• Korean Journal of Microbiology
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• v.30 no.2
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• pp.101-107
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• 1992

Cytochrome c oxida5e from chemotrophically grown R p , geliitinosu was purified by cytochrome c affinity chromatography and DEAE-Sephacel ion exchange chromatography. The molecular weight of the cytochrome c oxidase was approximately 110.000 Da by sephacryl s-300 gel chromatography and approximately 52, 000 Da by SDS-gel electrophoresis, respectively. Therefore. cytochrolne c oxidase of Rps. gehtinosu seems to be dimer. The cytochrome c oxidasc was very sensitive to temperature. It's Km and Vmax were 20 pM and 44 unitlmg protein for horsc heart cytochrome c as a substrate. respectively, and its optimum pH and temperature were 6.4 and 25$^{\circ}$C. respectively. The absorption peaks of the reduced cytochrome c oxidase showed at 554 nm, 523 nm. and 422 nm. The activiiy of cytochrome c oxidase was inhibited by KCN, and NaN3, but not by CO, antimycir~ A. and myxothiazol. The cytochrome c-551 was produced either in phototrophically or chemotrophically grown Rps. gelaiinosci. The rcduced cytochrome c-551 was oxidized by b-type cytochrome c oxidase from Rp.v. gc.lrtino.sc~. Km and Vmax of cytochrome c oxidase was 26 pM and 31 unitlnlg protein For cytochrome c-551 as a substrate. respectively. Thercfore. thc electron transfer chain of chemotrophically grown Rps. glatinosa seems lo be ubiquinol cytochrome bc, complex -'cytochrome c-55lMb-type cytochrome c oxidase+02.

• Journal of Species Research
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• v.3 no.1
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• pp.27-34
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• 2014

Two species of Tortanus (Eutortanus) are newly recorded from shallow Korean waters: T. (E.) vermiculus Shen, 1955 and T. (E.) komachi Itoh, Ohtsuka and Sato, 2001. As a result of this study, five species are reported in the subgenus of the family Tortanidae in Korea. The sequence of cytochrome c oxidase subunit 1 (CO1) is also provided as a molecular characteristic.

• Applied Microscopy
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• v.31 no.1
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• pp.59-70
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• 2001

The present study was to elucidate the cytochemical study on the cytochrome oxidase (CO) activity and myocardial adaptation to treadmill exercise in rat. The three month Sprague-Dowley male $(150{\pm}10g)$ were used in experimental animal. The experimental groups were divided into 2 groups: the normal sedentary group and the treadmill exercise group. On each 1st and 3rd day, 1st, 4th, 8th, and 12th experimental week four rats of each group were sacrificed for tests. The morphometrical measurements were used to evalute the change of heart weight, rate of myocardial fibers to capillaries, and cytochemical study of CO activities, using light and electron microscopy. The results were as follows: The heart weights were more increased in the treadmill exercise group than those of their sedentary group. The rate of myocardial fibers to capillaries were not changed in sedentary group, but those were significantly from 4th weeks in the treadmill exercise group. The CO activity was not changed in sedentary group, but increased in treadmill exercise group after 1 st week. I and III types of CO activity were increased In sedentary group, in contast to II and III types in treadmill exercise group on electron micrographic study. These results suggest that, the treadmill exercise-induced changes in CO activity and rate of myocardial fibers to capillaries appear to be related to exercise, and the adaptive response seems to occurs from 4th week of treadmill exercise.

• Journal of Environmental Science International
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• v.28 no.1
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• pp.75-84
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• 2019

Cytochrome c oxidase subunit II gene(CO II gene) is subunit of cytochrome oxidase, which is complex IV of mitochondria electron transport system. It has been frequently used in molecular phylogenetic studies because the speed of its DNA variation is faster than that of nucleus. It is especially useful in phylogenetic study of molecular biology in insects. In this study, we cloned and sequenced CO II gene of mitochondria DNA from Rhabditidae family nematode. Our results showed that this gene is comprised of 696 base pairs(bp). In the analysis of similarity of this gene with other known genes of 14 species of nematodes in Rhabditida order, we identified that this gene has high similarity with that of Caenorhabditis briggsae(86.0%) and C. elegans(85.6%) in Rhabditidae family. On the meanwhile, it has very low similarity with that of Angiostrongylus cantonensis(31.8%) in Angiostrongylidae family and Metastrongylus salmi(31.6%) in Metastrongylidae family. Based on the results of this study, we suggest that this nematode is closely related with that of Caenorhabditis genus in Rhabditidae family.

• Korean journal of applied entomology
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• v.59 no.3
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• pp.217-231
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• 2020

In 2019, the sex pheromones of Spodoptera frugiperda were used to examine moth trapping in cornfields in Gochang, Korea. Four types of traps were prepared, two funnel-types and two delta-types, each baited with 300 or 1000 ㎍ of a two-component (2C) blend of synthetic sex pheromones [100% (Z)-9-tetradecenyl acetate (Z9-14Ac) and 2% (Z)-7-dodecenyl acetate (Z7-12Ac)]. The greatest number of S. frugiperda were captured in the 300 ㎍ funnel-type trap (first catch: August 6). Large numbers of Mythimna loreyi (a non-target) were also caught in the funnel-type traps. Two wing-type traps were baited with 1000 ㎍ of the 2C blend or a four-component (4C) blend [100% (Z)-9-tetradecenyl acetate, 8% (Z)-11-hexadecenyl acetate (Z11-16Ac), 2% (Z)-7-dodecenyl acetate, and 1% (Z)-9-dodecenyl acetate (Z9-12Ac)] and the capture efficiency was assessed. Low numbers of S. frugiperda were captured regardless of the blend, and more M. loreyi were captured using the 4C blend. The two intraspecies groups clustered separately in a phylogenetic tree constructed using partial sequences (1004 bp) of cytochrome c oxidase subunit 1 (CO1). Of the 70 S. frugiperda captured in the pheromone traps, 66 belonged to CO1-RS (CO1 rice-strain) and 4 to CO1-CS (CO1 corn-strain). Twelve consistent single nucleotide polymorphisms (SNPs) were identified in CO1 between the CO1-RS and CO1-CS groups of S. frugiperda. Of the 73 S. frugiperda, 4 had the same SNP pattern as the CO1-CS group (including the corn strain) and 69 had the same SNP pattern as the CO1-RS group (including the rice strain).

• Parasites, Hosts and Diseases
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• v.56 no.6
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• pp.583-588
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• 2018

Although widely studied, the natural diversity of the hard tick is not well known. In this study, we collected 194 sequences from 67 species, covering 7 genera of hard tick. The 5' region of the mitochondrial cytochrome c oxidase subunit 1 region (586 bp) has been used to investigate intra- and inter-species variation and the phylogenetic tree of neighbor joining method has been used for assessment. As a result, by comparing the K2P-distance of intra- and interspecies, 30 samples (15.2%) shown that interspecies distance was larger than the minimum interspecfic distance. From the phylogenetic analysis, 86.8% (49) of the species were identified correctly at the genus level. On deeper analysis on these species suggested the possibility of presence cryptic species. Therefore, further work is required to delineate species boundaries and to develop a more complete understanding of hard tick diversity over larger scale.

• Animal Systematics, Evolution and Diversity
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• v.30 no.1
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• pp.26-32
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• 2014

Cleantioides planicauda (Benedict, 1899) has previously been recorded in China and Japan. In the present study, to our knowledge, we report for the first time the occurrence of this species in South Korea. Here, we provide detailed descriptions and illustrations of the diagnostic characteristics of C. planicauda. Further, for the first time, we determined the partial sequence of mitochondrial cytochrome c oxidase subunit 1 (CO1) gene of C. planicauda for molecular characteristic. Additionally, we provide a key to the Korean species and distribution of the genus Cleantioides. As a result of this study, four species of the genus Cleantioides, including C. planicauda, have now been recorded in South Korea.

• Korean Journal of Environmental Biology
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• v.33 no.2
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• pp.177-181
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• 2015

Two marine isopods, Pentias hayi Richardson, 1904 and P. thompsoni Collinge, 1916 were verified as unrecorded species in South Korea. The genus Pentias is reported for the first time in South Korea. These two isopods were collected from Heuksan-do Island and Ulleung-do Island from South Korea, respectively. We provide descriptions of the diagnostic characteristics, illustrations of two species and their partial sequences of the mitochondrial cytochrome c oxidase subunit 1 (CO1) for molecular characteristics.

• Korean Journal of Environmental Biology
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• v.33 no.2
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• pp.182-188
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• 2015

Three marine isopods, Symmius yamaguchiensis Shimomura, 2008, Colanthura pigmentata Kensley, 1980 and Synidotea nipponensis Nunomura, 1985 are reported for the first time in South Korea. The family Chaetiliidae, and genera Symmius and Colanthura are new to South Korea. We provide descriptions of the diagnostic characteristics, illustrations of three species and their partial sequences of the mitochondrial cytochrome c oxidase subunit 1 (CO1) for molecular characteristics.

• Animal Systematics, Evolution and Diversity
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• v.31 no.2
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• pp.135-138
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• 2015

An asteroid specimen was collected in adjacent water of Gisamun, Gangwon-do in the East Sea, Korea at a depth of 170 m by fishing net at May 2013. It was identified as Nearchaster (Nearchaster) pedicellaris (Fisher, 1910) belonging to family Benthopectinidae of order Notomyotida, which was new to the Korean fauna. The genus, family, and order of this species were also new to Korean waters. The partial sequence of mitochodrial cytochrome c oxidase subunit 1 (CO1) was determined for the first time and registered at GenBank.