• Physical Therapy Korea
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• v.24 no.1
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• pp.51-60
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• 2017

Background: In cerebral palsy (CP), parents' quality of life and rearing attitude are considered possible factors that influence patients' quality of life, function level, and performance in daily activities. Despite these facts, little attention has been given to demonstrate a relation between these factors. Objects: This study aimed to demonstrate the relationship between parents' quality of life, functional level, and performance in daily activities, the quality of life of school-aged children with spastic CP. Methods: This study included 24 parents of school-aged children with spastic CP. The KIDSCREEN-52 and World Health Organization quality of life (WHOQOL)-BREF questionnaires (including physical, psychological, social, and environmental domains) were used as research tools to assess the quality-of-life profiles of the children with spastic CP and those of their parents, respectively. In addition, the function levels and performance in daily activities of the children with CP were assessed by using the gross motor function measure (GMFM) and modified Barthel index (MBI). Correlation and multiple regression analyses were performed to clarify the determinants of the quality of life of the children with CP. Results: The KIDSCREEN-52 score correlated with the total score (r=.735, p<.01) and all domains of the WHOQOL-BREF questionnaire (physical: r=.542, p<.01; psychological: r=.690, p<.01; social: r=.568, p<.01; and environmental: r=.783, p<.01). In addition, significant correlation was found between the KIDSCREEN-52 and MBI scores (r=.411, p<.05), and between the MBI and GMFM scores (r=.427, p<.05). After controlling for age, gender, paralytic type, GMFM, and MBI, the WHOQOL-BREF score ($R^2=.621$), particularly in the environmental domain ($R^2=.699$), remained independently related to the quality of life of the children. Conclusion: These findings suggest that the quality of life of school-aged children with spastic CP can be influenced by the quality of life of their parents. This study provides useful information for future studies to investigate the quality of life of children with CP.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.101-101
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• 2005

• BMB Reports
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• v.52 no.6
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• pp.397-402
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• 2019

Middle East respiratory syndrome coronavirus (MERS-CoV) uses the spike (S) glycoprotein to recognize and enter target cells. In this study, we selected two epitope peptide sequences within the receptor binding domain (RBD) of the MERS-CoV S protein. We used a complex consisting of the epitope peptide of the MERS-CoV S protein and CpG-DNA encapsulated in liposome complex to immunize mice, and produced the monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. The western blotting data showed that both monoclonal antibodies detected the S protein and immunoprecipitated the native form of the S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of the antibodies towards the S protein of MERS-CoV virus infected Vero cells. Furthermore, the 506-2G10G5 monoclonal antibody significantly reduced plaque formation in MERS-CoV infected Vero cells compared to normal mouse IgG and 492-1G10E4E2. Thus, we successfully produced a monoclonal antibody directed against the RBD domain of the S protein which could be used in the development of diagnostics and therapeutic applications in the future.

• East Asian mathematical journal
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• v.14 no.1
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• pp.63-76
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• 1998

we consider the hp-version to solve non-constant coefficients elliptic equations $-div(a{\nabla}u)=f$ with Dirichlet boundary conditions on a bounded polygonal domain $\Omega$ in $R^2$. In [6], M. Suri obtained an optimal error-estimate for the hp-version: ${\parallel}u-u^h_p{\parallel}_{1,\Omega}{\leq}Cp^{(\sigma-1)}h^{min(p,\sigma-1)}{\parallel}u{\parallel}_{\sigma,\Omega}$. This optimal result follows under the assumption that all integrations are performed exactly. In practice, the integrals are seldom computed exactly. The numerical quadrature rule scheme is needed to compute the integrals in the variational formulation of the discrete problem. In this paper we consider a family $G_p=\{I_m\}$ of numerical quadrature rules satisfying certain properties, which can be used for calculating the integrals. Under the numerical quadrature rules we will give the variational form of our non-constant coefficients elliptic problem and derive an error estimate of ${\parallel}u-\tilde{u}^h_p{\parallel}_{1,\Omega}$.

• Molecules and Cells
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• v.44 no.3
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• pp.146-159
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• 2021

DNA methylation, and consequent down-regulation, of tumour suppressor genes occurs in response to epigenetic stimuli during cancer development. Similarly, human oncoviruses, including human papillomavirus (HPV), up-regulate and augment DNA methyltransferase (DNMT) and histone deacetylase (HDAC) activities, thereby decreasing tumour suppressor genes (TSGs) expression. Ubiquitin-like containing PHD and RING finger domain 1 (UHRF1), an epigenetic regulator of DNA methylation, is overexpressed in HPV-induced cervical cancers. Here, we investigated the role of UHRF1 in cervical cancer by knocking down its expression in HeLa cells using lentiviral-encoded short hairpin (sh)RNA and performing cDNA microarrays. We detected significantly elevated expression of thioredoxin-interacting protein (TXNIP), a known TSG, in UHRF1-knockdown cells, and this gene is hypermethylated in cervical cancer tissue and cell lines, as indicated by whole-genome methylation analysis. Up-regulation of UHRF1 and decreased TXNIP were further detected in cervical cancer by western blot and immunohistochemistry and confirmed by Oncomine database analysis. Using chromatin immunoprecipitation, we identified the inverted CCAAT domain-containing UHRF1-binding site in the TXNIP promoter and demonstrated UHRF1 knockdown decreases UHRF1 promoter binding and enhances TXNIP expression through demethylation of this region. TXNIP promoter CpG methylation was further confirmed in cervical cancer tissue by pyrosequencing and methylation-specific polymerase chain reaction. Critically, down-regulation of UHRF1 by siRNA or UHRF1 antagonist (thymoquinone) induces cell cycle arrest and apoptosis, and ubiquitin-specific protease 7 (USP7), which stabilises and promotes UHRF1 function, is increased by HPV viral protein E6/E7 overexpression. These results indicate HPV might induce carcinogenesis through UHRF1-mediated TXNIP promoter methylation, thus suggesting a possible link between CpG methylation and cervical cancer.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1999.03b
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• pp.176-179
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• 1999

The high temperature deformation behaviour of 15vol% SiCp/A16061 composites has been studied in the temperature range of 300-50$0^{\circ}C$ and the strain rate range of 0.1-3.0/sec by torsion test. On he basis of the flow stress data the strain rate sensitivity(m) of the material is evaluated and used for calculating the deformation efficiency(η) [η=2m/(m+1)] A domain of dynamic recrystallization(DRX) could be identified in these maps by using the deformation efficiency. The characteristicvs of these results has been investigated with the help of determining the region of optimum hot working condition.

• BMB Reports
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• v.38 no.6
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• pp.739-747
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• 2005

Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.

• Journal of Satellite, Information and Communications
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• v.6 no.1
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• pp.86-96
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• 2011

In this paper we propose a Pre-rake system applied with a frequency domain equalizer in TDD/CDMA multi-code transmission. The Pre-rake system has been well known technique in TDD/CDMA to make a receiver simple. However, it still has residual losses of path diversity and signal to noise ratio (SNR). However, gathering all the residual paths demands an additional hardware such as a rake combiner at the receiver. For the reason Pre/Post-rake system has already been proposed at up/downlink correlated channel conditionunder the assumption of noisier channel. There is a trade-off between the first purpose of Pre-rake that makes hardware simple at the receiver and the performance improvement. From the point the frequency domain equalizer (FDE) can be considered in Pre/Post-rake to supply the receiver with the flexible equalizing methods with rather reduced complexity compared with time domain rake combiner or equalizers. Pre-rake itself increases the number of multipath, which results from the convolution of Pre-rake filter and wireless channel, and FDE must be well matched to Pre/Post-rake, while it considers the relationship of hardware complexity and the performance. In this paper, the Pre-rake/Post-FDE system is introduced at TDD/CDMA multi-code transmission. In addition, the cyclic prefix reduction method in the proposed system is introduced, and the theoretical analysis to the proposed system is given by assuming Gaussian approximation, and finally the numerical simulation results are provided.

• Ocean Systems Engineering
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• v.7 no.1
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• pp.1-19
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• 2017

This paper presents the numerical simulation results for the dynamic responses of two types of submerged floating tunnels (SFT) under wave and/or seismic excitations. Time domain simulations are conducted by the commercial program OrcaFlex (OF) and in-house CHARM3D program (CP). The dynamic performances of a short/rigid/free-end SFT section with vertical and inclined mooring lines are evaluated. The SFT numerical models were validated against Oh et al.'s (2013) model test results under regular wave conditions. Then the numerical models were further applied to the cases of irregular waves or seismic motions. The main results presented are SFT surge/heave motions and mooring tensions. The general trends and magnitudes obtained by the two different software packages reasonably agree to each other along with experimental results. When seabed seismic motions are applied to the SFT system, the dynamic responses of SFTs are small but dynamic mooring tension can significantly be amplified. In particular, horizontal earthquakes greatly increase the dynamic tension of the inclined mooring system, while vertical earthquakes cause similar effect on vertical mooring system.

• The Journal of the Convergence on Culture Technology
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• v.6 no.1
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• pp.381-388
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• 2020

This paper discusses English Hedges or Hedging Expressions on the basis of their morphosyntactic-pragramatic properties within the perspective of sociolinguistics. The term, 'Hedges' for the past decades since Lakoff(1973), has received little attention from the English grammar circles such as morphosyntax and the generative grammar theories. This paper presents a more comprehensive approach to the identification, distributions, functions, and the morphosyntactic properties of English Hedges. The earlier research on English Hedges in the 70's show that hedges are metalinguistic or mitadiscourse expressions which constitute a means for executing Politeness strategy in pragmatics. Nonetheless, research from the interface of syntactic-pragmatics has been scarce. This article suggests a more complex body of English hedges that have not been extensively discussed in the literature. Additionally, their configurational domain is to be proposed as part of the PolP with [±hedged] above CP⁺ (or CP beyond). The ramifications of the current study are suggested in terms of comparative linguistics, EFL/ESL studies of English for global communication, and pragmatics-sensitive machine translation studies in the forseeable future.