• 제목/요약/키워드: CM fusion

검색결과 207건 처리시간 0.03초

기하학적 제한 조건에 의한 파티클 필터링 성능 평가 연구 (Representation of Constraint Manifold and its Evaluation for CM-based Particle filter)

  • 이장용;이석한
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2005년도 추계학술발표대회 및 정기총회
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    • pp.639-642
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    • 2005
  • 융합과 필터링(Fusion and Filtering: F/F) 기법은 신호처리, 제어 등 많은 공학분야에서 사용되며 현재 파티클 필터(Particle Filter: PF)가 최근 가장 각광받고 있다. 그러나 비선형 시스템과 모델링 하기 어려운 에러조건 때문에 기존의 파티클 필터조차 제대로 다루지 못하는 공학환경이 존재한다. 이에 파티클 필터뿐만 아니라 칼만 계열(Kalman varieties)의 필터 방법들을 통합할 수 있는 Constraint Manifold(CM) 기반 융합과 필터링 방법이 제안되었다. 본 논문에서는 CM 기반 필터링을 효과적으로 수행할 수 있도록 제한 조건 표현에 대한 방법론을 제시하면 시뮬레이션을 통해 기존 파티클 필터와의 성능 비교를 수행하였다.

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Solid-phase refolding of poly-lysine tagged fusion protein of hEGF and angiogenin

  • Park, Sang-Joong;Ryu, Kang;Chai, Young-Gyu;Kweon, Oh-Byung;Park, Seung-Kook;Lee, Eun-Kyu
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2001년도 추계학술발표대회
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    • pp.197-203
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    • 2001
  • A fusion protein, consisting of human epidermal growth factor as a recognition domain and human angiogenin as a toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as inclusion body in recombinant E. coli, and when the conventional, solution-phase refolding process was used the refolding yield was very low due to severe aggregation, probably due to the opposite surface charge due to vastly different pI values of each domain. Solid-phase refolding process exploiting ionic interactions between the solid matrix and the protein was tried, but the ionic binding yield was very low regardless of the resins and pH conditions used. To provide higher affinity toward the solid matrix, six lysine residues were tagged to the N -terminus of the hEGF domain When the cation exchange resins such as heparin- or CM-Sepharose were used as the matrix, the adsorption capacity increased 2.5-3 times and the subsequent refolding yield increased nearly IS times compared to the conventional process.

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Three-dimensional Self-consistent Particle-in-cell and Monte Carlo Collisional Simulation of DC Magnetron Discharges

  • Kim, Seong-Bong;Chang, Hyon-U;Yoo, Suk-Jae;Oh, Ji-Young;Park, Jang-Sik
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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    • pp.526-526
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    • 2012
  • DC magnetron discharges were studied using three-dimensional self-consistent particle-in-cell and Monte Carlo collisional (PIC-MCC) simulation codes. Two rectangular sputter sources (120 mm * 250 mm and 380 mm * 200 mm target sizes) were used in the simulation modeling. The number of incident ions to the Cu target as a function of position and simulation time was obtained. The target erosion profile was calculated by using the incident ions and the sputtering yields of the Cu target calculated with SRIM codes. The maximum ion density of the ion density distribution in the discharge was about $10^{10}cm^{-3}$ due to the calculation speed limit. The result may be less than one or two order of magnitude smaller than the real maximum ion density. However, the target erosion profiles of the two sputter sources were in good agreement with the measured target erosion profiles except for the erosion profile near the target surface, in which which the measured erosion width was broader than the simulation erosion width.

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A SMA-based actuation system for a fish robot

  • Le, Chan Hoang;Nguyen, Quang Sang;Park, Hoon Cheol
    • Smart Structures and Systems
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    • 제10권6호
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    • pp.501-515
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    • 2012
  • We design and test a shape memory alloy (SMA)-based actuation system that can be used to propel a fish robot. The actuator in the system is composed of a 0.1 mm diameter SMA wire, a 0.5 mm-thick glass/epoxy composite strip, and a fixture frame. The SMA wire is installed in a pre-bent composite strip that provides initial tension to the SMA wire. The actuator can produce a blocking force of about 200 gram force (gf) and displacement of 3.5 mm at the center of the glass/epoxy strip for an 8 V application. The bending motion of the actuator is converted into the tail-beat motion of a fish robot through a linkage system. The fish robot is evaluated by measuring the tail-beat angle, swimming speed, and thrust produced by the tail-beat motion. The tail-beat angle is about $20^{\circ}$, the maximum swimming speed is about 1.6 cm/s, and the measured average thrust is about 0.4 gf when the fish robot is operated at 0.9 Hz.

용액 결정성장하의 Nylon 4,6 의 결정구조 및 열적성질 (Crystal structure and thermal properties of solution crystallized nylon 4,6)

  • 김연철;홍성권
    • 한국재료학회:학술대회논문집
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    • 한국재료학회 1993년도 춘계학술발표회
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    • pp.99-100
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    • 1993
  • Calorimetric (D.S.C) studies were carried out on the nylon 4,6 single crystals grown from 1,4-butanediol solution at various crystallisation temperatures, based on the assessment of the lamellar thickness by small angle x-ray scattering. Samples were annealed mainly ot get rid of residual solvents inside the crystals. The effect of annealing on the crystal perfection is inferred from the measured thermal properties of the crystals. Accordig to the scanning rates less than 80 K/min., D. S C. melting peaks indicate that changes in the internal morphology of nylon 4,6 crystals preapred at different crystallisation temeratures yield a thermodynamic melting temperature. Tm, of 319 $^{\circ}C$, for the infinitely extended crystal thickness (1/ι). The obtained heat of fusion value for the inginite crystal thickness, Ho, was 270 J/g from the plot of measured feat of fusion ($\Delta$Hm) vs. reciprocal crystal thickness (1/ι). based on these values, the fold surface energy, $\delta$e. of 65.4 erg/$\textrm{cm}^2$ was obtained from Hoffman-Waeeks equation. The thermodynamic melting temperature and heat of fusion of the infinite crystal thickness for the solution grow nylon 4,6 single crystals are found to be higher than of the reported corresponding solution grown nylon 6,6 single crystals. pbtained crystallinity from D. S. C measurements ranges from 40 to 50 %, which is close to the reported yalue for the nylon 6,6 single ctystals but lower than we expected.

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대장균 xylA 유전자의 구성적 변이주의 분리 (Isolation of Constitutive Mutant of xylA Gene in Escherichia coli)

  • 소재현;노동현;이인구
    • Current Research on Agriculture and Life Sciences
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    • 제11권
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    • pp.81-89
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    • 1993
  • xylA 유전자의 발현에 관한 xylR 유전자의 조절 메카니즘을 밝히기 위한 연구의 일환으로 xylA 프로모터 하류에 cat 유전자를 삽입시켜 Pxyl-cat-xylA 융합 플라스미드인 pEXC131을 제작하였고 이 플라스미드를 xylA 변이주인DH77로 형질전환시킨 결과 xylose의 유도시에만 Cm 내성과 xylose isomerase활성이 나타났다. pEXC1131/DH77에 NTG를 처리하여 xylose 유도없이도 Cm 내성과 xylose isomerase의 활성을 나타내는 xylA 유전자의 구성적 변이주인 pEXC131-39를 xylR 변이주인 DH60으로 형질전환시킨 균주가 xylose에 의한 유도와 무관하게 Cm 내성 및 xylose isomerase 활성을 가지는 것으로 보아 xylA 유전자의 프로모터부위의 변이에 의한 구성적 변이주임을 확인하였다.

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용접 입열량에 따른 저탄소형 TMCP 구조용 강재의 용접부 충격인성 및 미세조직 변화에 관한 연구 (A Study on the Impact Toughness and Microstructure change for Low carbon TMCP Structural Steel Alloy with Welding Heat Impact)

  • 권순두;이광학;박동환
    • 대한용접접합학회:학술대회논문집
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    • 대한용접접합학회 2004년도 추계학술발표대회 개요집
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    • pp.101-102
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    • 2004
  • This study was investigated on the impact toughness and microstructure of welded metal and heat affected zone for B grade steel. With welding procedures, welding heat inputs applied were 30, 79 and 264 kJ/cm, Prior austenite grain size in coarse zone has increased with increasing welding heat input for B grade steel. The toughness of fusion line zone of Bgrade steel has decreased with increasing welding heat input while the toughness fusion line +3 and +5 mm zone increased contrarily.

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자유낙하에 의한 휴대폰의 충격 해석 (Impact Analysis of Freely Falling Cellular Phone)

  • 강성수;김종수;설균호
    • 한국생산제조학회지
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    • 제22권6호
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    • pp.963-968
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    • 2013
  • In this study, we carried out a structural analysis for determining the stresses acting on the tempered glass of a freely falling cellular phone using ANSYS 13.0, commercial finite element code. We designed a phone heavier than any contemporary cellular phone and performed finite element analysis using the falling speed at a height of 104 cm, which is equal to of the average leg length of Koreans. By determining the maximum stress acting on the phone's tempered glass and frame, we identified the location of large deformation. Furthermore, we evaluated the maximum/minimum equivalent stress and deformation of the reinforced glass and frame as functions of time.

Emission Behavior of Screen Printed CNT Field Emitters for Advanced Lamp Application

  • Leer, Myoung-Bok;Kim, Dae-Jun;Song, Yoon-Ho
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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    • pp.691-692
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    • 2009
  • Screen printable CNT pastes were formulated including conductive nano particles (CNPs) and their properties were investigated with an expectation of stable cold cathodes for advanced lamp application. CNT cathodes showed a turn-on field of 1~1.5V/um, a life time of ~100 hours at an emission current density of 10uA/$cm^2$ for DC-bias. Detailed analysis of measured I-V was carried out by applying Fowler-Nordheim model to trace down the origin of emission property degradation.

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The Use of Bull Round Spermatids for Producing Reconstructed Embryos

  • S.A. Ock;D.O. Kwack;Park, G.J.;S.Y. Choe
    • 한국발생생물학회:학술대회논문집
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    • 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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    • pp.133-133
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    • 2003
  • Recently, sperm has been used as a vector to carry exogenous genes for the production of transgenic animals. However, the success in cattle is low, due to deficiencies in oocyte activation and sperm decondensation caused by high disulphide bond (S=S) content in mature sperm. This study was carried out to develop an effective method for producing transgenic animals with round spermatids (RS). Two methods of embryo production - electric fusion (EC) or intracyto-plasmic injection (IC) and three activation treatments were compared. RS were isolated from bull testes by Percoll density gradients (20, 35, 40, 45 and 90%). Fusion between ooplast and RS was performed with a single DC electric pulse (1.0 KV/cm, 45 sec) in 0.28 M mannitol solution supplemented with 100 M CaCl2 and 100 M MgCl$_2$. (중략)

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