• Title/Summary/Keyword: Cadmium ion

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Microbiological Characteristics of Heavy Metal Ion-Tolerant Microorganisms. (중금속 내성균주의 미생물학적 성질)

  • 유대식
    • Microbiology and Biotechnology Letters
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    • v.7 no.4
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    • pp.183-190
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    • 1979
  • Cadmium ion-tolerant microorganisms were isolated from the sludge and soil of a cadmium ion-polluted area, a zinc mineralized area, in Kyung Sang Pook Do, Korea. A strain, C-7, which showed tile highest tolerance to cadmium ion was selected by screening from 18 cadmium tolerant microorganisms. By the taxonomical characteristics of this strain, it was identified as a variant of Erwinia sp.. The strain grew in a medium cadmium ion up to a concentration of 2, 800 $\mu\textrm{g}$/ml and the maximum intercellular accumulation of Cd$^{2+}$ was measured to be 28.60 mg/g dried cells (57.2%) during incubation in medium containing 50 $\mu\textrm{g}$/ml under aerobic condition at 28$^{\circ}C$ for 24 hour.r.

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Characterzation of a Cadmium-ion Tolerant Strain of Hansenula anomala (카드뮴 내성 Hansenula anomala 균주의 특성)

  • 유대식;송형익;정기택
    • Korean Journal of Microbiology
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    • v.24 no.1
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    • pp.57-61
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    • 1986
  • In order to remove of cadmium from waste water an identification of a cadmium-ion tolerant yeast B-7 isolated from the sludge of zinc mining district was studied. By the taxonomecal characteristics of strain B-7 it was identified as Hansenula anomala B-7 or similar strain. The cadmium-ion tolerance of the strain B-7 was determined as $2,700{\mu}g/ml$ of cadmium-ion by density gradient agar plate method. The strain B-7 grew well in an aqueous medium containing $1,000{\mu}g/ml$ of cadmium-ion.

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Evaluating Cadmium Ion Removal in Aqueous Solutions and Cytotoxicity Evaluation of Carbon, Synthesized Layered Double Hydroxide, and Multi-wall Carbon Nanotube (활성탄, 합성 층상이중층 수산화물, 카본나노튜브를 이용한 수용액상의 카드뮴의 제거와 흡착제 독성 연구)

  • Kim, Tae-Gyung;Park, Bog-Soung;Jung, Yong-Jun
    • Journal of Korean Society on Water Environment
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    • v.33 no.2
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    • pp.211-218
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    • 2017
  • This experiment was carried out with the purpose of testing cadmium adsorption abilities of multi wall carbon nanotube (MWCNT), activated carbon, and synthesized layered double hydroxide (LDH). In the acidic condition, only MWCNT was effective for removing cadmium ion in the aqueous phase while other adsorbents rarely removed it. The MWCNT and cadmium ion adsorption reactions followed pseudo-first order kinetic. When the initial pH value was neutral (pH = 7), cadmium ion was rapidly removed by MWCNT and activated carbon in 4 hr (100% and 99.2%, respectively). Increasing adsorbent dosages affects the pH evolution and cadmium ion removal (0 to 99%). Cytotoxicity test showed that both MWCNT and LDH has low cytotoxic effects on three kind of human cells (A549, HS-294t, and HT-29).

Effect of Metallothionein on the Lipid Peroxidation and Aldehyde Oxidase Activity (Metallothionein이 지질과산화반응과 Aldehyde Oxidase활성에 미치는 영향)

  • Huh, Keun;Shin, Uk-Seob;Park, Jong-Min
    • Toxicological Research
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    • v.11 no.1
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    • pp.43-49
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    • 1995
  • The effects of metallothionein and cadmium ion on the hepatic aldehyde oxidase activity and brain lipid peroxidation were tested in vitro. The content of brain lipid peroxide at the condition of normal or $300{\mu}M$ Fe(II)-induced was remarkably reduced by the addition of metallothionein in the incubation mixture. The induced content of lipid peroxide by cadmium $(30{\mu}g/ml)$ ion was reduced by metallothionein $(100{\mu}g/ml)$. The activity of aldehyde oxidase was not affected by metallothionein, but cadmium ion $(8.38{\mu}g/ml)$ increased the activity of aldehyde oxidase about 80% compared to the control. The cadmium-induced activity of aldehyde oxidase was restored to the control level by metallothionein or penicillamine.

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Isolation of Cadmium Ion-resistant Bacteria and Resitance to Various Heavy Metals (카드봄 내성균의 분리와 각종 중금속에 대한 저항성의 연구)

  • Yeeh, Yeehn;Lee, Jong-Kun
    • Korean Journal of Microbiology
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    • v.17 no.2
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    • pp.49-57
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    • 1979
  • Cadimium ion-resistant microorganism was isolated from the sludge of wastewater. The physiological, morphological and other cultural data showed that this strain belonged to Citrobacter freudii. A clearcut distinction of growth among nutrient broth, typtic soy broth and synthetic medium was demonstrated. The resistant cells showed only slight mutagenic action. During the growth of bacterial population in resting state, the organisms reduced the initial level of resistance to cadmium ions when they were not kept in contact with cadmium ions in bacteral multiplication. And cadmium ion-resistant and cadmium ion-sensitive strain were found to show equal, lower or higher sensitivity to other heave metals.

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The Effect of Some Binary Additive Systems in the Electrodeposition of Cadmium (카드뮴 전해석출에서의 이성분첨가물계의 효과)

  • Lee, Kyung Ho
    • Analytical Science and Technology
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    • v.9 no.2
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    • pp.161-167
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    • 1996
  • An investigation was made of possible ways in which one could control the relative rates of cadmium deposition and hydrogen evolution by binary additive systems. Benzyl alcohol was employed as an additives due to its ability to form a hydrophobic film which inhibit the electroreduction of water to form hydrogen. The second additive was chosen to make the cadmium(II) ion less hydrophilic and increase its ability to cross the hydrophobic benzyl alcohol film and be electrodeposited at the cathode. It was shown by voltammetric and current efficiency studies that ion pairing and complexing additives could be used to accelerate the reduction of cadmium in the presence of the benzyl alcohol film. It was also shown that the benzyl alcohol film lowered the dielectric constant of the solution near the electrode enough to obtain ion pairing between the sodium ion and the negative chloride complex of cadmium and accelerate the reduction of the cadmium. This acceleration did not occur in the sulfate solution in the absence of chloride since cadmium(II) is primarily present as a positive aquo complex and ion pairing, if it occured, would not accelerate but would hinder reduction of cadmium.

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Mechanism of Cadmium Accumulation into the Cell of Cadmium-Ion Tolerant Yeast (카드뮴 내성 효모의 세포내 카드뮴 축적 기작)

  • 유대식;송형익;정기택
    • Microbiology and Biotechnology Letters
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    • v.18 no.3
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    • pp.233-238
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    • 1990
  • The mechanism of intracellular accumulation of cadmium in a cadmium-ion tolerant yeast, Hansenula ammala B-7, which is an extreme cadmium tolerant strain and has the ability to take up a large amount of cadmium was investigated. The amounts of cadmium taken up by the scalded yeast cells were 2 to 3 times more than the value of the living cells. The living Hansenula anomala B-7 cells adsorbed 74% of cadmium taken up onto the other layer of the cells and 26% of it accumulated inside the cells. But the scalded cells adsorbed 98.3% of cadmium taken up and accumulated 1.7% of it inside the cells. A cadmium uptake and its accumulation were accelerated up to 162.3% and 275.4% by Triton X-100 in the living cells, respectively. Whereas in the scalded cell cadmium uptake was not affected by Triton X-100. Furthermore the cadmium uptake and its accumulation were strongly inhibited by metabolic inhibitors like 2,4-dinitrophenol, sodium azide and potassium cyanide in the living cells, but in the scalded cells cadmium uptake was not affected by metabolic inhibitors. These results suggested that the intracellular accumulation of cadmium by the cadmium-tolerant Hansenula anomala B-7 cells was apparently dependent of biological activity, and also gave evidence of the existance of energy-dependent system.

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카드뮴이 카드뮴 내성 효모세포내의 효소 활성에 미치는 영향

  • 유대식;박은규;박정문
    • Microbiology and Biotechnology Letters
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    • v.24 no.3
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    • pp.268-273
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    • 1996
  • An extremely cadmium tolerant yeast, Hansenula anomala B-7 used to determine the modification of the intracellular enzyme activities by cadmium ion. The activities of alcohol dehydrogenase, phosphofructokinase, and cytidine deaminase were decreased up to 90%, 40%, and 86% compa- red with the control by 1 mM cadmium nitrate respectively, but the activities of malate dehydrogenase, 6- phosphogluconate dehydrogenase, cytochrome c oxidase, and alkaline phosphatase were increased up to 440%, 136%, 260% and 155% compared with the control by 1 mM cadmium nitrate respectively. These results show that the activities of the enzymes participating in Embden-Mayerhof pathway (e.g. anaerobic metabolism) were reduced by cadmium, but those involved in hexose monophosphate pathway and tricarboxylic acid cycle (e.g. aerobic metabolism) were stimulated in contrast. It has been suggested that the diminished activity of cytidine deaminase in pyrimidine nucleotide dissimilation occured due to the inhibited nucleotide dissimilation by cadmium ion; the enhanced activity of cytochrome c oxidase was specifically required in order to oxidize a raised amount of NADH and NADPH due to the increased aerobic metabolism.

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Removal of Cadmium Ion (Cd2+) by Pseudomonas aeruginosa Immobilized in Ca-Alginate Gel Beads in Packed-Bed Column Reactor (충전층 반응기내에서 고정된 Pseudomonas aeruginosa에 의한 Cd2+의 제거)

  • Choi, Kwang Soo;Kim, Chul Kyung
    • Clean Technology
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    • v.8 no.4
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    • pp.217-222
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    • 2002
  • The effects of initial cadmium ion concentrations (50, 100, 200, 300ppm), and feeding velocities (30, 45, 60mL/hr) on the removal ratio of cadmium ion by Pseudomonas aeruginosa ATCC 27853 immobilized in Ca-alginate gel beads in a packed-bed column reactor were investigated at operating temperature $37^{\circ}C$. The removal ratio of cadmium ion with variable initial concentration was decreased in the following order : 50ppm > 100ppm > 200ppm > 300ppm. The optimum removal conditions of cadmium ion by Pseudomonas aeruginosa ATCC 27853 were initial concentration 50ppm, feeding velocity 30mL/hr.

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Morphological Changes of Hansenula anomala B-7 by Cadmium Ion (카드뮴이온에 의한 Hansenula anomala B-7의 형태 변이)

  • 송형익;유대식
    • Korean Journal of Microbiology
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    • v.29 no.6
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    • pp.397-401
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    • 1991
  • Yeast-form cells of cadmium ion-tolerant Hansenula anomala B-7 were changed to mycelial cells in medium containing more than $400\mu$g/ml of cadmium. Moreover, the mycelial cells were exchanged into clumped cells in a medium containing more than $1,000\mu$g/ml of cadmium. Optimal conditions of mycelial cell formation were achieved in the presence of .$1,000\mu$g/ml of cadmium with shaking cultivation for 7 days. Glucan and mannan contents of the yeast cell wall frown with $1,000\mu$g/ml of cadmium decreased by 10% compared with those grown without cadmium. However, protein and lipid contents increased about 20% respectively. By cadmium, no significant findings in specific amino acid contents were discovered.

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