• Title/Summary/Keyword: Capillary method

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The Effect of Total Radiation Dose on Normal Spinal Cord of Hybrid Mice -Early Pathological Changes- (총 방사선 조사량이 잡종 백색 마우스의 정상 척수에 미치는 영향)

  • Shon, Sung-Won;Kim, Myung-Se;Choi, Won-Hee
    • Radiation Oncology Journal
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    • v.4 no.2
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    • pp.107-113
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    • 1986
  • Radiation myelitis is a rather rare, but irreversible fatal complication. Etiology, pathologic change, clinical symptoms and the method of diagnosis have been studied with animal experiments and human necropsies. In spite of massive studies, pathogenesis of post-irradiation myelitis and the level of tolerance dose still remain controversial. Thoracolumbar spine of 110 hybrid mice were irradiated with orthovoltage x-ray machine. Mild capillary congestion and axonal swelling were observed in 1,000 rad irradiated specimens. Focal necrosis in 3,500 rad specimens, fragmentation of neural tissue in 4,000 rad specimens were also observed. These results suggest that 5,000 rad is not a completely safe tolerable dose which have been accepted and we cannot exclude direct radiation damage to news tissue as the causative pathology of radiation myelitis in addition to blood vessel damage.

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Effect of Ambient Temperature on Bovine Erythrocyte Sedimentation Rate as Measured by Angled Capillary Method (경사모세관법에 의한 우의 적혈구침강 속도에 미치는 환경온도의 영향)

  • Kim Kyeong-Jin;Lee Bang-Whan
    • Journal of Veterinary Clinics
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    • v.4 no.1
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    • pp.395-401
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    • 1987
  • In this study, the effect of ambient temperature on the 45$^{\circ}$micro ESR/hr of cattle blood were observed, and a correction chart for correcting observed values at any ambient temperature to standard values at 20$^{\circ}C$ was plotted. Besides, the effect of storage temperature of blood on the 45$^{\circ}$micro ESR/hr was surveyed. The results were as follows: 1. The values of the 45$^{\circ}$micro ESR/hr were increased as the ambient temperature were elevated(P<0.01), and lower the value of PCV, higher the effect of temperature on the 45$^{\circ}$micro ESR/hr was observed(P<0.01). 2. Regression of values of 45$^{\circ}$micro ESR to ambient temperature in all the group of different level of blood PCV showed linear regression with the highly significant coefficient of correlation. With the results, correction chart was drawn as in Fig. 1. 3. In the purpose to verify the reliability of correction chart, observed values of 45$^{\circ}$micro ESR/hr in field(out door) were corrected to values at 20$^{\circ}C$ by the correction chart(Fig. 1), comparing with the observed values at 20$^{\circ}C$ of standard temperature. No significant differences were found between two groups mentioned above. 4. In the study on the effect of storage temperature of the blood on the 45$^{\circ}$micro ESR/hr, group of storage temperature at 5$^{\circ}C$ showed statistically no significant differences untill 24 hours in contrast with standard control group.

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Genetic Variation Analysis of Arabidopsis (Arabidopsis thaliana L.) Plants Induced by Acute and Chronic Gamma Irradiation (감마선 완·급조사에 따른 애기장대의 유전적 유연관계 분석)

  • Goh, Eun Jeong;Kim, Jin-Baek;Ha, Bo-Keun;Kim, Sang Hoon;Kang, Si-Yong;Kim, Dong Sub
    • Journal of Radiation Industry
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    • v.5 no.4
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    • pp.347-352
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    • 2011
  • In order to identify the genetic relationship analysis by acute and chronic gamma irradiation, Arabidopsis (Arabidopsis thaliana L.) were irradiated with 200 Gy of gamma-rays using gamma-irradiator (3,000 Ci; Nordion, Canada) and gamma-phytotron (400 Ci; Nordion, Canada) for acute and chronic irradiation, respectively. Genetic relationship among two acute gamma-irradiated plants (A1 and A24) and three chronic gamma-irradiated plants (C1W, C2W, C3W) were analyzed using the amplified fragment length polymorphism (AFLP) technique compared with each non-irradiated plant. A total of 28 EcoRI and MseI primer combinations were used to screen 8 treatments by the ABI3130 capillary electrophoresis system. Amplified products by 28 primer sets showed 1,679 bands with an average of 51 bands per primer combination. Out of the total bands scored, 1,164 fragments were polymorphic bands, with different alleles existing among the treatments. The cluster analysis was performed using the UPGMA (Unweighted Pair Group Method using Arithmetic) in the computer program NTSYS-pc. In clustery analysis, acute gamma-irradiation showed higher genetic variation compared with chronic gamma-irradiation.

Quantification and Varietal Variation of Fatty Acids in Mulberry Fruits (뽕나무 계통별 오디종자의 지방산 조성 및 함량)

  • 김현복;김선림;성규병;남학우;장승종;문재유
    • Journal of Sericultural and Entomological Science
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    • v.45 no.2
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    • pp.75-79
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    • 2003
  • Fatty acids analysis was carried out with mulberry seed. As a result, palmitic acid, stearic acid, oleic cid and linoleic acid were detected as a major fatty acid. Their rates of composition were 8.8${\pm}$3.3, 4.2${\pm}$1.6, 5.8${\pm}$2.3 and 81.2${\pm}$6.9(%), respectively. The unsaturated fatty acids such as oleic acid and linoleic acid were abundant in mulberry seed. Paticularly, five accessions including ‘Gukkwang’, ‘Jaelaenosang’, ‘Sangbansibmunja (Jeonnam) ’, ‘Neunggeum’ and ‘Suwonnosang’ were observed only with linoleic acid. Unsaturated fatty acids are known to have physiological effects decreasing the serum cholesterol. From the above result, we confirm that mulberry fruits have abundant unsaturated fatty acids and anthocyanin pigments, and they are of great value as functional food resources.

Development of a New Type of Recombinant Hyaluronidase Using a Hexahistidine; Possibilities and Challenges in Commercialization

  • Park, Chaeri;Song, Yun-Kyoung;Kim, Young-Hyun;Jung, Yena;Park, Young-Ho;Song, Bong-Seok;Eom, Taekil;Kim, Ju-Sung;Kim, Sang-Hyun;Kim, Ji-Su;Kim, Sun-Uk;Lee, Sang-Rae;Kim, Ekyune
    • Journal of Microbiology and Biotechnology
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    • v.29 no.8
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    • pp.1310-1315
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    • 2019
  • Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and assess its function. Therefore, in order to find the most efficient active hyaluronidase, we produced several shortened hyaluronidases with partial removal of the N- or C-terminal regions. Moreover, we created an enzyme that connected six histidines onto the end of the hyaluronidase C-terminus. This simplified subsequent purification using $Ni^{2+}$ affinity chromatography, making it feasible to industrialize this highly active recombinant hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme. Therefore, this simple and effective isolation method could increase the availability of recombinant hyaluronidase for research and clinical purposes.

Outpatient Percutaneous Flexor Tenotomy for Diabetic Claw Toe Deformity with Ulcer (궤양을 동반한 당뇨성 갈퀴 족지에 대하여 외래에서 시행한 경피적 굴곡건 절단술)

  • Lee, Dong-Hun;Chung, Jin-Wha
    • Journal of Korean Foot and Ankle Society
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    • v.22 no.4
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    • pp.151-155
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    • 2018
  • Purpose: Toe ulcers have been implicated as a causative factor in diabetic foot amputation. The aim of this study was to evaluate the outcomes of percutaneous needle flexor tenotomies of diabetic claw toes with ulcers or pending ulcers. Materials and Methods: The authors undertook a retrospective chart review between January 2014 and June 2016 to identify those patients who underwent a percutaneous needle flexor tenotomy for diabetic claw toe deformities. We evaluated 54 toes in 42 patients. Twenty-four patients were female and the mean age at the time of operation was 57 years. The mean follow-up time was 11 months. Thirty-four patients (46 toes) had tip toe ulcers or pending ulcers and 8 patients (8 toes) had dorsal pending ulcers. All patients had palpable pulses and good capillary refill. Results: Forty-three of 46 tip toe ulcers (93.5%) healed without significant complications and 8 dorsal ulcers showed no specific changes within 5 weeks. There were no recurrent ulcers at final follow-up. Four patients developed transfer lesion of the adjacent toe and needed subsequent tenotomy. Conclusion: Percutaneous needle tenotomy in an outpatient clinic was an effective and safe method for treating toe ulcers in neuropathic patients to offload the tip of the toe so that ulcer healing could occur.

Enzyme-linked Immunosorbent Assay Strip Sensor for Rapid Detection of Staphylococcus aureus (Staphylococcus aureus 신속 검출을 위한 효소면역측정 스트립 센서)

  • Park, So Jung;Kim, Young-Kee
    • Applied Chemistry for Engineering
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    • v.22 no.5
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    • pp.522-525
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    • 2011
  • In this study, an established enzyme-linked immunosorbent assay and immuno-chromatography technique are combined to fabricate an immuno-strip sensor for the detection of S. aureus. The immuno-strip is manufactured by using four different functional membranes. The capture antibody is immobilized on the nitrocellulose membrane due to the high affinity and the capillary action through porous membranes induces a flow of sample. A colorimetric signal is appeared according to the enzyme reaction and is analyzed by the digital camera (qualitative analysis) and home-made image analysis software (quantitative analysis). Under the optimal conditions, samples with S. aureus in the range of $2.7{\times}10^4{\sim}2.7{\times}10^7CFU/mL$ can be detected by the colorimetric method within 30 min.

A study on possibility of using CST as a fouling evaluation index at MBR (MBR에서 fouling 평가지표로서 CST의 활용 가능성에 대한 연구)

  • Kim, Yun-Ji;Choi, Yun-Jeong;Sim, Tae-Suk;Hwang, Sun-Jin
    • Journal of Korean Society of Water and Wastewater
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    • v.35 no.6
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    • pp.379-387
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    • 2021
  • In general, trans-membrane pressure (TMP), flux, filtration resistance are used as indicators to evaluate the degree of fouling in MBR. However, they have limitations in determining the level of EPS generation, which is known as an important factor of fouling. Therefore, a new evaluation method is required to monitor the amount of EPS generation. In this study, the applicability of capillary suction time (CST), which is used to measure the dewaterability of sludge, was evaluated as an indirect fouling evaluation index. Statistical analysis was performed to evaluate the effect of EPS on CST, and to determine whether EPS has high similarity with representative fouling evaluation indicators and CST, and quantitatively compared them. As a result, the correlation coefficient between CST and bEPS was 0.7988, which was higher than the correlation coefficient between filtration resistance and bEPS. Since bEPS is a major factor inducing fouling by affecting the formation of the cake layer, it was evaluated that CST, which has a high correlation with bEPS, is suitable to represent EPS. In addition, it was evaluated that the correlation coefficient between filtration resistance and CST was high as 0.7187, which could be used as a fouling evaluation index.

Treatment of Facial Arteriovenous Malformations by Embolization: A Case Report (색전술에 의한 안면부 동정맥 기형의 치료 : 증례 보고)

  • Jeongyeon, Kim;Hyunsoo, Lim;Okhyung, Nam;Hyo-seol, Lee;Sungchul, Choi;Misun, Kim
    • Journal of the korean academy of Pediatric Dentistry
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    • v.49 no.2
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    • pp.228-233
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    • 2022
  • Arteriovenous malformations (AVMs) are rare congenital anomalies characterized by direct communication between arteries and veins that bypass the capillary bed. AVMs may not manifest clinically until late infancy or childhood. In particular, facial AVMs can cause urgent life-threatening dental events. A 5-year-old girl without a medical history visited the hospital because of spontaneous gingival bleeding around the posterior gingival area of the lower left 2nd primary molar. Angiography through the femoral approach under general anesthesia was performed for differential diagnosis and therapeutic option. The blood flow was effectively reduced after arterial embolization alone, and there was no evidence of recurrence at the 5-month follow-up. The present study reports that embolization of the affected vessels can be a more effective and safe method than surgical resection for the treatment of AVM during the growth period.

Research on the Manufacturing Technology for a PDMS Structure-Based Transpiration Generator Using Biomimetic Capillary Phenomenon (생체모방 모세관 현상을 이용한 PDMS 구조체 기반 증산발전기 제조기술 연구)

  • Seung-Hwan Lee;Jeungjai Yun;So Hyun Baek;Yongbum Kwon;Yoseb Song;Bum Sung Kim;Yong-Ho Choa;Da-Woon Jeong
    • Journal of Powder Materials
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    • v.30 no.3
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    • pp.268-275
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    • 2023
  • The demand for energy is steadily rising because of rapid population growth and improvements in living standards. Consequently, extensive research is being conducted worldwide to enhance the energy supply. Transpiration power generation technology utilizes the vast availability of water, which encompasses more than 70% of the Earth's surface, offering the unique advantage of minimal temporal and spatial constraints over other forms of power generation. Various principles are involved in water-based energy harvesting. In this study, we focused on explaining the generation of energy through the streaming potential within the generator component. The generator was fabricated using sugar cubes, PDMS, carbon black, CTAB, and DI water. In addition, a straightforward and rapid manufacturing method for the generator was proposed. The PDMS generator developed in this study exhibits high performance with a voltage of 29.6 mV and a current of 8.29 µA and can generate power for over 40h. This study contributes to the future development of generators that can achieve high performance and long-term power generation.