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Antioxidative Activities and Antibrowning Effects of Green Tea Extracts and Propolis

녹차 추출물 및 프로폴리스의 항산화 및 갈변억제 효과

  • Published : 2012.06.30

Abstract

This study was designed to investigate the properties related to browning of hot water and 80% ethanol extracts of green tea (Camellia sinensis) and propolis. The extracts were assessed for polyphenol oxidase (PPO) inhibitory activity and total phenolic and flavonoid contents. In addition, apples were cut into 15 mm thick slices and dipped for 1 min in 0.5% of the green tea extracts and the propolis solutions and stored at room temperature for 24 h. The PPO inhibitory activity of the green tea extracts was better than that of the propolis. The highest DPPH free radical scavenging activity (90.81%), total phenol contents (170.42 mg/mL) and the metal chelating effects (45.93%) were measured in the green tea hot water extracts. The mineral content of the green tea water extracts was 69,328.44 ppm potassium and 2,409.42 ppm magnesium. After 24 h, the ${\Delta}E$ value of the apple slices treated with the green tea water extracts was the lowest (1.35). The antibrowning effects of the green tea extracts were higher than those of propolis in the apple slices.

Keywords

References

  1. Abe Y, Umemura S, Sugimoto KL, Kirawa N, Kato Y, Yokoyama N, Yokoyama T, Iwai J, Ishii M. 1995. Effects of green tea rich in ${\gamma}$-aminobutyric acid on blood pressure of dahl salt-sensitive rats. American J Hypertension 8:74-79 https://doi.org/10.1016/0895-7061(94)00141-W
  2. Ahvenainen R. 1996. New approaches in improving the shelf life of minimally processed fruit and vegetables. Trends Food Sci Technol 7:179-186 https://doi.org/10.1016/0924-2244(96)10022-4
  3. AOAC. 1995. Official methods of analysis 16th ed. Association of Official Analytical Chemists, Washington, pp 3-20
  4. Bonvehi JS, Coll FV, Jorda RE. 1994. The composition, active components bacteriostatic activity of propolis in dietetics. J Am Oil Chem Soc 71:529-532 https://doi.org/10.1007/BF02540666
  5. Burdock GA. 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 35:347-376
  6. Chiao C, Carothers AM, Grunberger D, Solomon G, Preston A, Barrett JC. 1995. Apotosis and altered redox state induced by caffeic acid phenethyl ester(CAPE) in transformed rat fibroblast cell. Cancer Res 55:3576-3683
  7. Choi EH, Jung DS, Cho NS, Shim YH. 1987. Characteristics and inhibition of polyphenol oxidase from fuji apples. J Korean Soc Appl Chem 30:278-284
  8. Chung HJ. 2010. Antioxidative activities of different part extracts of Physalis alkekengi var. francheti (Winter Cherry). Korean J Food Preserv 17:867-873
  9. Chung HS, Peter T, Moon KD. 2008. Combined effects of vanillin and antibrowning agent on changes of quality and microorganisms in fresh-cut apples. Korean J Food Preserv 15:385-389
  10. Dennis D, Miller JW. 1998. Enzymatic browning. In: Kinetics of tyrosinase. Food Chem. A Laboratory Manual Edition 1. pp 44-49
  11. Donard VS. 1995. Marketing lightly processed fruits and vegetables. Hortsci 30:15-17
  12. Florence CRF, Pascale MG, Jacques JN. 1992. Cysteine as an inhibitor of enzymatic browning. 2 Kinetic studies. J Agric Food Chem 40:2108-2113 https://doi.org/10.1021/jf00023a014
  13. Greenaway W, May J, Scaysbrook T, Whatley FR. 1991. Identification by gas chromatography-mass spectroscopy of 150 compounds in propolis. Zeitschrift fuer Naturforschung 46:111-121
  14. Gulcin I. 2006. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology 21:213-220
  15. Hwang TY, Moon GD. 2006. Quality characteristics of fresh-cut potatoes with natural antibrowning treatment during storage. Korean J Food Sci Technol 38:183-187
  16. Jeong CH, Bae YI, Lee HJ, Shim KH. 2003. Chemical components of propolis and its ethanolic extracts. J Korean Soc Food Sci Nutr. 32:501-505 https://doi.org/10.3746/jkfn.2003.32.4.501
  17. Kim CT, Lee SJ, Hwang JK, Kim CJ, Ahn BH. 1997. Effect of propolis addition on the shelf-life and staling of white bread. Korean J Food Sci Technol 29:982-986
  18. Kim DM. 1999. Extension of freshness of minimally processed fruits and vegetables. Kor J Hort Sci Technol 17:790-795
  19. Kim DY, Rhee CO, Kim YB. 1981. Characteristics of polyphenol oxidase from garlic (Allium sativam L.). J Korean Agric Chem Soc 24:167-173
  20. Kim EJ, Lee HJ, Kim HJ, Nam HS, Lee MK, Kim HY, Lee JH, Kang YS, Lee JO, Kim HY. 2005. Comparison of colorimetric methods for the determination of flavonoid in propolis extract products. Korean J Food Sci Technol 37:918-921
  21. Kim HK, Choi YJ, Kim KH. 2002. Functional activities of microwave-assisted extracts from Flammulina velutipes. Korean J Food Sci Technol 34:1013-1017
  22. Kim JK, Cha WS, Park JH, Oh SL, Cho YJ, Chun SS, Choi C. 1997. Inhibition effect against tyrosinase of condensed tannins from Korean green tea. Korean J Food Sci Technol 28:173-177
  23. Kim MJ, Rhee SJ. 1994. Effects of Korean green tea, oolong tea and black tea beverage on the removal of cadmium in rat. J Korean Soc Food Nutr 23:784-791
  24. Kim SH, Kim IH, Kang BH, Lee KH, Lee SH, Lee DS, Cho SK, Hur SS, Kwon TK, Lee JM. 2009. Optimization of ethanol extraction conditions from propolis (a bee product) using response surface methodology. Korean J Food Preserv 16:908-914
  25. Kim SJ, Hwang JH, Han KH, Cho JY, Ma SJ, Kim DW, Moon JH, Park KH, Kim SJ. 2010. Antimicrobial activities of green tea extract on Vibrio parahaemolyticus. J Korean Tea Soc 16:82-87
  26. Kim YH, Koh JB. 1985. The mineral contents of green tea made with different drawing conditions. J Korean Soc Food Nutr 14:289-295
  27. Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christove R, Popov S. 1999. Antibacterial, antifungal and antiviral activity of different geographic origin. J Ethnopharmacol 64:2358-240
  28. Lee GC, Ahn SC. 1997. Inhibition effect of several cereal extracts on enzymatic browning. Korean J Soc Food Sci 13:390-395
  29. Lee HJ, Bae YI, Jeong CH, Shin KH. 2005. Biological activities of various solvent extracts from propolis. J Korean Soc Food Sci Nutr 34:1-7 https://doi.org/10.3746/jkfn.2005.34.1.001
  30. Lee YC, Hwang KH, Han DH, Kim SD. 1997. Composition of Opuntia ficus-indica. Korean J Food Sci Technol 5:847-853
  31. Liao S, Umwkita Y, Guo J, Kokontis JM, Hipacca RA. 1995. Growth inhibition and regression of human prostate and breast tumors in athymic mice by tea epigallocatechin gallate. Cancer Lett 96:239-243 https://doi.org/10.1016/0304-3835(95)03948-V
  32. Lim DK, Choi U, Shin DH, Jeong YS. 1994. Antioxidative effect of propolis extract on palm oil and lard. Korean J Food Sci Technol 26:622-626
  33. Lim JH. 2005. The activity control of plant polyphenol oxidase and antibrowning effect on fresh-cut produces by green tea (Camellia sinesis) catechins. Doctorate thesis. The Kyungbook National University. pp 3
  34. Lin SD, Liu EH, Mau JL. 2008. Effect of different brewing methods on antioxidant properties of steaming green tea. Food Sci Technol 41:1616-1623
  35. Lu MJ, Chen C. 2008. Enzymatic modification by tannase increase the antioxidant activity of green tea. Food Res Int 41:130-137 https://doi.org/10.1016/j.foodres.2007.10.012
  36. Mizno M. 1989. Propolis-or its extract-containing resin compositions. Japanese Patent JP 01 245 058 [89 245 0587]
  37. Mondy NI, Munski CB. 1993. Effect of boron on enzymatic discoloration and phenolic and ascorbic acid contents of potatoes. J Agric Food Chem 41:554 https://doi.org/10.1021/jf00028a009
  38. Muramatsu K, Fukuyo M and Hara Y. 1986. Effect of green tea catechins on plasma cholesterol level in cholesterol fed rats. J Nutr Sci Vitamonol 32:613-622 https://doi.org/10.3177/jnsv.32.613
  39. No JK, Soung DY, Kim YJ, Shim KH, Jun YS, Rhee SH, Yokozawa T, Chung HY. 1999. Inhibition of tyrosinase by green tea components. Life Sci 65:241-246
  40. Park CO. 1996. Antioxidant activity of boiling water extracts obtained from green tea. Doctorate thesis. The Pusan National University. pp 26-30
  41. Park EH, Kim SH, Park SS. 1996. Antiinflammatory activity of propolis. Arch Pharm Res 19:337-341 https://doi.org/10.1007/BF02976375
  42. Park SY, Hwang TY, Kim JH, Moon KD. 2001. Quality changes of minimally processed lotus root (Nelumbo nucifera) with browning inhibitors. Korean J Postharvest Sci Technol 8:164-168
  43. Pruidze GN, Grigorashvili GZ. 1977. A review the enzymes of tea and their role in tea making. J Food Biol 8:243-279
  44. Pyo YH, Lee TC, Logendra L, Rogen RT. 2004. Antioxidant activity and phenolic compounds of Swiss chard (Betavulgaris subspecies cicla) extracts. Food Chem 85:19-26 https://doi.org/10.1016/S0308-8146(03)00294-2
  45. Ramatahman N, Osawa T, Ochi H, Kawakishi S. 1995. The contribution of plant food antioxidants to human health. Trends Food Sci Tech 6:75-82 https://doi.org/10.1016/S0924-2244(00)88967-0
  46. Rhi JW, Shin HS. 1993. Antioxidant effect of aqueous extract obtained from green tea. Korean J Food Sci Technol 25:759-763
  47. Ryu JM, Park YJ, Choi SY, Hwang TY, Oh DH, Moon GD. 2003. Browning inhibition and quality characteristics of minimally processed mushroom (Agaricus bisporus Sing) using extracts from natural materials during storage. Korean J Food Preserv 10:11-15
  48. Santhosh KT, Swarnam J, Ramadasan K. 2005. Potent suppressive effect of green tea polyphenols on tobacco-induced mutagenicity. Phytomedicine 12:216-220 https://doi.org/10.1016/j.phymed.2003.09.011
  49. Santoru K, Kazuhiko T, Nori M, Jinetsu U. 2002. Antioxidative activitiy of apple skin or flesh extracts associated with fruit development on selected apple cultivars. Scient Horticul 96:177-185 https://doi.org/10.1016/S0304-4238(02)00127-9
  50. Sapers GM, Miller RL. 1992. Enzymatic browning control in potato with ascorbic acid-2-phosphate. J Food Sci 57:1132-1135 https://doi.org/10.1111/j.1365-2621.1992.tb11281.x
  51. Seong HM, Seo MS, Kim SR, Park YK, Lee YT. 2002. Characteristics of barley polyphenol extract (BPE) separated from pearling by-products. Korean J Food Sci Technol 34:775-779
  52. Shigenori K, Tomoko H, Tsutomu N, Grange JM. 1990. Antibacterial properties of propolis (bee glue). J Royal Soc Med 83:159-160
  53. Shigenori K, Tomoko H, Tsutomu N. 2004. Antioxidant activity of propolis of various geographic origins. Food Chem 84:329-339 https://doi.org/10.1016/S0308-8146(03)00216-4
  54. Tan NH, Wong KC, Lumen BO. 1984. Relationship of tannin levels and trypsin inhibitor activity with the in vitro protein digestibilities of raw and heat-treated winged bean. J Agric Food Chem 32:819-822 https://doi.org/10.1021/jf00124a030
  55. Vamons-Vigyazo L. 1981. Polyphenol oxidase and peroxidase in fruits and vegetables. CRC Crit Rev Food Sci Nutr 15:49-53 https://doi.org/10.1080/10408398109527312
  56. Wang H, Helliwell K. 2001. Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography. Food Res Int 34:223-227 https://doi.org/10.1016/S0963-9969(00)00156-3
  57. Weller A, Sims CA, Matthews RF, Bates RP, Brecht JK. 1997. Browning susceptibility and changes in composition during storage of carambola slices. J Food Sci 62:256-260 https://doi.org/10.1111/j.1365-2621.1997.tb03980.x

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