• 생태와환경
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• 제55권3호
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• pp.212-220
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• 2022

재첩은 하구생태계에 서식하는 종으로, 우리나라에는 섬진강 하구에서 가장 많이 분포하는 것으로 알려져 있다. 섬진강 하구에 서식하는 재첩은 담수와 해수생태계의 변화를 잘 반영하는 종이다. 재첩은 외부형태만으로는 종 동정이 어려운 분류군에 속한다. 본 연구에서는 섬진강 서식 재첩의 종 동정을 위해 형태적 관찰뿐만 아니라 미토콘드리아 DNA의 COI 유전자 기반 DNA 바코딩을 통해 분석하였다. 그 결과 재첩 간 다른 두 종을 확인하였으며, COI 염기서열의 다중배열 분석결과 약 98%의 높은 상동성을 보였다. 재첩과 내 다양한 종들과 계통수 분석으로 계통유 전학적 위치를 확인한 결과, 본 연구에서 사용한 재첩은 C. japonica, C. fluminea와 하나의 계통군으로 묶여졌고 진화적 거리는 0.003 이하로 나타났다. 또한 재첩속인 C. leana와 0.089, C. fluminalis와 0.096으로, 재첩과 내 3종과는 약 0.2로 C. japonica에 비해 상대적으로 진화적 거리가 먼 것으로 나타났다. 계통유전학적 분석을 통해 본 연구에서 사용한 재첩은 C. japonica이고, 그중 한 개체가 C. fluminea인 것으로 확인되었다. 이러한 연구 결과는 DNA 바코딩을 이용한 섬진강 하류 지역에 서식하는 재첩의 COI 시컨스를 통해 계통유전학적 정보를 제공하여 형태학적 분석에 어려움이 있는 종에 대한 중요한 자료로 활용될 것이다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.377-377
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• 2003

We investigated the biogeography of Asian Corbicula using mitochondrial gene sequence variation for Corbicula members sampled from 24 localities of 8 Asian regions. A total of 210 individuals were genetically characterized by examining sequence variations of a 614 bp fragment of the mitochondrial cytochrome oxidase I (COI) gene. Phylogenetic analyses of the COI dataset revealed that Corbicula members are subdivided into two well-supported clades: estuarine and freshwater. (omitted)

• Journal of Species Research
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• 제8권2호
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• pp.225-232
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• 2019

Two feather mites, Bychovskiata hypoleuci Mironov and Ddabert, 1997 and Phyllochaeta interifolia (Mégnin and Trouessart, 1884) are reported for the first time in South Korea. Specimens of these two species were collected from the common sandpiper Actitis hypoleucos. The genera Bychovskiata Dubinin, 1951 and Phyllochaeta Dubinin, 1951 are also new reports for South Korea. Here, we provide morphological descriptions and illustrations of these two species. Additionally, we provide partial sequences of the mitochondrial cytochrome c oxidase subunit I(COI) as DNA barcodes.

• Journal of Species Research
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• 제8권2호
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• pp.215-224
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• 2019

We describe three feather mites recovered from a wood sandpiper Tringa glareola that was stored in a $-20^{\circ}C$ freezer at the Chungnam Wild Animal Rescue Center. These feather mites are reported for the first time in South Korea: Avenzoaria totani (Canestrini, 1978), Ingrassia veligera Oudemans, 1904 and Montchadskiana glareolae Dabert and Ehrnsberger, 1999. In this study, we provide morphological diagnoses and illustrations. Additionally, we provide partial sequences of the mitochondrial cytochrome c oxidase subunit I(COI) gene as molecular characteristics of three species.

• 환경생물
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• 제29권3호
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• pp.138-143
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• 2011

이번 연구에서는 중형저서동물의 생태학적 연구에 사용하는 Ludox와 Rose Bengal을 처리하였을 때, 고정액의 종류에 따른 DNA (mtCOI)의 추출 효율을 비교하고자 하였다. 실험을 위해 저서성 요각류 Tigriopus japonicus s.l.를 실험동물로 사용하였으며, 99% 에탄올과 4%포르말린의 두 종류의 고정액을 사용한 뒤 이들을 각각 대조구로 삼았다. 그리고 (1) Ludox HS40, (2) Rose Bengal, (3) Ludox HS40+Rose Bengal을 각각 시료와 반응시킨뒤, mtCOI 유전자를 추출하였다. 이후 PCR을 진행하고 산물을 전기영동하여 유전자의 증폭여부를 확인하였다. 또한 모든 실험은 30회 반복하여 실험간의 결과를 비교 하였다. 그 결과, 에탄올의 경우에는 대조구를 포함한 (1), (2), (3)의 실험 모두에서 96% 이상의 효율을 보였지만, 포르말린의 경우에는 대조구에서 27% 증폭되었으며, (1)과 (3)에서 약 3%, (2)에서 약 7%만 증폭되어 두고 정액에 따른 차이가 뚜렷하게 나타났다. 결과적으로 현재의 연구를 통해서 99% 에탄올이 중형저서동물에서 DNA를 추출하는 데 적합한 고정액임을 확인하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제27권1호
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• pp.142-158
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• 2013

The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is widely utilized for greenhouse pollination in South Korea. Understanding the magnitude of genetic diversity and geographic relationships is of fundamental importance for long term preservation and utilization. As a first step, we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to the "DNA barcode" region and the complete internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA from 88 individuals collected in nine South Korean localities. The complete ITS2 sequences were longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated 112-bp long repeats. The 658-bp long mtDNA sequences provided only six haplotypes with a maximum sequence divergence of 0.61% (4 bp), whereas the ITS sequences provided 84 sequence types with a maximum sequence divergence of 1.02% (21 sites). The combination of the current COI data with those of published data suggest that the B. ignitus in South Korea and China are genetically a large group, but those in Japan can be roughly separated into another group. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population were found to exist among the South Korean populations of B. ignitus, which suggests panmixia. This finding is consistent with our understanding of the dispersal capability of the species.

• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1558-1564
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• 2018

Objective: We report monitoring conservation effect for a Chinese indigenous chicken (Langshan) breed using major histocompatibility complex (MHC) and DNA barcords. Methods: The full length of MHC B-G gene and mitochondrial cytochrome oxidase I (COI) gene in generations 0, 5, 10, 15, 16, and 17 was measured using re-sequencing and sequencing procedures, respectively. Results: There were 292 single nucleotide polymorphisms of MHC B-G gene identified in six generations. Heterozygosity (He) and polymorphic information content (PIC) of MHC B-G gene in generations 10, 15, 16, and 17 remained stable. He and PIC of MHC B-G gene were different in six generations, with G10, G15, G16, G17 >G5>G0 (p<0.05). For the COI gene, there were five haplotypes in generations 0, 5, 10, 15, 16, and 17. Where Hap2 and Hap4 were the shared haplotypes, 164 individuals shared Hap2 haplotypes, while Hap1 and Hap3 were the shared haplotypes in generations 0 and 5 and Hap5 was a shared haplotype in generations 10, 15, 16, and 17. The sequence of COI gene in 6 generations was tested by Tajima's and D value, and the results were not significant, which were consistent with neutral mutation. There were no differences in generations 10, 15, 16, and 17for measured phenotypic traits. In other generations, for annual egg production, with G5, G10, G15, G16, G17>G0 (p<0.05). For age at the first egg and age at sexual maturity, with G10, G15, G16, G17>G5>G0 (p<0.05). Conclusion: Combined with the results of COI gene DNA barcodes, MHC B-G gene, and phenotypic traits we can see that genetic diversity remained stable from generations 10 to 17 and the equimultiple random matching pedigrees conservation population conservation effect of Langshan chicken was effective as measured by these criteria.

• 한국패류학회지
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• 제20권2호
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• pp.171-181
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• 2004

백합 과 (family Veneridae) 패류, Saxidomus purpuratus (개조개), Meretrix lusoria (백합), Meretrix petechialis (말백합), Pitar sulfureum (쇠백합), Ruditapes philippinarum (바지락), Ruditapes variegata (애기바지락), Gomphina aequilatera (대복), Cyclina sinensis (가무락), Dosinella corrugata (주름떡조개) 등 9종과 outgroup으로 Corbicula fluminea (재첩) 를 이용하여 primer로 017 (5'-GGTCAACAAATCATAAAGATATTGG-3'), 018 (5'-TAAACTTCAGGGTGACCAAAAAATCA-3') 를 사용하여 부분적인 mitochondrial cytochrome oxidaxe subunit Ⅰ 유전자에 대한 염기서열을 얻었다. 이밖에도 GenBank에 보고된 Meretrix lamarki, Ruditapes philippinarum, Mercenaria mercenaria 등 3 종에 대한 mCOI 염기서열을 얻어서 maximum parsimony와 neighbor-joining방법으로 분석을 시행하였다. samarangiinae에 속한 3종의 패류 (Ruditapes philippinarum, Ruditapes variegata, Gomphina aequilatera)는 단계통을 보였다. Meretrix lusoria와 M. petechialis는 동일 조상을 갖고 있었으며 Pitarinae에 속한 Saxidomus purpuratus와 CYclinae에 속한 Cyclina sinensis와 동일 조상을 갖는 종들로 나타났다. Pitarinae의 Pitar sulfureum 과 Saxidomus purpuratus는 각기 다른 분지를 이루고 있었다.

• Molecules and Cells
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• 제25권4호
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• pp.510-522
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• 2008

To construct the molecular systematics of the genus Megoura (Hemiptera: Aphididae), DNA based-identification was performed using four mitochondrial and three nuclear DNA regions: partial cytochrome c oxidase I (COI), partial tRNA-leucine + cytochrome c oxidase II (tRNA/COII), cytochrome b (CytB), partial 12S rRNA + tRNA-valine + 16S rRNA (12S/16S), elongation factor-1 alpha ($EF1{\alpha}$), and the internal transcribed spacers 1 and 2 (ITS1, ITS2). Pairwise sequence divergences between taxa were compared, and phylogenetic analyses were performed based on each DNA region separately, and the combined datasets. COI, CytB, $EF1{\alpha}$, ITS1, and ITS2 were relatively effective in determining species and resolving their relationships. By contrast, the sequences of tRNA/COII and 12S/16S were not able to separate the closely related species. CytB and $EF1{\alpha}$ gave better resolution with higher average sequence divergences (4.7% for CytB, 5.2% for $EF1{\alpha}$). The sequence divergence of COI (3.0%) was moderate, and those of the two ITS regions (1.8% for ITS1, 2.0% for ITS2) were very low. Phylogenetic trees were constructed by minimum evolution, maximum parsimony, maximum likelihood, and Bayesian phylogenetic analyses. The results indicated that the phylogenetic relationships between Megoura species were associated with their host preferences. Megoura brevipilosa and M. lespedezae living on Lespedeza were closely related, and M. nigra, monophagous on Vicia venosa, was rather different from M. crassicauda, M. litoralis, and M. viciae, which are oligophagous on Lathyrus and Vicia. The three populations of M. crassicauda formed a clade separated from M. litoralis and M. viciae. Nevertheless M. litoralis and M. viciae, which are morphologically similar, were not separated due to negligible sequence divergence. We discuss the phylogenetic relationships of the Megoura, and the usefulness of the seven DNA regions for determining the species level phylogeny of aphids.

• Animal cells and systems
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• 제16권2호
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• pp.145-153
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• 2012

The pear psylla, $Cacopsylla$ $pyricola$ (Hemiptera: Psyllidae), is a serious insect pest of commercial pear crops. The species, which resides on pear trees throughout its life cycle, is rapidly spreading in some regions of the world. The population genetic structure of the species collected from several pear orchards in Korea was studied to understand the nature of dispersal and field ecology of the species. The 658-bp region of mitochondrial COI gene and the 716-bp long complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA were sequenced. Unlike other previously studied insect pests, the COI-based genetic diversity of the pear psylla was extremely low (maximum sequence divergence of 0.15%). This finding allowed us to conclude that the species may have been introduced in Korea relatively recently. ITS2 sequence-based analyses of phylogeny, population differentiation, gene flow, and hierarchical population structure all concordantly suggested that the pear psylla populations in Korea are neither genetically isolated nor hampered for gene flow. These genetic data are concordant with the dispersal of an overwintering winterform morph outside the non-pear habitat in the fall.