• 한국임상수의학회지
• /
• 제32권2호
• /
• pp.154-157
• /
• 2015

개에서 설사를 일으키는 주요 원인이 되는 장내 병원균으로 Salmonella spp., Shigella spp., Campylobacter spp., Clostridium spp.가 있다. 이들 세균은 배양으로 검출이 어렵다. 본 실험에서는 Salmonella spp., C. coli, C. jejuni, 그리고 Cl. perfringens를 신속하고 민감하게 검출할 수 있는 방법을 고안하였다. 정상견 71마리와 설사증상이 있는 66마리에서 수집한 분변 시료에서 장내 병원균의 유병률을 알아보고자 하였다. 장내 병원균은 실시간 중합효소 연쇄 반응 분석을 이용하여 검출하였다. 설사변에서 Salmonella spp., C. coli, C. jejuni, Cl. Perfringens는 정상변보다 검출률이 높았다. 개발한 다중실시간 중합효소연쇄반응은 분변시료의 병원균 존재 및 양 또는 기타 고유 서열을 확인하는데 유용하였다.

• 대한수의학회지
• /
• 제27권2호
• /
• pp.245-251
• /
• 1987

A total of 145 strains of thermophilic Campylobacter spp. isolated from the fecal specimens of 108 cattle, 120 pigs and 104 chickens. The isolation rates of Campylobacter jejuni from cattle, pigs and chickens were 36.1%, 38.3% and 28.8%, respectively. In the biotyping of 115 strains of C. jejuni, 49.6% were belonged to biotype I, 33.9% biotype II, 10.4% biotype IV and 6.1 % biotype III. Twenty-eight strains of C. coli were 78.6% of biotype I, 21.4% biotype II. Two strains of C. laridis belonged to biotype I and II. One hundred of 105 C. jejuni cultures were typable serologically and represented 13 serogroups Serotype 4, 5, 26, 27 and 36 were encountered most frequently. Eighteen of 23 C. coli cultures were typable serologically and represented 6 serogroups. Serotype 8, 20, 21 and 31 were encountered most frequently. In the comparison of frequency of serotype between animal species, serotypes 4, 30, 5, 26 and 27 were encountered relatively common in the cattle source isolates, serotypes 26 and 36 in the pigs, and 36 and 17 in the chickens. The serotypes of C. coli encountered most frequently were serotype 8 and 31.

• 대한수의학회지
• /
• 제29권3호
• /
• pp.303-313
• /
• 1989

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campyllobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Campylobacter spp. were examined. The results were as follows. 1. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60% of the isolates were noted to harbor plasmid DNA. Plasmid occurrencer ate from pigs, chickens and cattle were 76.2%, 61.7% and 37.7%, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. 2. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10~1,000. 3. Tetracycline resistant plasmids of C jejuni were transferrable to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E coli and Vibrio cholerae. 4. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation.

• 한국축산식품학회지
• /
• 제43권1호
• /
• pp.73-84
• /
• 2023

Campylobacteriosis is a common cause of gastrointestinal disease. In this study, we suggest a general strategy of applying gold nanoparticles (AuNPs) in colorimetric biosensors to detect Campylobacter in chicken carcass. Polymerase chain reaction (PCR) was utilized for the amplification of the target genes, and the thiolated PCR products were collected. Following the blending of colloid AuNPs with PCR products, the thiol bound to the surface of AuNPs, forming AuNP-PCR products. The PCR products had a sufficient negative charge, which enabled AuNPs to maintain a dispersed formation under electrostatic repulsion. This platform presented a color change as AuNPs aggregate. It did not need additional time and optimization of pH for PCR amplicons to adhere to the AuNPs. The specificity of AuNPs of modified primer pairs for mapA from Campylobacter jejuni and ceuE from Campylobacter coli was activated perfectly (C. jejuni, p-value: 0.0085; C. coli, p-value: 0.0239) when compared to Salmonella Enteritidis and Escherichia coli as non-Campylobacter species. Likewise, C. jejuni was successfully detected from artificially contaminated chicken carcass samples. According to the sensitivity test, at least 15 ng/μL of Campylobacter PCR products or 1×10³ CFU/mL of cells in the broth was needed for the detection using the optical method.

• 대한수의학회지
• /
• 제29권3호
• /
• pp.291-301
• /
• 1989

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campylobacter spp., isolation of etiological agent was carried out. Isolated Campylobacter spp. were biotyped, serotyped and the susceptibility of the isolates to antimicrobial agents were examined. Th results were as follows. 1. Isolation rates of Campylobacter spp. from 649 fecal materials of 208 cattle, 300 pigs and 141 chickens were 25.5%, 23.7% and 38.3%, respectively. 2. The majority of the 130 isolates of C jejuni was classified as biotype I(50.6%) and biotype II (34.6%). Most of the 46 isolates of C coli were biotype I (71.7%). 3. Isolated C jejuni strains showed 14 different serotype, and serotype 4, 26, 36 were most frequent. Isolated C coli strains showed 5 different serotype and serotype 31 and 21 were relatively common. 4. Isolated Campylobacter spp. were highly susceptible to nalidixic acid, amikacin, gentamicin, colistin and chlorampehnocol.

• 미생물학회지
• /
• 제30권3호
• /
• pp.232-238
• /
• 1992

Campylobacter jejuni 에 열처리를 했을 때 그들의 생존성 및 열충격 단백질 합성의 양상과 더불어, dnaK 와 groESL 유전자를 이용하여 C. jejuni 의 열충격 유전자를 검출하여 그 특성을 E. coli 의 열충격 유전자와 비교하였다. C. jejuni 의 열충격 단백질은 48.deg.C 에서 가장 잘 발형되었으며, 48.deg.C 에서 30 분간의 처리중 세포들의 생존율은 떨어지지 않았다. C. jejuni 의 열충격 단백질로서의 Hsp90, Hsp66, Hsp60 이 합성되는 것을 SDS-PAGE 및 방사선사진법을 통해 확인하였다. dnaK 와 groESL 을 DNA 탐침자로 이용하여 Southern hybridization 한 결과, C. jejuni 의 열충격 유전자도 groESL 과 dnaK 유전자와 상동성을 가진 염기서열을 가지고 있었으나, 두 균주사이에는 열충격유전자를 내포하고 있는 DNA 상에서 제한효소의 절단부위에 차이가 있었다.

• 한국동물위생학회지
• /
• 제40권2호
• /
• pp.101-105
• /
• 2017

The purpose of this study was to investigate prevalence and antimicrobial resistance patterns of Campylobacter spp. from duck feces in northern area of the Gyeongnam province, Korea. Samples of 121 duck feces were taken from April to December 2014 for this survey. Samples were examined by bacteria isolation and reverse transcriptase-polymerase chain reaction assay for detection of Campylobacter spp. Campylobacter were isolated in 37 samples (30.6%). Among these samples, C. jejuni and C. coli were isolated in 35 samples and 2 samples, respectively. Minimum inhibitory concentration (MIC) test is performed to investigate antimicrobial resistance patterns of Campylobacter spp. C. jejuni were resistant to ciprofloxacin (85.7%), nalidixic acid(82.9%), tetracycline (77.1%), gentamicin (57.1%), azithromycin (40.0%), clindamycin (34.3%), erythromycin (22.9%), and florfenicol (8.6%). These data support a database of pollution and antimicrobial resistance of Campylobacter spp. from duck feces and provide a basic information of reducing the secondary damage of antibiotic misuse.

• 한국식품위생안전성학회지
• /
• 제14권4호
• /
• pp.327-332
• /
• 1999

1996년 3월부터 1998년 10월간에 경남지방 시판 냉장육 888건, 냉동포장육 222건 및 수입 냉동육 117건의 시료로부터 식중독관련 병원균의 분포와 혈청형을 조사하였다. 식중독균의 분리률은 Staphylocorcus aureus. Campylobarter jejuni/coli, Listeria monocytogenes 및 Salmonella spp 순으로 높았고, Escherichia coli O157:H7은 전시료를 통해서 분리되지 않았다. C. jejuni /coli는 냉장육에서 높은 오염률을 나타내고, 냉동포장육에서는 거의 분리되지 않는데 반해서 L. monocytogenes는 냉장육에 이해서 냉동포장 계육에서 높은 분리율을 나타낸 것은 매우 흥미로운 일이다. 분리균의 혈청형 분포는 Sta. aureus의 경우 대부분이 enterotoxin type C와 D였고, Salmonella spp는 돈육유래균에서 모두 A group이었으며, 계육유래균은 대부분 B와 D group이었다. L. monocytogenes는 계육유래균의 대부분이 type 1 이었고, type 4는 소수로 분리되었다.

• 대한수의학회지
• /
• 제28권1호
• /
• pp.67-73
• /
• 1988

This study was conducted to isolate etiological agents from the 103 scouring piglets in Gyeongnam area and also carried out antimicrobial drug susceptibility test and epidemiogical served. The incidence of scouring piglet was most prevalent as 81.6% in the age of 2 to 4 weeks after birth, while the rate was less than 10% in the age of 5 to 6 weeks and under 1 week after birch. When compared the isolation frequency of the each etiological agent, enteropathogenic E. coli was most prevalent as 46.6%, thermophilic Campylobacter 26.2% and Salmonellae was 8.7% in order. In the OK serotyping for 117 isolates of enteropathogenic E. coli, type 0141 : K85 (20.5%), 0157:K88ac(14.5%), 0138:K81 and 0149:K91 (13.3%) were encountered most frequently. In the biotyping for 27 isolates of thermophilic Campylobacter, most strains of C. jejuni were belong to type I (50.0%) and II (25.0%), and most strains of C. coli were belong to biotype I (78.9%). In the serotyping for 9 strains of Salmonellae, 3 strains were grouped as D, 2 strains as C. and each 1 strain was group B and E. The other 2 strains were untypable. The 117 isolates of enteropathogenic E. coli were resistant more than 90% to erythromycin, penicillin, tetracycline and streptomycin, wherease about 90% of the isolates were sensitive to kanamycin and gentamicin. In the case of Salmonellae, all of the isolates were resistant to penicillin, but about 89% of the isolates were sensitive to gentamicin and colistin. All of C. jejuni and C. coli isolates were resistant to cephalothin, but more than 89% of C.jejuni and C. coli were sensitive to kanamycin and gentamicin.

• 한국동물위생학회지
• /
• 제38권3호
• /
• pp.167-171
• /
• 2015

Pathogenic microorganisms were monitored on the chicken carcasses in slaughterhouse of Jeonbuk area. It was conducted to evaluate the microbiological quality on 204 chicken carcasses. Staphylococcus (S.) aureus was isolated in largest number and its ration was 41.2%, Salmonella spp. 6.4%, Campylobacter (C.)jejuni 7.4%, C. coli 7.4%. Serotype of Salmonella (S.) spp. was identified as S. Infantis 46.1%, S. Enteritidis 23.1%, S. Typhimurium 7.7%, S. Montevideo 7.7%. In breed chickens, Salmonella spp. was detected broiler 4.1%, white semi-broiler 8.0% Korean native chicken 12.0%. C. jejuni was isolated broiler 7.4%, white semi-broiler 12.0%, Korean native chicken 0%, C. coli, broiler 7.4%, white semi-broiler 0%, Korean native chicken 18.1% and S. aureus, broiler 38.8%, white semi-broiler 40.0%, Korean native chicken 51.5%.