• Title/Summary/Keyword: CLE

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Soil Microbial Community Analysis in Large Patch (Rhizoctonia solani AG2-2 IV) (갈색퍼짐병 발병토양의 미생물 군집 분석)

  • Lee, Jung Han;Min, Gyu Young;Shim, Gyu Yul;Jeon, Chang Wook;Choi, Su min;Han, Jeong Ji;Kwak, Youn-Sig
    • Weed & Turfgrass Science
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    • v.4 no.2
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    • pp.124-128
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    • 2015
  • Large patch, caused by Rhizoctonia solani AG2-2 IV, is a soil-born disease that is the most important of warm season turfgrass such as zoysia and Bermuda grass. This study was conducted to analysis of the soil microbial community structure on large patch. Center of the large patch (CLC), edge (CLE) and healthy (CLH) part of microbial communities were examined using metagenomics in Phylum level. Distribution trends of the rhizosphere microorganisms were similar to the order Proteobacteria, Acidobacteria, Chloroflexi, Firmicutes, Planctomycetes, Gemmatimonadetes, Nitrospira, Cyanobactria and Verrucomicrobia in soil collections. Contrastively Actinobacteria was more 56% abundant in healthy part soil (16%) than in the center (9.28%) or edge (10.84%) parts. Taxonomic distributions were compared among the CLC, CLE and CLH, total 6,948 OTUs were detected in the CLC, 6,505 OTUs for the CLE and 5,537 OTUs were detected in the CLE. Distributions of Actinobacteria OTUs were appeared 615 OTUs in the CLC, 709 OTUs in the CLE and 891 OTUs in the CLH. Among Actinobacteria, 382 OTUs were overlapped in the all soils. Not matched OTUs of CLH (286 OTUs) was detected 23 times higher than CLC (91 OTUs) and CLE (126 OTUs).

Provably secure certificateless encryption scheme in the standard model

  • Deng, Lunzhi;Xia, Tian;He, Xiuru
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.14 no.6
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    • pp.2534-2553
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    • 2020
  • Recently, numerous certificateless encryption (CLE) schemes have been introduced. The security proofs of most schemes are given under the random oracle model (ROM). In the standard model, the adversary is able to calculate the hash function instead of asking the challenger. Currently, there is only one scheme that was proved to be secure in SM. In this paper, we constructed a new CLE scheme and gave the security proofs in SM. In the new scheme, the size of the storage space required by the system is constant. The computation cost is lower than other CLE schemes due to it needs only two pairing operations.

Physicochemical Properties of Cross-linked and Partially Enzymatically Hydrolyzed (CLE) Waxy Rice Starch (가교화 후 효소처리(CLE) 찹쌀 전분의 물리화학적 특성)

  • Yu, Chul;Kim, Sung-Woo;Kim, Chong-Tai;Choi, Sung-Won;Kim, Byung-Yong;Baik, Moo-Yeol
    • Korean Journal of Food Science and Technology
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    • v.40 no.3
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    • pp.290-296
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    • 2008
  • This study examined the physicochemical properties of chemically and enzymatically cross-modified waxy rice starches. The waxy rice starch was cross-linked using phosphorous oxychloride, and then partially hydrolyzed with four commercial ${\alpha}$-amylases (Fungamyl, Termamyl, Liquozyme, Kleistase). Swelling power and the moisture sorption isotherm did not change with cross-modification. Two cross-modified waxy rice starches (hydrolyzed with Termamyl and Liquozyme) showed higher solubilities than native starch and the two other cross-modified starches (hydrolyzed with Fungamyl and Kleistase). In terms of RVA characteristics, the two cross-modified waxy rice starches hydrolyzed with Termamyl and Liquozyme, respectively, had lower peak viscosity, holding strength, and final viscosity than the native starch. However, the two starches hydrolyzed with Fungamyl and Kleistase, respectively, revealed higher peak viscosity, holding strength, and final viscosity than the native starch. No differences were displayed in the X-ray diffraction patterns and DSC thermal characteristics of the cross-modified waxy rice starch as compared to both the native and cross-linked starches, indicating that cross-linking and enzymatic hydrolysis occurred in the amorphous region and did not alter the crystalline region.

Antioxidative and Anti-inflammatory Effects of Extracts from Different Organs of Cirsium japonicum var. ussuriense (엉겅퀴(Cirsium japonicum var. ussuriense) 부위별 추출물의 항산화 및 항염증 효과)

  • Mok, Ji-Ye;Kang, Hyun-Ju;Cho, Jung-Keun;Jeon, In-Hwa;Kim, Hyeon-Soo;Park, Ji-Min;Jeong, Seung-Il;Shim, Jae-Suk;Jang, Seon-Il
    • The Korea Journal of Herbology
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    • v.26 no.4
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    • pp.39-47
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    • 2011
  • Objective: The roots, leaves, flowers, stems and seeds of Cirsium japonicum var. ussuriense are often used in treatment of human diseases such as hemorrhage, blood congestion and inflammation. Focusing our attention on natural and bioavailable sources of antioxidants and anti-inflammation, we undertook to investigate the antioxidant and anti-inflammatory properties of Cirsium japonicum var. ussuriense used as a folk medicine in Korea. Methods: The extracts of the leaves, stems, flowers, seeds and roots from C. japonicum var. ussuriense were prepared by extracting with water or 80% ethanol. Total flavonoids and polyphenols were measured by a colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The level of prostaglandin $E_2$ ($PGE_2$) was measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western blot analysis. Results: Total flavonoid and polyphenol amounts of the leaves (CLE) and flowers (CFE) showed higher than those of the seed extract (CSE), stem extract (CSTE) and roots (CRE). CLE and CFE also showed the high antioxidant activities such as DPPH, NO-like and ABTS radical scavenging activity. An antioxidant activities of these water extracts showed higher than those of 80% ethanol extracts. We investigated the anti-inflammatory effects of CLE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. CLE significantly suppressed the levels of the inflammatory mediators such as NO and prostaglandin $E_2$ ($PGE_2$) in dose dependant. Furthermore, the levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with CLE extract in a dose dependent manner. Conclusions: These results suggest that CLE water extract has a higher anoxidant and anti-inflammatory activity, these properties may contribute to the oxidative and inflammatory related disease care.

Protective Effects of Leaf and Flower Extracts from Cirsium japonicum var. ussuriense on Oxidative Damage in Normal Human Erythrocytes and Plasma (엉겅퀴(Cirsium japonicum var. ussuriense) 잎 및 꽃 추출물이 정상인 적혈구와 혈장의 산화적 손상에 대한 보호효과)

  • Kang, Hyun-Ju;Mok, Ji-Ye;Cho, Jung-Keun;Jeon, In-Hwa;Kim, Hyeon-Soo;Park, Ji-Min;Jeong, Seung-Il;Shim, Jae-Suk;Jang, Seon-Il
    • Korean Journal of Pharmacognosy
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    • v.43 no.1
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    • pp.66-71
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    • 2012
  • Cirsium japonicum var. ussuriense is often used in treatment of human disease such as hemorrhage, blood congestion and inflammation. This study was accomplished to evaluate the antioxidant properties of the leaf (CLE) and flower (CFE) extracts of C. japonicum var. ussuriense to protect normal human red blood cells (RBC) and plasma samples against oxidative damage in vitro. CLE and CFE were prepared by extracting with hot water. In red blood cells and plasma, oxidative hemolysis and lipid peroxidation induced by the aqueous peroxyl radical generator [2,2'-azobis (2-amidinopropane) dihydrochloride, AAPH] were significantly suppressed by CLE or CFE in a dose-dependent manner at the same time. CLE and CFE also prevented the depletion of cytosolic antioxidant glutathione (GSH) in RBC. These results suggest that the leaves and flowers of C. japonicum var. ussuriense may have the antioxidant properties.

Analysis of geotechnical Seismic Sensitivity in Kyeongju (경주 지역의 지반공학적 지진 민감도 분석)

  • 선창국
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2000.04a
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    • pp.130-140
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    • 2000
  • The earthquake hazard has been evaluated for 10km by 10km area around Kyeongju which is located near Yangsan fault and has abundant historical earthquake records. The ground motion potentials were determined based on equivalent linear analysis by using the data obtained from in situ and laboratory tests and the El centro eartqhuake record scaled to CLE and OLE of the region. The in situ tests include 9 boring investigations 2 crosshole 7 downhole 13 SASW tests and in the laboratory X-ray diffraction analyses and resonant column tests were performed. The peak ground accelerations range between 0.140g and 0.286g on CLE and between 0.051g and 0.116g on OLE respectively showing the good potential of amplification in the deep alluvial layer which is common in Kyeongju area. the response spectrum based on the Korea design guide was sometimes underestimate the motion. particularly near the natural period of the site and the importance of site-specific analysis and need for the improved site categorization method were introduced.

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The Improvement of skin barrier function and anti-obesity effect of Codonopsis lanceolata by supercritical carbon dioxide extraction

  • Kim, Bora
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.4
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    • pp.674-681
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    • 2020
  • The root of Codonopsis lanceolata has been used in traditional medicine. This study was conducted to confirm the comparative effect of ethanol solvent extraction (CLE) and supercritical carbon dioxide extraction (CLS) of C. lanceolata roots. CLS had higher antioxidant than CLE. For supercritical co-solvent modified carbon dioxide extraction (CLS), it were extracted at 250 bar 50℃ 150 min at a flow rate of ethyl alcohol 3 mL/min for 90min. In addition, CLS inhibited the adipocyte differentiation of 3T3-L1 cells. When treated with the extract at a concentration of 100 ㎍/mL, the Wnt/β-catenin pathway reporter luciferase activity of HEK 293-TOP cells increased approximately by 3-folds compared to that of the untreated control group. Also, the treatment by CLS (50 ㎍/mL) showed a significant increase of involucrin expression. These results indicate that supercritical carbon dioxide extract of C. lanceolatamay serve as a cosmeceutical agent for improving skin barrier function and the treatment of obesity.

Dressing Poses in Relation to Clothing Thermal Insulation

  • Li, Jun;Zhang, Weiyuan;Liu, Yan
    • Fashion & Textile Research Journal
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    • v.4 no.6
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    • pp.544-549
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    • 2002
  • By the movable thermal manikin developed by China Dong Hua university, the laws of clothing thermal insulation influenced by dressing poses are studied. It is found that $I_a$ on nude thermal manikin has no relation to testing pose as a whole (notable level is 5%), while the change of testing pose influences $I_a$ value on parts of body obviously. The testing result $I_{cle}$ on clothed thermal manikin has relation to testing pose. The $I_{cle}$ value of the whole body in seated pose decreases 20 percent compared with that in standing pose (notable level is 1%). In view of heat transmission theory, the reasons are pointed out based on the knowledge of heat transmission.

Effects of Eucommia ulmoides olivon Ethanol Extract on Lipid Metabolism and Antioxidant Enzyme Activities of Rats Fed High Fat Diet (고지방 식이를 투여한 흰쥐의 지질대사와 항산화 효소 활성에 미치는 두충 에탄올 추출물의 영향)

  • 남상명;강일준;정차권;정명은;함승시;오덕환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.5
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    • pp.796-801
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    • 2002
  • To investigate the effects of Ewomia ulmoides olivon extract on the serum and hepatic lipid status and anti-oxidant enzyme activities, male Sprague Dawley (SD) rats were given high fat diets and Eucomia ulmoides olivon ethanol extracts for 6 weeks. Rats were divided into (our groups consisting the control (C), Eucomia ulmoides oliuon extract fed group (CE), high fat diet group (CL) and the extract and high fat fed group (CLE). Eucomia ulmoides oliuon ethanol extract lowered total cholesterol, but increased HDL-cholesterol and HDL-cholesterol/total cholesterol of the serum compared to the control. On the other hand, the ratio of HDL-cholesterol/total cho-lcsterol (HTR) was increased by 55% (p<0.05). High fat diet significantly increased serum cholesterol contents, but decreased HDL-cholesterol level and HTR (p<0.05). Atherogenic index (AI) of CL group was increased by almost four times of the control (p<0.05). Triglycerides (TG) and phospholipids were also increased by high fat diet. Eucomia ulmoides oliuon ethanol extract decreased the levels of TG (p<0.05) and phospholipids (p<0.05). Although liver antioxidant engyme activities including glutathione sulfur transferase (GST), superoxide dismutase (SOD) and catalase were decreased by high fat diet, those were stimulated by the administration of Eucommia ulmoides olivon ethanol extract.

Relationship between Thermal Insulation and the Combinations of Korean Women's Clothing by Season - Using a Thermal Manikin - (한국 성인 여성의 계절별 의복조합과 보온력과의 관련성 - 써멀마네킨 실험에 의한 -)

  • Choi, Jeong-Wha;Ko, Eun-Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.6 s.165
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    • pp.966-973
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    • 2007
  • The purpose of this study was to examine the correlation between the combination of women's clothing by season and thermal insulation using a thermal manikin. A total of 34 kinds of clothing ensembles were selected based on previous studies(8 types for spring/fall, 7 types for summer, and 19 types for winter). The results were as follows: The thermal insulation of clothing ensembles($I_{cle-total}$) ranged from $0.34{\sim}0.60clo$ for spring/fall, $0.16{\sim}0.37clo$ for summer, and $0.89{\sim}1.35clo$ for winter. The correlation coefficient between the thermal insulation of clothing ensembles and thermal insulation accumulated by the individual garments composing of the clothing ensembles($I_{cle-summed}$) was 0.982(p<0.001). The correlation coefficient between the thermal insulation of clothing ensembles and total clothing layers for the upper body part was 0.750 (p<0.001), for the total clothing weight was 0.978(p<0.001), and for the covering area was 0.776(p<0.001). In conclusion, $I_{cle-total}$ showed higher relationships to the $I_{cle-summed}$ and total clothing weight than to the total clothing layers or surface area covered by clothing.