• 생태와환경
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• 제54권3호
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• pp.247-256
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• 2021

동물플랑크톤이 식물플랑크톤을 선택적으로 섭식하는 특성에 대한 이해는 수생태계 먹이사슬 내의 물질 이동에 중요하다. 하지만 해부를 통한 위내용물 추출 방법은 소형 요각류를 대상으로 적용하기에는 적절하지 않고, 유전자가 유실되거나 위내용물이 아닌 개체 외부의 유전자로 인해 오염될 가능성이 존재한다. 본 연구에서 호 내 식물 플랑크톤 조성 및 기타 환경이 상이한 두 지점을 선정하여 모든 지점에서 지속적으로 출현하는 기수성 요각류인 Sinocalanus tenellus를 대상으로 위내용물의 유전자 분석을 수행하였다. 요각류 개체 외부의 DNA를 제거하는 데 2.5%로 희석한 시판용 표백제(차아염소산나트륨 5.4%)에 2분간 처리하여 증류수로 2회 세척한 뒤 유전자를 추출하였다. 추출된 유전자는 23S rRNA을 증폭하여 서열분석을 실시하였다. Capillary sequencing 분석 결과, 원수와 처리수 및 요각류 위내용물에서 다양한 분류군(규조강, 녹조강, 남조강, 와편모조강, 은편모조강, 황갈조강)에 속하는 식물플랑크톤이 검출되었으며, 새만금호 내 시공간 차이에 따라 상이한 경향을 보였다. 현미경을 이용하여 동정한 식물플랑크톤 군집 조성의 경우 규조강이 우점한 반면, 동일한 원수의 유전자 분석(capillary sequencing) 결과에서는 주로 녹조강, 남조강 및 와편모조강이 우점하여 다소 상반된 경향을 나타냈다. 본 연구에서 적용한 위내용물 분석에 특화된 외부 유전자 제거 전처리 방법은 농도와 처리시간 조절 등의 응용방법에 따라 다양한 동물플랑크톤 분류군에 적용이 가능할 것으로 사료된다.

• Journal of the Optical Society of Korea
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• 제12권1호
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• pp.19-24
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• 2008

In this paper, we describe the fabrication process and the characteristics of polarization maintaining photonic crystal fibers (PM-PCFs). The PM-PCF is fabricated by stack-and-draw method, i.e., stacking silica capillary tubes (making a PM-PCF preform) and drawing to optical fiber. Firstly, a PM-PCF preform is formed by stacking two kinds of capillary tubes around a solid silica rod and jacketing these stacked tubes with an outer silica tube (out-jacket tube). Later, the desired preform is drawn to a fiber in a high temperature drawing tower. We also compare the polarization properties such as polarization dependent loss, birefringence, and differential group delay of the fabricated PM-PCF with those of the conventional PANDA PM fiber.

• 대한화학회지
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• 제31권3호
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• pp.244-249
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• 1987

미생물의 대사산물 중에서 각각의 균주에 특징있게 생성되는 탄소수 $C_2-C_5$인 휘발성 유리산의 정성 정량 분석자료는 혐기성 미생물을 확인하는 정보를 준다. 수용성 매체에 용해되어 있는 미생물의 대사산물을 처리함에 있어서 흡착제로 Chromosorbp를 사용한 liquid-solid extraction 방법에 대하여 조사 검토하였다. Chromosorb P는 친수성인 흡착제이므로 그 표면에 간섭물질인 수용성 성분들이 흡착되어 남아있는 반면에 비이온화성인 휘발성 유리산들은 ether에 의해서 정량적으로 회수가 된다. 이와 같이 신속하며 효율적으로 처리된 시료를 5% Carbowax 20M/0.75% $H_3PO_4$로 coating 된 stainless steel capillary column 으로 정성 정량분석하였다.

• 성인간호학회지
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• 제16권2호
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• pp.222-232
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• 2004

Purpose: The purpose of this study was to identify the change in foot care knowledge, self care behavior, and physiologic indexes after foot reflexomassage education program, and the related factors. Method: Data were collected from 20 patients who were visited out patients clinic. The change and difference were analyzed with non-parametric statistics. Result: There were significant differences in foot care knowledge(P=.001), self care behavior(P=.000), dosalis pedis blood flow volume (P=.011), skin temperature of foot(P=.001), dorsalis pedis pulse(P=.000), capillary filling time of foot (P=.000) between pre and post. The level of changes of foot care knowledge was significant differences according to admission experience (P=.049), and negative related to systolic blood pressure(P=.028). The level of changes of self care behavior was related to age(P=.049), that of dosalis pedis blood flow was significant difference according to smoking(P=.042), that of skin temperatureof foot(P=.002) and dosalis pedis pulse(P=.038) were significant difference according to weight. The level of changes of capillary filling time of foot was related to diagnosis period(P=.014). Conclusion: Foot-Reflexo-Massage education program is an effective nursing intervention to promote foot care in diabetic patients. And the related factors can be recommended for the management of diabetic patients.

• 한국정밀공학회지
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• 제23권9호
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• pp.164-173
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• 2006

The cooling capacity of a micro-heat pipe is mainly governed by the magnitude of capillary pressure induced in the wick structure. For microchannel wicks, a higher capillary pressure is achievable for narrower and deeper channels. In this study, a metallic micro-heat pipe adopting high-aspect-ratio microchannel wicks is fabricated. Micromachining of high-aspect-ratio microchannels is done using the laser-induced wet etching technique in which a focused laser beam irradiates the workpiece placed in a liquid etchant along a desired channel pattern. Because of the direct writing characteristic of the laser-induced wet etching method, no mask is necessary and the fabrication procedure is relatively simple. Deep microchannels of an aspect ratio close to 10 can be readily fabricated with little heat damage of the workpiece. The laser-induced wet etching process for the fabrication of high-aspect-ratio microchannels in 0.5mm thick stainless steel foil is presented in detail. The shape and size variations of microchannels with respect to the process variables, such as laser power, scanning speed, number of scans, and etchant concentration are closely examined. Also, the fabrication of a flat micro-heat pipe based on the high-aspect-ratio microchannels is demonstrated.

• Transactions on Electrical and Electronic Materials
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• 제12권3호
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• pp.119-122
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• 2011

A series of mesoporous silicalites was synthesized using different compositions of tetraethylorthosilicate and methyltriethoxysilane (MTES) as the silica source. Cetyltrimethylammonium bromide was used as the organic template. Their detailed pore structures were investigated by transmission electron microscopy, X-ray diffraction, and N2 adsorption method. The thermal properties of these silicalites were studied by thermogravimetric analysis. The increased amount of MTES destroyed mesoporous channels and reduced pore sizes from 3.4 nm to 2.8 nm in calcined silicalites. The calcined silicalite transformed completely into an amorphous state at 30% MTES loading. Methyl pending groups of MTES hindered the structural ordering of ≡Si-O- frameworks, resulting in an amorphous structure. This was caused by the insufficient formation of supramolecular assembly with the organic template. No capillary condensation step was found in MS 7/3 silicalite. The other capillary condensation steps shifted toward the lower relative pressure with increasing MTES content, indicating the reduction of pore sizes.

• Applied Microscopy
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• 제27권4호
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• pp.391-402
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• 1997

본 연구는 고단백뇨를 보이는 신장질환모델마우스(FGS/Kist mouse)의 사구체에 대한 미세구조학적 변화를 관찰하고자 수행하였다. 전반적으로 사구체내의 모세혈관은 주변부의 혈관사이세포(mesangial cell) 부위의 확장으로 인하여 관강이 좁아져 있었으며 관강내에는 점액양 물질들이 흔히 관찰되었다. 또한 사구체바닥판(basal lamina)이 모세혈관 사이의 공간쪽으로 돌출되어져 있는 소견들도 볼 수 있었다. 혈관사이세포 부위에는 주변부와 뚜렷이 구분되는 전자밀도가 높은 물질들이 많이 차여져 있었다. 한편, 사구체바닥판에 인접하여 존재하고 있는 발세포들(podocytes)은 세포질내 많은 공포들이 관찰되었고, 발세포들이 내고 있는 발세포발(foot process)들은 서로 융합되거나 소실되어 매우 불규칙적인 소견을 보였다.

• Fibers and Polymers
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• 제4권3호
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• pp.135-144
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• 2003

Permeability of the preform is one of key factors in design of RTM (Resin Transfer Molding) mold, determination of processing conditions, and modeling of flow in the mold. According to previous studies, permeability measured in the unsaturated fiber mats are higher than that in the saturated fiber mats by about 20% because of the capillary pressure. In this study, permeabilities of several fiber preforms are measured for both saturated and unsaturated flows. A saturated experiment of radial flow has been adopted to measure the permeability of anisotropic fiber preforms with high fiber content, i.e., circular braided preforms. In this method, four pressure transducers are used to measure the pressure distribution. Permeabilities in different directions are determined and the experimental results show a good agreement with the theory. Since permeability is affected by the capillary effect, permeability should be measured in the unsaturated condition for the textile composites to be manufactured under lower pressure as in the Vacuum Assisted Resin Transfer Molding (VARTM).

• 한국식품과학회지
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• 제23권2호
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• pp.217-223
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• 1991

Gel식품의 구조적 특성을 밝히는 일은 특수한 장치나 분석방법이 요구되어 왔으나 본 연구에서는 조작이 간편하고 확산식에 잘 맞는 농도의 해를 구할 수 있는 capillary method를 통해 확산량과 확산계수를 예측하였다. 확산량은 거리, 시간, agar% 농도를 독립변수로 하여 도시하고 추정된 몇 가지 함수식으로부터 SPSS 통계 package 중 regression program을 이용해 단순회귀시켜 R-square가 가장 높은 식을 채택하고 다시 각 독립변수에 대한 함수식을 이용해 다중회귀분석을 실시함으로써 최종 model식을 수립하였다. 기존의 확산계수 예측방법은 Fick의 제 2법칙의 해식으로부터 예측되는 것이 일반적이었으나 그 식이 난해하고 시간이 많이 걸려 본 연구에서는 좀 더 간편한 방법으로 확산계수를 구하고자 수치해석적 방법에 따라 확산계수를 예측하였으며 그 산술평균을 확산계수 D값으로 나타내었다.

• 한국재료학회지
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• 제21권10호
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• pp.525-531
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• 2011

Two dimensional (2D) arrays of noble metal nanoparticles are widely used in the sensing of nanoscale biological and chemical events. Research in this area has sparked considerable interest in many fields owing to the novel optical properties, e.g., the localized surface plasmon resonance, of these metallic nanoarrays. In this paper, we report successes in fabricating 2D arrays of gold nano-islands using nanosphere lithography. The reproducibility and the effectiveness of the nano-patterning method are tested by means of spin coating and capillary force deposition. We found that the capillary force deposition method was more effective for nanospheres with diameters greater than 600 nm, whereas the spin coating method works better for nanospheres with diameters less than 600 nm. The optimal deposition parameters for both methods were reported, showing about 80% reproducibility. In addition, we characterize gold nano-island arrays both geometrically with AFM as well as optically with UV-VIS spectrometry. The AFM images revealed that the obtained nano-arrays formed a hexagonal pattern of truncated tetrahedron nano-islands. The experimental and theoretical values of the geometric parameters were compared. The 2D gold nano-arrays showed strong LSPR in the absorption spectra. As the nano-islands increased in size, the LSPR absorption bands became red-shifted. Linear dependence of the plasmon absorption maximum on the size of the gold nano-islands was identified through the increment in the plasmon absorption maximum rate for a one nanometer increase in the characteristic length of the nano-islands. We found that the 2D gold nano-arrays showed nearly seven-fold higher sensitivity of the absorption spectrum to the size of the nano-islands as compared to colloidal gold nano-particles.