• Title/Summary/Keyword: CN Method

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Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site (HPLC에 의한 산지별 한국산 석류과피 중 항산화화합물의 함량분석)

  • Kwak, Hye-Min;Jeong, Hyun-Hee;Song, Bang-Ho;Kim, Jong-Guk;Lee, Jin-Man;Hur, Jong-Moon;Song, Kyung-Sik
    • Applied Biological Chemistry
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    • v.48 no.4
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    • pp.431-434
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    • 2005
  • The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.

A Study of the Stability and Moisturizing Effect of Non-Animal Cholesteric Liquid Crystal (비동물성 콜레스테릭 액정의 안정성과 보습효능에 관한 연구)

  • Woo, Byoung Young;Min, Dae Jin;Baek, Heung Soo;Kim, Shin Hyoung;Hwang, Joon Young;Park, Young Ho;Lee, John Hwan;Shin, Song Seok
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.2
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    • pp.141-153
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    • 2014
  • This study is about the development of cholesteric liquid crystals (CLCs), which are highly applicable to cosmetics formulation. The CLCs made from non-animal origin were chosen not only because they are free of animal viruses but also because they give a sense of security to the cosmetic consumers. Three kinds of new cholesteric derivatives (CI, CC, CN) were synthesized using non-animal cholesterol [NAB cholesterol (ARCH LONZA)], which was originally made by fermentation process. To develop high applicability to cosmetics formulation, we attempted to find out the optimum compositions in which CLCs can maintain their color over a wide range of temperature. The CLCs in 41 different compositions were prepared by the combinations of three cholesteric derivatives and a visual evaluation method was employed to determine the range of temperature at which the CLCs display their color. The 205 UV-VIS spectral data obtained from 41 CLCs at various temperatures were simultaneously analyzed to investigate the critical factors affecting the characteristics of the CLCs. Results showed that A4, A5, A6 and A7 were the best compositions to cosmetics formulation. A6 composition had a great moisturizing effect by the artificial skin test ($AmoReSkin^{TM}$).

Assessment of Hydrological Impact by Long-Term Land Cover Change using WMS HEC-1 Model in Gyeongan-cheon Watershed (WMS HEC-1 모형을 이용한 경안천 유역의 경년 수문변화 분석)

  • Lee, Jun-Woo;Kwon, Hyung-Joong;Shin, Sha-Chul;Kim, Seong-Joon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.6 no.1
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    • pp.107-118
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    • 2003
  • The purpose of this study is to assess the hydrological impact on a watershed from long-term land cover changes. Gyeongan-cheon watershed($558.2km^2$) was selected and WMS(watershed modeling system) HEC-1 model was adopted as an evaluation tool. To identify land cover changes, five Landsat images(1980/2/15, 1986/4/15, 1990/4/26, 1996/4/26, 2000/5/17) were selected and analyzed using maximum likelihood method. As a result, urban areas have increased by 5.6% and forest areas have decreased by 6.1% between 1980 and 2000. SCS curve number increased by 9.8. To determine model parameters and evaluate HEC-1 model, five storm events(1998/5/2, 1998/8/23, 1998/9/30, 1999/5/3, 2000/7/29) were used. The simulated stream flow agreed well with the observed one with relative errors ranging from 9% to 36%. For 254 mm daily rainfall of 30 years frequency, due to the increase of urban areas peak flow increased by $455m^3/sec$ and the time of peak flow reduced about four hours for 20 years land cover changes.

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Determination of Model Parameters of Surface Cover Materials in Evaluation of Sediment Reduction and Its Effects at Watershed Scale using SWAT (토양유실 저감을 위한 지표피복 저감효과 변수 결정 및 SWAT 모형 유역단위 효과 분석)

  • Kum, Donghyuk;Jang, Chun Hwa;Shin, Min Hwan;Choi, Joong-Dae;Kim, Bomchul;Jeong, Gyo-Cheol;Won, Chul Hee;Lim, Kyoung Jae
    • Journal of Korean Society on Water Environment
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    • v.28 no.6
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    • pp.923-932
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    • 2012
  • The purpose of this study was to determine parameters of surface cover materials and evaluation the effects on runoff and sediment reductions with rice straw mat with PAM at watershed scale using the SWAT model. In this study, 1) regression equation of CN for rice straw mat + PAM using SCS curve number method was developed, 2) the USLE P factor, being able to reflect simulation of rice straw mat + PAM in the agricultural field, was estimated for various slope scenarios with VFSMOD-w. Then regression equation for CN and USLE P factor were used as input data in the SWAT model. Assuming rice straw mat + PAM is applied to radish and potato fields, occupying 24% of agricultural fields at the study watershed. Result of direct runoff without rice straw mat + PAM was $65,964,368\;m^3,$ with rice straw mat + PAM, direct runoff was $65,637,336\;m^3$, $327,031.8\;m^3$ reductions compared without it. Also, result of sediment without rice straw mat + PAM was 163,531 ton, with rice straw mat + PAM, sediment was 84,779 ton, 78,752 ton reduction compared without it. This analysis showed that about 48% sediment reductions would be expected with rice straw mat + PAM. As shown in this study, rice straw mat + PAM would be used as an efficient site-specific BMPs to reduce runoff and sediment discharge from field.

Differential Pulse Voltammetric Determination of Iron(III) Ion with a Sodium Dodecyl Sulfate Modified Glassy Carbon Electrode (시차펄스전압전류법에서 도데실황산나트륨이 수식된 유리탄소전극에 의한 선택성 있는 철(III) 이온의 정량)

  • Ko, Young Chun;Kim, Jin Ah;Chung, Keun Ho
    • Analytical Science and Technology
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    • v.10 no.6
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    • pp.427-432
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    • 1997
  • A selective method for the determination of iron(III) ion with a sodium dodecyl sulfate(SDS) modified glassy carbon electrode was proposed. It was based on the electrostatic attraction and complexation of the SDS modifier, $(DS^-)_n-Fe^{3+}$. The determination of iron(III) ion was performed by a differential pulse voltammetry(DPV), and the reduction peak potential of $(DS^-)_n-Fe^{3+}$ was +0.466(${\pm}0.002$)V vs. Ag/AgCl. For the determination of iron(III) ion, a linear calibration curve was obtained within the iron(III) ion concentration range of $0.50{\times}10^{-5}{\sim}10{\times}10^{-5}mol/L$, and the detection limit was $0.14{\times}10^{-5}mol/L$. $Cu^{2+}$, $Ni^{2+}$, $Co^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Mn^{2+}$ showed little or no effect on the determination of iron(III) ion, respectively. But, ion such as each $CN^- $ and $SCN^-$ interfered seriously.

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Cure Monitoring of Am Epoxy-Anhydride System by Means of Fluorescence Spectroscopy (형광분석기를 이용한 에폭시-산무수물계의 경화 모니터링)

  • 조동환;김득수;이종근
    • Polymer(Korea)
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    • v.25 no.2
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    • pp.199-207
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    • 2001
  • In the present study the cure behavior of diglycidyl ether of bisphenol-A(DGEBA) using an anhydride-based hardener in the presence of N,N-dimethyl benzyl amine (BDMA) or 1-cyanoethyl-2-ethyl-4-methyl imidazole (2E4MZ-CN) as an accelerator has been monitored and interpreted from the viewpoint of photophysical properties by means of fluorescence spectroscopy. To do this, 1,3-bis-(1-pyrene)propane (BPP) was well incorporated in the epoxy resin system by mechanical blending. The BPP probe, which is very sensitive to conformational change of the molecule influenced by the surrounding medium, successfully formed intramolecular excimer fluorescence. It is susceptible to the micro-viscosity or local viscosity and molecular mobility according to the epoxy cure. The cure behavior was explained with monomer fluorescence intensity ($I_{M}$ ), excimer fluorescence intensity ($I_{E}$ ) and $I_{M}$ /$I_{E}$ ratio as a function of cure time, cure temperature and accelerator. The present work agreed with the previous report on the cure behavior of an epoxy-anhydride system studied using DSC or torsion pendulum method. This study also suggests that the use of fluorescence technique may provide information on cure behavior of a thermosetting resin in a low temperature region, which has not been well interpreted by other analytical methods.

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A Predictive Model of Health Promotion Behavior in Nursing Students (간호대학생의 건강증진행위 예측모형)

  • Oh, Jae-Woo;Moon, Young-Sook
    • Journal of Digital Convergence
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    • v.12 no.10
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    • pp.391-403
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    • 2014
  • This study seeks to carry out a literary review of preceding studies and the health improvement model of Pender(1987) on university students majoring in nursing to explain the health improvement behaviors and identify the factors that affect their activities to provide a framework for developing a more effective nursing mediation method that promotes health improvement behaviors. The study subjects were 204 university students majoring in nursing who have had clinical practice experience. The period for data collection was from April 1to May 30, 2014 and a total of 204 copies of the questionnaire were used for analysis. For the collected data, frequency analysis, percentage, ANOVA, t-test and correlation analysis were conducted using SPSS, LISREL, and path analyss was done for hypothesis testing. The overall index of hypothesis model showed a good congruence as ${\chi}^2=.06$(p=.812), df=1, ${\chi}^2(df)=.000$, GFI=0.97, AGFI=1.0, SRMR=.002, NFI=0.947, NNFI=0.957, RMSEA=0.016, CN=266. Looking at the verification of the hypothesis presented in the model, the variables that affect health improvement behaviors were perceived disability, perceived self-efficacy, perceived social support, while stress from clinical practice, perceived health status, persistence and perceived benefits did not affect health improvement behaviors.

Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens

  • Wang, Shuai;Zhao, Guang-Wei;Wang, Wang;Zhang, Zhen-Chao;Shen, Bo;Hassan, I.A.;Xie, Qing;Yan, Ruo-Feng;Song, Xiao-Kai;Xu, Li-Xin;Li, Xiang-Rui
    • Parasites, Hosts and Diseases
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    • v.53 no.2
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    • pp.155-162
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    • 2015
  • Toxoplasma gondii is a protozoan parasite with a broad range of intermediate hosts. Chickens as important food-producing animals can also serve as intermediate hosts. To date, experimental studies on the pathogenicity of T. gondii in broiler chickens were rarely reported. The objective of the present study was to compare the pathogenicity of 5 different T. gondii strains (RH, CN, JS, CAT2, and CAT3) from various host species origin in 10-day-old chickens. Each group of chickens was infected intraperitoneally with $5{\times}10^8$, $1{\times}10^8$, $1{\times}10^7$, and $1{\times}10^6$ tachyzoites of the 5 strains, respectively. The negative control group was mockly inoculated with PBS alone. After infection, clinical symptoms and rectal temperatures of all the chickens were checked daily. Dead chickens during acute phage of the infection were checked for T. gondii tachyzoites by microscope, while living cases were checked for T. gondii infection at day 53 post-inoculation (PI) by PCR method. Histopathological sections were used to observe the pathological changes in the dead chickens and the living animals at day 53 PI. No significant differences were found in survival periods, histopathological findings, and clinical symptoms among the chickens infected with the RH, CN, CAT2, and CAT3 strains. Histopathological findings and clinical symptoms of the JS (chicken origin) group were similar to the others. However, average survival times of infected chickens of the JS group inoculated with $5{\times}10^8$ and $1{\times}10^8$ tachyzoites were 30.0 and 188.4 hr, respectively, significantly shorter than those of the other 4 mammalian isolates. Chickens exposed to $10^8$ of T. gondii tachyzoites and higher showed acute signs of toxoplasmosis, and the lesions were relatively more severe than those exposed to lower doses. The results indicated that the pathogenicity of JS strain was comparatively stronger to the chicken, and the pathogenicity was dose-dependent.

A Study on the Analysis and Evaluation of Vulnerability Index for the Management of Nonpoint source in SoOak River Watershed (소옥천 유역 비점오염 관리를 위한 취약성 지수분석 및 평가에 관한 연구)

  • KAL, Byung-Seok;MUN, Hyun-Saing;HONG, Seon-Hwa;PARK, Chun-Dong;GIL, Han-nui;PARK, Jae-Beom
    • Journal of the Korean Association of Geographic Information Studies
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    • v.22 no.2
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    • pp.107-120
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    • 2019
  • In this study, the characteristics of watershed and pollution source were investigated in SoOakcheon located in the upper stream of Daecheong Lake. The Dimension Index method was applied to index various watershed and pollution source data. The influence factors of each pollutant source were derived through correlation analysis between selected index and water quality monitoring data. BOD and COD were significantly influenced by population density and land area ratio, T-N by CN and rice area ratio, and T-P by population density and land area ratio, respectively. The discharge load is often used to establish non-point source countermeasures, but there is a difference between the water load and the water load in the lake or river. Therefore, in order to manage non-point pollution efficiently, it is necessary to analyze influential factors with high correlation with water quality and to manage the relevant factors with priority.

Analysis of Cloud Seeding Case Experiment in Connection with Republic of Korea Air Force Transport and KMA/NIMS Atmospheric Research Aircrafts (공군수송기와 기상항공기를 연계한 인공강우 사례실험 분석)

  • Yun-Kyu Lim;Ki-Ho Chang;Yonghun Ro;Jung Mo Ku;Sanghee Chae;Hae-Jung Koo;Min-Hoo Kim;Dong-Oh Park;Woonseon Jung;Kwangjae Lee;Sun Hee Kim;Joo Wan Cha;Yong Hee Lee
    • Journal of Environmental Science International
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    • v.32 no.12
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    • pp.899-914
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    • 2023
  • Various seeding materials for cloud seeding are being used, and sodium chloride powder is one of them, which is commonly used. This study analyzed the experimental results of multi-aircraft cloud seeding in connection with Republic of Korea Air Force (CN235) and KMA/NIMS(Korea Meteorological Administration/National Institute of Meteorological Sciences) Atmospheric Research Aircraft. Powdered sodium chloride was used in CN235 for the first time in South Korea. The analysis of the cloud particle size distributions and radar reflectivity before and after cloud seeding showed that the growth efficiency of powdery seeding material in the cloud is slightly higher than that of hygroscopic flare composition in the distribution of number concentrations by cloud aerosol particle diameter (10 ~ 1000 ㎛). Considering the radar reflectivity, precipitation, and numerical model simulation, the enhanced precipitation due to cloud seeding was calculated to be a maximum of 3.7 mm for 6 hours. The simulated seeding effect area was about 3,695 km2, which corresponds to 13,634,550 tons of water. In the precipitation component analysis, as a direct verification method, the ion equivalent concentrations (Na+, Cl-, Ca2+) of the seeding material at the Bukgangneung site were found to be about 1000 times higher than those of other non-affected areas between about 1 and 2 hours after seeding. This study suggests the possibility of continuous multi-aircraft cloud seeding experiments to accumulate and increase the amount of precipitation enhancement.