• 생물환경조절학회지
• /
• 제14권4호
• /
• pp.269-274
• /
• 2005

실파용으로 적정 양액농도를 구명하고자 수경재배용으로 선발한 '금장외대파'와 '토쿄구로파' 품종을 공시하여 담액식으로 하여 시험을 수행하였다. 양액종류 시험을 통해 선발된 야마자키 처방의 싹파 전용 양액$(NO_3^--N\;9.0,\;NH_4^+-N\;3.0,\;PO_4^{3-}-P\;6.0,\;K^+7.0,\;Ca^{2+}\;2.0,\;Mg^{2+}\;2.0,\;and\;SO_4^{2-}-S\;4.4me{\cdot}L^{-1})$을 이용하여 EC 0.6, 1.2, 1.8, $2.4dS{\cdot}m^{-1}$의 4수준으로 처리한 결과 초장, 구당 생체중과 건물중에 있어서 두 품종 모두 EC 1.2, 1.8, 2.4, $0.6dS{\cdot}m^{-1}$ 순으로 양호하였다. 최대 생체중을 위한 남장외대파와 '토쿄구로파'의 2차 회귀곡선식은 $y=-42.091x^2+171.79x+11.047 (R^2=0.8946,\; R=0.9458^*)$와 y=-50.069x2+157.58x+15.414$(R^2=0.9343,\;R=0.9692^{**})$ 이었고, 이에 따른 적정 양액농도는 각각 $1.68dS{\cdot}m^{-1}$과 $1.57dS{\cdot}m^{-1}$수준이었다. 따라서 실파 수경재배시 생육 초기에는 양액을 $1.2dS{\cdot}m^{-1}$수준의 저농도로 유지하고 정식 후 30일경인 생육 중기 이후에는 $1.6\~1.7dS{\cdot}m^{-1}$수준의 농도로 유지하는 것이 효율적이라 여겨진다.

• 한국환경보건학회지
• /
• 제23권4호
• /
• pp.133-138
• /
• 1997

The technology of removing phosphorous, considered as one of the most important control nutrients causing eutrophication in various water bodies, have been investigated by many researchers. Recently, phosphorous crystallization process is emerging as a new technology for phosphorous removal. In this study, waste oyster shells which can be easily obtained from the ocean, were used as a seed crystal, and their effects of several physical/chemical factors on the phosphorous removal efficiencies were examined by batch tests. Ca$^{2+}$ and pH affected phosphorous crystallization process using waste oyster shells. As alkalinity of wastewater increased, phosphorous removal efficiencies gradually decreased. Phosphorous removal efficiencies were increased, as specific area and contact efficiency per unit area of waste oyster shells were increased. In case of high temperature, phosphorous crystallization process was rapidly advanced and phosphorous removal efficiencies were increased. Dependig on X-ray diffraction analysis, it was showed that generation materials extracted from the surface of waste oyster shells with short reaction time were dominated by $CaHPO_4\cdot 2H_2O$, but progressed to $Ca_5(OH)(PO_4)_3$. The SEM observation reveals that the evident variations were hardly seen, but particle sizes of waste oyster shells were relatively bigger and showed forms of smaller plate than before.

• Journal of Microbiology and Biotechnology
• /
• 제25권4호
• /
• pp.496-502
• /
• 2015

Polyketide secondary metabolites share common precursor pools, acyl-CoA. Thus, the effects of engineering strategies for heterologous and native secondary metabolite production are often determined by the measurement of pikromycin in Streptomyces venezuelae. It is hard to compare the effectiveness of engineering targets among published data owing to the different pikromycin production media used from one study to the other. To determine the most important nutritional factor and establish optimal culture conditions, medium optimization of pikromycin from Streptomyces venezuelae ATCC 15439 was studied with a statistical method, Plackett-Burman design. Nine variables (glucose, sucrose, peptone, (NH₄)₂SO₄, K₂HPO₄, KH₂PO₄, NaCl, MgSO₄·7H₂O, and CaCO₃) were analyzed for their effects on a response, pikromycin. Glucose, K₂HPO₄, and CaCO₃ were determined to be the most significant factors. The path of the steepest ascent and response surface methodology about the three selected components were performed to study interactions among the three factors, and the fine-tune concentrations for maximized product yields. The significant variables and optimal concentrations were 139 g/1 sucrose, 5.29 g/l K₂HPO₄, and 0.081 g/l CaCO₃, with the maximal pikromycin yield of 35.5 mg/l. Increases of the antibiotics production by 1.45-fold, 1.3-fold, and 1.98-fold, compared with unoptimized medium and two other pikromycin production media SCM and SGGP, respectively, were achieved.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제5권4호
• /
• pp.234-241
• /
• 2000

The Saccharomyces cerevisiae PMR1 gene encodes a Ca2+-ATPase localized in the Golgi. We have investigated the effects of PMR1 disruption in S. cerevisiae on the glycosylation and secretion of three heterologous glycoproteins, human ${\alpha}$1-antitrypsin (${\alpha}$1-AT), human antithrombin III (ATHIII), and Aspergillus niger glucose oxidase (GOD). The pmr1 null mutant strain secreted larger amounts of ATHIII and GOD proteins per a unit cell mass than the wild type strain. Despite a lower growth rate of the pmr1 mutant, two-fold higher level of human ATHIII was detected in the culture supernatant from the pmr1 mutant compared to that of the wild-type strain. The pmr1 mutant strain secreted ${\alpha}$1-AT and the GOD proteins mostly as core-glycosylated forms, in contrast to the hyperglycosylated proteins secreted in the wild-type strain. Furthermore, the core-glycosylated forms secreted in the pmr1 mutant migrated slightly faster on SDS-PAGE than those secreted in the mnn9 deletion mutant and the wild type strains. Analysis of the recombinant GOD with anti-${\alpha}$1,3-mannose antibody revealed that GOD secreted in the pmr1 mutant did not have terminal ${\alpha}$1,3-linked mannose unlike those secreted in the mnn9 mutant and the wild type strains. The present results indicate that the pmr1 mutant, with the super-secretion phenotype, is useful as a host system to produce recombinant glycoproteins lacking high-mannose outer chains.

• 한국표면공학회:학술대회논문집
• /
• 한국표면공학회 2017년도 춘계학술대회 논문집
• /
• pp.155-155
• /
• 2017

Surface modifications are commonly utilized to adjust the properties of the titanium and its alloy surface to the specific needs of the medical applications, but there are disadvantages such as poor osteoconductive properties and low adhesion of bone cell to implant surface. In order to improve these disadvantages, changes in surface properties have an important effect on osseointegration during implantation. In this paper we applied new technological method for improving a unique surface modification using the characteristic of an electrolytic Solution. Thus, in the electrolyte containing NaF in Na2SO4, TiO2 nanoporous was uniformly formed, and HAp nanoparticles were electrodeposited around the TiO2 nanopores, but in the electrolyte containing NH4F in (NH4)H2PO4, the coarse protrusions including HAp nano particles were regularly deposited onto the TiO2 barrier layer. The surface characteristics and the distributed elements and have been investigated by EDS analysis, and ultra-fine structure of surface are carried out using FE-SEM. To investigate the behavior of the anion, the analysis of chemical states was performed by XPS, and the narrow spectrums for Ti2P, Ca 2p, and P 2p seems to be almost similar depending on the characteristics of the electrolyte solution respectively. In addition, Ca 2p spectrum could be resolved into two peaks for Ca 2p3/2 and 2p1/2 at 347.4 and 351.3 eV, which are related to hydroxyapatite. And, the P peak can also be deconvoluted into two peaks for P1/2 and P3/2 levels with binding energy 134.2 and 133.4 eV, respectively. From the result of soaking test, the apatite morphologys were well-formed onto the modified surface according to the different conditions.

• The Plant Pathology Journal
• /
• 제21권3호
• /
• pp.270-274
• /
• 2005

The aim of this study was to determine the effect of milk as one of the environmental friendly materials that substitute chemical fungicides for control powdery mildew (Sphaerotheca aphanis) of strawberries $(Fragaria{\times}ananassa\;Duch.)$. 'Maehyang' and 'Akihime' varieties planted in greenhouses were evaluated for the control of powdery mildew. Applications of $5\%,\;10\%\;and\;20\%$ milk had much better effects on controlling powdery mildew. In particular, $10\%$ milk showed a higher efficacy than other concentrations applied onto straw­berry in greenhouse experiments. Foliar spray application of $10\%$ milk was effective for powdery mildew, whereas drench application was not. Also, foliar spray of $10\%$ milk was able to accelerate more firmness and calcium contents of strawberry fruits than the non-treated. The $10\%$ milk applied with fertilizer salts $(Ca(H_2PO_4)_2-H_2O\;and KH_2PO_4)$ showed there was a similar efficacy to $10\%$ milk alone in greenhouse experiments. White crystals and cracks on strawberry fruits appeared by $20\%$ milk. This result indicated that $10\%$ milk was a useful substitute for fungicides to control powdery mildew of strawberry.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2001년도 The 8th International Symposium
• /
• pp.74-82
• /
• 2001

It was reported in our Previous paper that the fermented products from Gloeostereum incarnatum strongly inhibit the growth of six kinds of bacteria in human bodies. In this paper the appropriated conditions of immersing culture for the strain 8 903 of Gloeostereum incarnatum was analysed. And the output of the hypha and fermentative product was determined or compared, The prelimenaryresults showed that the appropriated conditions for the growth of Gloeostereum incarnatum are: (1)culture medium:glucose 3%; protein peoptne 0.2%; soybeancake power 1%, yeast power 0.3%; KH2PO40.05%; MgSO4 0.03%; CaCO3 0.01%; vitamin Bl 0.001%; befor sterilization pH Value of six should be maintained; (2) temperature; 27$^{\circ}C$～28$^{\circ}C$; (3) time; about 200 hours; (4) ventilation; (30%～50%)/min. The sigh of the end culture we: pH coming down about 4: remnant glucoses less 1%, amino nitrogens about 20;, time about eight days. In the aforementioned conditions, the output of fermentative product achieve to 2.5 ～3g/L.

• 한국미생물·생명공학회지
• /
• 제21권3호
• /
• pp.256-262
• /
• 1993

Nine novel cyclodextrin glycosytransferase-producing bacteria were isolated from soil in a low acidic pH (3-4) medium at high temperature (45-60C). The isolated acidophilic bacteria were identified as Bacillus acidocaldarius. Highest yield of enzyme was obtained by using the following medium: 4% raw potato, 1% peptone, 0.1% yeast extract, 0.02% (NH4)2SO4, 0.05% MgSO4, 0.02% CaCl2, 0.3% KH2PO4. The crude enzyme showed a very broad pH-activity curve and had two optium pH ranges at 30 and 5.0-6.0. The crude enzyme was most active at 90C.

• 한국식품과학회지
• /
• 제32권1호
• /
• pp.154-160
• /
• 2000

한국 전통 메주로부터 분리 동정한 Bacillus subtilis PCA20-3이 생산하는 protease의 생산조건과 특성을 조사하였다. Protease의 생산 최적조건을 0.2% soytone, 2% starch, 0.1% $(NH_4)_2SO_4,\;0.2%\;CaCl_2,\;0.01%\;yeast\;extract,\;0.1%\;K_2HPO_4,\;0.1%\;KH_2PO_4$, pH 7.0, 30에서 20시간이었다. 효소의 최적작용 pH와 온도는 6.0-11.0, $55^{\circ}C$였고 pH $6.0{\sim}11.0$의 범위와 $50^{\circ}C$이하에서 안정하였다. 금속이온중 $Fe^{(2+)}$와 $Cu^{(2+)}$에 의해 효소활성이 저해되었다. 2mM의 phenymethanesulfonyl fluoride에 의해 89.2%의 활성이 저하되어, 활성 serine을 가진 serine protease임이 시사되었다. 조효소액의 Km값은 $5.0{\times}10^{(-4)}M,\;V_(max)$값은 $100\;{\mu}g/min$이었으며 bovine serum albumin, isolated soybean protein 보다 casein에 대해 초기 가수분해력이 높은 것으로 나타났다.

• Journal of Ceramic Processing Research
• /
• 제20권6호
• /
• pp.655-659
• /
• 2019

Calcium phosphates such as HA (hydroxyapatite), β-TCP (tricalcium phosphate) and biphasic HA/β-TCP, were synthesized by wet chemical precipitation in aqueous solution combined with ball milling process. Nanosize powders of the calcium phosphates were synthesized using Ca(OH)2 and H3PO4. The effects of initial precursor Ca/P ratio (1.30, 1.50 and 1.67), ball milling process and post heat-treatment on the phase evolution behavior of the powders were investigated. The phase of resulting powder was controllable by adjusting the initial Ca/P ratio. HA was the only phase for as-prepared powders in both cases of Ca/P ratios of 1.50 and 1.67. The single HA phase without any noticeable second phase was obtained for the initial Ca/P ratio of 1.67 in the overall heat-treatment range. Pure β-TCP and biphasic calcium phosphate (HA/β-TCP) were synthesized from precursor solutions having Ca/P molar ratios of 1.30 and 1.50, respectively, after having been heat-treated above 700 ℃. The β-TCP phase has appeared on the pre-existing DCPD (dicalcium phosphate dihydrate) and/or HA phase. Dense ceramics having translucency were obtained at a considerably lower sintering temperature. The modified process offered a fast, convenient and economical route for the synthesis of calcium phosphates.