• Journal of Food Hygiene and Safety
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• v.33 no.2
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• pp.110-117
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• 2018

The aim of this study was to develop an analytical method for the quantification of diazepam residues in fishery products, using liquid and gas chromatography-tandem mass spectrometry (LC-MS/MS and GC-MS/MS). The sample utilized in the study was extracted from the fish sample (crucian carp) using 0.1% formic acid in acetonitrile. For the utilization of the purification process, the dispersive solid phase extraction (dSPE) was used for LC-MS/MS, dSPE and SPE was used for GC-MS/MS, respectively. To be sure, the standard calibration curves showed a good linearity as the noted correlation coefficients, $r^2$ was > 0.99. The average recoveries for accuracy ranged in 99.8~124% for the samples which were fortified at three different levels (0.001, 0.002 and 0.010 mg/kg). The correlation coefficient for the precision effect was measured at a range of 4.01~11.8%. The limit of detection (LOD) for the diazepam analysis was 0.0004 mg/kg, and the limit of the quantification (LOQ) was 0.001 mg/kg. The proposed analytical method was characterized with a high accuracy and acceptable sensitivity to meet the established Codex Alimentarius Commission (CAC/GL71-2009) guideline requirements. We therefore established the optimal analysis method for the determination of diazepam in the fishery products using LC-MS/MS and GC-MS/MS. It would be applicable to analyze the diazepam residues in fishery products in further studies on this subject.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.391-397
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• 2017

Recently, digital image correlation (DIC) techniques have been used to measure dynamic deformation during tensile testing. The standard tensile test method measures the average displacement of the relevant specimen to calculate the true stress-strain curve. Therefore, the validity of the true stress curve is restricted to the stress incurred within the uniform stretching interval, i.e., the maximum stress corresponds to the starting point of the necking deformation. Alternatively, if DIC is used, the effective range of the strain and strain rate can be extended to the breaking point of the tensile specimen, because of the feasibility of measuring the local strain over the entire area of interest. Because of these advantages, many optical 3D measurement systems have been introduced and used in research and industry. However, the conventional 3D measurement systems are exceedingly expensive and time consuming. In addition, these systems have the disadvantage of a very large equipment size which makes their transport difficult. In this study, a 2D image correction method employing a 2D DIC measurement method in conjunction with a numerical analysis method is developed using a smartphone. The results of the proposed modified 2D DIC method yielded higher accuracy than that obtained via the 3D measurement equipment. In conclusion, it was demonstrated that the proposed 2D DIC and calibration methods yield accurate measurement results with low time costs.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.3
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• pp.342-351
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• 2016

This study was carried out to develop an optimized analytical method using high-performance liquid chromatography (HPLC) applied to canthaxanthin, which is not yet designated as a food colorant in Korea, as well as to perform validation and uncertainty evaluation of this method. Official methods of AOAC, UK, and Japan with HPLC-UV detection were evaluated for the analysis of canthaxanthin by comparison of linearity, resolution, selectivity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery, inter-laboratory tests, and uncertainty measurement. The calibration curves showed high linearity with an $R_2$ value of over 0.999 for canthaxanthin standard solutions in all three official methods. The official method of Japan exhibited the best results in terms of resolution and selectivity, including the lowest LOD and LOQ. The average coefficients of variation were calculated as less than five of three institutes with a precision value less than 1, accuracy near 100%, and recovery ratio between $100{\pm}10%$. The expanded uncertainty for canthaxanthin was estimated to be $39.5{\pm}5.29mg/kg$ (95% confidence level, k=2), and the uncertainty of measurement was 13.4%. In this study, official methods of canthaxanthin were compared and the validities verified. The results will be further applied to establish an authorized analytical method for canthaxanthin in Korea.

• Korean Journal of Remote Sensing
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• v.26 no.2
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• pp.239-249
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• 2010

The Geostationary Ocean Color Imager (GOCI) data-processing system (GDPS), which is a software system for satellite data processing and analysis of the first geostationary ocean color observation satellite, has been developed concurrently with the development of th satellite. The GDPS has functions to generate level 2 and 3 oceanographic analytical data, from level 1B data that comprise the total radiance information, by programming a specialized atmospheric algorithm and oceanic analytical algorithms to the software module. The GDPS will be a multiversion system not only as a standard Korea Ocean Satellite Center(KOSC) operational system, but also as a basic GOCI data-processing system for researchers and other users. Additionally, the GDPS will be used to make the GOCI images available for distribution by satellite network, to calculate the lookup table for radiometric calibration coefficients, to divide/mosaic several region images, to analyze time-series satellite data. the developed GDPS system has satisfied the user requirement to complete data production within 30 minutes. This system is expected to be able to be an excellent tool for monitoring both long-term and short-term changes of ocean environmental characteristics.

• Korean Journal of Food Science and Technology
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• v.51 no.5
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• pp.420-424
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• 2019

An HPLC analysis method was developed and standardized for the detection of dieckol as a functional food ingredient in Ecklonia cava extracts. HPLC was performed using a Capcell Pak C18 column ($250{\times}4.6mm$, $5{\mu}m$) with a gradient elution of water and acetonitrile, both containing 0.1% (v/v) trifluoroacetic acid, at a flow rate of 1.0 mL/min at $25^{\circ}C$. The eluate was detected at 230 nm. For validation, the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ) of dieckol were measured. The calibration curve for the detection of dieckol had high linearity ($R^2=0.9994$), with LOD and LOQ values of 0.38 and $1.16{\mu}g/mL$, respectively. Recovery of the quantified compound ranged from 99.61 to 100.71%. The relative standard deviation values of the intra-day and inter-day precisions were less than 1.7 and 1.25%, respectively. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of dieckol in Ecklonia cava extracts.

• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.52-57
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• 2019

Method development and validation of decursin for the standardization of Angelica gigas Nakai as a functional ingredient and health food were accomplished. The quantitative determination method of decursin as a marker compound of aerial parts of Angelica gigas Nakai extract (AAGE) was optimized by HPLC analysis using a C18 column ($3{\times}150mm$, $3{\mu}m$) with 0.1% TFA in water and acetonitrile as the mobile phase at a flow rate of 0.5 mL/min and detection wavelength of 330 nm. The HPLC/PDA method was applied successfully to quantification of the marker compound in AAGE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation ($R^2$) of 0.9994 and the limit of detection and limit of quantitation were $0.011{\mu}g/mL$ and $0.033{\mu}g/mL$, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 1.10% and 1.13%, respectively. Recovery of decursin at 0.5, 1, 5 and $10{\mu}g/mL$ were 92.38 ~ 104.11%. These results suggest that the developed HPLC method is very useful for the determination of marker compound in AAGE to develop a health functional material.

• Korean Journal of Plant Resources
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• v.34 no.2
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• pp.177-185
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• 2021

The purpose of this study was to compare the chemical properties of the bokbunja distributed in Korea with the content of the bioactive substance ellagic acid. The bokbunja was Rubus coreanus group and Rubus occidentalis group were compared, domestic bokbunja and import bokbunja were compared. In bokbunja, free sugar was 30.89 ± 0.7 mg/g of Rubus coreanus and 29.05 ± 0.87 mg/g of Rubus occidentalis. and 27.28 ± 7.4 mg/g of domestic bokbunja and 21.58 ± 6.73 mg/g of import bokbunja. The free amino acids was 4.50 ± 0.08 mg/g of Rubus coreanus and 5.05 ± 0.08 mg/g of Rubus occidentalis. and 4.13 ± 1.09 mg/g of domestic bokbunja and 3.75 ± 0.31 mg/g of import bokbunja. Validation of the ellagic acid method was confirmed by comparing the retention time and spectrum of the standard and extract using HPLC. The calibration curve (R²) showed linearity of 0.9999. As a result of analyzing the ellagic acid content of each extraction solvent, DMSO and methanol mixture extracts were high, and Rubus coreanus was 2.56 mg/g and Rubus occidentalis was 3.16 mg/g, which was not significantly different (p < 0.05) In addition, the ellagic acid content of domestic bokbunja and import bokbunja was 2.83 mg/g and 2.99 mg/g, which was not significantly different (p < 0.05).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.446-452
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• 2021

As a higher standard for food consumption is required, the consumption of chicken meat that can satisfy the subdivided food preferences is increasing. In March 2003, the quality criteria for chicken carcasses notified by the Livestock Quality Assessment Service suggested quality grades according to fecal contamination and the size and weight of blood and bruises. On the other hand, it is too difficult for human inspection to qualify mass products, which is key to maintaining consistency for grading thousands of chicken carcasses. This paper proposed the computer vision algorithm as a non-destructive inspection, which can identify chicken carcass parts according to the detailed standards. To inspect the chicken carcasses conveyed at high speed, the image calibration was involved in providing robustness to the side effect of external lighting interference. The separation between chicken and background was achieved by a series of image processing, such as binarization based on Expectation Maximization, Erosion, and Labeling. In terms of shape analysis of chicken carcasses, the features are presented to reveal geometric information. After applying the algorithm to 78 chicken carcass samples, the algorithm was effective in segmenting chicken carcass against a background and analyzing its geometric features.

• Korean Journal of Remote Sensing
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• v.36 no.6_2
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• pp.1615-1627
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• 2020

The purpose of this study is to compare and analyze the performance of KOMPSAT-3 Digital Surface Model (DSM) made in overseas testbed area. To that end, we collected the KOMPSAT-3 in-track stereo image taken in San Francisco, the U.S. The stereo geometry elements (B/H, converse angle, etc.) of the stereo image taken were all found to be in the stable range. By applying precise sensor modeling using Ground Control Point (GCP) and DSM automatic generation technique, DSM with 1 m resolution was produced. Reference materials for evaluation and calibration are ground points with accuracy within 0.01 m from Compass Data Inc., 1 m resolution Elevation 1-DSM produced by Airbus. The precision sensor modeling accuracy of KOMPSAT-3 was within 0.5 m (RMSE) in horizontal and vertical directions. When the difference map was written between the generated DSM and the reference DSM, the mean and standard deviation were 0.61 m and 5.25 m respectively, but in some areas, they showed a large difference of more than 100 m. These areas appeared mainly in closed areas where high-rise buildings were concentrated. If KOMPSAT-3 tri-stereo images are used and various post-processing techniques are developed, it will be possible to produce DSM with more improved quality.

• Journal of Korea Water Resources Association
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• v.55 no.5
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• pp.321-331
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• 2022

In this study, we tried to present a method for calculating the amount of regression using a watershed modeling method that can simulate the hydrological mechanism of water balance analysis and agricultural water based on watershed unit. Using the soil water assessment tool (SWAT), a watershed water balance analysis was conducted considering the simulation of paddy fields for the Manbongcheon Standard Basin (97.34 km²), which is a representative agricultural area of the Yeongsan river basin. Before evaluating return flow, the SWAT was calibrated and validated using the daily streamflow observation data at Naju streamflow gauge station (NJ). The coefficient of determination (R²), Nash-Sutcliffe Efficiency (NSE), Root-Mean-Square Error (RMSE) of NJ were 0.73, 0.70, 0.64 mm/day. Based on the calibration results for three years (2015-2017), the quick return flow and the return rate compared to the water supply amount for the irrigation period (April 1 to September 30) were calculated, and the average return flow rate was 53.4%. The proposed method of this study may be used as foundation data to optimal agricultural water supply plan for rational watershed management.