• Journal of Nutrition and Health
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• v.32 no.4
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• pp.337-352
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• 1999

The effects of dried leaf powders and ethanol extract of persimmon, green tea and pine needle on lipid metabolism, lipid peroxidation and antioxidative activity were investigated in rats. Forty-nine male Spargue-Dawley rats weighing 107.8$\pm$1.8g were blocked into seven groups according to body weight. Rats were raised for four weeks with diets containing either 5%(w/w) dried leaf powders of one of three different Korea traditional teas, persimmon(Diospyros kaki Thunb), green tea(Camellia Sinensis O.Ktzc)or pine needle(pinus Koreansis Sieb. Et Zucc), or ethanol from equal amounts of each dried tea powder. Food intake, weight gain, food efficiency ratio, and weights of liver, kidney and epididymal fat were significantly higher in the green-tea-powder group, and significantly lower in the pine-needle-powder and pine-needle-extract groups. Persimmon-leaf powder was found to decrease plasma total lipid, triglyceride and cholesterol concentration by increasing fecal total lipid triglyceride and cholesterol excretions. Liver cholesterol concentration was significantly lower in the green-tea and pine-needle-extract groups. Red-blood-cell superoxide dismutase(SOD) and glutathione peroxidase(GSH-px) activities were significantly increased in rats fed green-tea extract. Liver SOD activity was increased in rats fed pine-needle powder or extract, and liver GSH-px activity was increased in rats fed greentea powder. Plasma and liver thiobarbituric acid reactive substance(TBARS) concentration were both decreased in rats fed dried leaf posers or extracts of persimmon or green tea. It is believed that high vitamin E levels in persimmon leaf, and high flavonoid, beta-carotene and vitamin C levels in green tea effectively inhibited lipid peroxidation. In conclusion, persimmon and green tea leaves were effective in lowering lipid levels and inhibiting lipid peroxidation in animal tissue, while pine needles were effective and lowering body weight gain. From these results, persimmon and green tea leaves can be recommended in the treatment and prevention of chronic discorders such as cardiovascular disease, cancer and aging. As ethanol extracts from these teas were also effecitive in lowering tissue lipid levels and inhibiting lipid peroxidation, we recommend the use of discarded tea grounds for this.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.6
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• pp.774-779
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• 2006

The commercial green tea leaves were packaged with polyethylene film and irradiated by electron beam at doses of 0, 5, 10, 20, and 30 kGy. After irradiation, green teas were prepared by soaking the leaves in water (1 g/100 mL) at $75^{\circ}C$ for 10 min, and the physicochemical characteristics of green tea were determined. Electron beam irradiation decreased total phenol contents (TPC), total flavanol contents (TFC), ascorbic acid contents (AAC). Irradiation at dose of 20 kGy decreased TPC, TFC, and AAC from 223.46, 32.50, and 6.03 mg/g to 202.88, 31.16, and 5.57 mg/g, respectively, compared with non irradiated control. Electron beam irradiation also decreased catechins, caffeine, and nitrite scavenging activity of green tea. However, the changes of overall color and radical scavenging activity of irradiated green tea were negligible.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.302-310
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• 2016

A study aimed at identifying putative drought responsive genes that confer tolerance to water stress deficit in tea plants was conducted in a 'rain-out shelter' using potted plants. Eighteen months old drought tolerant and susceptible tea cultivars were each separately exposed to water stress or control conditions of 18 or 34% soil moisture content, respectively, for three months. After the treatment period, leaves were harvested from each treatment for isolation of RNA and cDNA synthesis. The cDNA libraries were sequenced on Roche 454 high-throughput pyrosequencing platform to produce 232,853 reads. After quality control, the reads were assembled into 460 long transcripts (contigs). The annotated contigs showed similarity with proteins in the Arabidopsis thaliana proteome. Heat shock proteins (HSP70), superoxide dismutase (SOD), catalase (cat), peroxidase (PoX), calmodulinelike protein (Cam7) and galactinol synthase (Gols4) droughtrelated genes were shown to be regulated differently in tea plants exposed to water stress. HSP70 and SOD were highly expressed in the drought tolerant cultivar relative to the susceptible cultivar under drought conditions. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against water stress deficit by tea. The knowledge generated can be further utilized to better understand molecular mechanisms underlying stress tolerance in tea.

• Korean journal of food and cookery science
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• v.28 no.3
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• pp.319-326
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• 2012

This study was designed to investigate the properties related to browning of hot water and 80% ethanol extracts of green tea (Camellia sinensis) and propolis. The extracts were assessed for polyphenol oxidase (PPO) inhibitory activity and total phenolic and flavonoid contents. In addition, apples were cut into 15 mm thick slices and dipped for 1 min in 0.5% of the green tea extracts and the propolis solutions and stored at room temperature for 24 h. The PPO inhibitory activity of the green tea extracts was better than that of the propolis. The highest DPPH free radical scavenging activity (90.81%), total phenol contents (170.42 mg/mL) and the metal chelating effects (45.93%) were measured in the green tea hot water extracts. The mineral content of the green tea water extracts was 69,328.44 ppm potassium and 2,409.42 ppm magnesium. After 24 h, the ${\Delta}E$ value of the apple slices treated with the green tea water extracts was the lowest (1.35). The antibrowning effects of the green tea extracts were higher than those of propolis in the apple slices.

• Korean Journal of Pharmacognosy
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• v.39 no.2
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• pp.155-163
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• 2008

This study was carried out to search the natural antioxidants from the 36 medicinal plants. $IC_{50}$ values of linoleic acid peroxidation inhibition to the BHA, BHT and ${\alpha}-tocopherol$ showed $7.2{\mu}g/ml$, $7.3{\mu}g/ml$, and $26.6{\mu}g/ml$, but hexane extract of Zingiber officinale showed $13.2{\mu}g/ml$, EtOAc extracts of Phellinus linteus, Morus alba, Zingiber officinale and butanol extract of Diospyros kaki showed $15.9{\mu}g/ml$, $16.9{\mu}g/ml$, $20.1{\mu}g/ml$, and $21.5{\mu}g/ml$ respectively. $IC_{50}$ values of DPPH radical scavenging activity of BHA, BHT and $\alpha$-tocopherol showed $14.9{\sim}27.7{\mu}g/ml$, but EtOAc extract of Paeonia suffruticosa showed the lowest $6.5{\mu}g/ml$ than those of other extracts, EtOAc extracts of Camellia sinensis, Salvia miltiorrhiz, and Phellinus linteus showed $8.0{\mu}g/ml$, $11.5{\mu}g/ml$, and $13.0{\mu}g/ml$ respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.29-39
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• 2011

This study aimed to select evergreen broad-leaved trees (EBT) that can be produced and planted in Korea by analyzing the current use of the trees for landscaping in the Kyushu area of Japan, a warm temperate region. The results revealed the total production of EBT was higher than that of conifers and due to the suitable growth environment of the area and the subsequent high demand for them. The landscape tree production methods in Japan were divided into container nursery and outdoor nursery, and the uses and species of the trees varied depending on the method; a variety of native species were produced in container nursery rather than in outdoor nursery, whereas trees used as ornamental, gardening, and shade plants were produced in outdoor nursery rather than in container nursery. The results also showed that in Fukuoka, a major city in Kyushu that is adjacent to Korea, the planting rate of EBT, used as ornamental, gardening, and shade plants, was higher than that of deciduous broad-leaved trees and conifers. In the city, the planting rate of Cinnamomum camphora was the highest, Elaeocarpus sylvestris var. ellipticus, Ternstroemia gymnanthera, Ilex rotunda, Quercus glauca, Camellia sasanqua, Eurya emarginata, Pittosporum tobira, Raphiolepis indica var. umbellata, Hedera rhombea, Kadsura japonica, Trachelospermum asiaticum var. asiaticum. These species were verified in Kyushu area for their application as landscape trees and are expected to serve as landscape trees in Korea if the planting areas of them are expanded by global warming, urban heat island and regional microclimate.

• Korean Journal of Environment and Ecology
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• v.21 no.2
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• pp.141-148
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• 2007

To investigate the forest community structure ranging from 200 meters to 1,350 meters above sea level at Donnaeko valley of Mt. Hallasan in 2006, 15 plots were surveyed. According to the classification analysis by TWINSPAN, the plant communities were divided into five groups of Castanopsis sieboidii community, Castanopsis sieboldii - Quercus acuta community, mixed forest, Carpinus laxiflora - Quercus serrata community and deciduous broad-leaved forest. 22 species of evergreen broad-leaved trees such as Castanopsis sieboldii, Quercus acuta, Distylium racemosum, Camellia japonica, Eurya japonica, Ligustrum lucidum, Ilex crenata, Daphnipyllum macropodum, etc. were growing at Donnaeko valley. According to the attitudinal distribution of evergreen broad-leaved trees, Castanopsis sieboidii was a dominant species distributed from 200 meters to 350 meters above sea level, Castanopsis sieboldii and Quercus acuta were dominant species distributed from 400 meters to 600 meters above sea level and Quercus acuta was a dominant species distributed from 660 meters to 700 meters above sea level. Ilex crenata, Daphniphyllum macropodum, Elaeagnus glabra were distributed up to 1,350 meters above sea level in Donnaeko.

• Journal of the Korean Wood Science and Technology
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• v.24 no.1
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• pp.27-33
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• 1996

The aim of this research was to investigate the chemical components of C30 compounds, especially triterpenoids in Korean native mistletoes of Korthalsella japonicus Engler parasiting to Camellia japonica L., Viscum album var. coloratum (Kom.) Ohmi, to Quercus acutissima Carruth. and Loran-thus yadoriko Sieb. to Neolitsea sericea (BI.) Koidz. For the identification of triterpenoidal components, alkaline hydrolyzates of mistletoes meals were analyzed by TLC, GC, and GC/MS. The content of oleanolic acid and ursolic acid derivatives were highest in K. japonica. In V. album, there was no big difference between leaves and twigs in content. but oleanolic acid in leaves. and olean-12-en-$3{\beta}$-ol and lup-20(29)-en-3-one in twigs were prominent. Similiar to V. album in L. yadoriki there was no difference between leaves and twigs in content, and both olean-12-en-$3{\beta}$-ol, lup-20(29)-en-3-one and urs-12-en-$3{\beta}$-ol in leaves, lup-20(29)-en-3-one in twigs were abundant. Triterpenoids as olea-12-en-$3{\beta}$-ol, lupe-20(29)-en-3-one, 3-oxo-urs-12-en-24-oic acid, and $21{\beta}$-A'-neogam-macer-22(29)-en-3-ol acetate were common in all samples tested. whereas ursolic acid only in P. japonicus and ursenol in L. yadoriki were detected. And P. japonicus had the largest number of triterpenoids and showed the highest in biological activity. So it is noted that Korean mistletoes tested in the study had three types of triterpenoid, oleanane, lupane, and ursane, irrespective of hosts, sampling positions and species.

• Korean journal of food and cookery science
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• v.24 no.1
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• pp.11-15
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• 2008

In searching for yeast to be utilized as biocontrol agents, a single yeast strain was isolated from Camellia sinensis based on its morphological, cultural, physiological, and biochemical properties, as well as by molecular techniques. This single strain was pink to red in color and designated as strain JY-1. The effects of temperature, pH, NaCl concentration, and ethanol concentration on the growth of the JY-l strain were examined for the JY-1. Growth occurred at temperatures ranging from 20 to $35^{\circ}C$, and between pH 3.0 and 12.0, with optimal growth at $25-30^{\circ}C$ and pH 5.0. The yeast also grew in the presence of 0-2% (w/v) NaCl and 0-4% (v/v) EtOH. The isolate was further classified based on biochemical characteristics using the VITEK system. The biochemical data obtained using this system were similar to those of Rhodotorula glutinis/Rhodotorula mucilaginosa (exhibiting a 93% matching level). Molecular phylogenetic analysis based on l8S rDNA sequences indicated that the yeast represented a basidiomycetous species, and its highest degree of sequence similarity was with Rhodosporidium azoricum, strain JCM11251 (99%).

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.237-248
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• 2008

The chemical composition of the volatile constituents from broad-leaved indeciduous trees was determined by GC and GC/MS spectrometric analysis with the aid of NBS, Wiley Library and RI indice searches. The major constituents were identified as hotrienol (9.21%) from Ligustrum japonicum flowers, undecane (32.12%) from Pittosporum tobira leaves, 1,8-cineole (45.32%) from Illicium religiosum leaves, ${\gamma}$-terpinene (15.62%) from Neolitsea aciculata leaves, 1,8-cineole (14.66%) from Cinnamomum japonicum leaves, 2-acetyl-5-methylfuran (54.51%) from Camellia sinensis leaves, and ${\gamma}$-elemene (18.59%) from Dendropanax morbifera leaves.