (제 2 보) 고추장중 신미성분의 정량법에 관하여)

  • 한구동;이상섭
    • YAKHAK HOEJI
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    • v.4 no.1
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    • pp.56-59
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    • 1959
  • We composed a method of assaying Capsaicin in Kochuzang, which is the most characteristic hot seasoning or food in Korean foods, by making use of Fujida's $method^{2}$). Capsaicin, a hot ingredient of hot pepper, was isolated from the acetone extract of dried Kochuzang with paperchromatography and anlayzed quantitatively with Electrophotometry.

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Enzymatic Studies on capsaicin, The hot component of capsicum annum III Studies on the Capsicin in Kochuzang (고추의 신미성분 Capsacin에 대한 효소화학적구 (제 3 보) 고추장중 Capsaicin 소장에 관하여)

  • 한구동;이상섭;최순진
    • YAKHAK HOEJI
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    • v.4 no.1
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    • pp.60-62
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    • 1959
  • We found a fact, when Kochuzang goes on ferment, that capsaicin in Kochuzang breaks down to other substances. And those substances are less hot than original capsaicin. The degradation of capsaicin depends on the concentration of table salt in Kochuzang. Nevertheless, these is rather no difference between a Kochuzang which contains no salt and the other one which contains sufficient salt when we assay them by Electrophotometry. We can explain this contradiction by making an assumption that the degradation might be happened at the acid radical part of capsaicin and not at Vanillyl radical.

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Isolation and culture of protoplasts from leaf tissue of Capsicum annnum var. accumnatum Fingerh and C. frutescens L. [Syn. C. minmum Roxb.] (Bird chilli)

  • Lee, Kue-Jae;Lee, Wang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.10b
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    • pp.20-20
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    • 2003
  • Isolation and culture of leaf protoplasts from two chilli cultivars (Capsicum annuum var. accumnatum and Bird chilli) were developed to enhance selection process in the somatic hybridization programmes. In order to isolate the protoplasts from leaves of these two chilli cultivars different incubation periods (3, 5 and 10 hours) were tested with combinations of enzyme mixtures containing cellulase and macerozyme. Leaves were incubated on three enzyme mixtures (2% cellulase +0.4% macerozyme, 1% cellulase +0.2% macerozyme and 0.5% cellulase +0.1% macerozyme in 13% mannitol) at 251oC in the dark. Three hours of incubation using 2% cellulase and 0.4% macerozyme was the best for the protoplast isolation of both chilli cultivars tested. The yield was 5${\times}$108protoplasts/m1/g leaf tissue in both chilli varieties. It was found that in the mixed nurse method using Nagata and Takebe (NT) medium supplemented with 1.0mg/12,4-D, NAA and BAP with 0.5M mannitol and 1.2% Sea Plaque agarose is the best medium for protoplast culture. Protoplasts of Capsicum annum var. accumnatum were alive for 14 days forming cell walls and initiating cell division.

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Isolation and culture of protoplasts from leaf tissue of Capsicum annnum var. accumnatum Fingerh and C. frutescensL. [Syn. C. minimum Roxb.] (Bird chilli)

  • Lee, Kue-Jae;Lee, Wang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.10a
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    • pp.50-58
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    • 2003
  • Isolation and culture of leaf protoplasts from two chilli cultivars (Capsicum annuum var. accumnatum and Bird chilli) were developed to enhance selection process in the somatic hybridization programmes. In order to isolate the protoplasts from leaves of these two chilli cultivars different incubation periods (3, 5 and 10 hours) were tested with combinations of enzyme mixtures containing cellulase and macerozyme. Leaves were incubated on three enzyme mixtures (2% cellulase + 0.4% macerozyme, 1% cellulase + 0.2% macerozyme and 0.5% cellulase + 0.1 % macerozyme in 13% mannitol) at 251oC in the dark. Three hours of incubation using 2% cellulase and 0.4% macerozyme was the best for the protoplast isolation of both chilli cultivars tested. The yield was 5 ${\times}$ 108protoplasts/ml/ g leaf tissue in both chilli varieties. It was found that in the mixed nurse method using Nagata and Takebe (NT) medium supplemented with 1.0mg/12,4-D, NAA and BAP with 0.5M mannitol and 1.2% Sea Plaque agarose is the best medium for protoplast culture. Protoplasts of Capsicum annum var. accumnatum were alive for 14 days forming cell walls and initiating cell division.

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Plant growth promoting effect of 4-quinolinone metabolites from Pseudomonas cepacia and 4-quinolinone-3-carboxylate derivatives on red pepper plant (Capsicum annum) (Pseudomonas cepacia로부터 유래한 4-quinolinone 대사물질과 4-quinolinone-3-carboxylate 유도체의 고추(Capsicum annum)에서의 생장촉진 효과)

  • Moon, Surk-Sik;Myung, Eul-Jae;Cho, Soon-Chang;Park, Jae-Bum;Chung, Bong-Jin
    • The Korean Journal of Pesticide Science
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    • v.6 no.2
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    • pp.64-71
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    • 2002
  • Plant growth promoting activity of quinolinone metabolites, 2-(2-hepteny)-3-methyl-4-quinolinone (1), 2-heptyl-3-methyl-4-quinolinone, and 2-nonyl-3-methyl-4-quinolinone, produced by Pseudomonas cepacia and ethyl 2-methyl-3-alkyl-4-quinolinone carboxylates chemically synthesized were tested by using seed-germination assay, growth increments in plant height after foliar applications. Plant height increment, fresh weight, and the number of fruits were measured after seed-soaking and drench treatment. Compound 1 among the natural products showed a consistent growth promoting effect in seed-germination and plant height after a foliar application. After a seed-soaking and drench treatment, compound 1 and synthetic ethyl 2-methyl-4-quinolinone-3-carboxylate (5) showed a significant enhancement in fresh weight and the number of fruits after harvest. Compound 1 and 5 increased the number of fruits per plant by 44% and 84% over the control, respectively.

The Allelopathic Effects of Lantana camara on Seed Germination and Growth of Selected Bioassay Species

  • Senarathne, S.H.S.;Fernando, R.D.V.;Sangakkara, U.R.
    • Korean Journal of Weed Science
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    • v.31 no.3
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    • pp.271-278
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    • 2011
  • The allelopathic effects of Lantana camara L. (Family:Verbenaceae) on germination and seedling establishment of some agricultural crops and weed species have been identified. Aqueous extracts of dry leaves and contaminated soil where L. camara is grown were used to verify allelopathic effect on seed germination of five bioassay species; Raphanus sativas, Capsicum annum, Lycopersicum esculantem, Crotalaria juncia and Chromoleana odorata. Fifty seeds from each bioassay species were placed in a petri dish containing leaf extracts or contaminated soil, and seed germination were examined after 3 days. The plant house experiments were carried out to evaluate the impact of L. camara contaminated soil and leaf debris using L. esculantem as the indicator plant. Seed germination of L. esculentem, C. junica and Capsicum annum was significantly inhibited by L. camara contaminated soil. However, the degree of inhibition varied among the bioassay species. The aqueous extract of dry leaves of L. camara was highly phytotoxic and it significantly reduced seed germination of all bioassay species. There was a decline in plant height, leaf area and shoot dry weight of tomato only in early growth stages when grown in L. camara contaminated soils. However, incorporation of leaf debris into soil affected the vegetative growth of tomato in early stages when the leaf debris concentration was increased. Growth recovered at the latter part of the life cycle. On the basis of these results it can be concluded that the allelochemicals in L. camara contaminated soils are harmful to the seed germination of crop species. The adverse effect was present only during the early growth stages and it did not suppress the latter part of the plant growth. These responses are attributed to allelopathic effects which need confirmation under field conditions.