Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Cloning and Sequencing of the Mitochondrial Cytochrome c Oxidase Subunit II Gene from Rhabditidae Family Nematode

Rhabditidae과 선충의 CO II 유전자 클로닝 및 염기서열 분석

  • Lee, Sang Mong (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Son, Hong Joo (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Kim, Keun Ki (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Hong, Chang Oh (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Park, Hyean Cheal (Department of Life Science & Environmental Biochemistry, Pusan National University)
  • 이상몽 (부산대학교 생명환경화학과) ;
  • 손홍주 (부산대학교 생명환경화학과) ;
  • 김근기 (부산대학교 생명환경화학과) ;
  • 홍창오 (부산대학교 생명환경화학과) ;
  • 박현철 (부산대학교 생명환경화학과)
  • Received : 2018.10.31
  • Accepted : 2018.12.13
  • Published : 2019.01.31
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Abstract

Cytochrome c oxidase subunit II gene(CO II gene) is subunit of cytochrome oxidase, which is complex IV of mitochondria electron transport system. It has been frequently used in molecular phylogenetic studies because the speed of its DNA variation is faster than that of nucleus. It is especially useful in phylogenetic study of molecular biology in insects. In this study, we cloned and sequenced CO II gene of mitochondria DNA from Rhabditidae family nematode. Our results showed that this gene is comprised of 696 base pairs(bp). In the analysis of similarity of this gene with other known genes of 14 species of nematodes in Rhabditida order, we identified that this gene has high similarity with that of Caenorhabditis briggsae(86.0%) and C. elegans(85.6%) in Rhabditidae family. On the meanwhile, it has very low similarity with that of Angiostrongylus cantonensis(31.8%) in Angiostrongylidae family and Metastrongylus salmi(31.6%) in Metastrongylidae family. Based on the results of this study, we suggest that this nematode is closely related with that of Caenorhabditis genus in Rhabditidae family.

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References

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