• Title/Summary/Keyword: CPC1

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Effects of Cervi Parvum Cornu on the Cell Cycle Regulation in Human Periodontal Ligament Cells (녹용이 치주인대세포의 세포주기조절에 미치는 영향)

  • You Seung Han;Choi Hee In;Kim Hyun A;Kim Yun Sang;Shin Hyung Shik;You Hyung Keun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.1
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    • pp.157-164
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    • 2003
  • Cervi Parvum Cornu(CPC) is that the young horn of deer family and has been traditionally used as a medicine in Eastern. The purpose of present study was to investigate the effects of CPC on cell cycle progression and its molecular mechanism in human periodontal ligament cells (HPOLC). In cell proliferation assay, 1 ng/ml, 10 ng/ml, 100 ng/ml, 1 ㎍/ml and 10 ㎍/ml of CPC were used, all treatment groups increased the cell growth. Maximal cell proliferation was observed in cells exposed to 100 ng/ml of CPC at 4 day, and 10 ng/ml and 100 ng/ml of CPC at 6 days. S phase was increased and G1 phase was decreased in the group treated with 100 ng/ml of CPC in cell cycle analysis. The protein levels of cyclin D1 were not changed, but the levels of cyclin E, cdk 2, cdk 4 and cdk 6 were increased. The protein levels of p21, pRb were decreased as compared to that of control group, but the levels of p53 was not changed in the cells both treated with CPC Md untreated. These results suggested that CPC increases the cell proliferation and cell cycle progression in HPDLC, which is linked to an increased cellular levels of cyclin E, cdk 2, cdk 4 and cdk 6, and decreased the levels of p53, p21.

Rheological Properties of Calcium Phosphate Cement Mixed with 2 Kinds of Setting Solution (2종의 경화액과 혼합된 calcium phosphate cement의 유변학적 성질에 관한 연구)

  • Chang, Seok-Woo;Kwon, Ho-Beom;Yoo, Hyun-Mi;Park, Dong-Sung;Oh, Tae-Seok;Bae, Kwang-Shik
    • Journal of Dental Rehabilitation and Applied Science
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    • v.24 no.3
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    • pp.311-316
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    • 2008
  • Calcium phosphate cement (CPC) has been used as bone substitute successfully due to good biocompatibility and osteoconductivity. One of the important mechanical characteristics of CPC is flowablility, which can be evaluated by measuring rheological parameters. However, there have been few studies that measured rheological properties of CPC. The purpose of this study was to evaluate the rheological properties of CPC paste mixed with 2 kinds of setting solutions, 2% hydroxyprophyl methylcellulose (HPMC) and 35% polyacrylic acid (PAA). The CPC used was dicalcium phosphate dihydrate (DCPD). Rheological properties of CPC paste were measured using rheometer. The statistical analysis was carried out with Mann-whitney test with Bonferronis collection. CPC with both setting solutions showed shear thinning behavior. CPC with 2% HPMC showed signigicantly higher complex viscosity than CPC with 35% PAA(p<0.05).

Applying Workflow Management System to CPC (CPC에서의 Workflow 응용)

  • 전희철
    • CDE review
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    • v.8 no.1
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    • pp.58-65
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    • 2002
  • Collaborative product commerce(CPC) involves many people working together with heterogeneous and distributed software applications. For such an environment, Workflow Management System(WFMS) can be useful for coordination of people, software agents and processes. It provides diverse services including automatic work muting, project management continuous process improvement and application integration. However, there are some limitations to apply WFMS to CPC environment due to inflexibility and lack of design support facilities. This paper identifies the problems and addresses possible approaches to overcome the difficulties.

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Study on the Micellization of CPC/Brij 35 Mixed Surfactant Systems in Water (순수 물에서 CPC/Brij 35 혼합계면활성제의 미셀화에 대한 연구)

  • Gil, Han-Nae;Lee, Byung-Hwan
    • Journal of the Korean Chemical Society
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    • v.53 no.2
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    • pp.118-124
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    • 2009
  • The values of critical micelle concentration (CMC) and counter ion binding constants (B) in a micellar state of CPC (1-hexadecylpyridinium chloride) with Brij 35 (polyoxyethylene(23) lauryl ether) in water were determined as a function of ${\alpha}_1$ (the overall mole fraction of CPC) by the use of electric conductivity method. Various thermodynamic parameters ($X_i,\;{\gamma}_i,\;C_i,\;a_{i}^{M},\;\beta,\;and\;{\Delta}H_{mix}$) were calculated and analyzed by means of the equations derived from the non-ideal mixed micellar model. And thermodynamic parameters (${\Delta}{G^o}_m,\;{\Delta}{H^o}_m,\;and\;{\Delta}{S^o}_m$) for the micellization of CPC/Brij 35 mixtures were also calculated from the temperature dependence of the CMC values. The values of ${\Delta}{G^o}_m$ are all negative, but the values of ${\Delta}{S^o}_m$ and ${\Delta}{H^o}_m$ are positive or negative, depending on the measured temperature and ${\alpha}_1$.

Isolation and Purification of Antibacterial Components in Cortex Phellodendri (황백나무로부터 항균성분의 분리 및 정제)

  • Kim, Jung-Bae;Shin, Woon-Seob;Kwon, Young-In;Bang, Byung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.26 no.3
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    • pp.547-552
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    • 2013
  • Cortex Phellodendri (CP) is derived from the dried bark of Phellodendron amurense. It has been widely used as a drug in traditional Korea medicine for treating diarrhea, jaundice, swelling pains in the knees and feet, urinary tract infections and infections of the body surface. In this study, preparative centrifugal partition chromatography (CPC) was successfully carried out to separate antibacterial compounds from a CP methanol extract. The optimum two-phase CPC solvent system was composed of n-butanol: acetic acid: water (4:1:5 v/v/v). The flow rate of the mobile phase was 3 $m{\ell}/min$ in ascending mode with rotation at 1,000 rpm. The CPC-separated fraction and purification procedures were carried out by preparatory HPLC. Palmatine weas identified by $^1H$, $^{13}C$-nuclear magnetic resonance and electrospray ionization-mass spectroscopy spectral data analysis.

Comparison of the Thermal Performance with Stationary and Tracking Evacuated CPC Collectors (고정형과 추적형 Evacuated CPC 집열기의 열성능 비교)

  • Yun, Seong-Eun;Kim, Yong;Seo, Tae-Beom
    • Journal of the Korean Solar Energy Society
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    • v.24 no.4
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    • pp.19-25
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    • 2004
  • A numerical study is performed to investigate the effect of sun tracking on the thermal performance of the evacuated compound parabolic concentrator (CPC) collectors. The evacuated CPC collectors consist of a two-layered glass tube, a copper tube and a reflector. The collector has a copper tube as an absorber and a reflector inside a glass tube. The water is used as a working fluid. The length and the diameter of the glass tube are 1,700mm and 70mm, respectively. The length and the diameter of the copper tube are 1,700mm and 25.4mm, respectively. Ray tracing analysis is carried out in order to compare absorbed heat fluxes on the absorber surface of the stationary and tracking collectors. The collected energy is calculated and compared with that on a fixed surface tilted at $35^{\circ}$ on the ground and facing south. The results indicate that the collected solar energy of the sun tracking system is significantly larger than that of a stationary collector. The sun tracking evacuated CPC collectors show a better performance with an increase in the thermal efficiency of up to 14% compared with an identical stationary collector.

Evaluation of Korean Version of the Beliefs about Papanicolau Test and Cervical Cancer in Unmarried University Students (한국판 자궁경부암과 Pap 검사 신념 도구의 타당도 신뢰도 평가: 미혼 여대생 대상)

  • Kim, Hae Won;Ko, Ga Yeon
    • Women's Health Nursing
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    • v.19 no.1
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    • pp.13-22
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    • 2013
  • Purpose: This study was conducted to evaluate Korean version of the beliefs about Papanicolau (Pap) test and cervical cancer [CPC-28] in unmarried university students. Methods: The Korean version of CPC-28 (K-CPC-28) was developed through forward-backward translation techniques. A reliability, confirmatory factor analysis and correlations coefficients were evaluated. Data were collected from 303 unmarried female students attending university using a questionnaire that included28 items of CPC, 5 items of susceptibility of cervicalcancer and 8 items of HPV knowledge. Results: K-CPC-28 had reliable internal consistency with Cronbach's ${\alpha}$=.74 of six subscales ranged from .66 to .80. Factor loadings of the 28 items of subscales ranged from .31 to .86. Six factors in this study explained 55% of the total variance. In convergent validity of the K-CPC-28, the subscales of K-CPC-28 were significantly correlated with susceptibility scale of cervical cancer and HPV knowledge scale. Conclusion: K-CPC-28has satisfactory construct validity and reliability. It seems to be an acceptable tool to assess the attitudes toward cervical cancer prevention and Pap smear in unmarried women. This tool would be also applicable to screen the risk group in cervical cancer prevention and to identify its association with actual Pap test or cervical cancer prevention behaviors.

Cyanobacterial Diversity Analysis Using cpcBA-Intergenic Spacer Region (cpcBA-Intergenic Spacer Region을 이용한 Cyanobacteria의 다양성 분석)

  • Choi Gang-Guk;Park Yong-Ha;Ahn Chi-Yong;Bae Myoung-Sook;Oh Hee-Mock
    • Korean Journal of Microbiology
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    • v.41 no.4
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    • pp.287-292
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    • 2005
  • The cyanobacterial diversity was analyzed by restriction fragment length polymorphism (RFLP) of PCR-amplified rpcBA-Intergenic Spacer (IGS) genes and cpcBA-IGS gene sequencing with a sample collected at Chuso-ri in Daechung Reservoir on March 15, 2005, The Shannon-Weiner diversity index was 0.65, indicating that the cyanobacterial community structure was simple. PCR-RFLP profiles obtained were Phormidium spp. (58 clones), Anabaena spp. (14 clones), Microcystis spp. (4 clones), Spirulina sp. (1 clone) and uncultured cyanobacteria (2 clones). The PCR-RFLP of cpcBA-IGS revealed that Phormidium spp. and Anabaena spp. dominated in the invested sample. As a consequence, it seems that the analysis of functional genes such as cpcBA-IGS can be used for the species identification and community analysis of cyanobacteria.

Clinical Experience of the Brushite Calcium Phosphate Cement for the Repair and Augmentation of Surgically Induced Cranial Defects Following the Pterional Craniotomy

  • Ji, Cheol;Ahn, Jae-Geun
    • Journal of Korean Neurosurgical Society
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    • v.47 no.3
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    • pp.180-184
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    • 2010
  • Objective : To prevent temporal depression after the pterional craniotomy, this study was designed to examine the safety and aesthetic efficacy of the brushite calcium phosphate cement (CPC) in the repair and augmentation of bone defects following the pterional craniotomy. Methods : The brushite CPC was used for the repair of surgically induced cranial defects, with or without augmentation, in 17 cases of pterional approach between March, 2005 and December, 2006. The average follow-up month was 20 with range of 12-36 months. In the first 5 cases, bone defects were repaired with only brushite CPC following the contour of the original bone. In the next 12 cases, bone defects were augmented with the brushite CPC rather than original bone contour. For a stability monitoring of the implanted brushite CPC, post-implantation evaluations including serial X-ray, repeated physical examination for aesthetic efficacy, and three-dimensional computed tomography (3D-CT) were taken 1 year after the implantation. Results : The brushite CPC paste provided precise and easy contouring in restoration of the bony defect site. No adverse effects such as infection or inflammation were noticed during the follow-up periods from all patients. 3D-CT was taken 1 year subsequent to implantation showed good preservation of the brushite CPC restoration material. In the cases of the augmentation group, aesthetic outcomes were superior compared to the simple repair group. Conclusion : The results of this clinical study indicate that the brushite CPC is a biocompatible alloplastic material, which is useful for prevention of temporal depression after pterional craniotomy. Additional study is required to determine the long-term stability and effectiveness of the brushite calcium phosphate cement for the replacement of bone.

Spectrophotometric Determination of Rare Earths by Ternary Complex Formation with MTB and Surfactant (글로방전 발광분광법에 의한 란탄족 원소의 정밀 분석. MTB와 계면활성제의 삼성분 착물 형성에 의한 희토류 원소의 분광광도법 정량에 관한 연구)

  • Cha Ki-Won;Yun Jeong-Sook;Kim Kyung-Hwan;Ha Young-Gu;Kim Ha-Suek
    • Journal of the Korean Chemical Society
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    • v.37 no.5
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    • pp.496-502
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    • 1993
  • Spectrophotometric determination of rare earth elements with MTB and the composition ratio were investigated in the presence of surfactants of cetylpyridinium chloride (CPC), Triton X-100, dodecyltrimethylammonium bromide (DTMAB) and cetyltrimethylammonium bromide (CTMAB) at pH 6.5. The colour development between MTB and rare earths in the presence of cationic surfactants was very stable and more sensitive than that in the absence of surfactants. The largest absorbance increase was provided by CPC, which was therefore chosen for determination of rare earth elements. REE-MTB-CPC complex has absorption maxima at 650 nm and obeys the Beer's law in the range of 0∼100 ng/ml. Molar absorptivity is $6.6{\sim}9.4{\times}10^4\;mol^{-1}l\;cm^{-1}$.

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