• Journal of Biosystems Engineering
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• 제23권2호
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• pp.135-146
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• 1998

The equation to predict the soil temprature of Pusan and Chinju city as a function of time and soil depth for the geothermal energy utilization system and agriculture was devised. The equation was $T(x,t)\;=\;Tm\;-\;To{\cdot}ExP(-{\xi}){\cdot}cos{{\omega}{\cdot}[t-to-x/(2{\cdot}{\alpha}{\cdot}{\omega})^{0.5}]}$ with the soil thermal diffusivity, ${\alpha},\;of\;0.4\;\textrm{m}^2/day,\;0.0375\;\textrm{m}^2/day$ and phase zero point, to, of 24 days, 22.4 days in Pusan and Chinju city, respectively, during ten years from 1987 to 1996. The predicted and measured soil temperatures agreed well with the coefficient of determination of 0.95 at the soil depth of 0.0, 0.5, 1.0, 3.0, 5.0 m. The maximum and minimum temperature in Pusan 3.7, $30.1^{\circ}C$ at soil surface and 14.3, $18.0^{\circ}C$ at the depth of 5.0 m. The total mean temperature of soil in Pusan and Chinju city was about 16.3, $16.0^{\circ}C$, respectively.

• BMB Reports
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• 제40권1호
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• pp.130-134
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• 2007

Haptoglobin (Hp) is a glycoprotein that is produced by hepatic cells and secreted into the circulation. While studying the physiologic functions of Hp, we found that Hp synthesized in THP-1 monocytic cells was largely retained within cells, although Hp is considered a secretory protein. To investigate the molecular mechanism on Hp secretion in THP-1 cells, in the present study, we examined the effect of protein kinase C (PKC) on Hp secretion. When several inhibitors of PKC isoforms were tested, only Rottlerin, a specific inhibitor of PKC-$\delta$, completely blocked Hp secretion from cells to culture medium. To confirm the role of PKC-$\delta$ in Hp secretion, Hp-overexpressing COS7 cells were transiently transfected with a wild-type or a dominantnegative mutant of the PKC-$\delta$ gene. Mutant PKC-$\delta$ significantly inhibited Hp secretion, whereas the wild-type gene slightly increased Hp secretion. These results demonstrate that the PKC-$\delta$ signal is involved in Hp secretion.

• Journal of Oral Medicine and Pain
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• 제20권1호
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• pp.53-65
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• 1995

The growth and synthetic activities of fibroblasts are regulated by cytokines and growth factors derived from activated inflammatory cells. Stimulatory effect of low level laser (LLL) radiation on wound healing seems to be in part due to direct stimulatory action on cell proliferation and synthetic activities of fibroblasts. Also indirect stimulatory effect on the fibroblast function through inflammatory or immune cells is another possible mechanism of biostimulatory action of LLL. This study was performed to determine the growth rate of human gingival fibroblasts obtained biopsy and culture, fibroblast cell line, and immune cell line by using $[^3H]-$ thymidine incorporation test. And gene expression pattern was also analyzed by using the DNA probe such as Hsp70, IL-1$\beta$, MIP-1$\alpha$ and actin cDNA. Proliferation rate of gingival fibroblast was increased by LLL irradiation, but no more effect was added by LPS or IL-1$\beta$ pretreatment Enhanced Hsp70 gene expression was found from gingival fibroblasts and fibroblast cell line COS by LLL irradiation., which was not more increased by LPS or IL-1$\beta$ pretreatment. LLL-irradiated promyelcytic cell line HL-60 and macrophage cell line RAW264.7 showed significant stimulatory effect of proliferation rate when compared with respective control. However there were no changes in growth rate of other immune cell tested in this study, such as B cell line WR19n.l and 230, helper T cell line Jurkat and Hut78, cytolytic T cell line CTLL-r8. By LLL-irradiation Hsp70 gene expression was increased in RAW246.7 and HL-60, not in CTLL-R8. And IL-1$\beta$ and MIP-1$\alpha$ gene expression were induced only from LLL-irradiated RAW264.7. These results led us to presume that LLL radiation may affect to the immune cells, especially to macrophage, through which it might promote wound healing process.

• Journal of Biosystems Engineering
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• 제3권1호
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• pp.1-19
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• 1978

Power tiller is a major unit of agricultural machinery being used on farms in Korea. About 180.000 units are introduced by 1977 and the demand for power tiller is continuously increasing as the farm mechanization progress. Major farming operations done by power tiller are the tillage, pumping, spraying, threshing, and hauling by exchanging the corresponding implements. In addition to their use on a relatively mild slope ground at present, it is also expected that many of power tillers could be operated on much inclined land to be developed by upland enlargement programmed. Therefore, research should be undertaken to solve many problems related to an effective untilization of power tillers on slope ground. The major objective of this study was to find out the travelling and tractive characteristics of power tillers being operated on general slope ground.In order to find out the critical travelling velocity and stability limit of slope ground for the side sliding and the dynamic side overturn of the tiller and tiller-trailer system, the mathematical model was developed based on a simplified physical model. The results analyzed through the model may be summarized as follows; (1) In case of no collision with an obstacle on ground, the equation of the dynamic side overturn developed was: $$\sum_n^{i=1}W_ia_s(cos\alpha cos\phi-{\frac {C_1V^2sin\phi}{gRcos\beta})-I_{AB}\frac {v^2}{Rr}}=0$$ In case of collision with an obstacle on ground, the equation was: $$\sum_n^{i=1}W_ia_s\{cos\alpha(1-sin\phi_1)-{\frac {C_1V^2sin\phi}{gRcos\beta}\}-\frac {1}{2}I_{TP} \( {\frac {2kV_2} {d_1+d_2}\)-I_{AB}{\frac{V^2}{Rr}} \( \frac {\pi}{2}-\frac {\pi}{180}\phi_2 \} = 0 $$ (2) As the angle of steering direction was increased, the critical travelling veloc\ulcornerities of side sliding and dynamic side overturn were decreased. (3) The critical travelling velocity was influenced by both the side slope angle .and the direct angle. In case of no collision with an obstacle, the critical velocity $V_c$ was 2.76-4.83m/sec at $\alpha=0^\circ$, $\beta=20^\circ$ ; and in case of collision with an obstacle, the critical velocity $V_{cc}$ was 1.39-1.5m/sec at $\alpha=0^\circ$, $\beta=20^\circ$ (4) In case of no collision with an obstacle, the dynamic side overturn was stimu\ulcornerlated by the carrying load but in case of collision with an obstacle, the danger of the dynamic side overturn was decreased by the carrying load. (5) When the system travels downward with the first set of high speed the limit {)f slope angle of side sliding was $\beta=5^\circ-10^\circ$ and when travels upward with the first set of high speed, the limit of angle of side sliding was $\beta=10^\circ-17.4^\circ$ (6) In case of running downward with the first set of high speed and collision with an obstacle, the limit of slope angle of the dynamic side overturn was = $12^\circ-17^\circ$ and in case of running upward with the first set of high speed and collision <>f upper wheels with an obstacle, the limit of slope angle of dynamic side overturn collision of upper wheels against an obstacle was $\beta=22^\circ-33^\circ$ at $\alpha=0^\circ -17.4^\circ$, respectively. (7) In case of running up and downward with the first set of high speed and no collision with an obstacle, the limit of slope angle of dynamic side overturn was $\beta=30^\circ-35^\circ$ (8) When the power tiller without implement attached travels up and down on the general slope ground with first set of high speed, the limit of slope angle of dynamic side overturn was $\beta=32^\circ-39^\circ$ in case of no collision with an obstacle, and $\beta=11^\circ-22^\circ$ in case of collision with an obstacle, respectively.

• BMB Reports
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• 제43권1호
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• pp.17-22
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• 2010

In this study, a novel member of BTB-kelch proteins, named KBTBD7, was cloned from a human embryonic heart cDNA library. The cDNA of KBTBD7 is 3,008 bp long and encodes a protein product of 684 amino acids (77.2 kD). This protein is highly conserved in evolution across different species. Western blot analysis indicates that a 77 kD protein specific for KBTBD7 is wildly expressed in all embryonic tissues examined. In COS-7 cells, KBTBD7 proteins are localized to the cytoplasm. KBTBD7 is a transcription activator when fused to GAL4 DNA-binding domain. Deletion analysis indicates that the BTB domain and kelch repeat motif are main regions for transcriptional activation. Overexpression of KBTBD7 in MCF-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE), which can be relieved by siRNA. These results suggest that KBTBD7 proteins may act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.93-99
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• 2011

Amongst a number of potential nonviral vectors, cationic liposomes have been actively researched, with both gemini surfactants and bola amphiphiles reported as being in possession of good structures in terms of cell viability and in vitro transfection. In this study, a cholesterol-based diquaternary ammonium gemini surfactant (Chol-GS) was synthesized and assessed as a novel nonviral gene vector. Chol-GS was synthesized from cholesterol by way of four reaction steps. The optimal efficiency was found to be at a weight ratio of 1:4 of lipid:DOPE (1,2-dioleoyl-L-${\alpha}$- glycero-3-phosphatidylethanolamine), and at a ratio of between 10:1~15:1 of liposome:DNA. The transfection efficiency was compared with commercial liposomes and with Lipofectamine, 1,2-dimyristyloxypropyl-3-dimethylhydroxyethylammonium bromide (DMRIE-C), and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTAP). The results indicate that the efficiency of Chol-GS is greater than that of all the tested commercial liposomes in COS7 and Huh7 cells, and higher than DOTAP and Lipofectamine in A549 cells. Confirmation of these findings was observed through the use of green fluorescent protein expression. Chol-GS exhibited a moderate level of cytotoxicity, at optimum concentrations for efficient transfection, indicating cell viability. Hence, the newly synthesized Chol-GS liposome has the potential of being an excellent nonviral vector for gene delivery.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제50권9호
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• pp.427-433
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• 2001

A linear ultrasonic motor was designed by a combination of the first longitudinal and fourth bending mode, and the motor consisted of a straight aluminum alloys bar bonded with a piezoelectric ceramic element as a driving element. That is,$L_1-B_4$ linear ultrasonic motor can be constructed by a multi-mode vibrator of longitudinal and bending modes. Linear ultrasonic motors are based on an elliptical motion on the surface elastic body, such as bar or plates. In general, the natural resonance frequency of the stator is used as a driving frequency of the motor which provides a large elliptical motion. The corresponding eigenmode of one resonance frequency can be excited twice at the same time with a Phase shift of 90 degrees in space and time. And the rotation can be reversed by changing the phase between the two signals from sin$\omega$t to cos$\omega$t. Moreover, the tangential force pushes the slider(rotor) and, therefore, determines the thrust and speed of the motor. The experimental results of fabrication motors, bimorph-tyPe motor showed more excellent than unimorph-type. The maximum speed of TBL-200, TBL-300, TBL-400, TBL -220, TBL-310 and TBL-420 motors were 0.12, 0.37, 0.39, 0.14, 0.55 and $0.60ms6{-1}$, respectively. And the efficiency were reported 1.15, 7.9, 6.6, 2.36, 10.1 and 16.5%, respectively. That time, output thrust of the motor was a strong(1~2N) and the weight of stator was a lightness(5~7g).

• 한국정보통신학회논문지
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• 제13권4호
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• pp.727-736
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• 2009

본 논문에서는 모바일 벡터 그래픽 가속기용 역코사인 연산 회로를 설계하였다. 모바일 그래픽스 응용은 기존 데스크 톱 컴퓨터에 비해 면적, 연산 시간, 전력 소모와 정밀도 측면에서 제약이 크다. 설계한 역코사인 함수 회로는 연산시간과 정밀도 조건을 만족하기 위해 IEEE 표준 부동 소수점 데이터 형식을 사용하며, 계수 테이블을 사용하는 2차 다항식 근사 기법을 채택하였으며, 하드웨어 공유 기법을 통해 면적을 감소시켰다. 역코사인 회로는 약 15,280개의 게이트로 구성되며, $0.35{\mu}m$ CMOS 공정 조건에서 약 125 Mhz의 동작 주파수를 가진다. 7개의 클록 사이클에 역코사인 함수를 구현하므로, 설계된 회로는 약 17.85 MOPS의 연산 성능을 갖고 있어서 OpenVG 프로세서에 적용이 가능하다. 또한 융통성 있는 구조 특성으로 설계된 회로는 ROM 내용의 교체와 속규모의 하드웨어 변경을 통해 지수함수, 삼각함수, 로그 함수와 같은 다른 초월함수에 적용이 가능하다.

• BMB Reports
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• 제40권6호
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• pp.899-910
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• 2007

Extracellular Regulated Kinases (ERK) and Protein Kinase B (Akt) are intermediaries in relaying extracellular growth signals to intracellular targets. Each pathway can become activated upon stimulation of G protein-coupled receptors mediated by $G_q$ and $G_{i/o}$ proteins subjected to regulation by RGS proteins. The goal of the study was to delineate the specificity in which cardiac RGS proteins modulate $G_{q^-}$ and $G_{i/o}$-induced ERK and Akt phosphorylation. To isolate $G_{q^-}$ and $G_{i/o}$-mediated effects, we exclusively expressed muscarinic $M_2$ or $M_3$ receptors in COS-7 cells. Western blot analyses demonstrated increase of phosphorylation of ERK 1.7-/3.3-fold and Akt 2.4-/6-fold in $M_{2^-}/M_{3^-}$ expressing cells through carbachol stimulation. In co-expressions, $M_3/G_q$-induced activation of Akt was exclusively blunted through RGS3s/RGS3, whereas activation of ERK was inhibited additionally through RGS2/RGS5. $M_2/G_{i/o}$ induced Akt activation was inhibited by all RGS proteins tested. RGS2 had no effect on $M_2/G_{i/o}$-induced ERK activation. The high degree of specificity in RGS proteins-depending modulation of $G_{q^-}$ and $G_{i/o}$-mediated ERK and Akt activation in the muscarinic network cannot merely be attributed exclusively to RGS protein selectivity towards $G_q$ or $G_{i/o}$ proteins. Counter-regulatory mechanisms and inter-signaling cross-talk may alter the sensitivity of GPCR-induced ERK and Akt activation to RGS protein regulation.

• 대한기계학회논문집
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• 제14권3호
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• pp.725-733
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• 1990

본 연구에서는 Raju등의 연구의 확장으로 일정한 온도로 유지되는 경사진 평 판에 대하여 혼합대류 전 영역에 유효한 부력계수(.xi.)로서 (1+Gr$^{2}$$_{x}$cos.gamma./ Re$^{4}$$_{x}$)$_{-1}$를 적용하여 비상사 경계층 방정식을 유도하였다. 또한 적분 형 경계조건을 가진 편미분 경계층방정식을 유한차분법으로 Keller와 Cebeci의 Box scheme을 6차로 적용하여 수치해석하였다. 부력계수가 큰 영역에서 Mucoglu등의 해 석적 연구결과는 일정한 온도와 45˚의 경사를 유지하는 평판에 대한 Ramachandran의 실험적 연구결과와 약 10%정도 벗어난다. 이에 Ramachandran의 실험적 연구를 해석 적으로 재고찰하였다.하였다.