• 대한한방종양학회지
• /
• 제7권1호
• /
• pp.61-75
• /
• 2001

The purpose of this study is to prove the angiogenesis effects of WhaYoungJiTongTang (hereinafter referred to as the 'WYJTT'). For the study, by utilizing liver cancer cell line; SK-HEP-1, lung cancer cell line: A549, stomach cancer cell line: AGS and bovine capillary endothelial cell: BCE, the effects of the WYJTT on toxicity and proliferation ability of cells and the effects on anti-angiogenesis of bovine capillary endothelial cell and of mice's aorta were studied. 1. Cell viability assay In comparison with the control group, when $100{\mu}g/ml$ of WYJTT was injected, the viability was reduced in SK-Hep-1, $400{\mu}g/ml$ in A549 and $200{\mu}g/ml$ in AGS. 2. Cell proliferation assay In comparison with the control group, when $600{\mu}g/ml$ of WYITT was injected, DNA synthesis was reduced to 35.1% in the SK-Hep-1, 56.0% in A549, and 25.8% in BCE (bovine capillary endothelial cell); and when $400{\mu}g/ml$ was injected, DNA synthesis was reduced to 12.1 in AGS. 3. Tube formation assay In the event that BCE is injected with WYJTT in each of its content gradient, the anti-angiogenesis was effective in amounts of $400{\mu}g/ml$ with 6 hours of the treatment. 4. Aortic ring assay In comparison with the control group, the angiogenesis was restricted to the remarkable degree in amount of $200{\mu}g/ml$: 10% in $400{\mu}g/ml$; and fully inhibited in each of $800{\mu}g/ml$ and $1600{\mu}g/ml$. As a result of the experiments mentioned above, WYJTT showed its anti-angiogenesis effects against cancer cell line.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2004년도 Asia Display / IMID 04
• /
• pp.727-730
• /
• 2004

Closed-cell type barrier ribs such as honeycomb, SDR, inverse SDR and waffle types were produced using capillary molding process. Phosphor layers were formed by osmosis coating process on those barrier ribs. Using the rear plate with closed-cell ribs, the luminance and its efficiency was measured. The results demonstrated a significant improvement in efficiency by combining closed-cell type ribs with a new phosphor forming technology

• 한국태양에너지학회 논문집
• /
• 제32권5호
• /
• pp.118-123
• /
• 2012

Water balance has a significant impact on the overall fuel cell performance. Maintenance of proper water management should provide an adequate membrane hydration and avoidance of water flooding in the catalyst layer and gas diffusion layer. Considering the important of advanced water management in PEM fuel cell, this study proposes a simple one dimensional water transportation model of PEM fuel cell for use in a dynamic condition. The model has been created by assumption that the output is the water liquid saturation difference. The liquid saturation change is the total difference between the additional water and the removal water on the system. The water addition is obtained from fuel cell reaction and the electro osmotic drag. The water removal is obtained from capillary transport and evaporation process. The result shows that the capillary water transport of low temperature fuel cell is high because the evaporation rate is low.

• 대한기계학회논문집B
• /
• 제35권5호
• /
• pp.487-490
• /
• 2011

본 논문은 동결과 융해가 고분자 전해질 연료전지 내의 기체확산층에 미치는 영향에 대해 X-선 단층 촬영법을 이용하여 수행한 실험적 연구이다. 고분자 전해질 연료전지는 외부 온도가 0 도 이하가 되면 내부의 물이 동결되며, 외부 온도가 0 도 이상으로 상승하면 다시 녹는 과정을 겪게 된다. 이 과정은 연료전지의 내부 구조에 변형을 야기하고 이로 인해 연료전지의 전력과 수명의 감소를 야기하게 된다. 기체확산층은 연료전지 내부에서 가장 두꺼운 다공성층이며, 이로 인해 가장 많은 변형이 발생된다. 본 연구에서는 포항 방사광 가속기의 X-선 단층 촬영법을 이용하여 물의 동결과 융해 과정이 기체확산층의 내부 구조에 미치는 변화를 관찰하였다. 이 기체확산층의 구조 변화가 고분자 전해질 연료전지의 전력 생산과 수명에 미치는 영향에 관해 논의하였다.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2004년도 Asia Display / IMID 04
• /
• pp.571-574
• /
• 2004

Symmetric closed-cell type barrier ribs of PDP were formed by capillary molding process using molds prepared by inclined UV lithography process. The effects of inclining angle of barrier ribs on the sintering shrinkage and luminance of panel were examined. The results indicate that the barrier ribs of inclined morphology affect the sintering shrinkage and luminance efficiency significantly.

• Korea-Australia Rheology Journal
• /
• 제16권3호
• /
• pp.153-160
• /
• 2004

Open microcellular foams having small-sized cell and good mechanical properties are desirable for many practical applications. As an effort to reduce the cell size, the microcellular foams combining viscosity improvers into the conventional formulation of styrene and water system were prepared via high internal phase emulsion polymerization. Since the material properties of foam are closely related to the solution properties of emulsion state before polymerization, the flow behavior of emulsions was investigated using a controlled stress rheometer. The yield stress and the storage modulus increased as viscosity improver concentration and agitation speed increased, due to the reduced cell size reflecting both a competition between the continuous phase viscosity and the viscosity ratio and an increase of shear force. Appreciable tendency was found between the rheological data of emulsions and the cell sizes of polymerized foams. Cell size reduction with the concentration of viscosity improver could be explained by the relation between capillary number and viscosity ratio. A correlative study for the cell size reduction with agitation speed was also attempted and the result was in a good accordance with the hydrodynamic theory.

• 한국수의병리학회지
• /
• 제5권2호
• /
• pp.79-80
• /
• 2001

A gingival mass was detected from a 1-year-old female Great Dane dog. After surgical removal, the lesions recurred in 2 weeks and died of septicemia. Characteristic histologic features were large numbers of multinucleated giant cells which were connected with capillary vessels. Neovascularization was prominent with mononuclear and polynuclear cell infiltration. Overall features of these lesions except for giant cell infiltration were similar to granuloma. From these results, a gingival mass excised from a dog was diagnosed to be a peripheral giant cell granuloma (PGCG). This is the first report of canine subcutaneous PGCG in Korea.

• 한국방사성폐기물학회:학술대회논문집
• /
• 한국방사성폐기물학회 2015년도 추계학술논문요약집
• /
• pp.171-172
• /
• 2015

• Mass Spectrometry Letters
• /
• 제2권3호
• /
• pp.65-68
• /
• 2011

The effects of column length and particle size on the efficiency of separation and characterization of phospholipids (PLs) are investigated using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS-MS). Since PLs are associated with cell proliferation, apoptosis, and signal transduction, it is of increasing interests in lipidomics to establish reliable analytical methods for the qualitative and quantitative profiling of PLs related to biomarker development in adult diseases. Due to the complexity of PLs, the preliminary separation of PLs is necessary prior to MS analysis. In this study, length of capillary column and the particle size of reversed phase ($C_{18}$) packing materials are varied to find a reliable condition for the high speed and high resolution separation using 8 PL standard mixtures. From experiments, it was found that a capillary column of nLC-ESI-MS-MS analysis for PL mixtures can be minimized to a 5 cm long pulled tip column packed with 3 ${\mu}m$ $C_{18}$ particles without losing resolution.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권10호
• /
• pp.4369-4376
• /
• 2015

Background: To investigate in-vitro antagonistic effect of low-dose liquiritigenin on gemcitabine-induced capillary leak syndrome (CLS) in pancreatic adenocarcinoma via inhibiting reactive oxygen species (ROS)-mediated signalling pathways. Materials and Methods: Human pancreatic adenocarcinoma Panc-1 cells and human umbilical vein endothelial cells (HUVECs) were pre-treated using low-dose liquiritigenin for 24 h, then added into gemcitabine and incubated for 48 h. Cell viability, apoptosis rate and ROS levels of Panc-1 cells and HUVECs were respectively detected through methylthiazolyldiphenyl-tetrazoliumbromide (MTT) and flow cytometry. For HUVECs, transendothelial electrical resistance (TEER) and transcellular and paracellular leak were measured using transwell assays, then poly (ADP-ribose) polymerase 1 (PARP-1) and metal matrix proteinase-9 (MMP9) activity were assayed via kits, mRNA expressions of p53 and Rac-1 were determined through quantitative polymerase chain reaction (qPCR); The expressions of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and PARP-1 were measured via western blotting. Results: Low-dose liquiritigenin exerted no effect on gemcitabine-induced changes of cell viability, apoptosis rate and ROS levels in Panc-1 cells, but for HUVECs, liquiritigenin ($3{\mu}M$) could remarkably elevate gemcitabine-induced decrease of cell viability, transepithelial electrical resistance (TEER), pro-MMP9 level and expression of ICAM-1 and VCAM-1 (p<0.01). Meanwhile, it could also significantly decrease gemcitabine-induced increase of transcellular and paracellular leak, ROS level, PARP-1 activity, Act-MMP9 level, mRNA expressions of p53 and Rac-1, expression of PARP-1 and apoptosis rate (p<0.01). Conclusions: Low-dose liquiritigenin exerts an antagonistic effect on gemcitabine-induced leak across HUVECs via inhibiting ROS-mediated signalling pathways, but without affecting gemcitabine-induced Panc-1 cell apoptosis. Therefore, low-dose liquiritigenin might be beneficial to prevent the occurrence of gemcitabine-induced CLS in pancreatic adenocarcinoma.