• Title/Summary/Keyword: CULTURE

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Effect of Ionic Copper Toxicity on the Growth of Green Alga, Selenastrum capricornutum

  • Kim, Mi-Kyung;Ralph E. H. Smith
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.211-216
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    • 2001
  • The growth of Selenastrum capricornutum in culture was investigated as a function of ionic copper concentrations to verify the toxicity and physiological variation. In a $Cu^{++}$ excess culture (E: $Cu^{++}$ of $130{\mu}g/l$), the growth rate (K)(0.32) was lower than that of the control culture (C: $Cu^{++}$ of $0.065{\mu}g/l$) (0.61) after 8 days and the exponential growth rate ($R_E$) was also found to be lower in culture E (1.1) than culture C (2.9). On the contrary, the K of S. capricornutum in trace $Cu^{++}$ culture ($T_1$ and $T_2$, 0.72) after 6 days tended to be more increased than culture C (0.68). From 8 to 14 days of culture, the amounts of chlorophyllls a and b were increased in culture C (chlorophyll a, $106->126{\mu}g/g$ dry wt; chlorophyll b, $158->208{\mu}g/g$ dry wt; chlorophyll b were decreased in culture E (chlorophyll a, $309->235{\mu}g/g$ dry wt; chlorophyll b, $405->352{\mu}g/g$ dry wt). The amounts of chlorophylls in ionic copper trace culture ($T_1$ and $T_2$) [(chl a/b) of $T_1$: $384/620\;{\mu}g/g$ dry wt; (chl a/b) of $T^2$:$320/467{\mu}g/g$ dry wt] were increased more than the culture C($260/387{\mu}g/g$ dry wt). Howeveer, when photosynthetic rates were normalized to the dry weight of algae, the control culture continued to showed higher values than the treated culture ($T_1$). An appropriate amount of ionic copper ($T_1:\;26\;{\mu}g/l$) stimulated the grwoth of S. capricornutum than the ionic copper content of $13\;{\mu}g/l$ ($T_2$), while the excess amount of ionic copper ($130\;{\mu}g/l$) resulted in the highest toxicity to the growth of S. capricornutum.

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Evaluation of Nutrients during Rack and Bag Culture or Suspended Culture of Pacific Oyster Crassostrea gigas (부유망식과 수하식 양성방법에 따른 참굴(Crassostrea gigas)의 영양상태)

  • Choi, Yong-Jun;Tri, Nguyen Thanh;Lee, Jeong-Mee;Kang, Seok-Joong;Choi, Byeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.3
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    • pp.263-269
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    • 2017
  • The mineral contents, and free amino acid, and fatty acid composition of rack and bag or suspended cultured oysters Crassostrea gigas were compared. The moisture content did not change much from 82.8% to 86.3% in the different culture methods. The glycogen level was low 4.7% during rack and bag culture but high 7.2% during suspended culture. The sodium level was the highest during suspended culture, while copper and zinc level were highest during rack and bag culture. The taurine level was 1,385-1,375 mg/100 g in oyster cultured using the rack and bag method, but that in the market sample was lowest at 1,015.8 mg/100 g. Hydroxyproline, glutamic acid, proline, glycine, and alanine were detected at high levels in the experimental oysters. The polyunsaturated fatty acid content was similar among samples. The docosahexaenoic acid level was 14% with rack and bag culture and 12% with suspended culture, whereas the eicosapentaenoic acid level was 15% with rack and bag culture and 20% with suspended culture. The atherogenic index (AI), thrombogenicity index (TI), and hypocholesterolemic/ hypercholesterolemic (h/H) ratio were important factors in the nutritional evaluation. The AI and TI values were 0.5-0.2, and the h/H ratio were 2.0 and 2.4 with the rack and bag and suspended culture, respectively.

Growth of Axenic Rotifer Brachionus rotundiformis (무균 로티퍼 Brachionus rotundiformis의 증식)

  • Jung, Min-Min;Rho, Sum;Kim, Pil-Yun
    • Journal of Aquaculture
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    • v.11 no.1
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    • pp.91-97
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    • 1998
  • This paper introduces to a simple culture method and growth of axenic (bacteria-free) rotifer Brachionus rotundiformis for seed stock of rotifer mass culture. This rotifer axenic culture method is based on the washing and transferring with sterilized sea water and modified antibiotic mixture AM9 solution. Population growth (final density on day 16) of axenic cultured rotifer were maintained with a high density and stable growth compared with the control of non-axenic culture (general culture style) through the 3 times-rerunning experiments (trial 1, 2 and 3). But the egg carrying rates of amictic females were not different between the axenic-and non-axenic culture condition. Although, rotifer density was higher in axenic culture, the food (Nannochloropsis oculata) was still remained unutilized than that of the non-axenic culture in third trial culture. These results suggest the possible existence of harmful bacteria for the rotifer population growth in the trial 1 and 3 of non-axenic culture compared to the trial 2. This axenic rotifer culture method is valuable for seed stock of the stable rotifer mass cultures.

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Study on the development of Quantitative assessment indicator of safety culture for the construction site (건설현장 안전문화의 정량적 평가지표 개발에 관한 연구)

  • Jun, Heakyung;Kwon, Changhee
    • Journal of the Society of Disaster Information
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    • v.12 no.4
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    • pp.403-411
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    • 2016
  • The objectives of this study is to develop evaluation indicators for the quantitative evaluation of construction safety culture level in order to prevent accidents by evaluating the level of safety culture and each safety culture elements of the construction site and to present the areas that should be focused on improvements. In this study, it was presented assessment indicators of the construction safety culture by analyzing previous studies for safety culture, by categorizing items as an important element of safety culture hierarchically and by reflecting the opinion of the construction site professional personnels using AHP analysis methodology. The assessment indicators of the construction safety culture were structured the details of the leadership, systems, and personal characteristics and derived weighted value by the pairwise comparison to quantify the detail assessment indicators in order to assess the construction safety culture level. This study presents a safety culture assessment indicators for the construction site to suggest directions for improving the construction site safety culture and prevent the accidents of the construction site by derived via a safety culture assessment of construction site.

Mass Culture of Ultra-small Rotifer, Synchaeta kitina at the Exchange Rate of Culture Water and Initial Inoculation Density (환수율 및 접종밀도에 따른 초소형 rotifer, Synchaeta kitina의 대량배양)

  • Oh, Jeong-Soo;Park, Jin-Chul;Park, Heum-Gi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.4
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    • pp.354-359
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    • 2009
  • The productivity of ultra-small rotifer, Synchaeta kitina was investigated at the exchange rate of culture water (10, 20, 30, 40 and 50%) and initial inoculation densities (250, 600 and 900 inds. per mL) in semi-continuous culture. Also, the possibility of mass culture was investigated in a 100 L culture tank. Tetraselmis suecica was used as the feed for S. kitina in all experiments. The production of S. kitina increased with an increase in exchange rate of culture water. The highest production ($82.0{\times}10^5$ inds.) was achieved at 40% exchange rate of culture water. The production of S. kitina increased with an increase of initial inoculation density during the first week and the highest total production ($17.4{\times}10^6$ inds.) was achieved at 900 inds. per mL of initial inoculation density. However, on the second week, all treatments were not significantly different in total production (P>0.05). During the two weeks, total production of S. kitina at 900 inds. per mL of initial inoculation density was higher than at 600 inds. of initial inoculation density, but there was no significant difference (P>0.05). In the 100 L culture tank, density of S. kitina was kept from 516 to 890 inds. per mL and S. kitina was daily harvested $15.5{\times}10^6$ to $26.7{\times}10^6$ during the experimental period. The production cost for 100 million rotifers in semi-continuous culture was 63,656 won. The results from this study indicate that the optimal exchange rate of culture water and initial inoculation density for the semi-continuous culture of ultra-small rotifer, S. kitina are 40% and 600 inds. per mL, respectively.

The Effects of Police Organizational Culture & Organizational Commitment on Social Loafing (경찰공무원의 조직문화인식과 조직몰입이 사회적 태만에 미치는 영향)

  • Joo, Jae-Jin
    • The Journal of the Korea Contents Association
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    • v.16 no.1
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    • pp.438-444
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    • 2016
  • Previous findings on Social Loafing indicated that Social Loafing explained the organizational commitment, that the lower level of Social Loafing they had, the higher performance they would have, and that it is a useful index for effectiveness of the organization. This study explored the Police Organizational culture & Organizational Commitment on Social Loafing. The Police officers' Police Officer's Organizational culture & Organizational Commitment and Social Loafing has been measured, and then how Organizational culture & Organizational Commitment affects Police Officer's Social Loafing has been examined. The study results may be used as a theoretical basis to assert the need of Organizational culture & Organizational Commitment to decrease the Police Officer's Social Loafing. Independent variable is Police Officer's Social Loafing and Dependent variable is comprises 2 categories; Police Organizational culture(The group culture, The hierarchical culture, The developmental culture, The rational culture), & the Organizational Commitment(Affective Commitment, Continuance Commitment, Normative Commitment). The result of analysis showed that The group culture & Normative Commitment influenced in Social Loafing(positive), and The Rational culture, Affective Commitment, & Continuance Commitment in Social Loafing(negative).

A Case Study on the Costume Culture of the Head Family for Adolescent′s Spot Experience Studying (청소년의 종가 생활문화 현장학습프로그램 개발을 위한 종가 복식문화 사례 연구 -전남 함평 종가를 대상으로-)

  • 서리나;유명의
    • The Research Journal of the Costume Culture
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    • v.11 no.5
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    • pp.753-766
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    • 2003
  • The purpose of this study was to examine the cases of the family head's costume culture and to apply them to field experience education of adolescent's home economics subject. To examine the family head's costume culture, women in the family head in the Hampyeong area, Jeollanam-do were selected and interviewed. The focus of examining the family head's costume culture was on the life story of the family head, the reason to wear or possess such costume, and a motive of making. The findings of the head family's costume culture suggests that the head family of the Mos of Hampyeong had unique shroud making and custom. Also it was found that the eldest daughters-in-law of the Lees of Hampyeong have horizontally exchanged the information of the family head's costume with village women of the same family. They have initiated their costume culture directly through their daughters and it had the great influence on after-ages. The learning program for youth to experience the head family's costume culture consisted of four subjects:(l)Educating the family head's traditional living culture including etiquette training, (2)head family village walk, (3)experiencing the family head's clothes life including natural dyeing and sewing, and (4)a field trip of Hampyeong Local Life Culture Museum. For a model experience of the family head's costume life culture, three families with middle and high schoolers participated in 8- hour experience learning program. To examine the effect of learning program to experience the family head's costume culture, youth and their parents who participated in the program were asked open-ended questions, which included the content validity of experience learning, impressive experience, model experience level(difficulty), and expected effect after a model experience. As a result, their responses were found positive.

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A Study on the introduction & expansion Strategy of New Maritime Culture in Korea (우리나라 새로운 해양문화의 도입과 확장 전략에 관한 연구)

  • Kim, Hong-Seop
    • Journal of Korea Port Economic Association
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    • v.26 no.4
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    • pp.269-288
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    • 2010
  • 21 C is called the age of Culture and it is underlined in all the Areas of Human lifes. Among all activities, Culture oriented Thinking and activities and strategies through and for Culture establishing are emphasized. As Sea may appear as a image of longing and challenge for the unknown world, Port is understood as a link which connect Sea and Land. Sea, Island and Port are the basis of human lifes, economic working areas and communication spaces with nature. Sea and Port have been changed in accordance with human History as the space of Life, Work, Play and Imagination. The Maritime activities has been developed as the type of a Culture, including various diversities according to the time and space. This Paper suggested a definition of Maritime Culture and Theoretical review and groped introduction and strategies and countermeasures for expansion of systematic Maritime Culture. Maritime Culture not only enhance the quality of Citizen life but also contribute the progress of National economy increasing the quality of Culture, Turism and Environment and the expectation of it has been enlarged. It is needed to introduce and expand Maritime Culture systematically through theoretical framework suggested by this paper, and additional research may be necessary for the detailed solutions and countermeasures.

Influence of Nursing Organization Culture on Resilience in New Nurses (간호조직문화가 신규간호사의 회복탄력성에 미치는 영향)

  • Lee, Miyoung;Im, Sookbin;Byun, Eun Kyung;Chung, Eunja
    • Journal of Korean Clinical Nursing Research
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    • v.24 no.2
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    • pp.137-146
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    • 2018
  • Purpose: The purpose of this study was to identify the influence of nursing organization culture on resilience of new nurses. Methods: Participants were 1,033 new nurses with careers of less than 12 months and who were from 43 general hospitals located in Seoul, Incheon, other metropolitan cities and provinces in Korea. The data were analyzed using t-test, ANOVA, Pearson correlation coefficient and multiple regression with SPSS/WIN 24.0 program. Results: Relation-oriented culture was scored highest for perception of organizational culture by new nurses (3.35), followed by hierarchy-oriented culture (3.19), innovation-oriented culture (3.12), and task-oriented culture (2.73). The score for resilience of the new nurses was 3.49, and the first subcategories were interpersonal relationship ability 3.66, self-positivity 3.45, and self-control ability 3.36. Relation-oriented culture (${\beta}=.30$, p<.001) was the most significant factor influencing resilience of new nurses, followed by innovation-oriented culture (${\beta}=.14$, p=.001), hierarchy-oriented culture (${\beta}=.12$, p<.001), task-oriented culture (${\beta}=-.10$, p=.003) in that order ($R^2(adj. R^2)=.16$, F=47.04, p<.001). Conclusion: Findings show that for improved resilience in new nurses it is necessary to develop relation-oriented culture to provide a cooperative work environment(friendly interpersonal relation and dialogue) for new nurses and to encourage involvement of these members in the hospital culture.

A Study on the Conversation Textbooks with Chinese Culture: an Analysis of the Problems on Talking Culture and Comparison with Textbooks of Korea, Japan (문화 소재 중심의 중국어 회화교재에 대한 일고 - 『설한어(說漢語) 담문화(談文化)』의 문제점 분석과 극복방안으로서의 한·일 교재 검토)

  • Park, Chan-Wook
    • Cross-Cultural Studies
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    • v.40
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    • pp.133-158
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    • 2015
  • This study aims to analyse the conversation structure on Talking Culture("說漢語 淡文化") that is one of the conversation textbooks about Chinese culture and investigate Chinese textbooks published in Korea and Japan from a integration point of view among language, literature and culture for improving upon the problems on Talking Culture. For this purpose, this study, before analysing and investigating, considered the concept of language socialization on learning Chinese as a foreign language, and on the basis of it, analysed the conversation structure of Talking Culture. And then this study examined how we should organize the structures and contents when making conversations in Chinese textbook related with culture in compared with the Chinese textbooks published in Korea and Japan. In conclusion, this study argues that when composing a conversation textbook with culture, we not only need to pay attention not to have an inclination for conversation structure, but need to make use of the contents in Chinese literary and culture works for organizing conversations from the perspective of integration among language, literature and culture.