• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제9권2호
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• pp.242-248
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• 2006

목 적: 비-가족형, 비-대사성, 비-A, B, C형 바이러스성 신생아 간염은 양호한 경과를 취한다고 알려져 있지만, 장기적인 경과 관찰에 대한 연구가 부족하다. 연자들은 이 질환의 임상적 특징을 연구하여 임상 경과 및 예후 예측에 도움을 얻고자 하였다. 방 법: 1998년 1월부터 2004년 1월까지 생후 3개월 이내에 부산대학교병원에서 신생아 간염으로 진단되었던 환자 중 임상 소견 및 생화학적 검사의 이상 소견이 6개월 이상 지속되었던 34명을 대상(A, B, C형 바이러스성, 대사성, 유전성 신생아 간염은 제외)으로 하여 임상적 소견, 검사실 소견 및 조직학적 소견을 병력지와 조직 슬라이드 분석을 통해 후향적으로 연구를 시행하였다. 결 과: 성비는 2.4 : 1로 남아가 많았고, 진단 시 연령은 생후 1~2개월 사이가 가장 많았다. 혈청 ALT의 최고치의 범위는 광범위(100~1,000 IU/L)하였으나, 300 IU/L 미만이 41%였다. 혈청 직접형 빌리루빈의 최고치는 50%에서 1.0~5.0 mg/dL 사이였다. CMV에 대한 IgM 항체 검사 또는 PCR 검사에서 34%가 양성이었다. 추적 기간의 조건을 충족한 29명 중 11명(37.9%)의 환자가 1년 이내에 ALT 수치가 정상화되었고, 1년 이상 ALT 수치가 증가된 환자 13명 중에서는 2년 이내에 정상화된 경우가 9명(69.2%), 2년 이상 지속적으로 상승된 경우가 4명(30.7%)였다. 2년 이상 지속적으로 ALT 수치의 상승이 있었던 경우 간 조직 검사에서 간문맥 주변 섬유화, 간문맥 염증 및 간소엽 염증 등의 변화가 2년 이내에 회복된 경우보다 심했으나 통계학적인 의미는 없었다. 결 론: 비-가족형, 비-대사성, 비-A, B, C형 바이러스성 신생아 간염은 비교적 예후가 양호하지만 혈청 ALT 수치의 상승이 1년 이상 지속 시 간 손상의 정도에 대한 평가와 만성 간질환에 대한 주의 깊은 관찰이 필요하다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.149.2-150
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• 2003

Occurrences of virus diseases on paprika ( Capsicum annuum var. grossum) were surveyed in Joennam province from 1999 to 2003 and the collected samples showing virus-like symptoms were tested using ELISA. Virus diseases appeared 4.5％, 17.5％, and 4.9％ in 2000, 2002, and 2003, respectively. As the results of investigation of the seasonal incidence with the growing stages of plant, virus was not occurred at seedling stage and was slightly from the planting time to the first harvesting time, but was dramatically increased at the second harvesting time. Virus diseases were more severe on the vinyl house than on the green house. Pepper mild mottle virus (PMMoV) was severely occurred in 2000 but not after that year. Comparing the virus species, Pepper mottle virus (PepMoV) was 35.9％, Broad bean wilt virus (BBWV) was 14.1％, and Cucumber mosaic virus (CMV) was 10.9％ in 2002, and 76.0％, 11.1％, and 2.4％ in 2003, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제13권10호
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• pp.1367-1372
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• 2000

The present study was conducted to evaluate the efficiency of transgenic rabbit production by DNA microinjection using EGFP (Enhanced Green Fluorescent Protein) gene. In this experiment EGFP coding sequences fused to CMV promoter were microinjected into rabbit one-cell embryos, and then GFP expression and gene integration were evaluated in preimplantation embryos and fetuses recovered on day 15 of pregnancy to determine efficiency of transgenic rabbit production. Effect of DNA concentration was also tested on development in vitro following microinjection and transgene integration in fetuses. Development of embryos in vitro was decreased by DNA microinjection, but the rates of pregnancy and implantation were not significantly affected by microinjection. As development progressed in vitro percentage of GFP expression in rabbit embryos was decreased, resulting GFP expression detected in 37.5% of blastocysts. The efficiencies for production of transgenic fetuses were 4.0% and 7.6%, respectively, when $10ng/{\mu}l$ and $20ng/{\mu}l$ of DNA concentration were microinjected. Transgenic fetuses were confirmed by GFP expression and PCR analysis of fetus genomic DNA. These results indicated that DNA microinjection itself damaged embryo development and DNA concentration affected the efficiency of transgenic rabbit production.

• Fisheries and Aquatic Sciences
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• 제7권2호
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• pp.70-75
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• 2004

The CMV promoter driven lacZ reporter gene (pcDNA-lacZ) was constructed and used for DNA immunization study. The expression of the lacZ gene was confirmed in vitro using RTG-2 cell line before using for in vivo study in olive flounder (Paralichthys olivaceus). In the dose response study, the maximum expression of the lacZ gene was found in the group injected with 5 ${\mu} g$ of the plasmid DNA. Kinetic study showed a significantly increased expression of $\beta-galactosidase$ gene at 7 days after injection. Effects of DNA vaccine on specific and nonspecific immune responses such as antibody and NO production were studied and the significant effect was found in olive flounder injected with 10 and 15 ${\mu} g$ DNA (sub optimal dose for lacZ gene expression). Two pro inflammatory cytokine genes, $IL-l\beta$ and $TNF-\alpha$, were also found to be up regulated in the muscle injected with the plasmid, suggesting an induction of local inflammatory response.

• The Plant Pathology Journal
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• 제19권3호
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• pp.166-170
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• 2003

Ninety samples showing mosaic symptoms on soybean (Glycine max) cv. Sukryangputkong were collected from the Cheongsongkun area, Kyungbuk province in Korea. Initially, DAS-ELISA was conducted far detection of Soybean mosaic virus (SMV). Negative samples were chosen at random and mechanically inoculated on soybean cv. Buffalo, which reported not to produce mosaic symptoms when mechanically inoculated with SMV. An isolate of SMV, designated as B-1, from Buffalo showing mosaic and mottle symptoms was used for identification and biological characterization of the causal vim. The purified B-1 isolate had spherical particles of approximately 24nm. It positively reacted with the antiserum against Cowpea mosaic virus (CPMV) but not with Cucumber mosaic virus (CMV) and SMV antisera. CPMV was newly isolated from soybean and had been characterized by host range and by serological and electron microscopic methods. Results of this study suggest that CPMV is the possible cause of mosaic disease in vegetable soybean and that based on sympto-matology, a difference between the typical mosaic and rugose symptoms caused by SMV and CPMV was observed. This is first report of CPMV from soybean in Korea.

• 한국가축번식학회지
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• 제26권4호
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• pp.321-328
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• 2002

The hormone resistin is associated with type II diabetes mellitus in rodent model. Resistin impairs glucose tolerance and insulin action. A new class of anti-diabetic drugs were called thiazolidinediones (TZDs) downreguates a resistin. Resistin gene expression is induced during adipocyte differentiation and resistin polypeptide is secreted by adipocytes. But, the correlation between increased adiposity and resistin remains unknown. The objectives of this study was to clone a mouse resistin CDNA and to generate transgenic mice overexpressing mouse resistin gene. The pCMV-mus/resistin gene was prepared from previous recombinant pTargeT$^{TM}$-mus/resistin by digestion of Bgl II, and has used for microin- jection into pronuclei of one cell embryos. Mouse resistin expression was detected in transgenic F$_1$mice by RT-PCR. The transgenic mouse with resistin gene expression has heavier body weight which was measured higher level of plasma glucose than that of normal mouse. And in diet-induced experiments, in fasting group, resistin expression was higher than that of re-feeding group. This result demonstrates that the resistin gene overexpressing mice may be became to obesity and be useful as an animal disease model to be diabetes caused by insulin resistance of resistin.n.

• Reproductive and Developmental Biology
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• 제32권1호
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• pp.15-20
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• 2008

Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.

• Journal of Power Electronics
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• 제16권6호
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• pp.2258-2271
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• 2016

In this paper, some crucial performance characteristics related to the operational reliability of the post-fault Pulse Width Modulated (PWM) rectifiers, such as line current harmonic distortion, Common Mode Voltage (CMV), and current stress on the capacitors, are fully investigated. The aforementioned performance characteristics of post-fault rectifiers are highly dependent on the utilized space vector modulation (SVM) schemes, which are also examined. Detailed analyses of the three most commonly used SVM schemes for post-fault PWM rectifiers are provided, revealing the major differences in terms of the zero vector synthesis approaches. To compare the performances of the three SVM schemes, the operating principles of a post-fault rectifier are presented with various SVM schemes. Using analytical and numerical methods in the time domain, the performances of the line current distortion, common mode voltage and capacitor current are evaluated and compared for each SVM scheme. The proposed analysis demonstrates that the zero vector synthesis approaches of the considered methods have significant impacts on the performance characteristics of rectifiers. In addition, the advantages and disadvantages of the proposed SVM schemes are discussed. The experimental results verify the effectiveness and validity of the proposed analysis.

• Journal of Pharmaceutical Investigation
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• 제37권1호
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• pp.39-43
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• 2007

Previously, we have reported that PLGA nanoparticles were prepared for sustained release of water-soluble blue dextran and the particle size, in vitro release pattern and encapsulation were modulated by varying polymers. This study was designed to encapsulate plasmid DNA in PLGA nanoparticles and to investigate the effect of Polymers and temperatures. PLGA nanoparticles were fabricated with poloxamer 188 (P188) or poloxamer 407 (P407) by using spontaneous emulsification solvent diffusion method. As a model plasmid DNA, pCMV-Taq2B/1L-18 was encapsulated in PLGA nanoparticles. Then, the particle size, zeta potential and encapsulation efficiency of nanoparticles containing plasmid DNA were investigated. Particle sizes of PLGA nanoparticles prepared with P188 and P407 were in the range of 200-330 nm and 250-290 nm, respectively. Zeta potentials of nanoparticles were negative regardless of nanoparticle compositions. Encapsulation efficiency of P407 nanoparticles prepared at $30^{\circ}C$ was higher than those at other preparation condition. From the results, the PLGA nanoparticles prepared with poloxamers at different temperature, could modulate the particles size of nanoparticles, and encapsulation efficiency of plasmid DNA.

• The Plant Pathology Journal
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• 제15권2호
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• pp.119-123
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• 1999

Chrysanthemums showing leaf mottling were collected from three southern locations in Korea in 1998. Two kinds of viruses were isolated from the leaves and were identified as tomato aspermy virus ch-TAV) and chrysanthemum virus B (ch-CVB), according to their host range, morphology, intracellular location, agar gel double diffusion test, and double-stranded RNA (dsRNA) analysis. The purified ch-TAV was spherical particles of approximately 29 nm in diameter and ch-CVB was filamentous particles of 685 nm long. Inclusion bodies were not observed in ch-TAV and/or ch-CVB infected chrysanthemum. ch-TAV showed positive serological reaction with TAV antiserum (ATCC-127) but not with CMV-pepper antiserum. In dsRNA analysis, four kinds of viral dsRNA were observed on ch-TAV and one viral dsRNA was shown on ch-CVB. Rate of co-infection with TAV and CVB in commercial chrysanthemums was 20.9%. On the other hand, infection with CVB alone was 97.2%. However, chrysanthemums naturally infected with TAV alone were not found.